• The Journal of the Korean Society for Microbiology
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• v.22 no.3
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• pp.259-266
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• 1987

A total of 129 clinical isolates of Staphylococcus species was characterized by the tests of coagulase production, haemagglutination, mannitol fermentation, DNase production and hemolysis. Ninety-nine out of them showed positive reactions to the tests, therefore they were identified as Staphylococcus aureus. The isolates showing positive reaction in haemagglutination test also showed 100% of tube coagulase positive reaction. The haemagglutination test was a reliable method for identifying Staphylococcus aureus in the clinical laboratory. S. aureus produced stronger hemolysis with human blood agar than with sheep blood agar. Antibiotic resistant S. aureus isolates(S-46, S-112, S-126) had 4 to 6 p]asmid DNA elements. The S-112 strain had 6 plasmid DNA elements(1.8, 2.2, 3.7, $26.3{\sim}50$, and 70 Mdaltons), the S-126 had 4 elements(2.6, 4.2, $4.6{\sim}60Md$), and the S-46 had 1 element(${\sim}100Md$). PPSA strain had 4 plasmid DNA elements(2.5, 4.2, $4.6{\sim}60Md$) and S. aureurs(ATCC) strain contained 9.4, 26.3 and ${\sim}50Md$ plasmid DNA elements.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.216-220
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• 2002

To obtain the basic information for the utilization of Salicornia herbacea L. as a raw material in food and chinese herbs, chemical components of it were investigated. Leaves and stems of S. herbacea L. from the western coast of Korea were used after dry and grinding with powder. Dietary fiber, total sugar and uronic acid contents of S. herbacea L. were 60.7, 15.2 and 2.6% , respectively. This result suggest that dietary fiber content of S. herbacea L. is so higher than others. Choline and betaine which are important as bioactive compound in body were detected with the value of 29mg/100g and 888mg/100g, respectively. Mineral components of S. herbacea L. were rich in Na (2880mg/100g), Ca (440mg/100g), K (930mg/100g) and Mg (356mg/100g).

• Journal of Environmental Science International
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• v.16 no.6
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• pp.763-769
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• 2007

This study compared the performance of a bioscrubber, a biofilter, and a combined system of bioscrubber and biofilter employed being operated at the laboratory-scale. for the removal of hydrogen sulfide. The bioscrubber maintained 100% removal of hydrogen sulfide up to inlet load of $56\;g-S/m^3{\cdot}hr$, while the removal efficiency was decreased with the increase of inlet load. The biofilter showed 100% removal efficiency up to inlet load of $126\;g-S/m^3{\cdot}h$ and the maximum elimination capacity of $126\;g\;S/m^3{\cdot}h$ for the inlet load of $224\;g-S/m^3{\cdot}h$. On the other hand, the combined system of bioscrubber and biofilter showed 100% removal for an inlet hydrogen sulfide load of up to $85\;g-S/m^3{\cdot}h$ and the maximum elimination capacity of $153\;g-S/m^3{\cdot}h$ for inlet loads of $224\;g-S/m^3{\cdot}h$.

• Korean Journal of Veterinary Research
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• v.21 no.2
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• pp.167-170
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• 1981

Changes in serum glutamic oxaloacetic transaminase (s-GOT) activities, serum alkaline phosphatase activities and serum total protein amounts were investigated on seven Korean native cows having normal estrus cycle of 18~24 days after normal parturition, dividing estrus cycle into estrus (0~1 days), metestrus (2~6 days), diestrus (7~16 days), proestrus (17~20 days). The results were as follows. 1. Serum GOT activities at estrus ranged from 73.5 to 121.5 Ku with a mean of $89.14{\pm}17.16$ Ku, at metestrus 57 to 89 Ku with a mean of $67.01{\pm}10.81$ Ku, at diestrus 54 to 89.5 Ku with a mean of $67.05{\pm}10.05$ Ku and at proestrus 53 to 112 Ku with a mean of $73.00{\pm}20.05$ Ku. The activities were significantly increased at the estrus comparing with other stages (P<0.01). 2. Serum ALP activities at estrus ranged from 8.0 to 10.4 K-Au with a mean of $8.74{\pm}0.83$ K-Au, at metestrus 6.5 to 9.2 K-Au with a mean of$7.74{\pm}0.76$ K-Au, at diestrus 5.6 to 9.0 K-Au with a mean of $7.67{\pm}1.13$ K-Au, at proestrus 6.2 to 9.3 K-Au with a mean of $7.12{\pm}1.18$ K-Au. The significance was not recognized among the stages of estrus cycle. 3. Serum total protein amounts at estrus ranged from 6.45 to 8.0g/10dl with a mean of $7.25{\pm}0.57/100dl$, at metestrus, 6.37 to 7.9g/100dl with a mean of $7.65{\pm}0.50g/100dl$, fat diestrus, 6.56 to 8.67g/100dl, with a mean of $7.53{\pm}0.55g/100dl$ and at proestrus 5.94 to 7.71g/100dl with a mean of $6.54{\pm}0.65g/dl$. There was not significance among the stages of estrus cycle.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.12
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• pp.1865-1870
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• 2014

