• Mycobiology
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• v.41 no.3
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• pp.167-169
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• 2013

Black spot of sweet persimmon, caused by Alternaria alternata, occurred in an orchard in Gyeongnam province, Korea in 2012. The symptom was appearance of 0.5 to 4 cm black spots on the surface of fruit. The pathogen was isolated from flesh of disease lesions. The causal agent was identified as A. alternata by morphological characteristics and sequencers of the internal transcribed spacer (ITS) 1 and ITS4 regions of rRNA. Artificial inoculation of the pathogen resulted in development of disease symptoms and the re-isolated pathogen showed characteristics of A. alternata.

• Research in Plant Disease
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• v.10 no.3
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• pp.183-187
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• 2004

Black spot disease on sweet persimmon (Diospyros kaki cv. Fuyu) was found from the farmer's refregerated ware house located in Dong-eup, Changwon city, Gyeongnam province, Korea, 2002. The typical symptoms of the disease was appeared black spot on surface of fruits. Colonies of causal fungus were developed velvety and later turned olivaceous black to sooty black in color on PDA. Conidia were pale brown to golden brown in color and ovoid, obclavate to obpyriform, rarely ellipsoid in shape. and were 12∼46 ${\times}$ 6∼12 ${\mu}{\textrm}{m}$ in size. Septa were 3∼5 transvers, 1∼2 longitudinal or oblique. Conidia were mostly beakless and generated chains by means of secondary conidiophores. Conidiophores were pale to mid brown in color and 42∼95 ${\times}$ 3∼5 ${\mu}{\textrm}{m}$ in size. The optimum temperature for growth of the fungus was about 25∼3$0^{\circ}C$. This is the first report on the black spot of sweet persimmon caused by Alternaria alternata (Fr.) Keissler in Korea.

• Research in Plant Disease
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• v.8 no.4
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• pp.254-257
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• 2002

It was conducted to investigate an effective control measure of the black necrotic leaf spot disease on pear, The disease commonly occurred in major cultivation areas of Korea revealed 5.6 ％ infection rate on the widely growing cultivar Niikata. The disease substantially reduced yield and quality of pear fruits, however, it has never been controlled by chemicals. Renewal of the pear trees to resistant cultivar was found to be the most effective to control the disease. As a result, the disease did not developed for 2 years on the diseased Niikata that was top-grafted with resistant cultivars Soowhang and Whasan. lop-grafting with resistant cultivars was recommended far the pear trees showing over 80％ infection rate and lower than 70% fruit yield compare to standard production.

• Journal of Ginseng Research
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• v.42 no.3
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• pp.304-311
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• 2018

Background: Ginseng black spot disease resulting from Alternaria panax Whuetz is a common soil-borne disease, with an annual incidence rate higher than 20-30%. In this study, the bacterial strains with good antagonistic effect against A. panax are screened. Methods: A total of 285 bacterial strains isolated from ginseng rhizosphere soils were screened using the Kirby-Bauer disk diffusion method and the Oxford cup plate assay. We analyzed the antifungal spectrum of SZ-22 by confronting incubation. To evaluate the efficacy of biocontrol against ginseng black spot and for growth promotion by SZ-22, we performed pot experiments in a plastic greenhouse. Taxonomic position of SZ-22 was identified using morphology, physiological, and biochemical characteristics, 16S ribosomal DNA, and gyrB sequences. Results: SZ-22 (which was identified as Brevundimonas terrae) showed the strongest inhibition rate against A. panax, which showed 83.70% inhibition, and it also provided broad-spectrum antifungal effects. The inhibition efficacies of the SZ-22 bacterial suspension against ginseng black spot reached 82.47% inhibition, which is significantly higher than that of the 25% suspension concentrate azoxystrobin fungicide treatment (p < 0.05). Moreover, the SZ-22 bacterial suspension also caused ginseng plant growth promotion as well as root enhancement. Conclusion: Although the results of the outdoor pot-culture method were influenced by the pathogen inoculum density, the cropping history of the field site, and the weather conditions, B. terrae SZ-22 controlled ginseng black spot and promoted ginseng growth successfully. This study provides resource for the biocontrol of ginseng black spot.

• Research in Plant Disease
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• v.9 no.3
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• pp.149-152
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• 2003

Black leaf spot disease occurred on Dendrobium phalaenopsis grown in farmer's fields located in Bonggangmyon, Gwangyang-eup, Jeonnam, Korea. Black small spots occurred on leaves at initial stage of infection and the infected leaves turned yellow from the tip. The yellow leaves were falled, resulting in stem blighting or eventual death of the entire plant. White mycelial colony of the causal fungus grown on potato dextrose agarturned dark violet later and optimum temperature for the mycelial growth was $25^{\circ}C$. The causal fungus isolated from the black leaf spot on D. phalaenopsis was identified as Fusarium moniliforme based on the mycological characteristics and pathogenicity. The fungus also caused same symptoms on leaves of Phalaenopsis sp. and Cymbidium sp. as well sa D. phalaenopsis by wound inoculation. This is the first report on black leaf spot of D. phalaenopsis caused by F.moniliforme in Korea.

