• Biomolecules & Therapeutics
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• 제30권5호
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• pp.418-426
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• 2022

Chimeric antigen receptor T (CAR-T) cell therapy is one of the promising anticancer treatments. It shows a high overall response rate with complete response to blood cancer. However, there is a limitation to solid tumor treatment. Additionally, this currently approved therapy exhibits side effects such as cytokine release syndrome and neurotoxicity. Alternatively, bispecific antibody is an innovative therapeutic tool that simultaneously engages specific immune cells to disease-related target cells. Since programmed death ligand 1 (PD-L1) is an immune checkpoint molecule highly expressed in some cancer cells, in the current study, we generated αCD3xαPD-L1 bispecific antibody (BiTE) which can engage T cells to PD-L1⁺ cancer cells. We observed that the BiTE-bound OT-1 T cells effectively killed cancer cells in vitro and in vivo. They substantially increased the recruitment of effector memory CD8⁺ T cells having CD8⁺CD44⁺CD62L^low phenotype in tumor. Interestingly, we also observed that BiTE-bound polyclonal T cells showed highly efficacious tumor killing activity in vivo in comparison with the direct intravenous treatment of bispecific antibody, suggesting that PD-L1-directed migration and engagement of activated T cells might increase cancer cell killing. Additionally, BiTE-bound CAR-T cells which targets human Her-2/neu exhibited enhanced killing effect on Her-2-expressing cancer cells in vivo, suggesting that this could be a novel therapeutic regimen. Collectively, our results suggested that engaging activated T cells with cancer cells using αCD3xαPD-L1 BiTE could be an innovative next generation anticancer therapy which exerts simultaneous inhibitory functions on PD-L1 as well as increasing the infiltration of activated T cells having effector memory phenotype in tumor site.

• 한국지반공학회논문집
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• 제23권4호
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• pp.185-197
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• 2007

한계해석법인 상 하계법은 점착성, 점착성-마찰성, 마찰성분만 가지는 지반에서의 주로 얕은 터널에 대한 안정수를 구하기 위해 발전되어 왔다. 그러나 점성이 없고 마찰성분만 존재하는 지반에서의 비교적 깊은 터널에 대한 이러한 해석법의 연장은 현재까지 그 연구가 드물게 진행되어왔다. 따라서 본 연구는 이러한 상황에서의 터널붕괴하중을 구하기 위한 근사적인 해석법으로 응력불연속장에 근거하는 하계법과 동적 파괴메카니즘에 근거하는 상계법을 각각 제안하였다. 이러한 해석법에 의한 터널붕괴하중은 수치해석과 기존의 경계해석법과 비교되었으며 특히, 터널 수평축 상에 위치하는 유한지반요소들에 대한 유한요소해석 결과와 잘 일치됨을 보여 주었다.

• Journal of Digestive Cancer Research
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• 제5권1호
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• pp.62-65
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• 2017

2013년 실제 임상에 Gemcitabine과 nab-paclitaxel 병용요법이 적용된 후 NCCN Guideline에서 절제 불가능한 췌장암 환자에서 일차적으로 선택할 수 있는 약제로 제시하고 있다. 이 병용요법으로 인한 가장 흔한 Grade 3 부작용으로는 호중구감소증, 피로, 말초신경병증이 보고되었으며, 심장독성은 흔하지 않다. 본 증례에서는 심장표지자의 상승 및 심초음파에서의 우측관상동맥의 허혈 손상이 확인되어 병용요법으로 인한 심장 허혈 손상 및 심낭삼출물이 발생하여, 심장성 쇼크로 사망하였을 것으로 추정해 볼 수 있다. Gemcitabine과 nab-paclitaxel에 의한 심장 허혈 손상의 더 많은 증례 보고 및 연구가 필요하며, 병용요법을 투여 받는 환자들에 대한 심장독성에 대하여 주의 깊은 관찰이 필요할 것으로 생각된다.

• 한국경영과학회지
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• 제33권3호
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• pp.1-16
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• 2008

This paper considers a scheduling problem to minimize the total tardiness in the two-stage assembly-type flowshop. The system is composed of multiple fabrication machines in the first stage and a final-assembly machine in the second stage. Each job consists of multiple tasks, each task is performed on the fabrication machine specified in advance. After all the tasks of a job are finished, the assembly task can be started on the final-assembly machine. The completion time of a job is the time that the assembly task for the job is completed. The objective of this paper is to find the optimal schedule minimizing the total tardiness of a group of jobs. In the problem analysis, we first derive three solution properties to determine the sequence between two consecutive jobs. Moreover, two lower objective bounds are derived and tested along with the derived properties within a branch-and-bound scheme. Two efficient heuristic algorithms are also developed. The overall performances of the proposed properties, branch-and-bound and heuristic algorithms are evaluated through numerical experiments.

