• 한국정보처리학회논문지
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• 제4권6호
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• pp.1589-1600
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• 1997

광케이블에 의한 가입자 엑세스 망의 구성은 투자비면에서 볼 때 40~50% 이상의 많은 비용이 소요되는 부분으로 이 분야의 최적화는 광대역 종합정보통신망 구축의 핵심 분야이다. 따라서 선진 각국에서는 이에 대한 연구를 활발히 진행하고 있다. 그러므로 본 논문에서는 가장 보편적으로 이용될 수 있는 이중 성형 구조의 광가입자 엑세스 망 구조 중 HFC, ATM-PON/FTTC 그리고 Super PON 구조를 가지고 경제성 분석을 하였다. 경제성 분석을 위한 비교인자로는 투자비, 유지 보수비, 내용 년수 및 잔존가 등 광가입자 엑세스 망을 구축하기 위해 소요되는 여러가지 장비들을 선택하여 정적 비교 분석법을 TITAN 모델에 적용시켜 소요비용을 산출한 결과 ATM-PON/FTTC 구조가 가장 경제적인 구조로 판명되었다. 이것은 통신망 사업자의 가입자망 구조 결정시에 가이드 라인이 될 수 있다.

• Mycobiology
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• 제44권3호
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• pp.155-161
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• 2016

The most economically important species used in a wide range of fermentation industries throughout Asia belong to Aspergillus section Flavi, which are morphologically and phylogenetically indistinguishable, with a few being toxigenic and therefore a major concern. They are frequently isolated from Korean fermentation starters, such as nuruk and meju. The growing popularity of traditional Korean alcoholic beverages has led to a demand for their quality enhancement, therefore requiring selection of efficient non-toxigenic strains to assist effective fermentation. This study was performed to classify the most efficient strains of Aspergillus section Flavi isolated from various types of traditional wheat nuruk, based on a polyphasic approach involving molecular and biochemical evaluation. A total of 69 strains were isolated based on colony morphology and identified as Aspergillus oryzae/flavus based on internal transcribed spacer and calmodulin gene sequencing. Interestingly, none were toxigenic based on PCR amplification of intergenic regions of the aflatoxin cluster genes norB-cypA and the absence of aflatoxin in the culture supernatants by thin-layer chromatography analysis. Saccharification capability of the isolates, assessed through ${\alpha}-amylase$ and glucoamylase activities, revealed that two isolates, TNA24 and TNA15, showed the highest levels of activity. Although the degrees of variation in ${\alpha}-amylase$ and glucoamylase activities among the isolates were higher, there were only slight differences in acid protease activity among the isolates with two, TNA28 and TNA36, showing the highest activities. Furthermore, statistical analyses showed that ${\alpha}-amylase$ activity was positively correlated with glucoamylase activity (p < 0.001), and therefore screening for either was sufficient to predict the saccharifying capacity of the Aspergillus strain.

• 한국연초학회지
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• 제28권2호
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• pp.100-110
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• 2006

A mutant of Potato vims Y (PVY) was occurred in PVY resistance flue-cured tobacco breeding line KF0402 $(TC1146{\times}KF117)$ showing vein necrosis at Suwon in Korea. This isolate, PVY-SWM, was differentiated from other PVY based on biological properties and nucleotide sequence analyses of coat protein gene. PVY-SWM caused typical symptoms on 21 indicator plants as compared to the PVY-TOJC37. Remarkably, the PVY-SWM induced distinctly different symptom of systemic vein necrosis on tobacco cultivars V.SCR, PBD6, TN86, TN90, Virgin A Mutant (VAM), Wislica, NC744, KB108 and KB111, which were reported to have the recessive potyvirus resistance gene va. In RT-PCR assays with specific primers for detection of PVY, a single band of about 800bp in length was produced. The amplified DNA was cloned and the nucleotide sequence was determined. The coat protein gene of PVY-SWM showed 88.4%-99.0% and 92.5%-98.5% identities to the 12 different PVY isolates of Genbank Database at the nucleotide and amino acidi respectively. Multiple alignments as well as cluster dendrograms of PVY-SWM isolate revealed close phylogenetic relationship to the $PVY^{NTN}$ subgroup.

