• Journal of the Korean BIBLIA Society for library and Information Science
• /
• v.32 no.4
• /
• pp.213-229
• /
• 2021

The purpose of this study is to explore the establishment and administrative strategies for shared repositories for academic libraries in Korea, using the US ReCAP (Research Collections and Preservation Consortium) Project as a case study. The US ReCAP Project was a collaboration between the New York Public Library and the university libraries of Columbia University, Harvard University, and Princeton University. In-depth interviews were conducted with librarians and administrators in charge of the ReCAP Project to understand the original vision of the project, as well as how the strategy and approach evolved over the years. The analysis results revealed that shared repositories for libraries have evolved into shared collections enabling shared use. But shared repositories require a central management system to be successful in the long-term. Additionally, findings showed that the shared repository filled more quickly than anticipated. This in turn required collaborative planning on shared collection development and selection policies to determine which materials to send to the shared repository.

• Journal of Microbiology and Biotechnology
• /
• v.32 no.6
• /
• pp.749-760
• /
• 2022

α-Galactosidase is a debranching enzyme widely used in the food, feed, paper, and pharmaceuticals industries and plays an important role in hemicellulose degradation. Here, T26, an aerobic bacterial strain with thermostable α-galactosidase activity, was isolated from laboratory-preserved lignocellulolytic microbial consortium TMC7, and identified as Parageobacillus thermoglucosidasius. The α-galactosidase, called T26GAL and derived from the T26 culture supernatant, exhibited a maximum enzyme activity of 0.4976 IU/ml when cultured at 60℃ and 180 rpm for 2 days. Bioinformatics analysis revealed that the α-galactosidase T26GAL belongs to the GH36 family. Subsequently, the pET-26 vector was used for the heterologous expression of the T26 α-galactosidase gene in Escherichia coli BL21 (DE3). The optimum pH for α-galactosidase T26GAL was determined to be 8.0, while the optimum temperature was 60℃. In addition, T26GAL demonstrated a remarkable thermostability with more than 93% enzyme activity, even at a high temperature of 90℃. Furthermore, Ca²⁺ and Mg²⁺ promoted the activity of T26GAL while Zn²⁺ and Cu²⁺ inhibited it. The substrate specificity studies revealed that T26GAL efficiently degraded raffinose, stachyose, and guar gum, but not locust bean gum. This study thus facilitated the discovery of an effective heat-resistant α-galactosidase with potent industrial application. Meanwhile, as part of our research on lignocellulose degradation by a microbial consortium, the present work provides an important basis for encouraging further investigation into this enzyme complex.

• Journal of Animal Science and Technology
• /
• v.65 no.2
• /
• pp.387-400
• /
• 2023

Ruminal protozoa, especially entodiniomorphs, engulf other members of the rumen microbiome in large numbers; and they release oligopeptides and amino acids, which can be fermented to ammonia and volatile fatty acids (VFAs) by amino acid-fermenting bacteria (AAFB). Studies using defaunated (protozoa-free) sheep have demonstrated that ruminal protozoa considerably increase intraruminal nitrogen recycling but decrease nitrogen utilization efficiency in ruminants. However, direct interactions between ruminal protozoa and AAFB have not been demonstrated because of their inability to establish axenic cultures of any ruminal protozoan. Thus, this study was performed to evaluate the interaction between Entodinium caudatum, which is the most predominant rumen ciliate species, and an AAFB consortium in terms of feed degradation and ammonia production along with the microbial population shift of select bacterial species (Prevotella ruminicola, Clostridium aminophilum, and Peptostreptococcus anaerobius). From an Ent. caudatum culture that had been maintained by daily feeding and transfers every 3 or 4 days, the bacteria and methanogens loosely associated with Ent. caudatum cells were removed by filtration and washing. An AAFB consortium was established by repeated transfers and enrichment with casamino acids as the sole substrate. The cultures of Ent. caudatum alone (Ec) and AAFB alone (AAFB) and the co-culture of Ent. caudatum and AAFB (Ec + AAFB) were set up in three replicates and incubated at 39℃ for 72 h. The digestibility of dry matter (DM) and fiber (NDF), VFA profiles, ammonia concentrations, pH, and microscopic counts of Ent. caudatum were compared among the three cultures. The co-culture of AAFB and Ent. caudatum enhanced DM degradation, VFA production, and Ent. caudatum cell counts; conversely, it decreased acetate: propionate ratio although the total bacterial abundance was similar between Ec and the Ec + AAFB co-culture after 24 h incubation. The ammonia production and relative abundance of C. aminophilum and P. anaerobius did not differ between AAFB alone and the Ec + AAFB co-culture. Our results indicate that Ent. caudatum and AAFB could have a mutualistic interaction that benefited each other, but their interactions were complex and might not increase ammoniagenesis. Further research should examine how such interactions affect the population dynamics of AAFB.

