• 대한가정학회지
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• 제31권2호
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• pp.97-109
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• 1993

The purpose of this study is to investigate the perceptional degree of Stress on family life events, and find out that family enviornment variables and individual resources among the family resources have effected on Stress. For the data, 316 mothers who first child is preschool were selected. The data were analysed with the spss pc+ program using M, SD, Factor analysis, one-way analysis of variance, Regression. The main results are as follows; First, the perceptional degree of stress is relatively middle. Second, among the family enviornmental variables mother's age, mother's educational level, father's educational level, mother's job have influence on the stress. Third, among the individual variables health, self-esteem have on the stress.

• 대한한의학방제학회지
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• 제24권4호
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• pp.323-333
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• 2016

Objectives : The purpose of this study was to investigate musk deer in taxonomic status and genus species. We investigated the characteristic of musk deer for medicinal usages. Methods : To identifications of musk deer on the taxonomic status and genus species, the literary investigation were conducted on the Korean, China, and Japan pharmacopoeia and published herbal books, CITES Species Lists too. Results : Musk deer placement in a separate family status is the Moschidae. M. chrysogaster Hodgson and M. sifanicus Przewalski was the same species. So, Alpine musk deer revised M. chrysogaster Hodgson[=M. sifanicus Przewalski]. Geographic distribution of M. moschiferus L. divided Sibirica group and himalaica group. Group himalaica contains three subspecies: Korean musk deer(M. moschiferus parvipes Hol.), Chinese musk deer(M. chrysogaster Hodgson), and Himalayan musk deer(M. leucogaster Hodgson). The genetic divergence between M. moschiferus L. and other species was clearly distinguished from the others. M. berezovskii Flerove was less than the others. However, the divergence among M. chrysogaster Hodgson, M. fuscus Li, and M. leucogaster Hodgson were quite low. Musk deers are mostly distributed around the high-plateau. Moschus were from Nepal, Bhutan, Russia, China. Forest musk deer(M. berezovskii Flerove) farming was conducted in China from 1950s. In the Korean hebal pharmacopoeia, Moschus include l-muscone($C_{16}H_{30}O$ : 238.40) over 2% for quantitative test. Conclusions : There are three species of musk deer, Siberian musk deer(M. moschiferus L.), forest musk deer(M. berezovskii Flerove), and Alpine musk deer(M. chrysogaster Hodgson) for medicinal usages.

• 대한의생명과학회지
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• 제10권2호
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• pp.163-169
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• 2004

Worldwide, tuberculosis remains one of the leading infectious diseases, accounting for nearly 3 million deaths and more than 8 million new cases annually. DNA typing of Mycobacterium tuberculosis is important for the control of tuberculosis, since it can be used to track transmission route of tuberculosis, source of internal laboratory contaminations, and to answer questions on the nature of tuberculosis infections such as reactivation or exogenous reinfection of disease. At present, IS6110-based RFLP is the choice of method for typing large numbers of clinical isolates of M. tuberculosis, since it has the highest resolution power. However, RFLP requires long time, high cost and qualified experts, so only reference level laboratories can use the RFLP technique. In order to have an optional molecular typing method suitable for the clinical settings, this study evaluated the use of one of PCR-based typing methods, IS6110-based outward PCR for typing clinical isolates of M. tuberculosis. In brief, the results from this study showed that IS6110-based RFLP is useful to discriminate diverse clinical isolates of M. tuberculosis as well as to identify clinical isolates that belong to the same family or cluster groups that have been previously classified by RFLP analysis. In addition, the banding profiles resulted from IS6110-based outward PCR seemed to represent genomic characteristics of M. tuberculosis, since strains belong to the K-family generated unique band that is not present in any other strains but present only in the genome of K-family strains. The IS6110-based outward PCR was also shown to be useful with DNAs isolated directly from liquid cultures indicating this method can be suitable for typing M. tuberculosis in clinical settings.

• Journal of Microbiology and Biotechnology
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• 제26권9호
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• pp.1650-1656
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• 2016

The SCO0284 gene of Streptomyces coelicolor A3(2) is predicted to encode an α-galactosidase (680 amino acids) belonging to glycoside hydrolase family 27. In this study, the SCO0284 coding region was cloned and overexpressed in Streptomyces lividans TK24. The mature form of SCO0284 (641 amino acids, 68 kDa) was purified from culture broth by gel filtration chromatography, with 83.3-fold purification and a yield of 11.2%. Purified SCO0284 showed strong activity against p-nitrophenyl-α-D-galactopyranoside, melibiose, raffinose, and stachyose, and no activity toward lactose, agar (galactan), and neoagarooligosaccharides, indicating that it is an α-galactosidase. Optimal enzyme activity was observed at 40℃ and pH 7.0. The addition of metal ions or EDTA did not affect the enzyme activity, indicating that no metal cofactor is required. The kinetic parameters V_max and K_m for p-nitrophenyl-α-D-galactopyranoside were 1.6 mg/ml (0.0053 M) and 71.4 U/mg, respectively. Thin-layer chromatography and mass spectrometry analysis of the hydrolyzed products of melibiose, raffinose, and stachyose showed perfect matches with the masses of the sodium adducts of the hydrolyzed products, galactose (M+Na, 203), melibiose (M+Na, 365), and raffinose (M+Na, 527), respectively, indicating that it specifically cleaves the α-1,6-glycosidic bond of the substrate, releasing the terminal D-galactose.

