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An in vitro study of mesenchymal stem cell proliferation on titanium discs coated with rhTGF-β2/PLGA by electrospray (Electrospray법으로 rhTGF-β2/PLGA 복합체를 코팅한 티타늄에서의 간엽줄기세포 증식에 관한 연구)

  • Kim, Joohyung;Kim, Seong-Kyun;Heo, Seong-Joo;Koak, Jai-Young;Lee, Woo-Sung;Lee, Joo-Hee;Park, Ji-Man
    • The Journal of Korean Academy of Prosthodontics
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    • v.54 no.2
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    • pp.120-125
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    • 2016
  • Purpose: The purpose of this study is to identify the effect of mesenchymal stem cell proliferation on recombinant human transforming growth factor-beta (rhTGF-${\beta}2$) / poly (D,L-lactide-co-glycolide) (PLGA) treated titanium discs by electrospray. Materials and methods: Anodized titanium surface coated with PLGA was used for a control group to compare anodized titanium surface coated with 125 ng/ml and 500 ng/ml rhTGF-${\beta}2$ as test groups. Atomic force microscope (AFM) test was utilized to determine the difference in coating surface roughness, and field-emission scanning electron microscopy (FE-SEM) was taken to visualize even distribution of coating particles on titanium discs. The mesenchymal stem cell proliferation was tested by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl-tetrazolium bromide) assay on 1st, 4th, 7th days. Results: According to AFM results, there was no statistically significant difference in titanium discs treated with PLGA and with rhTGF-${\beta}2$/PLGA (P>.05). MTT assay test results showed that there was statistically significant difference in mesenchymal stem cell proliferation on test groups compared to control groups at 7th day, and cell viability on discs coated with rhTGF-${\beta}2$ was significantly higher than control groups (P<.05). Conclusion: Titanium surface coated with rhTGF-${\beta}2$/PLGA shows statistically significant higher cell proliferation and the titanium surface coated with the higher concentration of rhTGF-${\beta}2$ presents faster cell growth activity.

Physicochemical Quality Characteristics of Tomato Sauce Added with Pimpinella brachycarpa (참나물을 첨가한 토마토소스의 이화학적 품질특성)

  • Jang, Sang-Jun
    • Culinary science and hospitality research
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    • v.20 no.4
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    • pp.169-182
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    • 2014
  • This study investigates the physicochemical quality characteristics of tomato sauce added with different kinds of pimpinella brachycarpa. The results are as follows. The pH of tomato sauce added with freeze-dried pimpinella brachycarpa, in contrast to the tomato sauce with raw pimpinella brachycarpa, decreased as the addition quantity of the sample increased. As for sugar content, PBP0 with Pimpinella brachycarpa contained was shown to be the highest with 10.83. For chromaticity, all values (e.g, L, A and B) of tomato sauce added with pimpinella brachycarpa and with freeze-dried pimpinella brachycarpa decreased showing significant differences among the samples as the addition quantity of the samples increased. With increased addition quantity of the sample with freeze-dried Pimpinella brachycarpa, its water content decreased. PBP4, addition group with 4% of freeze-dried Pimpinella brachycarpa, was shown to be the highest DPPH free radical scavenging activity as 29.58. When adding Pimpinella brachycarpa to tomato sauce, a total number of micro-organisms decreased. In case of adding raw Pimpinella brachycarpa rather than freeze-dried Pimpinella brachycarpa, it created a further effect on an inhibitive action of growing and developing micro-organisms. In the preference of tomato sauce with Pimpinella brachycarpa, PBF2 with 2% of Pimpinella brachycarpa had the highest score as 5.1 for the color. For taste, 3.7, the lowest taste score, was shown in PBF1with 1% of Pimpinella brachycarpa. The overall preference revealed that PBF2 was the highest scoring 5.8.. PBF3 was scored highest with 5.9 for overall preference. From the comprehensive findings, 2~3% of the addition ratio between raw Pimpinella brachycarpa and freeze-dried Pimpinella brachycarpa in the production of tomato sauce is considered the most preferable.

