• Journal of Microbiology and Biotechnology
• /
• v.25 no.7
• /
• pp.1056-1069
• /
• 2015

Osmotic pressure is a critical factor for erythritol production with osmophilic yeast. Protein expression patterns of an erythritol-producing yeast, Yarrowia lipolytica, were analyzed to identify differentially-expressed proteins in response to osmotic pressure. In order to analyze intracellular protein levels quantitatively, two-dimensional gel electrophoresis was performed to separate and visualize the differential expression of the intracellular proteins extracted from Y. lipolytica cultured under low (3.17 osmol/kg) and high (4.21 osmol/kg) osmotic pressures. Proteomic analyses allowed identification of 54 differentially-expressed proteins among the proteins distributed in the range of pI 3-10 and 14.4-97.4 kDa molecular mass between the osmotic stress conditions. Remarkably, the main proteins were involved in the pathway of energy, metabolism, cell rescue, and stress response. The expression of such enzymes related to protein and nucleotide biosynthesis was inhibited drastically, reflecting the growth arrest of Y. lipolytica under hyperosmotic stress. The improvement of erythritol production under high osmotic stress was due to the significant induction of a range of crucial enzymes related to polyols biosynthesis, such as transketolase and triosephosphate isomerase, and the osmotic stress responsive proteins like pyridoxine-4-dehydrogenase and the AKRs family. The polyols biosynthesis was really related to an osmotic response and a protection mechanism against hyperosmotic stress in Y. lipolytica. Additionally, the high osmotic stress could also induce other cell stress responses as with heat shock and oxidation stress responses, and these responsive proteins, such as the HSPs family, catalase T, and superoxide dismutase, also had drastically increased expression levels under hyperosmotic pressure.

• Journal of The Korean Association For Science Education
• /
• v.35 no.1
• /
• pp.121-130
• /
• 2015

Identifying students' achievement level and reflecting it on educational policy making or instructional improvement by analyzing the results of nationwide standardized assessment is an integral part of accountability in education. On the basis of this premise, we analyzed the characteristics of National Assessment of Educational Achievement (NAEA) items for middle school science subject conducted from 2010 to 2013 by using the option response rate distribution curve, the fittest graph estimated from the response rate of correct/incorrect options by achievement score. Furthermore, we classified the type of option response rate curve in terms of correct and incorrect options. Results of the analysis of option response curve showed that five types of correct option response curve (S-shaped, J-shaped, straight-shaped, F-shaped, and step-shaped) and 4 types of incorrect option response curve (down-slope, flat, mound, and up-slope) were identified. The most common type of items was the combination of S-shaped correct option response curve and down-slope incorrect option response curve, which are considered as appropriate items to discriminate the students according to achievement level. Moreover, correct option response was found to be correlated with incorrect option response. Based on the results, we also discussed some implications on teaching-learning method and classroom assessment in science education.

• Journal of the East Asian Society of Dietary Life
• /
• v.18 no.4
• /
• pp.608-617
• /
• 2008

This study examined the level of reaction towards damage related to health foods and the factors affecting this reaction among the elderly. Data were collected from 269 elderly individuals living in Seoul, Daejeon, Daegu, Gwangju and Busan. Frequencies, chi-square tests, and a multinomial logistic regression analysis were performed using the SPSS v. 14.0 program. When asked about their reaction towards damage related to health foods, approximately 48% of the respondents answered 'no response', 34% answered 'private response', and 18% answered 'public response'. Multinomial logistic regression analysis revealed that education level and awareness of health food price were significant factors influencing 'private response', and concerns about health foods and awareness of damage redemption were significant factors for 'public response'. These results imply that consumer education for elderly to prevent damage derived from the purchase and consumption of health foods is required.

• The Korean Journal of Community Living Science
• /
• v.24 no.2
• /
• pp.209-220
• /
• 2013

This study is based on the interviews with six mothers to analyze their verbal response types with their young children ages 3-5 attending day-care center. This study was done from 10AM. to 12PM. August 18th 2012 at the director's room at P day care center in Seoul. For this study the following four situations mothers experience in child-rearing have been selected: self-achievement, sibling quarrel, nagging and isolation. As for the interview questionnaires the four verbal response types-stimulating, problem-solving, critical and indifferent ones-have been used. According to the results of this research, mothers tend to use stimulating verbal response types like praise in the situation of self-achievement; problem-solving response types like command in the situation of sibling quarrel; problem-solving response types like warning in the situation of nagging; and lastly, stimulating verbal response types like empathy in the situation of isolation. Mothers need to form more empathy with their children in such difficult situations as sibling quarrel or nagging. For mothers' efficient verbal responses for their children, accordingly, empathy in parent education needs to be more emphasized.

