• 한국치위생학회지
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• 제24권2호
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• pp.131-139
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• 2024

Objectives: Oral bacterial samples included subgingival, supragingival, and saliva plaques. As the diversity and number of microorganisms deffer depending on the area of the oral cavity and the method used, an appropriate and reliable collection method is important. The present study investigated oral bacterial sampling methods. Methods: Supragingival dental plaque was collected from the buccal and lingual tooth surfaces of study participants using sterilized cotton swabs. Plaques were collected from the subgingival area using a sterilized curette. Bacterial genomic DNA was extracted using MagNA Pure 96 DNA and Viral NA low-volume kits. Real-time polymerase chain reaction (PCR) was performed using the PowerCheck^TM Periodontitis Pathogens Multiplex Real-time PCR kit. Results: Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Fusobacterium nucleatum of the orange complex were not observed in the subgingival biofilms of all study participants. For Porphyromonas. gingivalis, a significant correlation was observed between supragingival, subgingival, and total tooth surface biofilms. Compared to the supragingival and subgingival biofilmss, total tooth surface biofilm exhibited the highest bacterial count when the inswabbing method was used. Conclusions: Based on these findings, the supragingival swab method is recommended for oral bacterial research.

• 방사선산업학회지
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• 제11권1호
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• pp.1-6
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• 2017

Reported some level of bacteria in areas that are well made contact in Radiology imaging room evaluate the importance of cleanliness in the hospital management of equipment to check for the presence of pathogenic bacteria. Gwang-ju and Jeol-la city and medium-sized hospitals in the material with a cotton swab and rub evenly Radiology selection cassette, a handle, Apron of the imaging apparatus having the most contact with patients from July 2016 to August 2016 as a target in place and special studios 6, and saline solution will placed in a test tube containing. The swab sample was diluted 1,000 times, you can see the bacteria and the intestinal bacterial selective medium Trypticase Soy Agar (TSA), Muller-Hinton Agar (MHA), Eosin-Methylene Blue (EMB), ENDO(BD, NJ, USA) then incubated smear to. In the incubator (incubator, SANYO, Japan) was observed after incubation of bacteria and counting the total number of bacteria also Colonies (colony) suspected intestinal bacteria were isolated and cultured on KIA medium (BD, NJ, USA). As a result, it was found that this came Gram positive Coccus A hospital handle the F hospital, from the C Gram positive Coccus cassette and handle the F hospital. The striking yellow coloring Staphylococcus aureus 110 agar (STA 110) in the medium sample, but it is suspected staphylococcal Coccus to the final identification in the laboratory is not a single specimen of the two samples from Gram positive Coccus biochemical identification Identification Kit is an API could not, it was thought to be non-Staphylococcus aureus was cultured on blood agar suggesting that (BAP) blood of dance. Dynamic tests were conducted biochemical API kit of the two samples were identified from Gram positive Coccus bacteria Escherichia coli (E. coli) is F hospital cassette was confirmed Eenterobacter cloaca in A hospital possession. Did not aggregate O-26, O-111, O-157 and the serum test was conducted in the laboratory from the E. coli F cassette hospital.

• Journal of Radiation Protection and Research
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• 제37권4호
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• pp.226-230
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• 2012

방사선 사고시 피폭환자로부터 채취된 스메어 시료의 전알파 방사능 측정법을 수립하였으며 이를 바탕으로 피폭환자의 후속조치 절차를 확립하였다. 국내방사선사고 대응기관에서 사용중인 스메어용 면봉을 이용하여 계측 절차를 검증하였다. 액체섬광계수기를 이용하여 표준선원을 점적한 시료의 계측 결과 20% 이내에서 인증값과 잘 일치하였으며, 채취 조건은 세정제 등을 이용하는 것이 더 높은 스메어 효율을 보였다. 액체섬광계수법 특성상 소광현상의 영향을 배제하기 위해 건조 등의 최소한의 전처리가 필요 할 것으로 판단되었다. 계측결과를 바탕으로 방사선비상시 피폭환자에 대한 의료적 처치 기준 및 선량평가 절차를 수립하였다.

• 보존과학회지
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• 제30권2호
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• pp.157-168
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• 2014

본 연구는 행정박물 보존 처리에서 자주 사용되고 있는 금속 광택제 중 3 종 (Polish T, H, C)을 선정하여 은표면의 황색 변색층을 효과적으로 제거함과 동시에 표면의 손상을 최소화하며, 광택제에 함유된 변색 억제 성분 (tarnish inhibitor)의 성능을 비교하여 보존성이 가장 우수한 광택제를 알아보고자 하였다. 실험 결과, 광택제 C를 사용하여 면봉 및 린트-프리 와이프로 세정한 시편들이 균일한 표면의 표출과 최소의 손상을 가져온다는 결과를 얻었으며 부식층 제거에도 뛰어난 결과를 보임을 확인할 수 있었다. 또한 광택제 처리 후 변색 억제 필름이 형성 되어도 변색 현상을 완벽히 방지할 수 없지만 그 중에서도 광택제 C가 자외선과 황변에 가장 안정한 것으로 나타났다. 접촉각 측정 결과에서도 소수성 및 분산 성질이 광택제 T와 광택제 H보다 상대적으로 우수한 특성을 보였다. 본 실험을 통해 현재 은제 행정박물 보존 처리에 사용되는 광택제 중에서 광택제 C가 황변 및 표면 특성이 가장 우수한 성능을 보임을 확인할 수 있었다.