A comparative study on perception of team teaching between vocational teachers and industry-educational adjunct teachers in Technical high school (팀티칭에 대한 공업계열 전문교과교사와 산학겸임교사 간 인식 비교 연구)

  • Son, Yeo-Ul;Lee, Byung-Wook
    • 대한공업교육학회지
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    • v.36 no.1
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    • pp.75-94
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    • 2011
  • The purpose of this study is to suggest the basic data in order to examine and perform the plan for activating the team teaching between industry-educational adjunct teachers and vocational teachers in technical high school. The research results are as follows. First, It is found that both teacher groups acknowledge the necessity of the team teaching, but vocational teachers are less likely to recognize the necessity than industry-educational adjunct teachers. Second, In the preparation of team teaching, both two groups of the teachers believe that the preliminary interchange and training between them are to be highly supportive for the activities expected to help teachers. Therefore, it is necessary to have opportunity of communication and narrow the difference of opinions between them by promoting the deep interest about applicable field and sharing the mutual idea between the teachers in the preparation of team teaching. Third, And the two groups recognize that the cooperation and joint establishment of design of team teaching and the individual process or joint progress of class activity are desirable for the proper design of team teaching. Therefore, it is necessary to establish the class environment for the interaction between teachers and students through not only the reciprocal activities between teachers but the interest class by systematically preparing the class design and role division clearly. Fourth, In the practice of team teaching, the two groups believe that the teaching activities can be usually divided and progressed, but it is desirable to work together in the related contents. The vocational teachers recognize that it is necessary to actively interact with students by connecting with the case of industry field. On the other side, industry-educational adjunct teachers think that the learning contents should be selected and organized according to the interests of students by associating with the case of industry field. Fifth, And two groups of teachers recognize that it is desirable to evaluate the grade by reflecting on the assessment by vocational teachers(50%), industry-educational adjunct teachers(50%).

Physiological Characteristics of Poplar Clones in the Saemanguem Reclaimed Land (새만금간척지에 식재한 포플러 클론의 생리적 특성)

  • Lee, Jong Kyu;Jang, Ji Hwi;Li, Yang;Kim, Hae Naem;Kwak, Myeong Ja;Khaine, Inkyin;Lee, Tae Yoon;Lee, Hyun Kyung;Kim, Ie Rae;Jang, Gyeong Hwan;Lee, Wi Young;Kang, Ho Duck;Woo, Su Young
    • Journal of Korean Society of Forest Science
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    • v.106 no.2
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    • pp.186-195
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    • 2017
  • This research aims to study the physiological characteristics of poplar clones in Saemanguem reclaimed land where has lower minerals and bad drainage than those in paddy field. In July and August, different poplar clones including Populus euramericana clone (Venziano and L.Avanzo) and deltoides hybrid clone (97-18 and 97-19) showed different physiological characteristics. Physiological characteristics of P. euramericana clones were better than those of P. deltoides hybrid clones. Especially, the average net photosynthetic rate ($A_{net}$) of clone Venziano were $18.62{\mu}mol\;CO_2\;m^{-2}{\cdot}s^{-1}$ and $17.11{\mu}mol\;CO_2\;m^{-2}{\cdot}s^{-1}$, respectively. Those were the highest numerical values among other poplar clones. On the other, the total chlorophyll and carotenoid contents of P. deltoides hybrid clones were higher than those of P. euramericana clones in July. Particularly, the total chlorophyll content of clone 97-18 was $2.37mg{\cdot}g^{-1}FW$. In August, those of P. deltoides hybrid clones decreased than in July. In term of the physiological activity such as net photosynthetic rate and stomatal conductance of P. deltoides hybrid clones were much lower in August than in July. As a result, physiological characteristics of P. euramericana Venziano clone was superior than the other clones in Short rotation coppice (SRC) in Saemanguem area.

Growth of Minuartia laricina, Arenaria juncea, and Corydalis speciose in Field with Various Soil Water Contents (토양 수분 함량에 따른 너도개미자리, 벼룩이울타리, 산괴불주머니의 노지 생육)

  • Gil, Min;Kwon, Hyuck Hwan;Kwon, Young Hyun;Jung, Mi Jin;Kim, Sang Yong;Rhie, Yong Ha
    • Journal of Bio-Environment Control
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    • v.29 no.4
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    • pp.344-353
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    • 2020
  • Plants native in Korea have not only ornamental values but also have excellent environmental adaptability, so they can be used as garden plants. Studies on proper volumetric water content (VWC) of substrates have been reported, but many have been conducted in glasshouse conditions where environmental factors were controlled. When considering garden planting, it is necessary to perform the automated irrigation system in outdoor conditions where rainfall occurs at frequent intervals. This research aimed to investigate the VWC suitable for the growth of Minuartia laricina, Arenaria juncea, and Corydalis speciosa in open filed. Sandy soil which consisted of particles of weathered rock was used, and the VWC of 0.15, 0.20, 0.25, and 0.30 ㎥·m-3 was maintained using an automated irrigation system with capacitance soil moisture sensors and a data logger. No significant differences in growth and antioxidant enzymes activity of A. juncea were observed among VWC treatments. However, the survival rate was low at VWC 0.30 ㎥·m-3 treatment, which was the highest soil moisture content. Even considering the efficiency of water use, we recommended that VWC 0.15-0.20 ㎥·m-3 is suitable for the cultivation of A. juncea. Minuartia laricina showed better growth with lower VWC. Because of frequent rainfall in open field, plant volume and survival rate was high even in VWC 0.15 ㎥·m-3 treatment. In C. speciosa, the plant height, number of shoots and lateral shoots, and fresh and dry weight were higher in plants grown in VWC 0.25 ㎥·m-3 as compared with that in the plants grown at 0.15, 0.20, and 0.30 ㎥·m-3. Based on these results, M. laricina needed less water in open filed, and A. juncea and C. speciosa required higher VWC, but excessive water should be avoided.

