• Journal of Embryo Transfer
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• v.23 no.4
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• pp.245-250
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• 2008

This study was designed to investigate the effect of essential amino acids (EAA) and/or non-essential amino acids (NEAA) on the development of parthenogenetic and somatic cell nuclear transfer (SCNT) porcine embryos in vitro. To evaluate the timing of amino acids supplementation, activated oocytes were cultured in NCSU23-PVA with EAA, NEAA or NEAA+EAA (AAs) during specific periods as below: EAA, NEAA or AAs were supplemented during Day 0 to 6 (whole culture period: ALL), Day 2 to Day 6 (post-maternal embryonic transition period: POST-MET), Day 5 to Day 6 (post-compaction period: POST-CMP), Day 0 to Day 2 (pre-maternal embryonic transition period: PRE-MET), or Day 0 to Day 4 (post-compaction period: PRE-CMP). Supplementation of NEAA decreased cleavage rates in PRE-MET and PRE-CMP and also decreased blastocyst rates in POST-CMP. On the other hand, EAA significantly enhanced blastocyst formation rate in POST-MET and no detrimental effect on embryonic development in other groups. Interestingly, NEAA and EAA had synergistic effect when they were supplemented to the medium during whole culture period. Supplementation of AAs also enhanced SCNT porcine embryo development whereas BSA-free medium without AAs could not supported blastocyst formation of SCNT embryos. In conclusion, existence of EAA and NEAA in defined culture medium variously influences the development of parthenogenetic and SCNT porcine embryos, and their positive effect are only occurred when both EAA and NEAA are supplemented to the medium during whole culture period. Additionally, AAs supplementation enhances the blastocyst formation of SCNT porcine embryos when they are cultured in the defined condition.

• The Korean Journal of Malacology
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• v.30 no.3
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• pp.273-279
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• 2014

The mass mortalities have been occurring of Korean scallop Patinopecten yessoensis from 1997's to now in Korea east sea. Cages behavior and interference effect (common name; curtain effect) between scallop cages were investigated in culture grounds on the eastern coastal waters of Korea for understand to mechanism of rising about mass mortalities of Korean scallop quickly. The first experiment was carried out in circulating water channel to assess inclination angel from relationship between velocity and cages interval, velocity with culture cages position. An angle of inclination of scallop culture cages were 94.6 to 92.3 degree under a several velocity which were from 0.1 m/s and 131.9 to 118.1 degree under 0.5 m/s with cages interval were 1 m, 94.3 to 91.0 degree under velocity is 0.1 m/s and 133.2 to 122.4 degree under 0.5 m/s with cages interval were 1.5 m and 94.6 to 96.4 degree under velocity is 0.1 m/s and 131.7 to 131.8 under 0.5 m/s with cages interval were 2 m. The second experiment was designed to prove the tank test. Velocities were measured inside and outside of the scallop culture ground at eastern sea of Korea. The velocity of inside of the culture was the slowest as 0.1m/s. In this result, interference between former cage and after cage was occurred.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.285-290
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• 2008

This study was carried out to obtain an optimal liquid culture condition for Sarcodon aspratus mycelia. Among various basal culture media the GYS (glucoe-yeast extract-soytone) medium was the best for mycelial growth. The appropriate temperature was $25^{\circ}C$. Starch, maltose or glucose was excellent carbon sources for the mycelial culture, compared to others tested. As nitrogen nutrients, soytone and $NH_4-N$ were the best organic and inorganic nitrogen sources, respectively. Moreover, the optimal concentration of soytone was 3 g per one-liter medium. In addition, we also found that alanine, $(NH_4)H_2PO_4$, and nicotinic acid were the best aminoacid, phosphorus salt, and vitamin, respectively. When all optimal conditions described above were applied to culture medium, we were able to produce 5.7 g dry weight of S. aspratus mycelia per one-liter liquid medium within 20 days.

• Journal of Digital Convergence
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• v.19 no.12
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• pp.359-367
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• 2021

The purpose of this study is to analyze domestic patient safety culture research topics using text mining and CONCOR analysis. The research method was conducted in the stages of data collection, data preprocessing, text mining and social network analysis, and CONCOR analysis. A total of 136 articles were analyzed excluding papers that were not published. Data analysis was performed using Textom and UCINET programs. As a result of this study, TF (frequency) of patient safety culture-related studies showed that patient safety was the highest, and TF-IDF (importance in documents) was highest in nursing. As a result of the CONCOR analysis, a total of seven clusters were derived: knowledge and attitude, communication, medical service, team, work environment, structure, organization and management that constitute the patient safety culture. In the future, it is necessary to conduct research on the relationship between the establishment of a patient safety culture and patient outcomes.

• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1423-1432
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• 2021

To elucidate the role and mechanism of microbes, we combined culture-dependent and culture-independent approaches to investigate differences in gut bacterial composition between sows and weaned pigs. Under anaerobic conditions, several nonselective and selective media were used for isolation from fecal samples. All isolated bacteria were identified and classified through 16S rRNA sequencing, and the microbiota composition of the fecal samples was analyzed by metagenomics using next generation sequencing (NGS) technology. A total of 278 and 149 colonies were acquired from the sow and weaned pig fecal samples, respectively. Culturomics analysis revealed that diverse bacterial genus and species belonged to Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes were isolated from sow and weaned pigs. When comparing culture-dependent and culture-independent analyses, 191 bacterial species and 2 archaeal bacterial species were detected through culture-independent analysis, and a total of 23 bacteria were isolated through a culture-dependent approach, of which 65% were not detected by metagenomics. In conclusion, culturomics and metagenomics should be properly combined to fully understand the intestinal microbiota, and livestock-derived microbial resources should be informed by culturomic approaches to understand and utilize the mechanism of host-microbe interactions.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.105.2-106
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• 2000

No Abstract, See Full Text

• Archives of design research
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• v.13 no.3
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• pp.201-210
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• 2000

A current of culture changes constantly. The changes in culture have been taken the lead in by new generations. Now, we are connected with a new trend of culture led by new generation. Moreover, it is the younger class from teens to mid-twenties, that is, the new generation group that plays a major part in the new culture. They, in economic affluence, are accustomed to a particular consumption-oriented purchasing pattern in their own way while the old generations had to tighten their belts. A business cannot help getting fascinated with their potential consuming power. The new generation has established itself as a powerful individual consumer class, and is presenting more and more explosive and destructive purchasing pattern in today's consumption market where turmoil of IMF is at the end and 21st century started. As of now, it cannot be emphasized too much to research their consuming culture and tendency. It is suggested that existing designers work out a marketing strategy based on a close investigation of their disposition and cultural characteristics and go on looking into what the properties of a new coming generation's culture vary every time generations change in order to take them to a future consumer group.

• Convergence Security Journal
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• v.20 no.2
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• pp.3-13
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• 2020

Organizations are facing a new, diverse security threat as ICT based industrial environment arises. As a way of effective countermeasure for security threat, organizations are making an effort to establish internalization of security culture, targeting a organizational members. However, members' awareness toward security receptiveness is low as inconvenience exists in business process and existing security culture focuses on controlling and regulating. Accordingly, this research desires to develop a participatory security culture which can higher the efficiency of security work process and induce members' voluntary participation. A comparative analysis on security culture related prior researches is conducted and based on a drawn components, statistical verification is accomplished. It is expected to contribute on future research on measuring a security culture level.

• Journal of Engineering Education Research
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• v.11 no.1
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• pp.48-63
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• 2008

The engineering technical culture needs to be fostered since it has been an important factor that decides the results of engineering and affects the establishment of social culture to a great extent. However, it is considered that awareness about invisible environments required for the distribution, education, promotion, and development of the engineering culture are not sufficient enough even though physical environments such as investments in engineering and research facilities have reached a substantial level through a recent series of efforts. Thus, distributing cultural activities by improving social awareness is desperately required to solve these problems, To take realistic measures and establish social atmosphere are particularly important. In order to design strategies for the diffusion of the engineering technical culture in the position of users, this research suggests short-term and mid-term plans and operating strategies that could produce substantial results.

• Molecules and Cells
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• v.37 no.6
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• pp.473-479
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• 2014

Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation of feeder cells is needed at each subculture. In this study, we developed a Matrigel-based feeder-free culture system for long-term propagation of SSCs. Although several in vitro SSC culture systems without feeder cells have been previously described, our Matrigel-based feeder-free culture system is time- and cost-effective, and preserves self-renewability of SSCs. In addition, the growth rate of SSCs cultured using our newly developed system is equivalent to that in feeder cultures. We confirmed that the feeder-free cultured SSCs expressed germ cell markers both at the mRNA and protein levels. Furthermore, the functionality of feeder-free cultured SSCs was confirmed by their transplantation into germ cell-depleted mice. These results suggest that our newly developed feeder-free culture system provides a simple approach to maintaining SSCs in vitro and studying the basic biology of SSCs, including determination of their fate.