• Journal of Korean Library and Information Science Society
• /
• v.55 no.1
• /
• pp.215-237
• /
• 2024

This study proposes a selection plan for research entities and PIDs and a strategy for building and operating a PID consortium based on a survey of advanced cases of research entities and PID operations in major countries such as the United Kingdom, Germany, Canada, Japan, China, and Australia. The criteria for selecting research entities and PIDs are 'research life cycle' and 'PID infrastructure maturity'. Based on the two selection criteria, it is proposed to prioritize research entity-PID pairs such as 'Researcher-ORCID', 'Publication-DOI', 'Data-DOI', 'Institution-ROR', 'Grant-DOI', and 'Project-RAiD' and expand to other research entities and PIDs in the emerging stage. The strategy for establishing and operating a PID consortium should encourage the participation of various PID stakeholders, identify the latest trends through collaborative networks with domestic and international PID organizations, lead education and outreach activities to raise awareness and increase utilization of PID, and secure policy support and financial stability. This is expected to lay the foundation for domestic research entities to gain visibility and accessibility at the global level.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.2
• /
• pp.219-227
• /
• 2018

The consortium for the HRD ability magnified program is part of the joint training program for SMEs in the strategic field and supports the infrastructure, training, personnel, and general operation expenses required for the joint training. The purpose of this study was to analyze the factors affecting the satisfaction of the education and training program, as perceived by the participants of the Consortium for the HRD Ability Magnified Program. The results of this study are as follows. Gender, trainer satisfaction, educational facilities and service satisfaction have a statistically significant effect on training program satisfaction. The impact on education and training satisfaction of gender, instructor satisfaction, educational (facilities) and service satisfaction was high and in that order. It was found that the male participants have a more positive effect on satisfaction of education and training than the female participants. Age, job group, and job title did not have a statistically significant effect on training program satisfaction. Based on the results of this study, the following suggestions are made. The capacity of the trainers should be strengthened. It is important to provide facilities that enable trainees to participate effectively in education and training and increase the transfer of education and training to the field. It is necessary to design and implement a curriculum that takes into consideration the female trainees' characteristics and situations. More effort is needed to improve the appropriateness of the education and training contents, education and training time, job relevance, and job contribution.

• Research in Plant Disease
• /
• v.13 no.3
• /
• pp.223-227
• /
• 2007

Lichen is a symbiotic association which lichen-forming fungi associate with green algae or cyanobacteria capable of producing food by photosynthesis. Lichen is one of the most widely distributed eukaryotic organisms on every kind of surface of soils, rocks and trees. Lichen-forming fungi commonly colonize on the surface of barks of dead or dying trees, which they use merely as a means of support without parasitizing. Lichen consortium of Parmotrema autrosinense and several crustose lichens heavily colonized on the surface of P. mume in Suncheon, Jeonnam province in Korea. The lichen consortium on the surface of P. mume gave the affected trees a dull gray appearance and suppressed their normal growths. Severely affected twigs or branches resulted in the death when the lichen thalli were not rubbed off the barks of P. mume. Twigs or branches of P. mume were recovered from blight syndrome when the lichen thalli were rubbed off, The recovered twigs or branches became healthy and clean when $Tween^{(R)}$ 20 or thiophanate-methyl paste were treated on the barks of P. mume. This is the first report of lichen diseases on P. mume caused by colonization of consortium of several crustose lichens including P. autrosinense.

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.441-444
• /
• 2002

This study was targeted to develope a microbial consortium having high cellulase production. A filamentous fungus, strain FB01, isolated from a compost showed high ${\beta}-glucosidase$ activity especially. The strain FBOl was co-cultured with Trichoderma viride to enhance the productivity of ${\beta}-glucosidase$, changing inoculation time of one strain (FB01). The microbial consortium prepared showed the higher cellulytic enzyme production than T. viride well-known. The maximal enzyme production was obtained when the microbial consortium was cultured at $30^{\circ}C$ and pH 6.0 for 10days and the activities of CMCase, ${\beta}-glucosidase$, and avicelase were 2.0, 0.8, and 0.2 U/mL, respectively. These enzyme activities were 2, 4, and 2 times as high as those of CMCase, ${\beta}-glucosidase$, avicelase from T. viride, respectively, indicating that a synergistic interaction appeared between T viride and strain FB01. The serial subcultures by pH control increased ${\beta}-glucosidase$ production about 3.2 times. Also, enzyme production using rice-straw as a carbon source showed that the activities of CMCase, ${\beta}-glucosidase$, and avicelase were 3.69, 0.76, 0.17 U/mL, respectively, and ${\beta}-glucosidase$ activity was 1.5 times higher than that of T. viride. Consequently, microbial consortium showed the considerabely enhanced production of the cellullolytic enzymes, such as CMCase, ${\beta}-glucosidase$, and avicelase compared those of T. viride, and a favorable stability for the enzyme production even in the serial subcultures.

