• Journal of Life Science
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• v.12 no.3
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• pp.264-273
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• 2002

We have isolated and artificially expressed three cDNA clones of Capsicum annuum PR5 genes for elucidating the antifungal activity against Phytophthora capsici which contracted a hot pepper root rot in field condition. Three divergent PR5 proteins from hot pepper were designated as CAPR5-1 and CAPR5-2 from susceptible cultivar (Subicho) as well as CAPR5-3 from resistant cultivar (CM331) in response to P. capsici. The cDNA similarity was found over 80% of identity among the three CAPR5s, and deduced amino acid sequence was characterized that all of CAPR5s contained 16 cysteine residues which possibly had a significant role in the structural formation. The result of genomic DNA blot showed that CAPR5-1 and CAPR5-2 existed as single copy in the Subicho genome. Three recombinant CPARs in E. coli were identified by SDS-PACE, and each expressed protein was treated on the PDA medium which contained cultured pathogens. Although three CAPR5 proteins did not affected the hyphal growth of Glomerella glycines and Colletotrichum fagenarium, CAPR5-1, CAPR5-2, and CAPR5-3 showed a specific antifungal activities against P. capsici.

• Journal of Bio-Environment Control
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• v.28 no.3
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• pp.225-233
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• 2019

In the summer season, natural ventilation is commonly used to reduce the inside air temperature of greenhouse when it rises above the optimal level. The greenhouse shape, vent design, and position play a critical role in the effectiveness of natural ventilation. In this study, computational fluid dynamics (CFD) was employed to investigate the effect of different roof vent designs along with side vents on the buoyancy-driven natural ventilation. The boussinesq hypothesis was used to simulate the buoyancy effect to the whole computational domain. RNG K-epsilon turbulence model was utilized, and a discrete originates (DO) radiation model was used with solar ray tracing to simulate the effect of solar radiation. The CFD model was validated using the experimentally obtained greenhouse internal temperature, and the experimental and computed results agreed well. Furthermore, this model was adopted to compare the internal greenhouse air temperature and ventilation rate for seven different roof vent designs. The results revealed that the inside-to-outside air temperature differences of the greenhouse varied from 3.2 to $9.6^{\circ}C$ depending on the different studied roof vent types. Moreover, the ventilation rate was within the range from 0.33 to $0.49min^{-1}$. Our findings show that the conical type roof ventilation has minimum inside-to-outside air temperature difference of $3.2^{\circ}C$ and a maximum ventilation rate of $0.49min^{-1}$.

• 한국노년학
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• v.40 no.2
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• pp.269-289
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• 2020

The purpose of this study was to analyze the policy and programs of the Seoul50Plus Foundation and to propose policies and programs to promote active aging of middle-aged and elderly people. A single case study was utilized as a research method. The analysis framework of case was approached with four frameworks: the context of policy and program, ideology-goal analysis of policy and program, supplier and customer analysis of policy and program, and process and domain analysis of policy and program. The analysis data included the homepage content of the Seoul50Plus Foundation, related 50+ policy and program data, active aging research papers at home and abroad, and presentation and meeting materials. The results of the research showed that the policy and program background and environment were essential elements for responding to environmental changes in the aging society. In terms of the philosophy-goal, job creation, social participation, and approach to life design were systematically carried out for the ideology of 'creating a new life vision' and promoting active aging. In terms of subject-target, the subject of policy implementation is systematically composed of three stages: 50+ foundation, 50+ campus, and 50+ center. The target was limited to 50+ generations(age 50~64) and had a problem that is limited to applicants. In the process-area analysis, programs such as educational support, work and entrepreneurship support, counseling information, and new-age cultural creation activities were designed to increase added value according to the characteristics of the middle-aged people. In conclusion, the future Seoul50Plus Foundation's policy and lifelong learning programs need to be systematically promoted through cooperation with businesses and universities, expanding the target audience, matching the characteristics of the middle-aged and elderly, and linkage among programs.