To synthesize ${\omega}$-3 and ${\omega}$-6 balanced polyunsaturated fatty acid (PUFA) oil mixture, flaxseed oil and sesame oil were mixed and their anti-oxidative and sensory characteristics analyzed. For the fatty acid composition analysis results, the ${\omega}$-3 and ${\omega}$-6 PUFA ratios of the F20S80 oil mixture (flaxseed oil 20% and sesame oil 80% oil mixture) and F10S90 (flaxseed oil 10% and sesame oil 90% oil mixture) were represented as 1 to 10 and 1 to 5 ratio for ${\omega}$-3 and ${\omega}$-6 PUFA content from 4.4% to 42.1% and 8.9% to 39.7%, respectively. Since these were within 1:4~1:10 for healthy functional ${\omega}$-3 and ${\omega}$-6 balanced PUFA oil mixtures, these oil mixtures were healthy functional oil mixtures. To analyze anti-oxidative effects, acid values were analyzed. Samples were stored at room temperature, 70% relative humidity (RH) and $45^{\circ}C$, and 70% RH for 16 weeks. The acid value between F10S90 and S100 stored at $45^{\circ}C$ and 70% RH for 16 weeks were not statistically significant. The sensory characteristics such as oxidative odor and sesame odor and taste were not statistically significant among F20S80, F10S90, and sesame S100. Sensory characteristics between F10S90 and S100 stored at $45^{\circ}C$ and 70% RH for 16 weeks were not statistically significant. In conclusion, the sensory and oxidative characteristics of F10S90 were similar to those of S100. Therefore, F10S90 oil mixture should be used as a ${\omega}$-3 and ${\omega}$-6 balanced PUFA healthy functional oil mixture with high anti-oxidative effects.

• Journal of Embryo Transfer
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• v.21 no.3
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• pp.233-239
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• 2006

This study was carried out to investigate the effect of intra-uterine infusion of lipopolysaccharides (LPS) on uterine involution in dairy cows with retained placenta. The LPS isolated from Bacteroides helcogenes and Fusobaoterium varium was infused at the rate of 100 ug (n=11) and 200 ug (n=11) with 30 ml of phosphate buffer saline in each cow at 20 days post-partum. The cows in conrol group (n=11) with retained placenta were infused with 30 ml phosphate buffer saline in each cow at 20 days postpartum. Cross-sectional ultrasonography was performed at days 40 postpartum to examine uterine involution and exudate in the all dairy cows in study. The cows in control group have shown 45.5, 27.3 and 27.3% gravid horn with less than 30 mm (normal), $31{\sim}50mm$ (medium) and more than 51 mm (large) in diameter. Respective average values of gravid horn diameter in cows treated with LPS 200 ug were noticed 72.7, 18.2 and 9.1%. However, the gravid horn diameter in all the cows treated with 100 ug of LPS was less than 30 mm. The 18.2, 63.6 and 54.6% cows in the control, LPS 100 ug and LPS 200 ug, respectively, have shown no exudate in uterine cavity. However, the respective rates of small amount of exudate retention in uterine cavity were 45.5, 0.0, and 9.1%. Approximately 36.4% cows in all 3 treated groups have shown minute quantity of exudate. In conclusion, the LPS intra-uterine infusion promoted postpartum uterine involution in dairy cows with retained placenta.

• Korean Journal of Veterinary Service
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• v.32 no.3
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• pp.227-239
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• 2009

