• 한국가축번식학회지
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• 제7권2호
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• pp.57-71
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• 1983

1. Diluted chicken semen can be preserved at 2 to 5$^{\circ}C$ for 24 to 48 hr with resultant fertility of greater than 90% of that of fresh semen. Turkey semen can be preserved at 10 to 15$^{\circ}C$ for 6 to 24 hr and provide economical fertility. 2. Frozen chicken semen has given variable results; a 21 to 93% fertility ranges as compared to 92 to 94% expected with fresh semen. Highest fertility levels obtained with frozen turkey semen intravaginally inseminated have been 61 and 63% using DMSO and glycerol, respectively, as cryoprotectants. 3. The use of glycerol as a cryoprotectant reauires that its concentration in semen be reduced to less than 2% either by dialysis or centrifugation after thawing and before intravaginal insemination if optimal fertility is to be obtained. 4. The temperature at which cryoprotectants are added to semen and the time allowed for equilibration are important for subsequent fertility pre- and post-freezing. 5. The type of container used for packaging the semen, freeze or cooling rates, thaw rates and level of cryoprotectant all interact in affecting cell survival. 6. Plastic freeze straws as a packaging device for semen offers the following advantages: easy to handle, require minimal storage space, offer a wide range of freeze and thaw rates, and insemination can be made directly from them upon thawing. 7. Controlled slow cooling rates of 1 to 8$^{\circ}C$/min have thus far provided the best results for cooling chicken semen throught the transition phase change (liquid to solid) or critical temperature range of ＋5 to -20 or -35$^{\circ}C$. 8. Highest fertilities have been achieved with frozen chicken semen where a slow thaw rate (2。 to 5$^{\circ}C$) has been used regardless of the freeze rate. 9. To maintain a constant high level of fertility throughout a breeding season with frozen semen, a higher absolute number of spermatozoa must be inseminated (2 to 3 times as many) as compared to fresh semen since a, pp.oximately 50% are destroyed during processing and freezing. 10. The quality of semen may vary with season and age of the male. Such changes in sperm quality could be accentuated by storage effects. Thus, the correct number of spermatozoa may very well vary during the course of a breeding period. 11. As to time of insemination, it is best to avoid inseminating chicken hens within 1-2 hr after or 3-5 hr before oviposition; and turkey hens during or 7-10 hr before oviposition. 12. The physiological receptiveness of the oviduct at the time of insemination is a very important biological factor influencing fertility levels throughout the breeding season.

• 한국발생생물학회지:발생과생식
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• 제20권3호
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• pp.219-225
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• 2016

Most of the commercial devices for vitrification are directly immersed into the warming solution (WS) for increasing of warming rate. However, the previous modified cut standard straw (MCS) which has reported is difficult to immerse into the WS. The aim of this study was to investigate whether the long cut straw (LCS) could be useful as a stable tool for vitrified-warmed human blastocysts. A total of 138 vitrified-warmed cycles were performed between November 2013 and November 2014 (exclusion criteria: women ${\geq}38$ years old, poor responder, surgical retrieval sperm, and severe male factor). The artificial shrinkage was conducted using 29-gauge needles. Ethylene glycol and dimethyl sulfoxide (7.5% and 15% (v/v)) were used as cryoprotectants. Freezing and warming were conducted using the LCS tool. The cap of LCS was removed using the forceps in the liquid nitrogen ($LN_2$) and then directly immersed into the first WS for 1 min at $37^{\circ}C$ (1 M sucrose). Only re-expanded blastocysts were transferred after it was cultured in sequential media for 18-20 h. A total of 294 blastocysts were warmed, and all were recovered (100%). Two hundred eighty-five embryos were survived (96.9%). The vitrified-warmed blastocysts of all patients were transferred without any cancellation. We were able to achieve a reasonable implantation (24.2%), following by clinical pregnancy (36.2%), which then continued to ongoing pregnancy (36.2%), and live birth (31.2%). Using LCS is achieved the acceptable rates of survival, pregnancy and live birth. Therefore, the LCS could be considered as a stable and simple tool for human embryo vitrificaton.

