• Title/Summary/Keyword: bio materials

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Dyeing of Silk Fabric with Aqueous Sorghum Extract (수수 추출물에 의한 견직물의 염색)

  • Lee, Sung-Eun;Bae, Do-Gyu;Jung, Yang-Sook
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.43-43
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    • 2012
  • 본 연구에서는 대량으로 손쉽게 구 할 수 있는 식물염료를 확보하여 새로운 천연색소자원을 탐색하기 위해서 진행되었으며, 시료의 최적 추출 조건을 확립하고 추출된 색소의 실크에 대한 염색 특성을 살펴 최적 염색조건을 설정하고, 염색견뢰도를 평가하여 수수 등겨 추출물의 천연색소로의 활용에 대한 기초 자료를 마련하고자 하였다. 수수 색소 추출 시 생산성과 편리성을 고려하여 추출은 pH 4, 추출온도 $60^{\circ}C$, 추출시간 24시간이 적정조건으로 제시되었으며, 수수 추출물에 함유되어 있는 탄닌의 최대흡수파장은 282nm로 나타났다. 견직물의 pH에 따른 염색 특성은 pH가 증가할수록 K/S값이 증가하여 염착량이 증가하였으며, 염색온도가 높을수록 염색시간이 길어질수록 염착량이 증가하는 경향을 보여주고 있다, 염색온도가 높을수록 염색시간이 경과될수록 색상은 $Y{\rightarrow}YR$로 변화되는 것을 알 수 있었으며 염색온도가 높을수록 채도가 증가하였다. 욕비가 클수록 염착량은 감소하였으나, 채도는 약간 증가하는 것을 알 수 있었다. 매염제 처리에 의한 색상변화에서는 Al, Ni 매염은 색상변화는 적었지만, Cu와 Fe 매염은 명도와 채도를 크게 감소시키고 아울러 색상도 Cu는 적갈색으로 Fe는 진회색으로 변화되었다. Sn과 Ti 매염에 의해서 명도는 약간 감소하고 채도는 약간 상승하여 연한 주황색으로 색상이 변화하였다. 설정된 조건에서 염색된 염색포의 일광견뢰도는 2~3급, 세탁견뢰도 변퇴색은 2~3급, 오염은 4~5급으로 나타났다. Fe로 매염된 염색포의 일광견뢰도는 증가하여 3~4급으로 나타났으며, 매염제 처리에 의한 세탁 견뢰도는 같거나 약간 증가되는 것으로 나타났다.

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Natural Dyeing with Aqueous Black Bean Seed Coat extract (검정팥 종피 추출물에 의한 천연염색)

  • Cha, Hae-Suk;Bae, Do-Gyu;Jung, Yang-Sook
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.42-42
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    • 2012
  • 팥에 함유되어있는 색소와 관련된 연구 중에서는, 검정팥의 색소성분을 분리하여 delphinidin-3-glucoside(D-3-G)로 보고한 Sasanuma의 연구와 검정팥 껍질속에 포함된 안토시아닌의 함량을 평가한 Yoshida의 연구 결과 등이 있다. 본 연구에서는 검정팥 종피에서 색소를 추출하여, 적정 염색조건을 설정하였으며, 매염제별 색상변화 및 견뢰도를 측정하는 과정을 통해 염색 특성을 파악하여 새로운 천연염재로서의 활용 가능성을 진단하였을 뿐만 아니라 응용 연구를 위한 기초자료의 확보에 주안점을 두었다. pH에 따른 염색성에서는 견직물의 경우 pH 4에서 가장 염착량이 많았고, 면직물의 경우에는 주어진 범위내에서는 pH가 높을수록 염착량이 많았다. 염색온도와 시간에 따른 염색성은, 견직물의 경우, 염색온도 $80^{\circ}C$에서는 시간이 경과할수록 K/S 값이 크게 나타났으며, 면직물의 경우도 견직물과 비슷한 결과로 온도가 높아질수록 염색시간이 경과될수록 K/S 값이 크게 나타났다. 견직물에 대한 매염제 종류별 표면색의 변화에서는 Fe 매염포만 $YR{\rightarrow}Y$ 로 변화되었을 뿐 다른 매염제에서는 매염 후에도 색상 변화는 크지 않았다. 매염처리 전 견직물의 일광 견뢰도는 무매염이 4~6등급, Al 처리포는 4~5등급, Cu와 Sn은 3~4등급으로 나타났고 Fe는 2~3등급으로 가장 낮게 나타났으며, 세탁견뢰도에서는 무매염 2등급, 매염제 처리의 경우 2~3등급으로 나타났다. 무매염 면직물의 일광견뢰도는 1~2 등급, Fe 매염은 2~3등급, Cu 2등급 Al과 Sn은 1~2등급으로 매염처리에 의해 전혀 개선되지 않았으며, 세탁견뢰도의 경우 Cu매염이 4등급으로 양호하게 나타났으며 무매염, Al, Sn과 Fe는 3등급으로 나타났다.

