• KSBB Journal
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• 제18권1호
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• pp.70-73
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• 2003

TLC를 이용하여 시료에 있는 젖산을 간편하고 신속하게 분리하고 정량 분석 할 수 있는 조건을 개발하였다. Silicagel TLC plate를 사용하는 경우 전개용매로 Nitromethane : 1-Propanol $H_2O$를 2 : 5 : 1.5 (v/v/v)의 비율로 섞어 사용하고 발색시약으로 Bromocresol purple 을 50% 에탄올 100 mL에 녹여(pH 10) 준비한 Bromocresol purple reagent를 사용하였을 때, 젖산은 엷은 주홍색의 TLC plate의 바탕에 자은 노란색 spot으로 확인되었으며 이 조건으로 0.5 - 4% 농도 범위의 젖산을 포함한 시료를 희석 없이 99.4%의 신뢰도로 쉽게 정량 분석할 수 있었고, 개발한 조건의 유효성은 HPLC 와 lactate dehydrogenase를 이용한 젖산 정량 분석으로 확인되었다.

• 인간식물환경학회지
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• 제23권5호
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• pp.521-535
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• 2020

Background and objective: This study investigated plant type and color preference and conducted a psychological assessment for gardening activities of firefighters in a high-risk occupational group. Methods: A survey was conducted on 117 firefighters aged 39.01±9.17 in Sunchang in March 2020 to examine their preferences in plant type and color and conduct a psychological assessment of firefighters on gardening activities. Results: For plant experience and awareness, plant-related event showed the highest score, followed by plant preference tendency and plant growing experience. The level of plant gardening activities was higher among older age and higher job position. As a result of examining the quality of life according to the demographic characteristic with WHOQOL-BREF (World Health Organization Quality of Life Assessment Instrument), it was found that the quality of life was higher among the 20s-30s than 50s, firefighter position than fire lieutenant or higher, those with higher average monthly income, and the singles group than married (p < .05). The administration group showed lower scores than the fire suppression group working in the field, showing statistical significance. Most firefighters showed high preference for herbal plants among the 12 types of flowering plants, in the order of Rosmarinus officinalis>Lavandula spp.>Mentha piperita, Calendula officinalis. Pelargonium spp. was preferred the least out of the flowering plants, Lycopersicon esculentum was preferred the most out of 12 types of vegetable plants, while Angelica acutiloba was preferred the least statistical significance. For the preference of plant colors, yellow was the most preferred color, followed by white, blue, orange, red, and green, showing statistical significance. For the psychological survey responses of emotion words toward 6 plants colors, it was found that 'bright' was the emotion felt most strongly by the white, yellow, and orange groups, 'calm' and 'comfortable' by the green and blue groups, and 'fancy' by the red group. Conclusion: The effect of agro-healing programs can be increased by using mostly yellow plants when creating indoor healing gardens at the workplaces of fire officials in a high-risk occupational group, and using herbal plants and Lycopersicon esculentum in the gardens for agro-healing activities.

• 복식
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• 제59권3호
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• pp.109-130
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• 2009

This study examines the rotor characteristics of Guybang culture (women's living in Joseon) relics which the life images and mental world of women in Joseon if expressed to prepare rotor data to be utilized in modern design and information for color identity establishment of Korean women culture. The result of this study is as following: 1. From 176 pieces of relic photos, total of 1380 color chips were extracted. For color analysis, 1368 units (99.1%) of color data excluding achromatic 12 units (0.9%) among 1380 units of colors were used. With the standard of 10 color wheel of Munsell Color System, the result of studying distributions according to frequency, red was the highest by 25.9%, yellow was 21.4% in women's goods in Joseon period which the ratio of red and yellow was 473%. Other than that, GY 14.6%, YR 11.3% and RP 11.1% followed next which is studied that the warm colors that conveys enjoyable and glamorous feelings composed 84.3%; 2. As a result of tone analysis, high value low chroma ltg color showed highest distribution of 20% and d was 12.2%, b was 11.7% and sf was 8.8%. Generally, it showed high frequency in high value cocors such as ltg, sf and b of 40.5%t and in low chroma (ltg) and medium chroma (d, sf) of 41% and colors in bright and calm image was used the most. 3. As a result of analyzing relationship between hue & tone, the ltg tone of Y close to white in traditional color sensibility showed the highest frequency of 9.6% and ltg tone of GY(6.4%), b tone of RP(6.1%), and v(5.4%), b(5.4%), dp(5.3%) of R followed next. 4. As a result of analyzing the characteristics of coloring, it showed that in case of coloring contrasting hues, it is adjusted similar tones and in case of coloring less contrasting hue, the difference in tone is adjusted to be rather large to make harmonized coloring. Moreover, in case of coloring similar hues, the ratio of using same tone and similar tone was large which the coloring of calm image and dynamic image is all used.