At the present study, it was aimed to detect virulence genes and antimicrobial resistance genes among 102 strains of 12 Salmonella serotypes isolated from pigs and cattle. In polymerase chain reaction (PCR), invA was detected from all strains of Salmonella spp., spvC was detected from Salmonella enterica serotype Enteritidis (S. Enteritidis) (100%), S. Bradenburg (75%), and S. Typhimurium (20.4%). Drug resistance related genes of 12 types were detected from all strains. TEM ($bla_{TEM}$) gene was detected from 51 (92.7%) of 55 $\beta$-lactams (54 ampicillin or 1 amoxicillin) resistance strains. 55 (100%) of 55 chloramphenicol resistance strains, 3 (100%) of 3 gentamicin resistance strains and 5 (100%) of 5 kanamycin resistance strains did contain cml, aadB, and aphA1-Iab, respectively. strB (89.9%), strA (88.4%), aadA2 (84.1%) and aadA1 (72.5%) were detected from 69 streptomycin resistance strains. sulII and dhfrXII were detected from 49 (100%) of 49 sulfamethoxazole/trimethoprim resistance strains, but sulI was not detected. tetA (97.9%) and tetB (21.6%) were detected from 97 tetracycline resistance strains. int gene was detected from 58 (56.9%) of 102 strains. 54 S. Typhimurium of 102 Salmonella spp. were attempted to detect drug resistance genes. TEM was detected from 44 (95.7%) of 46 $\beta$-lactams (45 ampicillin or 1 amoxicillin) resistance strains. cmlA was detected from 51 (100%) of 51 chloramphenicol resistance strains. aadA2 (100%), strA (100%), strB (100%), and aadA1 (79.6%) were detected from 54 streptomycin resistance strains. sulII (100%) and dhfrXII (100%) were detected from 49 sulfamethoxazole/trimethoprim resistance strains. tetA was detected from 54 (100%) of 54 tetracycline resistance strains. int gene was detected from 54 (100%) of 54 strains. The major drug resistance pattern and resistance gene profile were ampicillin, chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACSSuT) and TEM, cmlA, aadA1, aadA2, strA, strB, sulII, dhfrXII, tetA and int, respectively.

• KSBB Journal
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• v.18 no.2
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• pp.100-106
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• 2003

An on-line frontal analysis HPLC system was developed to determine the unbound concentration of (S)-perillyl alcohol, an potential anti-cancer agent, in human serum albumin (HSA) solution, The analysis was performed on a Develosil 100 Diol 5 (10 cm x 4.6 mm I.D.) high-performance frontal analysis (HPFA) column. Sodium phosphate solution was used as the mobile phase (pH 7.4, ionic strength 0.17) at a flow rate of 1 $m\ell$/min. UV wavelength was set at 205 nm. A injection volume of 600${mu}ell$ was chosen to ensure the compound eluted formed a zonal peak with a plateau. By Scatchard analysis, it was found that the binding constant(K) and binding number(n) of (S)-perillyl alcohol to molecular HSA were 2.05 x $10^6$ [$M{-1}$], 0.00428, respectively.

• Education of Primary School Mathematics
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• v.13 no.2
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• pp.55-66
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• 2010

The basic direction of mathematical education for the 21st century is focused on helping student to understand mathematics and developing their problem solving abilities, mathematical disposition and mathematical thinking. Elementary mathematics teachers should help students make sense of mathematics, confident of their ability, and make learning environment comfortable for students to participate in. Through making activities 1 to 100 with digits 1,9,9,6, students improved the interest and preference of students about mathematics. This game is useful to foster students' mathematical thinking(concepts of exponential number expression, roots concept(${\sqrt}$), gauss function([])) and mathematical disposition. If students are helped to be interested in mathematics through mathematical games, they regard mathematics as interesting and challengeable subject to let themselves think many ways.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.2
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• pp.190-197
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• 2020

The effects of the number of frozen-thawed ram sperm per single and double intra-cervical artificial insemination (AI) on fertility in ewes were studied. A total of 89 non-pregnant ewes were synchronized for oestrus with two doses of 100 ㎍ PGF_2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; D200 (n = 23; double AI with 200 × 10⁶ sperm), S200 (n = 24; single AI with 200 × 10⁶ sperm), D100 (n = 24; double AI with 100 × 10⁶ sperm) and S100 (n = 18; single AI with 100 × 10⁶ sperm). Ewes were inseminated within 12 to 18 h for single AI and, within 10 to 12 h and 16 to 18 h for double AI after the onset of oestrus. The onset of oestrus ranged from 28 to 76 h (54.33 ± 1.28 h). The high percentage (29.2%) of ewes showed oestrus between 51 to 60 h. The non-return rates were highest in group D200 (56.5%) and differed significantly (p < 0.05) from group S100 (11.1%). No ewes were pregnant in group S100, and the pregnancy rates among the remaining groups did not differ. The mean gestation period was 152.8 ± 0.5 days and no difference was observed among the groups. The lambing and multiple birth rates were 100% in group D200. The single and twin lambing was highest in group D100 (33.3%) and group D200 (83.3%), respectively. Only one triplet lambing and the highest lambing size (2.2 ± 0.2) was recorded in group D200. In conclusion, double AI with 200 × 10⁶ sperm showed comparatively most practical for achieving high pregnancy rates and lambing performances in Bangladeshi ewes under field conditions.