• Journal of Korea Entertainment Industry Association
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• v.14 no.4
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• pp.67-75
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• 2020

Metonymy can be said to be the only language's meaning shifting technique that exists in the domain of a single human thought in order to obtain a transparent cognitive effect. The purpose of this study was to analyze the 'descriptive metonymy' of the advertising content language constructed by the cognitive principle and to find a way to use it in media language education for social and cultural interests and reflection of college students. The metonymy used in advertising media contrasts with the difficulty of the metaphorical interpretation of "opaque and distant" reasoning. Storyboards, mostly focused on human emotions and behaviors, used metonymy's 'transparent and easy meaning shifting technique'. I have found that I can expect the efficiency of media language education that contains the interest and sociocultural interest, self-reflection, and future imagination of college students. Now, there is less need to perform cognitive reasoning for advertisements with ambiguous metaphor techniques. Lastly, in order to produce successful advertising content, we expect to use the language technique of 'narrative metonymy' with warm feelings of humans, and acknowledge the lack of quantitative research and leave it as a task for the next research.

• Therapeutic Science for Rehabilitation
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• v.11 no.4
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• pp.7-22
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• 2022

Objective : This study aims to provide clinical evidence by systematically reviewing domestic CO-OP intervention studies. Methods : 13 papers suitable for selection criteria were finally selected and conducted. The analysis was divided into general characteristics and qualitative levels. Results : As a result of analyzing the contents of study, research has been conducted continuously since 2015, and the level of evidence for CO-OP intervention study was mainly single subject design. The subjects of the study were applied to both children and adults, and as the target activity areas of CO-OP intervention, children were shown to be play and leisure, and adults were instrumental daily living. COPM/PQRS were frequently used for evaluation to examine effectiveness of interventions. For domain specific strategies, children used body position, attention to doing, task specification, and verbal motor mnemonic. For adults, body position, attention to doing, task specification, and feeling to movement, verbal motor mnemonic, verbal rote script were used. Conclusion : It was found that CO-OP intervention is continuously being applied in occupational therapy, and age and diagnosis group are expanding. It is significant in that it provided evidence for implementing CO-OP interventions in clinical practice through a systematic review of domestic CO-OP intervention studies.

• The Journal of Korean Academy of Sensory Integration
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• v.20 no.3
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• pp.72-85
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• 2022

Objective : This study was conducted to examine self-selected goals and the outcome measures used in the Cognitive Orientation to daily Occupational Performance (CO-OP) approach for Developmental Coordination Disorder. Methods : Studies published from January 2012 to October 2022 in the PubMed, Embase, ScienceDirect, Cochrance Library databases were searched. Keywords used for search were ('developmental coordination disorder' OR 'DCD') AND ('Cognitive Orientation to daily Occupational Performance' OR 'Cognitive Orientation to Occupational Performance' OR 'CO-OP'). Among 211 searched studies, 7 selected studies that match the thesis of this study were analyzed. Results : The selected studies showed a relatively high level of evidence overall, including two randomized experimental studies, one non-random two-group study, three non-random one-group studies, one single-subject study. The self-selected goals preference of the children was high in the order of play, education, and daily life activities. Most of applicable sessions were conducted 10 times during a 1-h period, and intervention effects showed positive outcomes on the occupation performance motor domain. To measure the effectiveness of CO-OP, the improvement of occupational performance was evaluated using Canadian Occupational Performance Measure (COPM) and Performance Quality Rating Scale (PQRS), and the improvement of motor skills was evaluated using Movement Assessment Battery for Children (M-ABC). Conclusion : This study is expected to be used as basic clinical data when applying the CO-OP approach to Developmental Coordination Disorder.

