• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.77-88
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• 2010

In order to ensure good animal health and performance, it is essential to produce forages with high feeding value and good hygienic quality. However, huge amounts of forages consumed by ruminants are contaminated with mold prior to harvest or during storage as hay, straw or silage. These mold can grow in forages only when nutrients are available, correct temperature exist, oxygen is present, and unbound water is available. Fungal 'species can be divided into two groups: field fungi and storage fungi. Field fungi invade the forages while the crop is still in the field, require high moisture conditions, and are such as species of Fusarium, Alternaria, Clodosporium, Diplodia, Gibberrella and Helminthosporium. Storage fungi invade forages during storage and need less moisture than field fungi. These such as species of Aspergillus and Penicillium usually do not occur any problem before harvest. Mold growth can spoil the nutritional aspects of the forages and also results in secondary metabolites that are highly toxic to animal, humans and plants. Moldy feeds are less palatable and may reduce dry matter intake. This, in turn, leads to a reduction of nutrition intake, reducing weight gains or milk production. Performance losses of 5 to 10 percent are typical with moldy feeds. Mycotoxins are toxic substances produced by fungi (molds) growing on crops in the field or storages. While greater than 400 mycotoxins have been chemically identified, the biological or veterinary medical impact of only several mycotoxins is known. Mycotoxins have attracted considerable attention as potential causes for poor performance and health disorders in domestic livestock. They can be carcinogenic, hepatotoxic, hematotoxic, immunosuppressive, estrogenic, or mutagenic. So, feeding moldy forages has adverse effects on animal health and milk consumers. Also, this author reported that rice straw hay was contaminated mycotoxigenic fungi such as Penicillium roqueforti and Fusarium culmorum in Korea. Therefore, it is an urgent need to develop an improved post harvest storage method to reduce nutrient loss and mycotoxin contamination of forages, which will have a positive impact on human health.

• Korean Journal of Animal Reproduction
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• v.24 no.3
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• pp.269-279
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• 2000

The present study was carried out to evaluate the effect of glycosaminoglycans added to the culture medium on the mouse embryo development to the blastocyst stage. In vitro fertilized mouse oocytes were cultured in Ham's F-10 supplemented with 10% FBS either in the absence or presence of 0.1, 0.5, 1.0 mg/$m\ell$ hyaluronic acid, chondroitin sulfate, and dermatan sulfate, respectively. After 4 days in culture, embryos developed to blastocysts were observed in all groups. There was a significant increase in blastocyst yield in the presence of hyaluronic acid and chondroitin sulfate (p<0.05), whereas dermatan sulfate was ineffective. Development to the blastocyst stage was best supported in 0.1, 0.5, 1.0 mg/$m\ell$ hyaluronic acid and 0.5mg/$m\ell$ chondroitin sulfate. It is concluded that hyaluronic acid and chondroitin sulfate support the development of mouse oocyte fertilized in vitro to the blastocyst stage. Furthermore, these results suggest that glycosaminoglycans can be utilized to support embryo development in vitro as a nutrient instead of serum.

• Journal of Veterinary Clinics
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• v.38 no.2
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• pp.56-62
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• 2021

Madecassoside, an active ingredient extracted from Centella asiatica, is used for treatment of various skin disorders in humans. However, the effect of madecassoside on the skin of dogs and cats has not been studied yet. The purpose of this study was to evaluate clinical efficacy of topical madecassoside cream in dogs and cats with skin diseases. A total of twenty-one dogs and ten cats with various skin diseases were included in the study. The 1% topical madecassoside cream was applied to the animal's skin lesion at least once a day for 7 days, and the skin condition was evaluated before the application of madecassoside cream (day 0) and 7 days after the application (day 7). The skin condition was scored by five clinical indices: canine atopic dermatitis extent and severity index-4 (CADESI-4), coat condition, pruritus, scale, and general condition. In dogs, all five clinical indices (CADESI-4, coat condition, pruritus, scale, and general condition) were significantly decreased on day 7 compared to those on day 0 (p < 0.0001, p < 0.05, p < 0.001, p < 0.01, and p < 0.05, respectively). In cats, the CADESI-4 and scale were significantly decreased on day 7 compared to those on day 0 (p < 0.01 and p < 0.05, respectively). No adverse effects were observed during the trial period in the dogs and cats included in this study. The results of this study demonstrate that the topical madecassoside cream is applicable to skin lesions in dogs and cats.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.3
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• pp.358-363
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• 2008