• 한국가금학회지
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• 제41권1호
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• pp.21-27
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• 2014

본 연구는 닭 정액의 동결 보존을 위하여 비 글리세롤성 동결 보호제 중 MA 농도가 정자의 생존율과 융해된 동결 정자를 인공수정을 실시하여 생산된 수정란의 수정율, 부화율을 조사하고자 실시하였다. 동결 보조제로써 MA의 효율성은 7%, 9% 및 11% 범위에서 동결을 실시하였을 때, 정자의 생존율은 $35.16{\pm}6.12%$, $67.83{\pm}15.3%$ 및 $66.2{\pm}16.3%$로 관찰되었으며, 융해된 정자를 인공수정을 실시하여 생산된 수정란의 수정율은 21.5%, 34.7% 및 25%로 관찰되었으며, 수정된 수정란의 부화율은 100%, 89.5% 및 87.5%로 관찰되었다. 대조군으로써 신선 정액은 수정율이 96.0%로 관찰되었고, 부화율은 92.2%로 관찰되었다. 9% MA를 이용한 간이 동결법으로 생산된 동결 정자를 이용하여 3주간 수정란을 검사하였을 때, 수정율은 비록 35.3%로 관찰되었으나, 부화율은 90.3%로 관찰되었다. 이러한 결과에 따르면, 9% 농도로 MA 동결 보호제를 이용할 경우, 동결 및 융해된 정자를 이용하여 생산된 수정란에서 수정율을 감소시킬 수 있음을 보여주고 있으며, 부화율에는 영향을 주지 않음을 추정할 수 있다. 그러므로 수정율과 부화율에 나쁜 영향을 미치지 않고 가금 유전자원의 보존에 중요한 요인이 될 수 있는 적절한 농도의 MA 동결 보호제 범위는 7~9% 농도로 추정된다.

• 한국수정란이식학회지
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• 제29권1호
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• pp.83-90
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• 2014

The main purpose of this study is to estimate the effect of adding Tea-N-Tris (TES) to the freezing buffer for miniature pig sperm. In particular, we attempted to identify the association between the MMPs expression and the fertility and viability of frozen sperm from each extender (LEY (Lactose Egg-Yolk), TLE (TES + LEY), TFGE (TES + Fructose + Glucose Egg-Yolk)). In accordance with this, Hypoosmotic Swelling Test (HOST) respond test was the lowest among sperms frozen in LEY while the highest HOST respond was observed among sperms frozen in TLE. Furthermore, we observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. The expression of MMP-9 and MMP-2 was the highest in sperms frozen in LEY, Meanwhile, sperms from the TFGE and TLE group showed lower level of MMP-9 and MMP-2 expression in the order of TLE being the lowest. LEY group showed lower rate of blastocyst development than the TES supplement group, although the difference was not statistically significant. Meanwhile the rate of blastocyst development appeared similar when sperms from TLE and TFGE group were used for IVF. Together, these results indicate that adding Tea-N-Tris to the sperm freezing buffer only suppresses MMPs protein activation but also maximize in-vitro fertility, providing a means to improve the success rate in the in vitro manipulation of miniature pig sperm.

• 한국토양비료학회지
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• 제15권3호
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• pp.194-198
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• 1982

1974년(年)부터 1981년(年)까지의 연초경작지별(煙草耕作地別) 각종(各種) 비료시험성적(肥料試驗成績)을 수집(蒐集), 정리(整理)하여 비옥도(肥沃度)가 상이(相異)한 경작지토양(耕作地土壤)에서의 무비구 수량별(收量別) 표준시비량(標準施肥量)(10-15-20, 125kg/10a) 시비시(施肥時)의 잎담배 수량(收量) 및 품질(品質)과의 관계를 검토(檢討)하였다. 무비구 수량(收量)이 높은 (비옥도(肥沃度)가 높은) 토양(土壤)일수록 수량(收量)은 대수곡선적인 증가를 보이나 품질(品質)은 저하(低下)하는 유의성(有意性)있는 상관을 보였다. 표준시비량(標準施肥量)이 적용(適用)되는 적정비옥도(適正肥沃度)는 무비구 수량(收量)이 81kg/10a되는 비옥도(肥沃度)를 갖는 토양(土壤)이며, 무비구 수량(收量)이 이보다 많은 경작지(耕作地)에서는 현시비추천량(現施肥推薦量)이 적량인 것으로 판단된다. 따라서 무비구 수량(收量)을 기준(基準)으로 적정시비량(適正施肥量)을 추정(推定)할 수 있을 것으로 기대(期待)되며, 더 나아가서 무비구 수량(收量)을 지배(支配)하는 환경요인(環境要因) 및 토양이화학적(土壤理化學的) 요인(要因)이 밝혀지면 이 인자(因子)로써 적정시비량(適正施肥量)을 추천(推薦)할 수 있는 평가방법이 구명(究明)될 것이다.