• Korean journal of applied entomology
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• v.23 no.1 s.58
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• pp.7-14
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• 1984

Recently, a symptom similiar to black spot caused by Alternaria kikuchiana on the pear leaves is prevailing in Naju, Chonnam Province. This experiment was conducted in order to clarify causal agent and to survey present status on it. Disease lesions on the leaves were brown or black brown in color, circle or irregular circle in shape, $2\~6mm$ in diameter, and faintly zonal. Central part of lesions was gradually changed to greyish-white. The causal fungus was identified as Alternaria kikuchiana Tanaka causing black spot of pear reported previously on variety I-sib-se-gi (Nijisseki) of pear. However, this disease differed from that of typical black spot reported already in some aspects; It occurred more highly on matured leaves than on young ones. And it occurred severely in varieties Shin-heung, Shin-ko, Man-sam-gil(Okusankichi) etc. which have been reported as resistant varieties, while slightly in varieties Cho-ok(Hayadama), Park-da-cheung (Hakadahare) etc. which have been known as susceptible ones. From the results described above, it is considered that this disease is due to the differentiation in pathogenicity of Alternaria kikuchiana Tanaka in that area.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.4 no.1
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• pp.103-108
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• 1968

Resistance to various diseases of Korean native varieties were investigated in field. The results obtained are summarized as follows: 1) The varieties Ubangtchio and Osib-eubthio were moderately resistant to black shank, but Suantchio, Usultchio and Gwangtchio were slightly resistant. Dixie Bright 101, $H_2$ and Bright Yellow 4 were more resistant to black shank than any of the Korean native varieties. 2) Mokgitchio, Ubangtchio and Osib-eubtchio were moderately resistant to black root rot, but Useultchio Suantchio and Hoetchio were susceptible than Others. 3) Muktchio, Hoetchio and Ubangtchio were slightly resistant to wild fire. 4) Osib-ebthio was highly resistant to brown spot, but Ubangtchio, Hyangtchio and Mokgitchio were moderately resistant and Useultchio was susceptible to brown spot, but it was resistant than Bright ellow 4 or Bernhart 1000-1. 5) Hyangtchio was slightly resistant to CMV-Y. According to these results, except Virus disease, Ubangtchio was resistant to all of the above mentioned diseases. Osib-eubtchio, Hoetchio, Hyangtchio and Suantchio showed proferable disease resistant and these might be well utilized as breeding materials.

• Korean Journal Plant Pathology
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• v.14 no.1
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• pp.52-61
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• 1998

This paper is the fifth report about the fungal diseases of economic resource plants observed newly in Korea. It contains short descriptions on symptoms, occurrence conditions, pathogens, and some phytopathological notes for each of 10 fungal plant diseases. They are identified as leaf spot of Adenophora triphylla var. japonica by Septoria lengyelii, leaf spot of Calystegia soldanella by S. convolvuli, leaf spot of Campanula punctata by S. campanulae, leaf spot of Codonopsis lanceolata by S. codonopsidis, leaf spot of Geum japonicum by s. gei, black spot of Oenanthe javanica by s. oenanthes, leaf spot of Oenothera odorata by S. oenotherae, angular leaf spot of Rehmannia glutinosa by S. digitalis, brown spot of Rubus crataegifolius by s. rubi, and leaf spot of Viola verecunda by S. violae-palustris, respectively.

• Mycobiology
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• v.43 no.3
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• pp.343-346
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• 2015

In 2006~2010, leaf spot symptoms, that is, small, yellow spots that turned into dark brown-to-black lesions surrounded by a yellow halo, were observed on Cymbidium spp. in Gongju, Taean, and Gapyeong in Korea. A Fusarium species was continuously isolated from symptomatic leaves; in pathogenicity testing, isolates caused leaf spot symptoms consisting of sunken, dark brown lesions similar to the original ones. The causal pathogen was identified as Fusarium subglutinans based on morphological and translation elongation factor 1-alpha sequence analyses. This is the first report of F. subglutinans as the cause of leaf spot disease in Cymbidium spp. in Korea.

• Mycobiology
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• v.48 no.3
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• pp.233-239
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• 2020

A small-spored Alternaria was found from black spots of storaged Koerle pear (Pyrus sinkiangensis), one of the economically important fruit in Xinjiang province, China. The morphology is similar to A. limoniasperae but obviously different in secondary conidiophores and conidial septa. A phylogenetic analysis using sequence datasets of ITS, GAPDH, TEF1, RPB2, Alt a1, OPA10-2, and EndoPG genes revealed that it belonged to the Alternaria alternata complex group. Pathogenicity tests illustrated that the fungus was the causal pathogen of black spot on Koerle pear fruit.