• Journal of Plant Biotechnology
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• 제33권2호
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• pp.75-84
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• 2006

Heteroplasmic rice plastid transformant was generated using suspension cells as bombardment materials. PCR analyses confirmed incorporation of aadA and cp4-epsps genes into the rice plastid genome by homologous recombination events via the flanking sequences of the trnI and trnA. Transplastomic calli were actively proliferated when cultured on AAM2 medium supplemented with various concentrations (500-3000 mg/L) of streptomycin in dark condition, and transplastomic suspension cells showed resistance to nonselective herbicide, glyphosate. Through 'agarose pie selection' method, heteroplastomic calli, containing considerably high level of transplastome and expressing the CP4 EPSPS protein, were obtained. They were further regenerated to green shoots with healthy roots.

• Journal of Microbiology
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• 제45권3호
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• pp.272-274
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• 2007

During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type $metallo-{\beta}-lactamase$ gene and PER-1 type extended spectrum ${\beta}-lactamase$ gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying $bla_{VIM-2},\;bla_{PER-1}$, and armA genes.

• 대한수의학회지
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• 제25권1호
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• pp.61-67
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• 1985

One hundred and twenty seven strains of Gram-negative rods (72 E. coli, 45 Klebsieila pneumoniae, 8 Enterobacter spp. and 2 Pseudomonas aeruginosa) isolated from bovine mastitis were examined for resistance to ampicilin, carbenicillin and cefazolin, ${\beta}$-lactamase activity and transferable ${\beta}$-lactamase plasmids. Stains resistant to ampicillin were 13.9% in E. coli, 93.3% in Klebsiella pneumoniae, 87.5% in Enterobacter. spp. and all in Pseudomonas aeruginosa, Resistance of E. coli, Klebsiella pneumoniae and Enterobacter spp. to ampicillin was due to the ${\beta}$-lactamases, but all Pseudomonas aeruginosa exhibited a high level of the non-enzymic resistance. Transferable plasmid-mediated ${\beta}$-lactamase synthesis was demonstrated in 61.9% of Klebsiella pneumoniae, 50% of E. coli and 42.9% of Enterobacter spp. The same ${\beta}$-lactamase plasmids specified different resistance levels to various ${\beta}$-lactam antibiotics in different recipients.

• Earthquakes and Structures
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• 제13권4호
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• pp.365-375
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• 2017

Seismic risk management has two main technical aspects: to recommend the construction of high-performance buildings and other structures using earthquake-resistant designs or evaluate existing ones, and to prepare emergency plans using realistic seismic scenarios. An overview of seismic risk assessment methodologies in Croatia is provided with details regarding the components of the assessment procedures: hazard, vulnerability and exposure. For Croatia, hazard is presented with two maps and it is expressed in terms of the peak horizontal ground acceleration during an earthquake, with the return period of 95 or 475 years. A standard building typology catalogue for Croatia has not been prepared yet, but a database for the fourth largest city in Croatia is currently in its initial stage. Two methods for earthquake vulnerability assessment are applied and compared. The first is a relatively simple and fast analysis of potential seismic vulnerability proposed by Croatian researchers using damage index (DI) as a numerical value indicating the level of structural damage, while the second is the Macroseismic method.

• 정보보호학회논문지
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• 제23권1호
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• pp.11-18
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• 2013

실행코드의 변조와 역분석 방지를 위해 단순히 선행블록에서 암, 복호화 키를 얻던 기존의 기법과 달리, 암호학적 MAC함수를 이용한 암호화기법과 코드 블록 간 중요도에 따라 상관관계를 설정하고 상관관계에 따라 암, 복호화 키를 생성하는 새로운 변조 방지 기법을 제안한다. 본 논문에서는 기존의 해쉬함수 대신 암호학적 MAC함수를 사용하고 MAC함수의 키를 동적으로 생성하는 방법을 소개한다. 또 단순히 선행블록의 해쉬 값에서 키를 얻는 것이 아니라 실행 코드 블록을 중요도에 따라 중요도 높음, 중간, 낮음으로 분류하고 중요도 높음 블록은 암호화하고 중요도 중간 블록은 중요도 높음 블록의 키를 생성시키는 블록으로 분류한다. 또 중요도 낮음 블록은 아무 처리도 하지 않음으로 소프트웨어 효율성을 고려한다. 기존 해쉬함수 대신 동적으로 생성되는 링크키를 이용한 MAC함수와 블록상관관계를 함께 사용함으로써 공격자의 분석을 어렵게 한다.