• Clinical and Experimental Reproductive Medicine
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• 제51권1호
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• pp.75-84
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• 2024

Objective: The purpose of this study was to evaluate the impact of preimplantation genetic testing for aneuploidy (PGT-A) on clinical outcomes among high-risk patients. Methods: This retrospective study involved 1,368 patients and the same number of cycles, including 520 cycles with PGT-A and 848 cycles without PGT-A. The study participants comprised women of advanced maternal age (AMA) and those affected by recurrent implantation failure (RIF), recurrent pregnancy loss (RPL), or severe male factor infertility (SMF). Results: PGT-A was associated with significant improvements in the implantation rate (IR) and the ongoing pregnancy rate/live birth rate (OPR/LBR) per embryo transfer cycle in the AMA (39.3% vs. 16.2% [p<0.001] and 42.0% vs. 21.8% [p<0.001], respectively), RIF (41.7% vs. 22.0% [p<0.001] and 47.0% vs. 28.6% [p<0.001], respectively), and RPL (45.6% vs. 19.5% [p<0.001] and 49.1% vs. 24.2% [p<0.001], respectively) groups, as well as the IR in the SMF group (43.3% vs. 26.5%, p=0.011). Additionally, PGT-A was associated with lower overall incidence rates of early pregnancy loss in the AMA (16.7% vs. 34.3%, p=0.001) and RPL (16.7% vs. 50.0%, p<0.001) groups. However, the OPR/LBR per total cycle across all PGT-A groups did not significantly exceed that for the non-PGT-A groups. Conclusion: PGT-A demonstrated beneficial effects in high-risk patients. However, our findings indicate that these benefits are more pronounced in carefully selected candidates than in the entire high-risk patient population.

• Animal Bioscience
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• 제37권2호
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• pp.210-217
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• 2024

Objective: This study investigated the efficacy of different concentrations of gelatin supplementation in long-term semen extender on boar semen quality during storage for 10 days at 17℃. Additionally, oxytocin was added to stored semen to enhance fertility. Methods: In Experiment 1, boar semen was collected, diluted with gelatin at concentrations between 0% and 2.5% (w/v) and mixed with a semen extender. Then, it was kept in a refrigerator at 17℃ and stored for 10 days. In Experiment 2, the sperm quality was examined after adding 0, 5, and 10 IU oxytocin per artificial insemination dose to the most effective semen extender from Experiment 1 and placing it in a refrigerator at 17℃ for 10 days. In Experiment 3, the fertility potential in terms of non-return rate and litter size was determined using the most effective solid-stored semen supplemented with oxytocin. Results: The results indicated that sperm quality decreased with increasing storage time (p<0.05). The sperm quality in terms of total motility, progressive motility, and viable sperm with intact acrosomes and high mitochondrial potential was the highest with 1.5% gelatin supplementation (p<0.001) on all days of storage. Treatment with oxytocin did not affect sperm quality (p>0.05). The non-return rate and litter size after insemination with semen supplemented with 1.5% gelatin and 10 IU of oxytocin after 8 to 10 days of storage were comparable to those of the control group (p>0.05). Conclusion: A semen extender as a solid medium supplemented with 1.5% gelatin successfully preserved boar semen for a long storage duration. Treatment with oxytocin did not affect sperm quality. In addition, the fertility capacity using 1.5% gelatin with 10 IU oxytocin and stored for 8 to 10 days was acceptable and comparable to that of short-term storage.