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Insecticidal and Acaricidal Activities of Plant Extracts (식물체 추출물의 살충 및 살비활성)

  • Kwon, Min;Lee, Seong-Baek;Ahn, Young-Joon;Park, No-Jung;Cho, Kwang-Yun
    • Applied Biological Chemistry
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    • v.37 no.6
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    • pp.492-497
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    • 1994
  • Extracts of 43 species of plants were tested for their insecticidal and acaricidal activities against six species of insect pests and one mite species. The methanol extract of Ginkgo biloba leaves selectively was found to have potent insecticidal activity against Nilaparvata lugens (Stal), whereas steam distillate of Thujopsis dolabarata var. hondai sawdust showed potent insecticidal activities with a broad spectrum. The methanol extract of Pinus densiflora leaves and steam distillate of T. dolabrata var. hondai sawdust exhibited potent activities against Tetranychus urticae (Koch).

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Larvicidal and Antifeeding Activities of Oriental Medicinal Plant Extracts against Plutella xylostella and Spodoptera litura (배추좀나방과 담배거세미나방 유충에 대한 한방식물체 추출물의 살충 및 섭식저해활성)

  • Kwon, Hyung-Wook;Ahn, Young-Joon;Kweon, Jeong-Hyun;Lee, Sang-Gil;Byun, Byung-Ho
    • Applied Biological Chemistry
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    • v.37 no.6
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    • pp.503-508
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    • 1994
  • Methanol extracts from 45 species of oriental medicinal plants belonging to 17 families were subjected to a screening test for their larvicidal and antifeeding activities against two species of lepidopteran larvae, diamondback moth (Plutella xylostella L.) and tobacco cutworm (Spodoptera litura F.) by a leaf-dipping method. At a concentration of 8,000 ppm, methanol extract of Corydalis turrschaninovii roots only was found to have potent larvicidal activity against S. litura. At 5,000 ppm, strong antifeeding activity against both larvae was observed from the extracts of Clerodendron trichotomum, C. trichotomum var. esculentum, Inura helenium, Arctium lappa, Artemisia messerschmidtiana, Anthriscus sylvestris, Angelica dahurica, and C. turrschaninovii.

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Early gonadal maturation and vitellogenin mRNA expression in Siberian sturgeon Acipenser baerii cultured in a semi-closed water recirculating system in Korea

  • Park, Chulhong;Gong, Seung Pyo;Choi, Youn Hee;Kim, Ki Hong;Nam, Yoon Kwon
    • Journal of Animal Reproduction and Biotechnology
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    • v.35 no.3
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    • pp.279-288
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    • 2020
  • Changes of gonadal morphology and mRNA expression patterns of vitellogenin were investigated in Siberian sturgeon Acipenser baerii (Chondrostei) during its early gonadal maturation period. Early differentiations and morphological transitions of both ovaries and testes appeared to occur actively until the age of 3 years, however from then on, the maturation patterns to full maturity were largely gender-dependent, in which males showed a faster progression of maturation than did females while females experienced a steady-state progress with a lagged interval before entering the final maturation. Expression of vitellogenin mRNAs are closely correlated with transitional patterns of gonadal appearances. In both females and males, hepatic mRNA levels of vitellogenin exponentially increased in the earliest interval (up to 1-year-old). However, in subsequent periods, vitellogenin expression in females continued to increase with age, whereas in males, the expression stabilized at a younger age. Nevertheless, at the age older than or equal to 7-year-old, fully matured individuals showed a quite low level of vitellogenin expression in both females and males. Collectively, results from this study could be useful as a fundamental guideline to address the gonad maturation of this sturgeon species, which is helpful for making practical decisions about farming practices and management for caviar production on local sturgeon farms.

Quantitation of fructo- and inulo-oligosaccharides by high performance liquid chromatography (High performance liquid chromatography에 의한 fructo 및 inulo올리고당의 정량)

  • Kang, Su-Il;Han, Jong-In;Kim, Kyoung-Youn;Oh, Sun-Jin;Kim, Su-Il
    • Applied Biological Chemistry
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    • v.36 no.4
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    • pp.310-314
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    • 1993
  • High performance liquid chromatographic method using a TSK-gel amide 80 column and isocratic elution with acetonitrile-water (63 :35 ;v/v) mixture was used for the separation and the quantitation of fructo (GF2-GF7)- and inulo-oligosaccharides (F2-F4). Retention time of each standard carbohydrate was highly reproducible. Standardization curves obtained by plotting the peak areas against the amounts of each carbohydrate showed very high coefficient of determination$({\ge}0.9884)$ and similar slopes, and a wide range of y-intercepts. Our results suggest the use of each Pure oligosaccharide for its own standardization curve instead of using a certain carbohydrate as an internal standard.

