International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Molecular Cloning and Expression of a Novel Cuticle Protein Gene from the Chinese Oak Silkmoth, Antheraea pernyi

  • Kim Bo Yeon (Department of Genomics, Proteomics and Bio-materials, Miryang National University) ;
  • Park Nam Sook (Department of Genomics, Proteomics and Bio-materials, Miryang National University, College of Natural Resources and Life Science, Dong-A University) ;
  • Jin Byung Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Kang Pil Don (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee Bong Hee (Graduate School of Biotechnology, Korea University) ;
  • Seong Su Il (Department of Biology, Collage of Natural Science, The University of Suwon) ;
  • Hwang Jae Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Chang Jong Su (Department of Life Science, College of Natural Science, Daejin University) ;
  • Lee Sang Mong (Department of Genomics, Proteomics and Bio-materials, Miryang National University)
  • Published : 2005.03.01
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Abstract

In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.

Keywords

References

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