• 한국응용과학기술학회지
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• 제37권1호
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• pp.7-16
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• 2020

콩과는 전 세계적으로 약용식물로 흔히 사용된다. 본 연구에서는 약용식물이자 다양한 용도로 사용되는 염료식물인 콩과에 속하는 황기(Astragalus membranaceus), 소목(Caesalpinia sappan L.), 감초(Glycyrrhiza uralensis F.), 갈근 및 갈화(Pueraria lobate O.), 자단향(Pterocarpus santalinus L.) 추출물을 이용하여 항산화능 및 세포 보호능을 비교하고자 한다. 염료 추출물은 라디칼 소거능, 총 페놀 함량 및 MTT assay를 활용한 간세포 보호능이 확인되었다. 소목(5 mg/mL) 추출물에서 93.49%의 가장 높은 라디칼 소거능을 확인하였으며, 황기(5 mg/mL) 추출물에서 7.83%의 가장 낮은 라디칼 소거능을 확인하였다. 또한 소목 추출물의 총 페놀 함량은 310.93 mg GAE/g extract로 확인되며, 가장 높은 총 페놀함량을 확인하였고, 황기 추출물의 총 페놀 함량은 15.33 mg GAE/g extract로 확인되며 가장 낮은 총 페놀 함량을 확인하였다. 게다가, 가장 높은 항산화능을 나타낸 소목(100 ㎍/mL) 및 자단향(100 ㎍/mL) 추출물에서 산화적 스트레스에 대한 세포 보호능을 확인하였다. 이러한 결과 콩과 5종 물질 중 적색 천연색소를 가지는 소목 및 자단향에서 높은 항산화능이 있는 것으로 확인되었다.

• 지역사회간호학회지
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• 제10권2호
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• pp.494-507
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• 1999

This study has developed the sex education contents including new natural family plan, concept and principle by receiving the opinions and advices from professionals and professors on sex education, by amending and supplementing existing teaching materials on sex, and examining the books related with sex education contents that reflect the request of teachers and students, and the lack of sex education teaching materials which have been the reason that needs of school sex education has been highly recognized but not vitalized. The developed sex education contents are 8 areas such as knowing my body right, caring my body, observing the ability of being pregnant, marriage, sexual intercourse, process of pregnancy, miscarriage, and sexual violence, 30 lower areas, and 105 detailed contents in their composition, and here are the results of analyses of the degree of request of nurse teachers and the students on them. The teachers showed highest request in the area of sexual violence(M =4.67), the next was my body care(M=4.44), knowing my body right(M =4.38), process of pregnancy(M=4.13), marriage(M=4.11) and sexual intercoursen (M=3.98), ways of judging the ability of being pregnant(M=3.88), and miscarriage(M=3.72) showed comparative higher request than normal. Not like the teachers, the students showed that they requested Marriage area as the highest need(M=3.04) and next was sexual violence(M=3.02), caring my body(M=3.00) to have been higher than normal. Process of pregnancy(M=2.99), Sexual intercourse(M=2.81), miscarriage(M = 2.77), observation of the ability of being pregnant(M=2.74), and knowing my body right(M=2.70) have been the requests lower than normal. This study showed that sexual violence, marriage, caring my body, were the items most requested by both the leacgers and students and in elementary school the development of sex education teaching materials on natural family plan suitable to the level of development of the children has been requested as it has had no inclusion of the concept of natural family plan, and the students in elementary school are concerned on indirect sexual contents related with physical health like regular exercise rather than direct sexual education contents including sexual intercourse. All these show that they are still so naive on sex. The sense of the teachers on sex has been connected with the level of achievement of sex education of the children as it has been the important cause of deciding the contents of sex education. Therefore the materials developed onesidedly by leaders without reflecting the needs or the preference of the students might be worries of bringing shameful. low quality and negative opinions on sex, and on the other hand the children might lose the concerns and interest on sex education, So the materials should be developed to have the contents of sex education coinciding with the request of the children.