• 대한예방한의학회지
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• 제5권1호
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• pp.116-133
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• 2001

Antifibrotic drugs could be delayed or inhibited massive collagen deposition in liver tissue or inhibited collagen synthesis. we investigated antifibrotic effects by several herbs(Schisnadra chinensis, Ganoderma japonicum, Sedum sarmentosum, Alisma canaliculatum, Plantago asiatica) extract with observations of collagen accumulation in liver tissue and collagen synthesis in sera. Rats were used for experimental animal that were devided 3 groups(sham control, BDL/S, BDL/S-ER). Rats were operated for inducing liver fibrosis(cirrhosis) by bile duct obstruction. Several herbs were prepared by water extraction and were applicated p. o. $3ml/day$ during 4 weeks. After observation period, rats were sacrificed and liver tissue and sera were collected. In result, the mortality of rats was 35% in BDL/S group and 20% in BDL/S-ER The color of bile juice in BDL/S-ER was bright yellow and murky yellow in BDL/S group. The significantly lower weight of liver($16.21g{\pm}5.3,\;20.58{\pm}2.4$) and spleen($1.96g{\pm}0.96,\;3.93{\pm}0.21$) were shown in BDL/S-ER than that of BDL/S(p<0.05) group. The value of collagen in liver tissue(25.7%) in BDL/S-ER was observed significantly lower than that of BDL/S group (Tab. 2). AST. ALT, ALP, t-bilirubin, BUN levels were low in BDL/S-ER as compared with those of BDL/S group, but the significance was not proven. The trichrome stained liver tissue in BDL/S-ER group was observed mild bile duct proliferation and fibrosis compared with BDL/S group. In conclusion, natural products inhibited new collagen synthesis and delayed massive collagen deposition in liver tissue, so that they have noticeable antifibrotic effects in experimental liver fibrosis(cirrhosis).

• Plant Biotechnology Reports
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• 제5권2호
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• pp.187-195
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• 2011

The gesneriaceous perennial plant Titanotrichum oldhamii has beautiful foliage and attractive bright yellow flowers. However, breeding of T. oldhamii by conventional sexual hybridization may be difficult because sexual reproduction of this species is very rare. In the present study, plant regeneration systems via both direct and indirect formation of adventitious shoots from leaf explants were established as the first step toward breeding T. oldhamii by using biotechnological techniques. Adventitious shoots were formed efficiently on medium containing $0.1mg\;l^{-1}$ benzyladenine. Histological observation showed that shoot formation on this medium occurred directly from leaf epidermal cells without callus formation. On the other hand, leaf explants formed calluses on medium containing $0.1mg\;l^{-1}$ 2,4-dichlorophenoxyacetic acid. The calluses could be maintained by monthly subculturing to fresh medium of the same composition. When the calluses were transferred to plant growth regulator-free medium, they formed adventitious shoots. Directly and indirectly formed shoots rooted well on medium containing $0.1mg\;l^{-1}$ indole-3-butyric acid. Plantlets thus obtained were successfully acclimatized and grew vigorously in the greenhouse. Flow cytometry analysis indicated that no variation in the ploidy level was observed in plants regenerated via direct shoot formation, whereas chromosome doubling occurred in several plants regenerated via indirect shoot formation. Regenerated plants with the same ploidy level as the mother plants showed almost the same phenotype as the mother plants, whereas chromosome-doubled plants showed apparent morphological alterations: they had small and crispate flowers, and round and deep green leaves.

• 한국멀티미디어학회논문지
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• 제15권1호
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• pp.166-178
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• 2012

감성 세대로의 진입에 따라 소비자들의 선호 색을 반영한 자동차 선호 색상을 개발하려는 연구가 진행되고 있다. 본 연구에서는 제품 디자인과 소비 심리에 영향을 줄 수 있는 계층별 선호색과 감성 이미지에 의한 자동차 색에 대하여 조사 하였다. 조사 대상은 연령대 별, 성별에 따라 남, 여 20대, 30대, 40대, 50대 이상을 기준으로 8분류로 나누어 각각 30명씩 실시하였다. 선호 색상은 검은색, 흰색, 하늘색, 보라색, 분홍색, 주황색 순으로 나타났으며 검은색, 흰색, 회색을 제외한 색상은 파란색, 노란색, 보라색 순으로 나타났다. 감성적인 자동차 색상을 색상으로 보여주었을 때와 색상으로 보여주지 않았을 때 차이가 있음을 알 수 있었다. 이는 좋아하는 색상은 감성에 따르고 있으나 실제로 자동차 이미지가 보수적으로 인식되어 밝은 유채색 차의 선택을 주저하고 있음을 보여준다. 즉 아직은 감정표출에 있어 소극적임을 보여주고 있는 것이다.

