• The Journal of Natural Sciences
• /
• v.15 no.1
• /
• pp.79-88
• /
• 2005

There are five isoforms of thiol peroxidase in yeast. Each isoform was named after its subcellular localization such as cytoplasmic TPx I, cTPx II, cTPx III, mitochondrial TPx (mTPx), and nuclear TPx (nTPx). Recently, we reported that unlike other TPx null mutants, cTPx IInull mutant showed a slow-growth phenotype. This observation suggests that cTPx II might be involved in yeast cell growth. In this study, for a first step toward to investigate the physiological function of cTPx II in yeast, we have identified a novel interaction between cTPx II and various proteins by using the yeast two-hybrid system.

• Korean Journal of Environmental Biology
• /
• v.38 no.2
• /
• pp.289-298
• /
• 2020

As part of the research program "2018 Rapid screening and identification of freshwater microorganisms using MALDI-TOF/MS library" freshwater samples were collected from a branch of the Nakdong River. Almost 300 antibiotic-resistant bacterial strains were isolated from freshwater samples and subsequently identified by 16S rRNA gene sequencing. Seventeen strains among the isolates shared high 16S rRNA gene sequence similarity (>99.0%) with known species that were not previously recorded in Korea, and each of the isolates also formed a robust phylogenetic clade with the closest species. These species were phylogenetically diverse, belonging to four phyla, seven classes, 10 orders, and 13 genera. At the genus and class level, the previously unrecorded species belonged to Rhodovarius, Xanthobacter, and Shinella of the class Alphaproteobacteria; Ottowia, Simplicispira, and Zoogloea of Betaproteobacteria; Pseudomonas, Acinetobacter, and Shewanella of Gammaproteobacteria; Arcobacter of Epsilonproteobacteria; Sphingobacterium of Sphingobacteriia; Trichococcus of Bacilli; and Leucobacter of Actinobacteria. The previously unrecorded species were further characterized by examining their gram-staining, colony and cell morphology, biochemical properties, and phylogenetic position.

• Proceedings of the Korea Society of Poultry Science Conference
• /
• 1999.11a
• /
• pp.34-50
• /
• 1999

A cDNA encoding chicken interferon-gamma (chIFN-${\gamma}$) was amplified from P34, a CD4$^{+}$ T-cell hybridoma by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into pUC18. THe sequences of cloned PCR products were determined to confirm the correct cloning. Using this cDNA as probe, chicken genomic library from White Leghorn spleen was screened. Phage clones harboring chicken interferon-gamma (chIFN-${\gamma}$) were isolated and their genomic structure elucidated. The chIFN-${\gamma}$ contains 4 exons and 3 introns spanning over 14 kb, and follows the GT/AG rule for correct splicing at the exon/intron boundaries. The four exons encode 41, 26, 57 and 40 amino acids, respectively, suggesting that the overall structure of IFN-${\gamma}$ is evolutionairly conserved in mammalian and avian species. The 5’-untranslated region and signal sequences are located in exon 1. Several AT-rich sequences located in the fourth exon may indicate a role in mRNA turnover. The 5’-flanking region contains sequences homologous to the potential binding sites for the mammalian transcription factors, activator protein-1(AP-1) activator protein-2(AP-2) cAMP-response element binding protein(CREB), activating transcription factor(ATF), GATA-binding fator(GATA), upstream stimulating factor(USF), This suggests that the mechanisms underlying transcriptional regulation of chicken and mammalian IFN-${\gamma}$ genes may be similar.r.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.17 no.5
• /
• pp.193-197
• /
• 2017

In this paper, we present a low-power delta-sigma based digital frequency synthesizer with high frequency resolution for bio sensor networks. Biomedical radio-frequency (RF) transceivers require miniaturized forms with a long battery life and low power consumption. For the technology scaling, digital circuits have become preferable compared to analog circuits because of the aggressive cost, size, flexibility, and repeatability. Therefore, the digital circuits based on standard-cell library are used to reduce a power consumption. Additionally, a delta-sigma is used for making fractional frequency tuning range. From the simulation, we confirmed that proposed scheme has good performance in accordance with power and frequency resolution.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.15 no.2
• /
• pp.255-266
• /
• 2015