• Tribology and Lubricants
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• 제30권4호
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• pp.240-245
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• 2014

Low alloy heat resistant brake disk and sintered brake pad are applied to mechanical brake system for the speed-up of urban rapid transit. In this research, we analyzed how the frictional characteristics between brake disk and pad are influenced by the disk mass. At a high disk mass, the friction stability was the lower value as a result of the lack of tribofilm formation at the disk surface. Wear rates of friction materials showed the higher value at a low disk mass and wear rates of 10 mm and 15 mm showed the similar level. Average friction coefficient was the lower value at the 10 mm disk thickness and range of variation of average friction coefficient was also the smaller value at the 10 mm disk thickness. However, there were no significant changes in the friction coefficients under any of test conditions. Surface roughness of a disk showed the highest value at the 5 mm disk and surface roughnesses of 10 mm and 15 mm showed the similar level. As a result, friction characteristics of disk mass influenced the friction stability, as well as the wear rate of friction pad and disk, but not the friction coefficient.

• Asian-Australasian Journal of Animal Sciences
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• 제17권5호
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• pp.719-725
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• 2004

Mesquite or Vilayati babul (Prosopis juliflora) is a drought resistant, evergreen, spiny tree with drooping branches and a deep laterally spreading root system. It grows in semi-arid and arid tracts of tropical and sub-tropical regions of the world and is spreading because the leaves are unpalatable and animals do not digest its seed. The mesquite has become a major nuisance; cutting or pruning its branches to form a canopy would provide shade for travelers, aid harvesting of pods, as well as make available wood for fuel. An average plant starts fruiting by 3-4 years of age and yields annually 10-50 kg pods/ tree, which can be collected from May-June and September-October. Availability of pods worldwide is estimated to be about 2-4 million metric tonnes. Ripe pods are highly palatable; on dry matter basis they contain 12% crude protein, 15% free sugar, a moderate level of digestible crude protein (7% DCP) with a high level of energy (75% TDN). The pods contain low tannin levels below those toxic to animals. Seeds contain 31-37% protein; pods should be finely ground before feeding to facilitate utilization of the seeds. Mesquite pods could replace costlier feed ingredients such as grain and bran contributing 10-50% of the diet. Phosphorus supplements need to be added when mesquite pod, exceeds 20% of animals' diet.

• Steel and Composite Structures
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• 제23권4호
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• pp.385-397
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• 2017

Currently, CFRP (Carbon Fiber Reinforced Polymer) plate bonding is used quite extensively as a strengthening method. In this technique, a composite CFRP plate or sheet of relatively small thickness is bonded with an adhesion material to steel or concrete structure in order to improve its structural behavior and strength. The sheets or plates do not require much space and give a composite action between the adherents. In this study, the rotation capacity of CFRP-strengthened cold-formed steel (CFS) beams has been evaluated through numerical investigation. Studies on different structural levels have been performed. At the beam level, C-section has been adopted with different values of profile thickness, web height, and flange width. At the connection level, a web bolted moment resistant type of connection using through plate has been adopted. In web-bolted connections without CFRP strengthening, premature web buckling results in early loss of strength. Hence, CFRP sheets and plates with different mechanical properties and geometric configurations have been examined to delay web and flange buckling and to produce relatively high moment strength and rotation capacity. The numerical results reveal that CFRP strengthening may increase strength, initial stiffness, and rotation capacity when compared with the case without strengthening.

• Wind and Structures
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• 제32권5호
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• pp.405-421
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• 2021

To gain insight into the wind-induced safety concerns associated with attached tower cranes during the construction of super-tall buildings, a 606 m level frame-core tube super-tall building is selected to investigate the wind-induced vibration response and fragility of an outer-attached tower crane at all stages of construction. The wind velocity time history samples are artificially generated and used to perform dynamic response analyses of the crane to observe the effects of wind velocity and wind direction under its working and non-working resting state. The adverse effects of the relative displacement response at different connection supports are also identified. The wind-resistant fragility curves of the crane are obtained by introducing the concept of incremental dynamic analysis. The results from the investigation indicate that a large relative displacement between the supports can substantially amplify the response of the crane at high levels. Such an effect becomes more serious when the lifting arm is perpendicular to the plane of the connection supports. The flexibility of super-tall buildings should be considered in the design of outer-attached tower cranes, especially for anchorage systems. Fragility analysis can be used to specify the maximum appropriate height of the tower crane for each performance level.

• Journal of the Korean Physical Society
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• 제73권10호
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• pp.1473-1478
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• 2018

In this study, we have developed a simple and cost-effective method to prevent edge bead formation by covering the edge of a chip-level substrate with heat-resistant tape during patterning using SU-8. Edge beads are a fundamental problem in photoresists and are particularly notable in high-viscosity fluids and thick coatings. Edge beads can give rise to an air gap between the substrate and the patterning mask during UV exposure, which results in non-uniform patterns. Furthermore, the sample may break since the edge bead is in contact with the mask. In particular, the SU-8 coating thickness of the chip-level substrates used in MEMS or BioMEMS may not be properly controlled because of the presence of edge beads. The proposed method to solve the edge bead problem can be easily and economically utilized without the need for a special device or chemicals. This method is simple and prevents edge bead formation on the sample substrate. Despite the small loss in the taping area, the uniformity of the SU-8 coating is improved from 50.9% to 5.6%.