• Journal of Practical Engineering Education
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• v.14 no.3
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• pp.505-512
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• 2022

The purpose of this study is to synthesize effects of the living lab-based education through meta-analysis. Seven primary studies reporting the effect of living lab-based education were carefully selected for data analysis. Research questions are as follows. First, what is the overall effect size of the living lab-based education? The overall effect size refers to the effect on the cognitive and affective domains. Second, what is the effect size of the living lab-based education according to categorical variables? Categorical variables are outcome characteristics, study characteristics, and design characteristics. Results are summarized as follows. First, the overall effect size of living lab-based education was 0.347. Second, the effect size according to the cognitive domain was 1.244 for information process, 0.593 for communication, 0.261 for problem solving, and 0.26 for creativity. Third, the effect size according to subject area was shown in the order of electrical and electronic engineering 1.146, technology and home economics 0.489, artificial intelligence 0.379, and practical arts 0.168. Fourth, the effect size according to school level was 1.058 for high school, 0.312 for middle school, and 0.217 for elementary school. Fifth, the effect size by grade level was 0.295 when two or more grades were integrated and 0.294 for a single grade.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.19 no.3
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• pp.173-186
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• 2024

This paper analyzes a case of successful faculty entrepreneurship through a coupled process of open innovation in a university context, using the core competency theory perspective. Initially, the current state of faculty entrepreneurship is examined, and the effects of interdisciplinary coupled processes of open innovation are explored, focusing on the case of 'Omotion Inc.,' a startup utilizing generative AI technology for hyper-realistic 3D virtual human experiences. The research methodology involves in-depth interviews with Omotion Inc.'s co-founders, technology commercialization professionals, and experts in the field, followed by analysis based on foundational theories. Applying the core competency theory, this paper scrutinizes the process of integrating diverse expertise and technologies from various academic disciplines. The analysis goes beyond the limitations of faculty entrepreneurship confined to a single technology-centric research domain. Instead, it explores the possibilities of enhancement and value creation through coupled processes, providing practical implications for the university entrepreneurial ecosystem. The aim is to extend the traditional roles of education and research within the university, presenting a role in economic value creation beyond the boundaries of conventional faculty entrepreneurship. Through the collaboration of two faculty members, this study showcases the creation of novel technology and business models. It establishes that successful coupled processes of open innovation in faculty entrepreneurship, from a core competency theory perspective, require the entrepreneurial firm to possess (1) entrepreneurial capabilities, (2) technological capabilities, and (3) networking capabilities. The implications of this research highlight the positive impact of coupled processes of open innovation in faculty entrepreneurship, as evidenced by the Omotion Inc. case, offering guidance on entrepreneurial directions for university members preparing for entrepreneurship.

• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.177-183
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• 2008

Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless(Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626(NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2(C3-H2-C3 type) domain for this group of protein is $Cys-X_2-Cys-X_{28}-Cys-X-His-X_2-His-X_2-Cys-X_{14}-Cys-X_2-Cys$. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.

• Childhood Kidney Diseases
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• v.3 no.2
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• pp.209-216
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• 1999

Purpose : Nephrogenic diabetes insipidus (NDI) is a rare X-linked disorder associated with renal tubule resistance to arginine vasopressin (AVP). The hypothesis that the defect underlying NDI might be a dysfunctional renal AVPR2 has recently been proven by the identification of mutations in the AVPR2 gene in NDT patients. To investigate the association of mutations in th AVPR2 gene with NDI, we analyzed the AVPR2 gene located on the X chromosome. Methods : We have analyzed the AVPR2 gene in a kindred with X-linked NDI. The proband and proband's mother were analyzed by polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP) and DNA sequencing of the AVPR2 gene. We also have used restriction enzyme analysis of genomic PCR product to evaluate the AVPR2 gene. Results : C to T transition at codon 202, predictive of an exchange of tryptophan 202 by cysteine(R202C) in the third extracellular domain was identified. This mutation causes a loss of Hae III site within the gene. Conclusion : We found a R202C missense mutation in the AVPR2 gene causing X-linked NDI, and now direct mutational analysis is available for carrier screening and early diagnosis.