The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species such as pigs whose ooplasm is opaque and pronuclei are often invisible. In this study, the effects of sperm membrane disruption and electrical activation of oocytes on in vitro development and expression of transgene green fluorescent protein (GFP) in ICSI embryos were tested to refine this recently developed procedure. Prior to ICSI, sperm heads were treated with Triton X-100+NaCl or Triton X-100+NaCl+NaOH, to disrupt membrane to be permeable to exogenous DNA, and incubated with linearized pEGFP-N1 vector. To induce activation of oocytes, a single DC pulse of 1.3 kV/cm was applied to oocytes for $30{\mu}sec$. After ICSI was performed with the aid of a micromanipulator, in vitro development of embryos and GFP expression were monitored. The chemical treatment to disrupt sperm membrane did not affect the developmental competence of embryos. 40 to 60% of oocytes were cleaved after injection of sperm heads with disrupted membrane, whereas 48.6% (34/70) were cleaved without chemical treatment. Regardless of electrical stimulation to induce activation, oocytes were cleaved after ICSI, reflecting that, despite sperm membrane disruption, the perinuclear soluble sperm factor known to mediate oocyte activation remained intact. After development to the 4-cell stage, 11.8 (2/17, Triton X-100+NaCl+NaOH) to 58.8% (10/17, Triton X-100+NaCl) of embryos expressed GFP. The expression of GFP beyond the stage of embryonic genome activation (4-cell stage in the pig) indicates that the exogenous DNA might have been integrated into the porcine genome. When sperm heads were co-incubated with exogenous DNA following the treatment of Triton X-100+NaCl, GFP expression was observed in high percentage (58.8%) of embryos, suggesting that transgenic pigs may efficiently be produced using ICSI.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.515-524
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• 2020

Objective: Human mesenchymal stromal cells (MSCs) exhibit variable differentiation potential and can be divided accordingly into distinct subpopulations whose ratios vary with donor age. However, it is unknown whether the same is true in pigs. This study investigated MSC subpopulations in miniature pig and compared their characteristics in young (2 to 3 months) and adult (27 to 35 months) pigs. Methods: Osteogenic, chondrogenic, and adipogenic capacity of isolated MSCs was evaluated by von Kossa, Alcian blue, and oil red O staining, respectively. Cell surface antigen expression was determined by flow cytometry. Proliferative capacity was assessed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Expression of marker genes was detected by quantitative real-time polymerase chain reaction. Results: Porcine MSCs comprised cells with trilineage and bilineage differentiation potential (tMSCs and bMSCs, respectively) and non-differentiating stromal cells (NDSCs). The tMSC and bMSC fractions were smaller in adult than in young pigs (63.0% vs 71.2% and 11.6% vs 24.0%, respectively, p<0.05); NDSCs showed the opposite trend (25.4% vs 4.8%; p<0.05). Subpopulations showed no differences in morphology, cell surface antigen expression, or proliferative capacity, but octamer-binding transcription factor 4 (OCT4) expression was higher in tMSCs than in bMSCs and NDSCs (p<0.05), whereas sex determining region Y-box 2 (SOX2) expression was higher in tMSCs and bMSCs than in NDSCs (p<0.05). Aging had no effect on these trends. Conclusion: Porcine MSCs comprise distinct subpopulations that differ in their differentiation potential and OCT4 and SOX2 expression. Aging does not affect the characteristics of each subpopulation but alters their ratios.

• Journal of Sasang Constitutional Medicine
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• v.21 no.3
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• pp.30-38
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• 2009

1. Objectives: According to Sasang Constitutional Medicine, human beings are classified into four constitutions. Furthermore, medication and food should be taken differently based on the constitution because Sasang Constitutional Medicine is a field where all living things are perceived from a relative viewpoint instead of a standardized one. If so, what about the animals and its meat? This paper aims to identify the characteristics of the mics consumed, sonsially targeting pork and chicken as they are some of the most frequently enjoyed ones with the perception of the animals by the Sasang Constitutional Medicine. 2. Methods: We examine the differences between pigs and chickens through the behavior and nature of them such as morphology, sound, body temperature, taste etc and support the observed differences with medical application. 3. Results: From the Sasang Constitutional Medicine's perceptive, pig is considered as Soeumche while chicken is belongs to Soyangche. Thus, pork is recommended for the SY type while chicken is preferable for the SE type. 4. Conclusions: In this paper, we are trying to suggest several categories for the evaluation of the constitution in animal, especially for pig and chicken. We hope that this is one of the framework for the understanding the animal with Sasang Constitutional Medicine's viewpoint. And also, it may useful to apply the basis what we have outlined in this paper for improving the method of raising livestock as well as the research of laboratory animals.

