Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
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Characteristics of Potato Virus Y (PVY) Mutant Isolated from PVY Resistance Breeding Line in Korea

국내 감자바이러스 Y (PVY) 저항성 육성 계통에서 분리한 PVY Mutant의 특성

  • Kim, Jae-Hyun (Bio-resources Research Group, KT&G Central Research Institute) ;
  • Kuem, Wan-Soo (Bio-resources Research Group, KT&G Central Research Institute) ;
  • Lee, Sin-Ho (Dept. of Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jeong-Soo (Dept. of Horticultural Research Institute, Rural Development Administration) ;
  • Jeon, Yong-Ho (Bio-resources Research Group, KT&G Central Research Institute) ;
  • Jung, Suk-Hun (Bio-resources Research Group, KT&G Central Research Institute) ;
  • Chung, Youl-Young (Bio-resources Research Group, KT&G Central Research Institute) ;
  • Park, Yong-Hack (Bio-resources Research Group, KT&G Central Research Institute)
  • 김재현 (KT&G 중앙연구원 생물자원연구소) ;
  • 금완수 (KT&G 중앙연구원 생물자원연구소) ;
  • 이신호 (농촌진흥청 원예연구소) ;
  • 김정수 (농촌진흥청 원예연구소) ;
  • 전용호 (KT&G 중앙연구원 생물자원연구소) ;
  • 정석훈 (KT&G 중앙연구원 생물자원연구소) ;
  • 정열영 (KT&G 중앙연구원 생물자원연구소) ;
  • 박용학 (KT&G 중앙연구원 생물자원연구소)
  • Published : 2006.12.31
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Abstract

A mutant of Potato vims Y (PVY) was occurred in PVY resistance flue-cured tobacco breeding line KF0402 $(TC1146{\times}KF117)$ showing vein necrosis at Suwon in Korea. This isolate, PVY-SWM, was differentiated from other PVY based on biological properties and nucleotide sequence analyses of coat protein gene. PVY-SWM caused typical symptoms on 21 indicator plants as compared to the PVY-TOJC37. Remarkably, the PVY-SWM induced distinctly different symptom of systemic vein necrosis on tobacco cultivars V.SCR, PBD6, TN86, TN90, Virgin A Mutant (VAM), Wislica, NC744, KB108 and KB111, which were reported to have the recessive potyvirus resistance gene va. In RT-PCR assays with specific primers for detection of PVY, a single band of about 800bp in length was produced. The amplified DNA was cloned and the nucleotide sequence was determined. The coat protein gene of PVY-SWM showed 88.4%-99.0% and 92.5%-98.5% identities to the 12 different PVY isolates of Genbank Database at the nucleotide and amino acidi respectively. Multiple alignments as well as cluster dendrograms of PVY-SWM isolate revealed close phylogenetic relationship to the $PVY^{NTN}$ subgroup.

Keywords

References

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