• 구강회복응용과학지
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• 제30권3호
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• pp.199-205
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• 2014

목적: 치과용 연마기를 통한 캔디다균의 2차 감염성 여부를 알아보고자 하였다. 연구 재료 및 방법: 동일한 모형에서 제작된 상악 총의치를 캔디다균에 감염시키고, 멸균된 퍼미스와 휠 증류수를 이용하여, 치과기공용 연마기로 연마하였다. 이후 멸균된 상악 총의치를 연이어 연마한 뒤연마한 부위를 면봉으로 채취하여, 캔디다균을 배양하였다. 연마한 휠은 3일동안 상온에 노출시켜 24시간 간격으로 캔디다균의 잔존여부를 측정하였다. 결과: 연마한 멸균 총의치에서 다수의 캔디다균이 발견되었으며, 연마한 휠에서는 2일까지 캔디다균이 검출되었다. 결론: 의치 연마를 위한 치과기공용 연마기를 사용시 캔디다균의 감염가능성이 있기 때문에 감염방지책이 필요하다.

• Archives of Plastic Surgery
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• 제34권3호
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• pp.314-318
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• 2007

Purpose: Pressure sore wound develops inevitably in long-term, immobilized and hospitalized patients. Sore wound infection is common problem and makes healing process difficult. We aimed to identify the pathogens of the purulent discharge in sore wound and to obtain information for appropriate antibiotics through a sensitivity test Methods: The bacteriologic study was made on 120 cases of patients who admitted or visited our hospital from 2004 January to 2005 December for sore wound treatment. Culture material was collected in BBL transport media with cotton swab and cultured by MacConkey agar plate. The method of MIC by VITEK and Microscan was used for sensitivity test. Results: Among 120 specimens, organisms were isolated from 77(64.2%) cases. Gram positive organisms were cultured in 73 specimens, Gram negative organisms in 46 specemens, and fungi in 2 specimens. Mixed infection by Gram (+) and Gram (-) bacteria were observed in 34 specimens. Among them, S. aureus was the most common isolate in 24(31.2%) patients and 10 (13.0%) S. Aureus isolates were MRSA. The most prevalent Gram-negative organism was Escherichia coli in 20 patients(25.9%). Vancomycin and teicoplanin showed highest sensitivity to Gram-positive organisms and imipenem and amikacin to Gram-negative organisms. Conclusion: Pressure sore wound demands consideration of multimodal therapeutic aspects and these findings would be useful informations to physicians, nurses and clinical assistants in understanding the nature of sore wound and selecting appropriate antibiotics.

• 대한방사선기술학회지:방사선기술과학
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• 제38권4호
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• pp.395-401
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• 2015

영상의학과의 흉부엑스선 검사에서 감염관리에 대한 기기의 오염 정도를 세균학적으로 확인하고 균을 동정하여 병원감염의 예방과 감염관리방안을 마련하는데 목적이 있다. 영상의학과 흉부 엑스선 검사에서 환자의 기기 접촉부위인 어깨, 손, 턱, 흉부 측면촬영의 손잡이 부위를 70% 이소프로필 알코올로 소독하기 전과 후를 면봉으로 검체를 채취하여 세균을 동정하여 비교하여 분석하였다. 흉부 엑스선 기기에서 소독전의 측면촬영의 손잡이에서 그람 양성의 Staphylococcus가 분리되었다. 최종동정을 위해 노보바이오신 (nobobiocin) 항생제 시험을 실시하여 민감성으로 Staphylococcus epidermidis로 확정하였다. 흉부촬영 기기에서 측면촬영의 손잡이 부위를 소독 전에 Staphylococcus epidermidis 균이 검출되었다. 흉부엑스선 검사에서 기기를 주기적인 소독 및 멸균을 실시하여 병원감염을 예방하는 관리방안을 마련하여야 한다.