• Journal of Veterinary Clinics
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• v.30 no.4
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• pp.288-291
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• 2013

The purpose of the retrospectivestudy was to describe the clinical findings and treatment of intracorneal hemorrhage (ICH) in canine small breeds. The medical records of 6 dogs with ICH (January 2007 to November 2011) were examined to obtain the breed, age, gender, affected eye, cornea area, treatment, and follow-up. A total of 12 corneal areas in 8 eyes were identified in 6 dogs (4 Yorkshire terriers, 1 Poodle, and 1 Maltese). The mean age ${\pm}SD$ at the first presentation was $12.3{\pm}2.8$years, and 5 of 6 dogs were over 12 years old. ICH caused by corneal neovascularization was the most common in the nasal area of cornea (nasal 6/12, superior 4/12, and inferior 2/12) and recurred in different area of same or other cornea in 3 dogs. There were no concomitant corneal diseases at the initial presentation. All dogs affected were treated with combination of topical antibiotics and steroids and subconjuctival injection of steroids. The results showed that ICH can be treated with long-term medical therapies. In addition, further study would be needed to identify specific causes for the intracorneal hemorrhage.

• Korean Journal of Veterinary Research
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• v.39 no.4
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• pp.730-737
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• 1999

A national animal health monitoring system(NAHMS) in Gyeongnam area was started from 1997 to develop statistically valid data for use in estimating disease frequencies in dairy cattle, and the associated costs. The objectives of this study were to : (1) discribe what was done to implement and maintain the system in Gyeongnam ; (2) present selected disease frequencies ; (3) discuss the epidemiological consideration of what was done and implications for the results obtained. Veterinary Medical Officers(VMOs-professors and graduate students from Gyeongsang National University, Faculty of Gyeongnam Livestock Promotion Institute, and Clinic veterinarians) served as data collectors. After training on current disease and management problems of dairy cattle, interview techniques, sampling methods, and data collection instruments, the VMOs participated in selection of the sample herds and data gathering. Forty of 167 dairy herds were selected randomly and the VMOs visited farms once a month for 12 months to collect data about management, disease, inventory, production, preventive treatment, financial and any other relevant data. Strict data quality control devices were used. Specific feed-back was developed for the producers and data collectors. Of the three age groups studied, cows had the greatest number of disease problems. The six disorders found most frequently were (from the highest to the lowest) breeding problems, clinical mastitis, birth problems, gastrointestinal problems. metabolic problems, and lameness. In young stock, respiratory, multiple system, breeding problems, and gastrointestinal problems were pre dominant, whereas in calves, gastrointestinal, respiratory, and integumental problems were major.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.280-283
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• 2007

A 6-year-old male dog was referred to Animal Medical Center, Chonbuk National University with large distal extremity abrasion wound to the hind limb by automobile accident. The wound was reconstructed with an autogenous, full-thickness skin mesh graft. The graft was harvested from the ventrolateral abdominal wall and transplanted to the wound. The skin mesh graft survived successfully without any noticeable complications. Successful grafting requires asepsis, an adequately prepared recipient bed through the surgical debridement and lavage, proper harvesting and preparation of the graft. Full-thickness skin mesh graft has lesser skin contraction which prevents formation of scar. Meshing the graft provides more graft flexibility over uneven, convex and concave surfaces and allows adequate drainage. The full-thickness skin mesh graft can be successfully used for the treatment of large distal skin wounds in dogs.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.276-279
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• 2007

A 2-year-old male, 3 kg body weight Japanese Chin was injured in the automobile accident three months ago. The dog became antebrachiocarpal joint instability, and performed pancarpal arthrodesis using 3 K-wires in localanimal hospital. But, the result was failure. Therefore the dog was referred to Chonbuk Animal Medical Center, Chonbuk National University. In physical examination, right carpal joint instability, knuckling sign and pain were evident. In radiography, sclerosis was observed on the 4th carpal bone. Complete blood count (CBC), serum chemistry and urinalysis finding were within reference ranges. Pancarpal arthrodesis was re-performed using 7-hole plate. However, mild skin and muscle defects was appeared by skin tension of extremity. We expected that granulation would fill the defect, but inflammation was continued on the lesions for 3 days. So, operation which is filling it was done by using the muscle flap and tubed skin flap. The donor muscles were flexor carpi radialis and superficial digital flexor muscles. After 7 days, the muscle flap was survived, but tubed skin flap was necrosed. After 20 days, the skin defect was substituted with granulation tissues. The flexor carpi radialis muscle and superficial digital flexor muscle transposition can be a useful procedure for reconstructing soft tissue defects in the carpal and metacarpal areas.