Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparative Evaluation of Culture Media for Quantification of Lactic Acid Bacteria in Various Dairy Products

  • Eiseul Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Shin-Young Lee (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Yoon-Soo Gwak (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hyun-Jae Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Ik-Seon Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hyo-Sun Kwak (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hae-Yeong Kim (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University)
  • Received : 2023.01.25
  • Accepted : 2023.03.14
  • Published : 2023.03.28
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Abstract

Dairy products are extensively used as carriers of probiotic strains that have potential health benefits. Assessment of the viability of probiotic strains during manufacturing is important to ensure that products meet recommended levels. Hence, the method for accurately quantifying lactic acid bacteria (LAB) in probiotic or dairy products is required. The present study aims to examine the performance of de-Man Rogosa Sharpe (MRS), plate count agar with bromocresol purple (PCA with BCP), and glucose blood liver (BL) agars recommended in the Korea Food Code guidelines for counting LAB. Analysis of the performance of culture media containing 19 lactic acid bacterial species commonly encountered in probiotic and dairy products showed no statistically significant difference between 18 reference strains and three culture media (p > 0.01). Furthermore, the suitability of three culture media was verified for the quantitative assessment of LAB in 25 probiotic and dairy products. The number of LAB in three culture media was determined to be more than 107 colony-forming unit (CFU)/ml for fermented milk products and 108 CFU/ml for condensed fermented milk and probiotic products, indicating that they all satisfied the Korea Food Code guidelines. Moreover, there was no statistically significant difference in the amount of LAB counted in all three culture media, suggesting that they can be used to isolate or enumerate LAB in commercial products. Finally, three culture media will be useful for isolating and enumerating LAB from fermented foods as well as gut microflora.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01662001)" Rural Development Administration. Republic of Korea.

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