• 한국초지조사료학회지
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• 제31권4호
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• pp.345-352
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• 2011

축산농가의 밭 사료포는 농가에 따라 다량의 축분을 시용하는 경우가 있어 농가마다 사료포장의 토양비옥도 차이가 컸다. 따라서 본 시험은 토양 유기물함량의 차이가 총체맥류의 생육 및 수량에 미치는 영향을 분석하여 토양비옥도에 따라 적합한 총체맥류를 선정코자 하였다. 가. 이삭수는 보통지 대비 비옥지에서 증가되는 비율은 삼한귀리> 청우밀> 곡우호밀> 영양보리> 신영트리티케일 순으로 높았고, 척박지에서 감소되는 비율은 영양보리> 곡우호밀 > 청우밀 > 신영트리티케일 > 삼한귀리 순이었다. 전체적으로 토양비옥도에 따른 이삭수의 변화는 신영트리티케일이 다른 총체맥류에 비하여 변화가 적었다. 나. 척박지에서 총체수량이 높은 총체맥류는 신영트리티케일로서 청보리인 영양보리에 비하여 69% 증수되었다. 다. 신영트리티케일이 다른 총체맥류에 비하여 비옥지, 보통지, 척박지 모두 절대적인 건물 수량과 가소화 건물수량이 높았고, 다음은 청우밀이었으며, 곡우호밀이 가장 낮았다. 라. 토양유기물 함량에 따라 가소화건물수량이 증감에 대한 안정성 측면에서 본다면 비옥지에서는 삼한귀리와 영양보리가 증수효과가 다른 총체맥류에 비하여 크게 나타났고, 척박지에서는 신영 트리티케일과 청우밀에서의 감수율이 다른 총체맥류에 비하여 낮았다.

• 부모자녀건강학회지
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• 제20권2호
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• pp.67-79
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• 2017

Purpose: This research aimed to study the awareness of marriage, childbirth, fertility and high-risk pregnancy among university students. Methods: 250 university students from Seoul and Gyeonggi-do completed questionnaires regarding their awareness on marriage, childbirth, fertility, and high-risk pregnancy. Results: The average age of participants was 22.5. The ideal marriage age was 29.8. 201 (80.4%) participants had an intention of getting married, and 160 (60.4%) answered they should have children. The ideal age of first childbirth was 30.4 and last childbirth was 35.1. There were 126 (50.4%) with prior education regarding high-risk pregnancy, subfertility or infertility. The score of needs for education associated with pregnancy was 7.6 out of 10. The average correct answer rate of awareness of fertility issues was 30.7%. There were significant differences between men and women, 25% of men and 38% of women overestimated the age at women are most fertile. Only 23.7% of men and 25.9% of women knew the correct age when there was marked decrease in women's fertility. Conclusion: Awareness of a lack of marriage, childbirth, fertility, and high-risk pregnancy may influence future family planning and health. These results can be used to plan programs or education for marriage, pregnancy, and childbirth.

• Clinical and Experimental Reproductive Medicine
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• 제38권2호
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• pp.87-92
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• 2011

Objective: This study aimed to determine the safety and clinical effect of artificial shrinkage (AS) in terms of assisted hatching of fresh blastocysts. Also, we evaluated the correlation between patient age and the effect of AS on clinical outcome. Methods: Two AS methods, using a 29-gauge needle and laser pulse, were compared. Seventy-three blastocysts were shrunk using a 29-gauge needle and the same number of other blastocysts were shrunk by a laser pulse. We evaluated the shrunken blastocysts hourly and considered them viable if they re-expanded >70%. Blastocyst transfer cycles (n=134) were divided into two groups: a control group consisted of the cycles whose intact embryos were transferred (n=100), while the AS group consisted of the cycles whose embryos were replaced following AS (n=34). The implantation and pregnancy rates of the control group and AS group were compared ($p$ <0.05). Results: The re-expansion rates of the 29-gauge needle and laser pulse AS groups were similar (56 [76.7%] vs. 62 [84.9%], respectively). All of the remaining shrunken blastocysts were re-expanded within 2 hours. There was no degeneration of shrunken blastocysts. The total and clinical pregnancy rate of the AS group (23 [67.6%]; 20 [58.8%], respectively) was significantly higher than that of the control group (47 [47.0%]; 39 [39.0%], respectively). In the older patient group, there was no difference in the clinical outcomes between the AS and control groups. Conclusion: These results suggest that AS of blastocoele cavity, followed by the transfer, would be a useful approach to improve the clinical outcome in cycles in which fresh blastocyst stage embryos are transferred.