• Human Ecology Research
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• 제53권2호
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• pp.143-155
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• 2015

This study analyzed domestic consumers' perceptions of and participation in consumer boycotts and its relation to ethical consumerism by surveying 1,000 people aged 20 or more with an on-line questionnaire. Collected data were analyzed in terms of frequency, factor analyses, cluster analysis, ${\chi}^2$, and one-way analysis of variance. The research findings are summarized as follows. First, consumers had an average (or higher) perception level of consumer boycotts and were categorized into consumer boycott groups of pursuit of self-realization, achievement of results, and pursuit of rationality according to perception patterns of consumer boycotts. Second, the consumer boycott group of self-realization exhibited a high intention for participation in economic and ethical consumer boycotts and was active in all aspects compared to other groups; in addition, the consumer boycott group of results achievement was in the lowest level of ethical consumerism with a low intention for participation in consumer boycotts and ethical consumer boycotts along with the lowest perception level for consumer boycotts as ethical consumption. The consumer boycott group of rational pursuit was comparatively skeptical about the influence of consumer boycotts and with a low level of deontological ethical consumerism. They had additional doubts about the effectiveness of consumer boycotts despite being active participants. The study contributes to the establishment of an ethical consumer behavior theory by investigating connections between the perception patterns of and participation in consumer boycotts as well as ethical consumerism as consumer boycotts conceptually expand as a form of ethical consumption.

• 전기화학회지
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• 제15권1호
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• pp.35-40
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• 2012

The composite membranes using Nafion as matrix and 1-ethyl-3-methylimidazolium tetracyanoborate (EMITCB) as ion-conducting medium in replacement of water were prepared and characterized. The amount of EMITCB in Nafion varied from 30 to 50wt%. The composite membranes are characterized by ion conductivity, thermogravitational analyses (TGA) and small-angle X-ray scattering (SAXS). The composite membranes containing EMITCB of 40wt% showed the maximum ionic conductivity which was ~0.0146 S $cm^{-1}$ at 423.15 K. It is inferred that the decrease in ionic conductivity of all the composite membranes might be due to the decomposition of a tetracyanoboric acid formed in the composite membranes. The results of SAXS indicated that the ionic clusters to conduct proton in the composite membranes were successfully formed. In accordance with the results of ionic conductivity as a function of a reciprocal temperature, SAXS showed a proportional decrease in scattering maximum $q_{max}$ as the amount of EMITCB increases in the composite membranes, which results in the increase in ionomer cluster size. The TGA showed no significant decomposition of the ionic liquid as well as the composite membranes in the range of operating temperature ($120-150^{\circ}C$) of high temperature proton exchange membrane fuel cells (HTPEMFC). As a result, EMITCB is able to play an important role in transferring proton in the composite membranes at elevated temperatures with no external humidification for proton exchange membrane fuel cells.

• BMB Reports
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• 제38권5호
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• pp.595-601
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• 2005

In this study, we have isolated a rice (Oryza sativa L.) glutamate decarboxylase (RicGAD) clone from a root cDNA library, using a partial Arabidopsis thaliana GAD gene as a probe. The rice root cDNA library was constructed with mRNA, which had been derived from the roots of rice seedlings subjected to phosphorus deprivation. Nucleotide sequence analysis indicated that the RicGAD clone was 1,712 bp long, and harbors a complete open reading frame of 505 amino acids. The 505 amino acid sequence deduced from this RicGAD clone exhibited 67.7% and 61.9% identity with OsGAD1 (AB056060) and OsGAD2 (AB056061) in the database, respectively. The 505 amino acid sequence also exhibited 62.9, 64.1, and 64.2% identity to Arabidopsis GAD (U9937), Nicotiana tabacum GAD (AF020425), and Petunia hybrida GAD (L16797), respectively. The RicGAD was found to possess a highly conserved tryptophan residue, but lacks the lysine cluster at the C-proximal position, as well as other stretches of positively charged residues. The GAD sequence was expressed heterologously using the high copy number plasmid, pVUCH. Our activation analysis revealed that the maximal activation of the RicGAD occurred in the presence of both $Ca^{2+}$ and calmodulin. The GAD-encoded 56~58 kDa protein was identified via Western blot analysis, using an anti-GAD monoclonal antibody. The results of our RT-PCR analyses revealed that RicGAD is expressed predominantly in rice roots obtained from rice seedlings grown under phosphorus deprivation conditions, and in non-germinated brown rice, which is known to have a limited phosphorus bioavailability. These results indicate that RicGAD is a $Ca^{2+}$/calmodulin-dependent enzyme, and that RicGAD is expressed primarily under phosphate deprivation conditions.

• 복식
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• 제56권3호
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• pp.143-155
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• 2006

This study aims to examine the difference of body image and psychological well-being of men and women according to appearance management. The subjects of the study were 673 college students who lived in Seoul and metropolitan areas. Statistical analyses used in the study were factor analysis, Cronbach's a, cluster analysis, ANOVA, Duncan test and $\chi^2-test$. The results of this study were as follows; 1. The body image was categorized into the three factors of personal interest innce, anxiety about weight and physical attraction. Appearance management was categorized into the appearance management through clothing, strict diet therapy and daily appearance management. The psychological well-being was classified as seven factors which include the life goal, self-acceptance, positive personal relations, self-regulation, personal development, environment control and responsibility. 2. The appearance management was classified by 4 groups as below: Daily appearance management group, strict diet therapy group, appearance management through clothing group and no interest in appearance group. 3. There were significant differences among the four groups regarding body image, psychological well-being and life satisfaction. First, appearance management through clothing group had more interest in appearance than other groups. In the case of weight awareness, strict diet therapy group and the appearance management through clothing group intended to have more concerns over their weight. The strict diet therapy group seemed to have an overall lower level of psychological well-being than other groups. Lastly, the appearance management through clothing group had a higher level of life satisfaction.

