Journal of Radiopharmaceuticals and Molecular Probes (대한방사성의약품학회지)

Korean Society of Radiopharmaceuticals and Molecular Probes (대한방사성의약품학회)
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Automated radiochemical synthesis of [18F]FET on TRACERlab FX2N module and its quality control

  • Dong Hyun Kim (Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological & Medical Sciences) ;
  • Eun-bi Shin (Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological & Medical Sciences) ;
  • Iljung Lee (Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological & Medical Sciences) ;
  • Heejung Kim (Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological & Medical Sciences) ;
  • Kyo Chul Lee (Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological & Medical Sciences) ;
  • Kyeong Min Kim (Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological & Medical Sciences) ;
  • Joo Hyun Kang (Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological & Medical Sciences) ;
  • Sang Moo Lim (Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological & Medical Sciences)
  • Received : 2021.12.07
  • Accepted : 2021.12.28
  • Published : 2021.12.30
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Abstract

C-11 Radiolabeled amino acid-based radiopharmaceuticals such as [11C]MET for brain tumor PET imaging have limitations due to their short half-life (20 min). F-18 radiolabeled amino acid derivatives have been developed to overcome for the short half-life, one of which is [18F]FET. Brain tumor imaging using [18F]FET showed high uptake in tumor region and no non-specific uptake in inflammatory tissue, which was useful in discriminating the difference between inflammation and tumor especially. In this study, [18F]FET was synthesized using an automatic synthesis module and quality tests were carried out including enantiomeric purity analysis with reference compounds. Radiochemical yield was 50.3 ± 4.9% (n=7, decay-corrected) with molar activity of 76 ± 17 GBq/mmol. The radiochemical purity of >99%. Enantiomeric purity of [18F]FET using chiral HPLC analysis showed >99%, which was confirmed by co-injection with the L-FET and D-FET authentic standards. [18F]FET was prepared with high radiochemical yield and molar activity including no racemate mixture.

Keywords

Acknowledgement

This study was supported by a grant of the Korea Institute of Radiological and Medical Sciences (KIRAMS), funded by Ministry of Science and ICT (MSIT), Republic of Korea. (No. 50539-2021)

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