Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of gelatin and oxytocin supplementation in a long-term semen extender on boar semen quality and fertility potential

  • Received : 2023.06.23
  • Accepted : 2023.08.28
  • Published : 2024.02.01
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Abstract

Objective: This study investigated the efficacy of different concentrations of gelatin supplementation in long-term semen extender on boar semen quality during storage for 10 days at 17℃. Additionally, oxytocin was added to stored semen to enhance fertility. Methods: In Experiment 1, boar semen was collected, diluted with gelatin at concentrations between 0% and 2.5% (w/v) and mixed with a semen extender. Then, it was kept in a refrigerator at 17℃ and stored for 10 days. In Experiment 2, the sperm quality was examined after adding 0, 5, and 10 IU oxytocin per artificial insemination dose to the most effective semen extender from Experiment 1 and placing it in a refrigerator at 17℃ for 10 days. In Experiment 3, the fertility potential in terms of non-return rate and litter size was determined using the most effective solid-stored semen supplemented with oxytocin. Results: The results indicated that sperm quality decreased with increasing storage time (p<0.05). The sperm quality in terms of total motility, progressive motility, and viable sperm with intact acrosomes and high mitochondrial potential was the highest with 1.5% gelatin supplementation (p<0.001) on all days of storage. Treatment with oxytocin did not affect sperm quality (p>0.05). The non-return rate and litter size after insemination with semen supplemented with 1.5% gelatin and 10 IU of oxytocin after 8 to 10 days of storage were comparable to those of the control group (p>0.05). Conclusion: A semen extender as a solid medium supplemented with 1.5% gelatin successfully preserved boar semen for a long storage duration. Treatment with oxytocin did not affect sperm quality. In addition, the fertility capacity using 1.5% gelatin with 10 IU oxytocin and stored for 8 to 10 days was acceptable and comparable to that of short-term storage.

Keywords

Acknowledgement

The authors thank Betagro Agro Industry Co., Ltd. for semen samples and the Northeastern Frozen Semen Production Center in Khon Kaen province for semen quality evaluation equipment (CASA).