• 한국어류학회지
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• 제21권4호
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• pp.283-286
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• 2009

고려홍어의 모식산지인 한반도 남서해안에서 포획한 암컷개체의 자궁에서 직접 적출한 난각 2개를 새롭게 기재하였다. 본종의 난각은 다른 종과 비교하여 보통 크기이고, 뿔(horn)을 포함한 전장은 124.5~133.7 mm, 뿔을 제외한 주부(main portion)의 체장은 102.8~109.4 mm, 최대폭은 41.0~42.2 mm이다. 측면에서 볼 때 배쪽으로 불룩한 비대칭이고, 표면은 비교적 부드러운 편이지만 세로로 미세한 고랑(longitudinal striations)은 뚜렷하고, 매우 발달한 섬유다발(견상사(絹狀絲) silky fibers)로 덥혀 있다. 앞뒤의 apron은 뚜렷하며, 네 뿔은 짧고 튜브모양이고 그 횡단면은 타원형이고, 앞쪽 뿔은 안쪽으로 심하게 구부러져 있으며, 호흡공(respiratory fissure)은 네 뿔의 끝부분에 위치한다. 신선한 상태에서 밝은 갈색이다.

• 한국의상디자인학회지
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• 제18권2호
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• pp.115-125
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• 2016

This study analyzes the characteristics of the motifs and colors in flower and bird paintings which contain and present Korea's unique aesthetics based on its richest data among all other types of Korean folk paintings. With a theoretical exploration of folk paintings and an analysis of the motifs and colors, textile design with an aim to present highly valued korean modern fashion design was developed. Two hundred and seventy flower and bird paintings were selected from relevant materials, resulting in an extraction of 5,068 colors. A palette of representative colors was made based on densely distributed hues and tones. The research can be summarized as follows: Flower and bird paintings contain flowers, birds, trees and animals and the motifs in the paintings stand for spirituality, philosophies from different times and other symbols of our nation. Each of the motifs in these paintings is a prayer for good luck, especially conjugal harmony and fecundity to bring about happiness and richness. Colors in flower and bird paintings are characteristic of the order: YR(25.5%)>R(19.2%)>Y(10.6%)>PB(9.2%)>G(7.9%), with low-chromatic YR and highly bright R taking up a larger percent. The order of tones is: d(13.5%)>dk (10.5%)>s(10.4%)>dkg(10.0%)>sf(9.1%)and the tone is dark in general. YR, taking up the largest percent, was dull, light, and soft. As for the achromatic colors, the order is: W(5.2%)>Gy(1.9%)>Bk(0.7%). Based on the above analyzed color data, those colors which were high in their hue and tone were extracted to present representative colors. In this way, representative colors like yellow, yellowish red, red, green, and purplish blue were extracted. This was the basis to present motifs and colors originating from subjects of folk paintings in various patterns using Illustrator CS6, to create modern fashion design.

• 한국조명전기설비학회지:조명전기설비
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• 제11권3호
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• pp.63-70
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• 1997

본 연구는 대학생들의 색기호를 알아 보고 색명에 대한 인지도를 파악하는데 목적을 두고 있다. 본 연구결과에 의해 다음과 같은 점을 알게 되어었다. 첫째, 대학생들이 선호하는 색은 파랑>보라>흰색·녹색 순이며 혐오하는 색은 무채색>황토색>빨강의 순이다. 둘째, 가구색으로는 갈색계통을 선호하고 빨강을 혐오하며, 의복색으로는 흰색을 선호하고 빨강을 혐오한다. 셋째, 계절의 이미지색으로 봄은 노랑, 여름은 파랑, 가을은 갈색, 겨울은 흰색이라고 생각하며 자신의 이미지 색은 푸른색이 가장 많았다. 넷째, 색명에 대한 인지도는 검정색>회색>빨강>녹색 순으로 인지도가 높으며 남색>다홍>연지>감청이 인지도가 낮았다. 또한 무채색계열>중성색계열>난색계열>한색계열의 순으로 색명 인지도가 높음이 밝혀졌다.

• 한국식품위생안전성학회지
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• 제5권3호
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• pp.131-138
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• 1990

Aflatoxins is a chemically diverse group of toxic secondary metabolites that are produced by fungi and often occur in agricultural commodities. Because of their wide range of toxic effects, Aflatoxins cause severe economic losses to farmers and livestock producers and pose a health to human consuming contaminated foods. Long term prospects for biotechnological control of Aflatoxins require elucidation of the specific steps and regulation of their biosynthetic pathways . Aflatoxin determinations can be approached many ways. It is essential to safely handle all experimental materials associated with aflatoxin analysis or aflatoxigenic fungi Visual screening of suspect samples, base on the presence of conidial head of the aspergillus flavus group, and screening samples for the presence of bright greenish yellow flourescence are not chemical tests and such screening techniques may allow aflactoxin contaminated lots into commerce. Microcolumn screening procedures should always be used in conjunction with a quantitative method. Several thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) methods are suitable for quantitation and are in general use. Immunochemical Methods such as the ELISA or affinity column chromatography methods are being rapidly developed. The chemical and immunochemical methods can be reliable if care is taken, using suitable controls and personnel that are well trained . All analytical laboratories should stress safety and include suitable analytical validation procedure. Especially a worldwide enquiry was undertaken in recent to obtain up-to-date information about aflatoxin legislation in as many countries of the world as possible. The information concerns aflatoxin in foodstuffs. aflatoxin MI in dairy products, aflatoxins in animal feedstuffs. Limits and regulations for aflatoxin have been expended in recent with more countries having legislation on subject, more products, and more aflatoxins covered by this legislation.