• The Korean Journal of Emergency Medical Services
• /
• v.26 no.1
• /
• pp.21-36
• /
• 2022

Purpose: This descriptive research study aimed to investigate the knowledge and perception of the natural disaster medical system by relevant disaster medical response teams in Jeonnam region, and provide baseline data for a disaster education program based on analysis of priorities of educational demand. Methods: Online questionnaires were distributed to 200 research participants including paramedics from five fire stations in J province, 22 public health centers, two disaster base hospitals, ERU (Emergency Response Units), and DMAT (Disaster Medical Assistance Team). The questionnaires elicited basic information about respondents, their knowledge and perception on disaster preparation and response, cooperation system, and educational and training needs. Results: The top priority items selected were: other disasters for paramedics, first aid for the rapid response team, and command system for DMAT. Conclusion: Customized education and training programs must be developed to suit each organizational need. Detailed operational guidelines must be established and with them a unified educational curriculum should be put into practice.

• The Korean Journal of Emergency Medical Services
• /
• v.27 no.1
• /
• pp.19-30
• /
• 2023

Purpose: The study examined disaster recognition, disaster preparedness, and the willingness to participate in disaster response among healthcare students. It also identified the factors that can improve healthcare students' willingness to participate in disaster response. Methods: A structured questionnaire was designed, and data were collected from 171 students, who agreed to participate in the study at C-province between December 14, 2022, and December 28, 2022. Results: The scores of disaster preparedness and the willingness to participate in disaster response were 3.22 and 3.53, respectively. The willingness to participate in disaster response was high among those who had experienced a disaster and had disaster-related education. Furthermore, the willingness to participate in disaster response was significantly and positively correlated with disaster preparedness (r = 1.357, p<.001). Conclusion: It is important to mandate the provision of disaster preparedness education to increase the level and quality of disaster preparedness and the willingness to participate in disaster response.

• Journal of Fisheries and Marine Sciences Education
• /
• v.27 no.1
• /
• pp.1-12
• /
• 2015

This article starts with a review of the system characteristic(easiness, flexibility, reliability) and individual characteristic(self-efficacy, achievement motive, computer ability) through education satisfaction especially in relation to the education performance(perceived usefulness, educatee value). We found that all variables significantly affect the education satisfaction, but only computerized ability doesn't significantly affect the education satisfaction. Education satisfaction significantly affect the education performance. This paper suggests that the followings; first, response time of systems is valuable factor in KcLep. Second, self-efficacy and achievement motive are more important then computer ability in KcLep educatee. Finally, software developer and teacher always think about rapid response time of systems and exchange of data for the education performance in KcLep.

• Human Ecology Research
• /
• v.57 no.4
• /
• pp.535-544
• /
• 2019

This study analyzes the need for appropriate response education in schools for national health and sustainable development education in connection with particulate matter, which has recently emerged as a serious environmental and social problem. The survey was conducted on 1,200 teenagers(middle and high school students) in North Jeolla Province. The results of the study are as follows. First, the survey results were generally low in terms of environmental attitude and response(actuality) as well as the level of knowledge of particulate matter. Second, particulate matter-related education is rarely conducted at school sites; therefore, it is also necessary to develop an environmental education programs that can be a significant educational experience along with a specific educational orientation that is required to ensure the achievement of sustainable residential environment education. Finally, we hope that this study will help raise awareness on sustainable residential environment education amid difficulties in accessibility. Issues that need further study include: the absence of education materials, environmental education related to particulate matter, and guidance on how to maintain healthy living for individuals that help increase interest in a sustainable living environment for future generations.

• Korean Journal of Exercise Nutrition
• /
• v.23 no.1
• /
• pp.1-6
• /
• 2019

[Purpose] To date, there have been no studies on the response of wrist tremor to increased muscle activity during exercise. This study aimed to evaluate the wrist tremor response with increasing muscle activity during bench press exercise. [Methods] Triceps muscle activity and wrist tremor response were measured by electromyography and an accelerometer, respectively, during bench press exercise in 11 healthy men without weight-training experience. Subjects performed bench press at 30% repetition maximum (RM), and the rating of perceived exertion (RPE) and lactate concentration were measured before and after exercise. One week later, an equivalent number of bench presses at 30% RM was performed without weight load as a control trial (CT). [Results] RPEs and lactate concentrations significantly increased after resistance exercise (30% RM) from 7.4 to 14.3 and 1.7 to 4.9, respectively (P<.01), but no such difference was observed in the CT. Muscle activity linearly increased during the 30% RM exercise, and wrist tremors were shown to linearly decrease. A strong negative correlation was observed between the two variables (r=-0.88, P<.001). [Conclusion] We found that wrist tremors during resistance exercise, as measured using an accelerometer, can be used to predict muscle activity.

• Journal of The Korean Association of Information Education
• /
• v.5 no.2
• /
• pp.268-278
• /
• 2001

This study is design and implementation of diagnosis module using item response theory to assess level of student's knowledge in web-based instruction systems. Item response theory uses responses to items on a test or survey questionnaire to simultaneously locate both the items on the latent trait defined by the set of items while simultaneously scaling each item on the very same dimension. Existing method of measurement in web-based instruction system provided dichromatic learning after to be assess just with the total scores of exam. This measurement has an error that do not consider the level of student's knowledge. Moreover, this method can't perform an exact diagnosis of student knowledge and make student modeling to construct intelligent tutoring system. In this study, we present that design and implement a diagnosis module using item response theory to assess level of student's knowledge in web-based instruction systems