Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Impact of In-vitro Fertility and Matrix Metalloproteinases Activation of Spermatozoa by Supplement of Tea-N-Tris to Sperm Cryopreservation of Miniature Pig

미니돼지 정자 동결 보존에 Tea-N-Tris의 첨가가 체외 수정 및 MMPs 활성에 미치는 영향

  • Kim, Sang-Hwan (Institute of Genetic Engineering, Hankyong National University) ;
  • Kang, Hyun-Ah (Major in Animal Fusion Biotechnology, Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Park, Yong-Su (Jangsu Endangered Species Restoration Team) ;
  • Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
  • 김상환 (한경대학교 유전공학연구소) ;
  • 강현아 (한경대학교 미래융합기술대학원 동물바이오융합전공) ;
  • 박용수 (장수군청 환경위생과 생태복원팀) ;
  • 윤종택 (한경대학교 유전공학연구소)
  • Received : 2014.03.12
  • Accepted : 2014.03.26
  • Published : 2014.03.31
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Abstract

The main purpose of this study is to estimate the effect of adding Tea-N-Tris (TES) to the freezing buffer for miniature pig sperm. In particular, we attempted to identify the association between the MMPs expression and the fertility and viability of frozen sperm from each extender (LEY (Lactose Egg-Yolk), TLE (TES + LEY), TFGE (TES + Fructose + Glucose Egg-Yolk)). In accordance with this, Hypoosmotic Swelling Test (HOST) respond test was the lowest among sperms frozen in LEY while the highest HOST respond was observed among sperms frozen in TLE. Furthermore, we observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. The expression of MMP-9 and MMP-2 was the highest in sperms frozen in LEY, Meanwhile, sperms from the TFGE and TLE group showed lower level of MMP-9 and MMP-2 expression in the order of TLE being the lowest. LEY group showed lower rate of blastocyst development than the TES supplement group, although the difference was not statistically significant. Meanwhile the rate of blastocyst development appeared similar when sperms from TLE and TFGE group were used for IVF. Together, these results indicate that adding Tea-N-Tris to the sperm freezing buffer only suppresses MMPs protein activation but also maximize in-vitro fertility, providing a means to improve the success rate in the in vitro manipulation of miniature pig sperm.

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References

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