• 대한치위생과학회지
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• 제5권1호
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• pp.21-27
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• 2022

Background: This study was conducted to analyze the time for re-detection of bacteria after surface disinfection using wet wipes, isopropyl alcohol, and benzalkonium chloride antibacterial tissue and provide standards for re-execution of surface disinfection with wet and antibacterial tissues. Methods: Seven laptops were wiped with wet tissue and isopropyl alcohol and benzalkonium chloride antibacterial tissues. Test areas were rubbed with a sterile cotton swab at baseline and after 30, 60, and 120 min. After plating on a tryptic soy agar medium, the number of colonies was counted by culturing at 36.5℃ for 24 h. Results: The average number of bacterial colonies was 5.85 ± 4.33 before isopropyl alcohol wiping and nil after wiping. The average number of bacterial colonies was 12.28 ± 14.67 benzalkonium chloride wiping and nil after wiping. Before wiping with wet wipes, the average number of bacterial colonies on laptop surfaces was 3.42 ± 5.22. Bacteria decreased after wiping with wet wipes but increased again over time. Conclusions: Wet wipes can temporarily reduce bacteria but are unsuitable for removing bacteria.

• 약학회지
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• 제52권6호
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• pp.419-425
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• 2008

A qualitative and quantitative analytical method was developed for detection of methamphetamine (MA) and its main metabolite amphetamine (AM) in oral fluid. Oral fluids of eleven drug abusers were provided by Police, specimens were collected by stimulation with a cotton swab treated with 20 mg of citric acid ($Salivette^{(R)}$; Sarstedt, USA). As the preliminary test, oral fluid samples were screened for amphetamines by Fluorescence Polarization Immunoassay (TDxFLx, Abbott Co.). Extraction for MA was performed using solid-phase extraction (SPE) by $RapidTrace^{TM}$ (Zymark, USA) with mixed mode cation exchange cartridge, CLEAN $SCREEN^{(R)}$ (130 mg/3 ml, UCT) after dilution with phosphate buffer. Samples were evaporated and derivatized by pentafluoropropionic acid anhydride (PFPA). Quantitation of MA and AM was performed by gas chromatography-mass spectrometry (GC-MS) using selective ion monitoring (SIM), the quantitation ions were m/z 204 (MA), 208 (MA-$D_5$), 190 (AM) and 194 (AM-$D_5$). The selectivity, linearity of calibration, limit of detection (LOD) and quantification (LOQ) within- and between day precision, accuracy and recoveries were examined as parts of the method validation. All oral fluid samples gave positive results to immunoassay for MA (cut-off level, 50 ng/ml as d-amphetamine). Concentrations of MA and AM by GC-MS in eleven samples were ranged 104.2${\sim}$4603.3 ng/ml and 32.4${\sim}$268.6 ng/ml, respectively. Extracted calibration curves of MA and AM were linear over the two concentration range of 1${\sim}$100 and 50${\sim}$1000 ng/ml with correlation coefficient of above 0.999. LOQ of MA and AM was 1 and 3 ng/ml, respectively. The intraand inter-day run precisions (CV) for MA and AM were less than 10%, and the accuracies (bias) for MA and AM were also less than 10% at the two different concentrations 5 and 100 ng/ml at low calibration range, 50 and 1000 ng/ml at high calibration range. The absolute recoveries of MA and AM at low and high calibration ranges were more than 82% and 75%, respectively. In this study the qualitative and quantitative analytical method of MA in oral fluid was established. Oral fluid testing may detect drug use in past hours because of its shorter detection window than urine, and be useful in post-accident situations. So oral fluids will be most useful for testing drug abuse in the driving under the influence of drug (DUID) as the alternative specimens of urine.

• 한국융합학회논문지
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• 제10권10호
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• pp.75-80
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• 2019

학교에서 의료용 초음파 실습 시 초음파 장치의 Freeze 및 Print Button, 프로브의 Lens, 손잡이, Line 부분 그리고 복부 Phantom에서 가장 접촉이 많은 부위를 임의로 선정하여 프로브에 상제하고 있는 세균을 검출하여 병원균의 수를 알아보고자 한다. 실험 방법으로는 실험 대상 위치에 균채집용 멸균된 면봉으로 검사 대상 부위를 20번 문지른 후 Lysogeny broth(LB) agar에 도말을 실시한 후 배양기에 넣어 48시간 동안 배양하고 colony forming unit (CFU) 수를 평가하여 프로브 손잡이, 복부 Phantom의 세균 분포정도를 알아보았다. 그 결과 CFU 값은 Lens는 $3.0{\pm}0.87$, Print button는 $5.5{\pm}1.06$, Freeze button은 $8.0{\pm}4.95$, Phantom은 $20.0{\pm}2.78$, Line은 $23.5{\pm}2.50$, 그리고 Probe handle의 값은 $35.3{\pm}10.75$로 측정되었다. 본 연구에서는 의료용 초음파 실습 시 실습 시 장비의 감염관리에 대한 주의를 부각시키고, 나아가서 초음파 장치의 세균 감염률 감소에 확실하게 기여 할 수 있을 것으로 기대된다.