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Molecular Cloning and Expression of a Novel Cuticle Protein Gene from the Chinese Oak Silkmoth, Antheraea pernyi

  • Kim Bo Yeon;Park Nam Sook;Jin Byung Rae;Kang Pil Don;Lee Bong Hee;Seong Su Il;Hwang Jae Sam;Chang Jong Su;Lee Sang Mong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.1
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    • pp.11-17
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    • 2005
  • In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.

Effect of MnO2 Addition on Microstructure and Piezoelectric Properties of 0.95(Na0.5K0.5)NbO3-0.05CaTiO3 Piezoelectric Ceramics

  • Kim, Jong-Hyun;Seo, In-Tae;Hur, Joon;Kim, Dae-Hyeon;Nahm, Sahn
    • Journal of the Korean Ceramic Society
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    • v.53 no.2
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    • pp.129-133
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    • 2016
  • $MnO_2$ was added to the $0.95(Na_{0.5}K_{0.5})NbO_3-0.05CaTiO_3$ (NKN-CT) ceramics in order to promote the densification and improve the poling efficiency by increasing the resistance of the specimens. Densification and abnormal grain growth occurred in the $MnO_2$-added NKN-CT ceramics sintered at $1020^{\circ}C$, indicating that $MnO_2$ assisted the liquid-phase sintering of these materials. $Mn^{3+}$ ions were considered to enter the A-site of the matrix, thereby producing the free electrons, which interacted with the holes resulting from the evaporation of alkali ions. This interaction results in an increase in the resistance of the specimens. The increased resistance improved the poling efficiency and, hence, the dielectric and piezoelectric properties of the NKN-CT ceramics. A few of the $Mn^{3+}$ ions that entered the B-site of the NKN-CT matrix led to a slight increase in the mechanical quality factor.

Effects of Photoperiod Manipulation on Growth Performance and Hematological Responses of Juvenile Caspian Roach Rutilus rutilus caspicus

  • Shahkar, Erfan;Kim, Dae-Jung;Mohseni, Mahmoud;Khara, Hossein;Yun, Hyeonho;Bai, Sungchul C.
    • Fisheries and Aquatic Sciences
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    • v.18 no.1
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    • pp.51-56
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    • 2015
  • A 8-week trial was conducted to evaluate the effects of photoperiod manipulation on the growth performance and hematological parameters of juvenile Caspian roach, Rutilus rutilus caspicus (average weight $1.46{\pm}0.12g$ mean${\pm}$SD) reared under five photoperiods (24 h Light, 24L; 18 h Light & 6 h Dark, 18L:6D; 12 h Light & 12 h Dark, 12L:12D; 6 h Light & 18 h Dark, 6L:18D; 24 h Dark, 24D) with constant light intensity 1,500 lx on the water surface. Triplicate of 20 fish were allocated into each of 15 fiberglass tanks of 50 L capacity and they were fed three times per day with the commercial feed (SFK, Co., Sari - Iran) contains 50.0% protein and 10.5% lipid. At the end of experimental period, final body weight, weight gain and specific growth rates of fish exposed to 24L were significantly higher than those of fish exposed to 12L:12D, 6L:18D and 24D (P < 0.05). Red blood cell and hemoglobin of fish exposed to 24L were significantly higher than those of fish exposed to 24D. No significant difference observed in hematocrit, white blood cell and plasma glucose among the different treatments groups. Therefore, these results demonstrated that the growth performance of juvenile Caspian roach can be significantly stimulated by using 24L and 18L:6D photoperiods without any measurable significant stress response such as plasma glucose concentration.

Effects of Salinity Changes on Hematological Responses in Juvenile Ship Sturgeon Acipenser nudiventris

  • Shahkar, Erfan;Kim, Dae-jung;Mohseni, Mahmoud;Yun, Hyeonho;Bai, Sungchul C.
    • Fisheries and Aquatic Sciences
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    • v.18 no.1
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    • pp.45-50
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    • 2015
  • A 10-day trial was conducted to evaluate the salinity tolerance and hematological responses of juvenile ship sturgeon Acipenser nudiventris under different salinity levels. A total of 240 fish averaging $6.2{\pm}0.13g$ (mean${\pm}$SD) were randomly distributed into 12 tanks in groups of 20, and each tank was then randomly assigned to one of three replicates of four different salinities at 0, 4, 8 and 12‰. At the end of 10 days of experimental period, plasma protein value of fish exposed to 0‰ and 4‰ salinities was significantly higher than those of fish exposed to 8‰ and 12‰ salinities (P < 0.05). Significantly higher values for plasma cortisol and glucose were obtained in fish exposed to 12‰ salinity compared to the fish exposed to 0‰ and 4‰ salinities (P < 0.05). Plasma potassium and sodium values increased significantly by increment of salinity levels (P < 0.05). Therefore, these results indicated that in natural environments, juvenile ship sturgeon, A. nudiventris in migratory populations could be able to migrate and adjust successfully into brackish water with a salinity of up to 8‰ without any short-term hematological stress responses.