In this paper, a low-complexity and low-power soft output multiple input multiple output (MIMO) symbol detector is proposed for mobile devices with two transmit and two receive antennas. The proposed symbol detector can support both the spatial multiplexing mode and spatial diversity mode in single hardware and shows the optimal maximum likelihood (ML) performance. By applying a multi-stage pipeline structure and using a complex multiplier based on the polar-coordinate, the complexity of the proposed architecture is dramatically decreased. Also, by applying a clock-gating scheme to the internal modules for MIMO modes, the power consumption is also reduced. The proposed symbol detector was designed using a hardware description language (HDL) and implemented using a 65nm CMOS standard cell library. With the proposed architecture, the proposed MIMO detector takes up an area of approximately $0.31mm^2$ with 183K equivalent gates and achieves a 150Mbps throughput. Also, the power estimation results show that the proposed MIMO detector can reduce the power consumption by a maximum of 85% for the various test cases.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2005.05a
• /
• pp.1071-1074
• /
• 2005

The ECC(Elliptic Curve Cryptogrphics), one of the representative Public Key encryption algorithms, is used in Digital Signature, Encryption, Decryption and Key exchange etc. The key operation of an Elliptic curve cryptosystem is a scalar multiplication, hence the design of a scalar multiplier is the core of this paper. Although an Integer operation is computed in infinite field, the scalar multiplication is computed in finite field through adding points on Elliptic curve. In this paper, we implemented scalar multiplier in Elliptic curve based on the finite field $GF(2^{163})$. And we verified it on the Embedded digital system using Xilinx FPGA connected to an EISC MCU(Agent 2000). If my design is made as a chip, the performance of scalar multiplier applied to Samsung $0.35\;{\mu}m$ Phantom Cell Library is expected to process at the rate of 8kbps and satisfy to make up an encryption processor for the Embedded digital information home system.

• Molecules and Cells
• /
• v.39 no.9
• /
• pp.687-691
• /
• 2016

Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous recombination in bacteria. Although the methods for TALE assembly are constantly improving, these methods are not convenient because of laborious assembly steps or large module libraries, limiting their broad utility. To overcome the barrier of multiple assembly steps, we report a one-step system for the convenient and flexible assembly of a 180 TALE module library. This study is the first demonstration to ligate 9 mono-/dimer modules and one circular TALEN backbone vector in a one step process, generating 9.5 to 18.5 repeat sequences with an overall assembly rate higher than 50%. This system makes TALEN assembly much simpler than the conventional cloning of two DNA fragments because this strategy combines digestion and ligation into one step using circular vectors and different modules to avoid gel extraction. Therefore, this system provides a convenient tool for the application of TALEN-mediated genome editing in scientific studies and clinical trials.

• Microbiology and Biotechnology Letters
• /
• v.38 no.1
• /
• pp.70-76
• /
• 2010

The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13 cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarray and antisense libraries for an effective functional validation of genes.

• Journal of the Korean Solar Energy Society
• /
• v.28 no.3
• /
• pp.15-20
• /
• 2008

Building-integrated photovoltaics(BIPV) are increasingly incorporated into new domestic and industrial buildings as a principal or ancillary source of electrical power, and are one of the fastest growing segments of the photovoltaic industry. This paper presents design, operational features analysis, and PCS(Power Conditioning System) of grid-connected 30kW BIPV set up on the library of Dongshin University. For a sustainable photovoltaics system in this area, the data of the BIPV system are collected and analyzed by monitoring system using LabView. PCS of the grid-connected BIPV system, also, is designed for optimal operation with characteristics suggested in this paper.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.3
• /
• pp.329-334
• /
• 2013

Uridinediphospho-N-acetylglucosamine enolpyruvyl transferase (MurA, E.C. 2.5.1.7) is an essential bacterial enzyme that catalyzes the first step of the cell wall biosynthetic pathway, which involves the transfer of an enolpyruvyl group from phosphoenolpyruvate to uridinediphospho-Nacetylglucosamine. In this study, novel inhibitors of Haemophilus influenzae MurA (Hi MurA) were identified using high-throughput screening of a chemical library from the Korea Chemical Bank. The identified compounds contain a quinoline moiety and have much lower effective inhibitory concentrations ($IC_{50}$) than fosfomycin, a wellknown inhibitor of MurA. These inhibitors appear to covalently modify the sulfhydryl group of the active site cysteine (C117), since the C117D mutant Hi MurA was not inhibited by these compounds and excess dithiothreitol abolished their inhibitory activities. The increased mass value of Hi MurA after treatment with the identified inhibitor further confirmed that the active-site cysteine residue of Hi MurA is covalently modified by the inhibitor.