• BMB Reports
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• 제53권8호
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• pp.425-430
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• 2020

Tumor necrosis factor-inducible gene 6 protein (TSG-6) is a cytokine secreted by mesenchymal stem cells (MSCs) and regulates MSC stemness. We previously reported that TSG-6 changes primary human hepatic stellate cells (pHSCs) into stem-like cells by activating yes-associated protein-1 (YAP-1). However, the molecular mechanism behind the reprogramming action of TSG-6 in pHSCs remains unknown. Cluster of differentiation 44 (CD44) is a transmembrane protein that has multiple functions depending on the ligand it is binding, and it is involved in various signaling pathways, including the Wnt/β-catenin pathway. Given that β-catenin influences stemness and acts downstream of CD44, we hypothesized that TSG-6 interacts with the CD44 receptor and stimulates β-catenin to activate YAP-1 during TSG-6-mediated transdifferentiation of HSCs. Immunoprecipitation assays showed the interaction of TSG-6 with CD44, and immunofluorescence staining analyses revealed the colocalization of TSG-6 and CD44 at the plasma membrane of TSG-6-treated pHSCs. In addition, TSG-6 treatment upregulated the inactive form of phosphorylated glycogen synthase kinase (GSK)-3β, which is a negative regulator of β-catenin, and promoted nuclear accumulation of active/nonphosphorylated β-catenin, eventually leading to the activation of YAP-1. However, CD44 suppression in pHSCs following CD44 siRNA treatment blocked the activation of β-catenin and YAP-1, which inhibited the transition of TSG-6-treated HSCs into stem-like cells. Therefore, these findings demonstrate that TSG-6 interacts with CD44 and activates β-catenin and YAP-1 during the conversion of TSG-6-treated pHSCs into stem-like cells, suggesting that this novel pathway is an effective therapeutic target for controlling liver disease.

• 한국콘텐츠학회논문지
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• 제14권10호
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• pp.87-100
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• 2014

본 연구의 목적은 시청자들의 방송심의에 대한 인식유형을 도출하고 그 특성을 분석하는 동시에, 시청자들에게 나타나는 인식 반응의 비일관성을 체계적으로 파악하는 것이다. 주요 연구결과는 다음과 같다. 첫째, 설문조사 자료를 이용하여 요인분석과 군집분석 등 통계적 방법으로 방송심의 활동에 대한 시청자들의 인식유형을 구분하여 그 특성을 분석하였다. 시청집단별 인식유형은 '엄격기준 적극심의 선호형' 등 6개 유형으로 분류하였다. 6개 유형 가운데 3개 유형이 적극적인 심의를 선호하는 유형으로 나타나고 있다. 이중 '최저기준 적극심의 선호형' 집단은 심의기준 항목의 중요성을 가장 낮게 평가하면서도, 심의활동은 강화되어야 한다는 논리적으로 모순된 인식과 반응을 보이고 있다. 시사점은 방송심의 활동에 대한 시청자의 인식 유형을 통계적 방법을 활용하여 체계적으로 분류하고, 그 특성을 구체적으로 분석, 향후 심의에 대한 시청자들의 인식과 요구를 보다 과학적으로 파악할 수 있는 기초를 마련한 것이라고 볼 수 있다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.82-82
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• 2017

The production of spring wheat, the major crop in Mongolia, is accounting for 98% of the cultivated area. Collection, conservation and utilization of wheat germplasm resources play an important role in wheat breeding and production in Mongolia. Understanding genetic variability in the existing genebank accessions is important for collection and conservation of wheat germplasms. To determine the genetic diversity and population structure among a representative collection of Mongolian local wheat cultivars and lines, 200 wheat accessions were analyzed with 15 SSR markers distributed throughout the wheat genome. A total of 85 alleles were detected, with 3 to 5 alleles per locus and a mean genetic diversity value of 5.66. The average genetic diversity index was 0.68, with values ranging from 0.37 to 0.80. The 200 Mongolian wheat accessions were divided into two subgroups based on STRUCTURE, un-rooted NJ cluster and principal coordinate analyses. The results from this study will provide important information for future wheat germplasm conservation and improvement programs with Mongolian genebank.