References

  1. Waberski D, Riesenbeck A, Schulze M, Weitze KF, Johnson L. Application of preserved boar semen for artificial insemination: Past, present and future challenges. Theriogenology 2019;137:2-7. https://doi.org/10.1016/j.theriogenology.2019.05.030
  2. Boe-Hansen GB, Christensen P, Vibjerg D, Nielsen MBF, Hedeboe, AM. Sperm chromatin structure integrity in liquid stored boar semen and its relationships with field fertility. Theriogenology 2008;69:728-36. https://doi.org/10.1016/j.theriogenology.2007.12.004
  3. Nagy SZ, Sinkovics GY, Kovac A. Viability and acrosome integrity of rabbit spermatozoa processed in a gelatin-supplemented extender. Anim Reprod Sci 2002;70:283-6. https://doi.org/10.1016/s0378-4320(01)00189-0
  4. Schulze M, Rudiger K, Waberski D. Rotation of boar semen doses during storage affects sperm quality. Reprod Domest Anim 2015;50:684-7. https://doi.org/10.1111/rda.12532
  5. Menegat MB, Mellagi APG, Bortolin RC, et al. Sperm quality and oxidative status as affected by homogenization of liquid-stored boar semen diluted in short- and long-term extenders. Anim Reprod Sci 2017;179:67-79. https://doi.org/10.1016/j.anireprosci.2017.02.003
  6. Lopez-Gatius F, Sances G, Sancho M, et al. Effect of solid storage at 15 ℃ on the subsequent motility and fertility of rabbit semen. Theriogenology 2005;64:252-60. https://doi.org/10.1016/j.theriogenology.2004.11.015
  7. Yaniz J, Marti JI, Silvestre MA, et al. Effects of solid storage of sheep spermatozoa at 15℃ on their survival and penetrating capacity. Theriogenology 2005;64:1844-51. https://doi.org/10.1016/j.theriogenology.2005.04.012
  8. Kheawkanha T, Chankitisakul V, Thananurak P, Pimprasert M, Boonkum W, Vongpralub T. Solid storage supplemented with serine of rooster semen enhances higher sperm quality and fertility potential during storage at 5℃ for up to 120 h. Poult Sci 2023;102:102648. https://doi.org/10.1016/j.psj.2023.102648
  9. Resseguie WD, Hughes BL, Jones JE, Thurston RJ. An evaluation of gelatin as a diluent component for storage of chicken semen. Poult Sci 1981;60:469-76. https://doi.org/10.3382/ps.0600469
  10. Corcini CD, Moreira F, Pigozzo R, Varel JrAS, Torres NU, Lucia Jr T. Semen quality and reproductive performance after artificial insemination with boar sperm stored in a gelatin-supplemented extender. Livest Sci 2011;138:289-92. https://doi.org/10.1016/j.livsci.2010.11.019
  11. Langendijk P, Soede NM, Kemp B. Uterine activity, sperm transport, and the role of boar stimuli around insemination in sows. Theriogenology 2005;63:500-13. https://doi.org/10.1016/j.theriogenology.2004.09.027
  12. Claus R, Schams D. Influence of mating and intra-uterine oestradiol infusion on peripheral oxytocin concentrations in the sow. J Endocrinol 1990;126:361-5. https://doi.org/10.1677/joe.0.1260361
  13. Padilla L, Lopez-Arjona M, Martinez-Subiela S, Rodriguez-Martinez H, Roca J, Barranco, I. Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows. J Anim Sci Biotechnol 2021;12:101. https://doi.org/10.1186/s40104-021-00620-z
  14. Pena FJ, Dominguez JC, Carbajo M, Anel L, Alegre B. Treatment of swine summer infertility syndrome by means of oxytocin under field conditions. Theriogenology 1998;49:829-36. https://doi.org/10.1016/S0093-691X(98)00032-6
  15. Manjarin R, Kirkwood RN, Ngula J, Martinez-Pastor F, Alegre B, Dominguez JC. Effect of oxytocin, cloprostenol or buserelin in semen doses on sow fertility. Animals (Basel) 2019;9:746. https://doi.org/10.3390/ani9100746
  16. NRC. Nutrition requirements of domestic animal, Nutrient requirements of swine. 10th ed. Washington, DC, USA: National Academy Press; 1998
  17. Ratchamak R, Vongpralub T, Boonkum W, Chankitisakul V. Cryopreservation and quality assessment of boar semen collected from bulk samples. Vet Med (Praha) 2019;64:20916. https://doi.org/10.17221/125/2018-VETMED
  18. Weitze KF. 1991. Long-term storage of extended boar semen. In: Johnson LA, Rath D, Editors. Boar Semen Preservation II. August 1990, Beltsville, MD, USA. Reprod Domest Anim (Supplement), 1990. pp. 231-53.
  19. Ratchamak R, Ratsiri T, Kheawkanha T, Vongpralub T, Boonkum W, Chankitisakul V. Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender. Anim Sci J 2020;91:e13428. https://doi.org/10.1111/asj.13428
  20. Vongpralub T, Thananurak P, Sittikasamkit C, et al. Comparison of effects of different antioxidants supplemented to long-term extender on boar semen quality following storage at 17℃. Thai J Vet Med 2016;46:119-26. https://doi.org/10.56808/2985-1130.2703
  21. Esbenshade KL, Nebel RL. Encapsulation of porcine spermatozoa in poly-lysine microspheres. Theriogenology 1990;33:499-508. https://doi.org/10.1016/0093-691X(90)90507-P
  22. Vyt P, Maes D, Sys SU, Rijsselaere T, Van Soom A. Air contact influences the pH of extended porcine semen. Reprod Domest Anim 2007;42:218-20. https://doi.org/10.1111/j.1439-0531.2006.00733.x
  23. Kaeoket K, Srisowanna T, Wichaidit U, Chanapiwat P, Maneein S. Comparative study on six different long-term commercial extenders for fresh boar semen. Thai J Vet Med 2010;40:25763. https://doi.org/10.56808/2985-1130.2235
  24. Purdy PH, Tharp N, Stewart T, Spiller SF, Blackburn HD. Implications of the pH and temperature of diluted, cooled boar semen on fresh and frozen-thawed sperm motility characteristics. Theriogenology 2010;74:1304-10. https://doi.org/10.1016/j.theriogenology.2010.04.030
  25. Cheng WTK. Preservation of boar semen at 15℃. J Chinese Soc Vet Sci 1988;14:339-50.
  26. Zhou JB, Yue KZ, Luo MJ, et al. Effect of extenders and temperatures on sperm viability and fertilizing capacity of Harbin White boar semen during long-term liquid storage. Asian-Australas J Anim Sci 2004;17:1501-8. https://doi.org/10.5713/ajas.2004.1501
  27. Xu CL, Zhou JB, Zhao BT, et al. Liquid storage of goat semen in chemically defined extenders. Reprod Domest Anim 2009;44:771-8. https://doi.org/10.1111/j.1439-0531.2008.01071.x
  28. Claus R. Physiological role of seminal components in the reproductive tract of the female pig. J Reprod Fertil (Supplement) 1990;40:117-31.
  29. Holt C, Holt WV, Moore HDM, Reed HCB, Curnock RM. Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. J Androl 1997;18:312-23. https://doi.org/10.1002/j.1939-4640.1997.tb01925.x
  30. Tardif S, Laforest JP, Cormier N, Bailey JL. The importance of porcine sperm parameters on fertility in vivo. Theriogenology 1999;52:447-59. https://doi.org/10.1016/S0093-691X(99)00142-9
  31. Bielas W, Nizanski W, Partyka A, Rzasa A, Mordak R. Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters. Acta Vet Scand 2017;59:58. https://doi.org/10.1186/s13028-017-0325-9