• 한국정보과학회논문지:소프트웨어및응용
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• 제37권7호
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• pp.584-589
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• 2010

시계열 패턴 인식에 일반적으로 많이 사용되는 동적인 타임 워핑 알고리즘은 대부분의 연산시간을 상관표를 작성하는데 소비한다. 그리고 이 연산시간을 줄이고자 전역 경로 제약조건을 설정하여 연산범위에 제한을 두는데, 이것은 패턴의 내용을 고려하지 않은 시간축에 의한 제한이다. 따라서 본 논문에서는 패턴의 형태에 따라 적응적으로 전역 경로 제약조건을 설정하여 보다 효율적으로 패턴인식을 수행하는 분기 한정적인 동적 타임 워핑 알고리즘을 제안한다. 제안된 방법의 성능 평가를 위한 실험에서는 분기 한정적인 동적 타임 워핑 알고리즘이 기존의 동적 타임 워핑 방법과 경로 거리는 유사하면서 연산 시간이 보다 개선되었음을 확인할 수 있었다.

• 한국정보통신학회논문지
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• 제10권7호
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• pp.1188-1195
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• 2006

본 논문은 개량된 분기한정법(branch-and bound method)을 이용하여 고속연산이 가능한 2차원 상태공간 디지털 필터의 설계방법을 제안하였다. 설계된 디지털 필터가 갖는 계수행렬의 모든 요소 값들은 오직 2개의 2의 멱수 (powers-of-two) 의 합으로만 표현되어진다. 따라서 제안된 방법으로 설계한 2 차원 상태공간 디지털필터는 간단한 가산연산만으로 수행이 이루어지므로 고속연산이 가능하고, 또한 구조가 간단하며 저가격으로 실현이 가능하다는 장점을 가진다 . 본 논문에서 제안된 방법의 타당성을 설계예시를 통해 고찰한 결과, 설계된 디지털 필터의 근사오차와 군지연특성이 동일한 설계사양으로 연속계수공간에서 설계되어진 디지털필터와 비교하여 유사한 결과를 얻을 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제33권2호
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• pp.529-534
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• 2012

The fluorescence of 4',6-diamidino-2-phenylindole (DAPI) bound to DNA at a [DAPI]/[DNA base] ratio of 0.005 was quenched by meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP) or cis-bis(N-methylpyridinium-4-yl)porphyrin (BMPyP) when both DAPI and either porphyrin spontaneously bound to the same DNA strand. The quenching was investigated using the "one-dimensional inner sphere" and the "F$\ddot{o}$rster resonance energy transfer" (FRET) models. Total quenching occurred when DAPI and TMPyP were up to 19.3 base pairs or $66\AA$ apart. BMPyP could quench the fluorescence up to 13.9 base pairs or $47\AA$. TMPyP, which intercalated between the DNA base-pairs, appeared to be a better acceptor than BMPyP, which stacked along the DNA stem. The higher quenching and higher resonance energy transfer efficiency of TMPyP was due to the larger overlap integral between its absorption spectrum and the emission spectrum of DNA-bound DAPI.

• 대한기계학회논문집A
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• 제21권9호
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• pp.1364-1372
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• 1997

In this paper, the forging of helical gears has been investigated. Punch is tooth-shaped as is the die insert. The punch compresses a cylindrical billet placed in a die insert. As a consequence the material of billet flows into the tooth region. The forging has been analysed by using the upper-bound method. A kinematically admissible velocity field has been developed, wherein, an involute curve has been introduced to represent tooth profile of the gear. Numerical calculations have been carried out to investigate the effects of various parameters, such as module, number of teeth, helix angle and friction factor on the forging of helical gears. Some forging experimentswere carried out with aluminum alloy to show the validity of the analysis. Good agreement was found between the predicted values of the forging load and obtained from the experimental results.

• Journal of Communications and Networks
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• 제17권1호
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• pp.21-26
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• 2015

The general wiretap channel with noiseless feedback is first investigated by Ahlswede and Cai, where lower and upper bounds on the secrecy capacity are provided in their work. The upper bound is met with equality only in some special cases. In this paper, we study a special case of the general wiretap channel with noiseless feedback (called non-degraded wiretap channel with noiseless feedback). Inner and outer bounds on the capacity-equivocation region of this special model are provided. The outer bound is achievable if the main channel is more capable than the wiretap channel. The inner bound is constructed especially for the case that the wiretap channel is more capable than the main channel. The results of this paper are further explained via binary and Gaussian examples. Compared with the capacity results for the non-degraded wiretap channel, we find that the security is enhanced by using the noiseless feedback.

• 소성∙가공
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• 제8권4호
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• pp.374-383
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• 1999

To determine the flow stress of semi-solid materials, a new combined method has been studied by experimental and analytic technique in the current approach. Using backward extrusion experiment and its numerical analysis, the characterization scheme of semi-solid materials according to the change of initial solid volume fraction has been proposed. Because that solid volume fraction is sensitive to temperature change, it is required to precisely control the temperature setting. Model materials can guarantee the establishment of material characterization technique from the noise due to temperature change. Thus, clay mixed with bonded abrasives was used for experiment and the change of initial solid fraction was copied out through the variation of mixing ratio. Upper bound method was adapted to increase in efficiency of the calculation in numerical analysis and new kinematically admissible velocity field was employed to improve the accuracy of numerical solution. It is thought that the material characterization scheme proposed in this study can be applied to not only semi-solid materials, but also other materials that is difficult to obtain the simple stress state.