• 한국발생생물학회지:발생과생식
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• 제12권3호
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• pp.297-303
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• 2008

본 논문은 인공적인 단백질인 MCL-1ES BH3M에 관한 것으로 MCL-1ES BH3M를 과발현시 세포사멸을 유도한다. MCL-1L을 주형으로 재조합 PCR을 통해서 MCL-1ES BH3M를 클로닝하였다. 새롭게 클로닝한 단백질인 MCL-1ES BH3M 단백질은 안정성을 유지하기 위해서 PEST 도메인이 제거되어 있으며, 다른 BCL-2 패밀리 단백질과의 결합을 조절하기 위해서 BH3도메인의 Leu-Arg-Arg-Val-Gly-Asp-Gly 서열을 7개의 Ala 잔기로 인위적으로 돌연변이를 유도하였다. MCL-1ES BH3M를 293T 세포에서 과발현할 경우 세포사멸을 유도하였고, 항-세포사멸 단백질인 MCL-1L을 같이 과발현하더라도 세포사멸을 유도하였다. 또한, 과발현시 Caspase 9과 3를 활성화하였으며 면역염색법을 통해서 MCL-1ES BH3M 과발현시 미토콘드리아에 MCL-1ES BH3M 단백질이 부분적으로 위치하는 것을 확인하였다. 이상의 결과로 MCL-1ES BH3M는 Caspase 9과 3의 활성을 통해서 세포사멸을 유도한다. 결론적으로 본 연구는 세포사멸을 유도하는 새로운 molecule을 클로닝하였고, 이 molecule에 의한 세포사멸 기능을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.9021-9025
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• 2014

Background: Breast cancer (BC) is the most common cancer among females in Turkey. Predictors affecting the breast self-examination (BSE) performance vary in developing countries. Objective: To determine the frequency of BSE performance and predictors of self-reported BSEs among women in the capital city of Turkey. Materials and Methods: This cross-sectional study was conducted on 376 Turkish women using a self-administered questionnaire covering socio-demographic variables and BSE-related features. Results: Of the participants, 78.7% (N=296) reported practicing BSE, whereas 9.5% (N=28) were implementing BSE regularly on a monthly basis, and only 5.7% (N=17) were performing BSE regularly within a week after each menstrual cycle. Multivariate logistic regression modeling revealed that BSE performance was more likely in younger age groups [20-39 years] (p=0.018, OR=3.215) and [40-49 years] (p=0.009, OR=3.162), women having a family history of breast disease (p=0.038, OR=2.028), and housewives (p=0.013, OR=0.353). Conclusions: Although it appears that the rates of BSE performers are high, the number of women conducting appropriate BSE on a regular time interval basis is lower than expected. Younger age groups, family history of breast diseases and not being employed were identified as significant predictors of practicing BSE appropriately. Older age and employment were risk factors for not performing BSE in this sample.

• 한국콘텐츠학회논문지
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• 제12권2호
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• pp.222-231
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• 2012

본 연구는 다문화가족의 남성배우자의 이중문화스트레스 및 의사소통과 결혼만족도와의 관계를 파악하고자 전국에 있는 결혼이민자 지원센터, 건강가정지원센터의 프로그램 참가자와 지역사회복지관의 다문화 프로그램을 이용하는 결혼이주여성의 배우자를 대상으로 실시하였다. 첫째, 이중문화스트레스는 하위영역 중 문화충격(M= 4.32)이 가장 높고, 지각된 적대감의 평균값이 가장 낮은 것으로 조사되었는데, 점수가 높을수록 이중문화스트레스가 높은 것으로 볼 수 있다. 둘째, 이중문화스트레스에 따른 결혼만족도는 이중문화스트레스가 높은 집단이 낮은 집단보다 높았다. 셋째, 의사소통에 따른 결혼만족도는 의사소통이 높은 집단이 낮은 집단보다 높았다. 넷째, 의사소통은 이중문화스트레스가 결혼만족도에 미치는 영향을 매개함을 알 수 있다.

• 한국조리학회지
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• 제15권1호
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• pp.16-29
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• 2009

최근 패밀리 레스토랑은 서로 간에 경쟁뿐만 아니라 신규 외식업체의 등장으로 인하여 레스토랑의 경쟁 우위 전략 일환으로 메뉴 품질 향상이 절실히 요구되고 있다. 메뉴의 질적 향상은 고객과 레스토랑 경영에 있어서 매우 중요함에도 불구하고 기존의 틀을 벗어나지 못하고 독창성이 결여된 메뉴를 사용하기 때문에 많은 문제점을 가지고 있는 실정이다. 따라서 본 연구는 서울과 광주 지역에 소재하고 있는 패밀리 레스토랑인 O, T, V, B, M을 선정하여 총 199부의 설문지를 통계처리하여 메뉴 품질이 고객 만족과 신뢰, 재방문 의도에 미치는 영향 관계를 규명하였다. 첫째, 메뉴 품질은 고객 만족에 유의한 영향을 부분적으로 미치는 것으로 나타났다. 둘째, 메뉴 품질은 고객 신뢰에 유의한 영향을 부분적으로 미치는 것으로 나타났다. 셋째, 고객 만족은 재방문 의도에 유의한 영향을 미치는 것으로 나타났다. 넷째, 고객 신뢰는 재방문 의도에 유의한 영향을 미치는 것으로 나타났다. 고객들이 지각하는 패밀리 레스토랑 메뉴 품질 이미지가 고객 만족, 신뢰, 재방문 의도에 긍정적으로 작용할 수밖에 없으므로, 향후 패밀리 레스토랑 기업들이 치열한 경쟁에서 살아남기 위해서는 차별화된 메뉴 품질 향상이 요구된다는 점에서 중요한 의의를 줄 수 있을 것이다.