• KSBB Journal
• /
• v.17 no.4
• /
• pp.381-387
• /
• 2002

A filamentous fungus, strain FB01 showing high $\beta$-glucosidase activity was isolated from a compost. This fungus was cocultured with Trichoderma viride to enhance the productivity of $\beta$-glucosidase by changing inoculation time of the fungus. The microbial consortium showed higher cellulolytic enzyme production than T. viride alone. The maximal enzyme production was obtained when the microbial consortium was cultured at 30$\^{C}$ and pH 6.0 for 10 days with the activities of CMCase, $\beta$-glucosidase, and avicelase of 2.0, 0.8, and 0.2 U/mL, respectively. These enzyme activities were 2, 4, and 2 times as high as those of CMCase, p-glucosidase, avicelase from T. viride, respectively, indicating that a synergistic interaction appeared between T. viride and strain FBOI . The serial subcultures with pH control increased $\beta$-glucosidase production about 3.2 times. Enzyme production using ricestraw as a carbon source showed that the activities of CMCase, $\beta$-glucosidase, and avicelase were 3.69, 0.76, 0.17 U/mL, respectively, and $\beta$-glucosidase activity was 1.5 times higher than that of T viride.

• Journal of Ginseng Research
• /
• v.40 no.1
• /
• pp.18-27
• /
• 2016

Background: It is not clear whether ginseng affects cyclosporine A (CsA)-induced desirable immunosuppressive action. In this study, we evaluated the immunological influence of combined treatment of ginseng with CsA. Methods: Using CD4+ T cells from mouse spleens stimulated with the T cell receptor (TCR) or allogeneic antigen-presenting cells (APCs), we examined the differentiation of naïve T cells into T helper 1 (Th1), Th2, Th17, and regulatory T cells (Tregs), and their cytokine production during treatment by Korean Red Ginseng extract (KRGE) and/or CsA. The influence of KRGE on the allogeneic T cell response was evaluated by mixed lymphocyte reaction (MLR). We also evaluated whether signal transducer and activator of transcription 3 (STAT3) and STAT5 are implicated in this regulation. Results: Under TCR stimulation, KRGE treatment did not affect the population of CD4+interferon gamma ($IFN{\gamma}$)+ and CD4+interleukin (IL)-4+ cells and their cytokine production compared with CsA alone. Under the Th17-polarizing condition, KRGE significantly reduced the number of CD4+IL-17+ cells and CD4+/phosphorylated STAT3 (p-STAT3)+ cells, but increased the number of CD4+CD25+forkhead box P3 (Foxp3)+ cells and CD4+/p-STAT5+ cells compared with CsA alone. In allogeneic APCs-stimulated CD4+ T cells, KRGE significantly decreased total allogeneic T cell proliferation. Consistent with the effects of TCR stimulation, KRGE reduced the number of CD4+IL-17+ cells and increased the number of CD4+CD25+Foxp3+ cells under the Th17-polarizing condition. Conclusion: KRGE has immunological benefits through the reciprocal regulation of Th17 and Treg cells during CsA-induced immunosuppression.

• Journal of Korean Library and Information Science Society
• /
• v.50 no.2
• /
• pp.121-147
• /
• 2019

The purpose of this study is to investigate the perceptions of the organizations in the fields of academics, arts, humanities, and music in Korea on the International Standard Name Identifier (ISNI) which is developed by International Standard Organization (ISO) for assuring the public identities of creators, contributors, and producers to contents in the fields. National Library of Korea (NLK), one of the official registration agencies of ISNI in Korea, launched a consortium, ISNI-Korea, with various organizations in the fields to promote the ISNI registration and application. In this study, focused group interviews were carried out with participants from a total of 13 organizations; these organizations were divided into three groups by the progress on collaboration with NLK at the ISNI-Korea consortium. The current status of the personnel or membership information management by organizations, their expectations and barriers for using ISNI, and the ISNI application to the fields have been discussed intensively during the interviews. Findings from this study could benefit NLK to develop the strategies to promote ISNI in the fields. This study also proposed follow-up studies to enhance the use of ISNI in Korea.