An Analysis of the Impact of Entrepreneurial Activities in Busan on Regional Economic Growth and Reduction of Unemployment Rate (부산지역 창업활동이 지역경제 성장과 실업률 저감에 미치는 영향 분석)

  • Kim, Ji Young;Lee, Ye Lim
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.11 no.6
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    • pp.111-122
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    • 2016
  • Support and efforts are being strengthened at the national level for entrepreneurial activities that are expected to revitalize our economy. Generally, according to the previous studies, it is known that entrepreneurship positively affects economic growth and the reduction of unemployment rate through employment creation. The analysis of the impact of entrepreneurial activities on economic growth and job creation is mainly based on national comparative analysis. In this paper, however, the impact of start-up activities in the Busan metropolitan city on regional economic growth and employment improvement. In this study, the dummy variables were assigned to firms within three years, five years, and ten years after start-up according to the period since the start-up of the firm. As a result of the empirical analysis, the value of dummy variable of the start - up firms was found to be significant in the analysis of the sales growth rate as the dependent variable according to the preceding study by Evans(1987). Therefore, it can be seen that the growth rate of the start - up company is higher than that of the other companies and it is positive for the regional economic growth. In addition, the coefficient of the dummy variable decreases from the enterprise analysis to the latter within three years, five years, and ten years after the start-up, and confirms that the coefficient of the firm's start-up years has a negative value. I could see more clearly. On the other hand, in the analysis of the effect of start-up firms on unemployment rate reduction, the growth rate of regular workers was higher than that of other firms. This suggests that it positively affects the reduction of the unemployment rate in the region. In addition, in the dynamic analysis using the Almon estimation formula and the Koyck model, the effect of log-transformed net start-up firms on the growth rate of new workers is very significant. As a result of the above model, It is reaffirmed that start-up activity has a positive effect on employment growth.

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Conversion of an Anaerobic Digester to Thermophilic Range (혐기성소화조(嫌氣性消和槽)의 고온(高溫)에로의 전환(轉換))

  • Chang, Duk;Chung, Tai Hak
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.6 no.4
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    • pp.21-28
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    • 1986
  • Conversion of anaerobic mesophilic digestion to thermophilic range has been investigated using a synthetic sludge. When temperature was raised at a rate of 2, 1, and $0.5^{\circ}C$ per day with continuous feeding, a lower reaction rate was observed with a high rate of temperature change. Although methane fermentation ceased completely for an digesters at thermophilic temperature, acid fermentation continued. Methane fermentation was never achieved even with neutralization during 6 months of resting. The methane formers were completely inactivated by the temperature shock and accumulation of volatile acids due to continuous feeding, while the acid formers lost biological activity quickly, but gradually acclimated to a high temperature. When temperature was raised without feeding, successful thermophilic digestion was achieved with 1 day of resting at thermophilic temperature at a rate of $1^{\circ}C$ per day, and also achieved with 20 days of resting at a direct increase. Conversion to a thermophilic range is easily achieved with resting. A short period of resting is required at a low rate of temperature increase, while a long period of resting enough to balance methane formers with acid fermers makes a conversion possile when temperature is raised at a high rate. Soured thermophilic digesters were recovered after seeding of mesophilic sludges, and sludge seeding could be a good method of start-up, conversion, or recovery of a thermophilic digester. Significant amount of thermophiles seemed to be present in the mesophilic digesters.

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Relevance of Serum Vitamin D and Indices Related To Cardiovascular Disease Among Korean Adults (한국 성인의 혈청 비타민 D 수준과 심혈관 질환 관련 지표와의 관련성)

  • Kim, Han-Soo;Ryu, So-Yeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.6
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    • pp.365-374
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    • 2018
  • This study investigated the relationship between Vitamin D levels and indices related to cardiovascular disease in Korean adults aged ${\geq}19years$. The data for analysis were obtained from the sixth Korea National Health Nutrition Examination Survey in 2013 and 2014. The results showed that the incidence of Vitamin D deficiency was 73.1% among Korean adults and that indices related to cardiovascular disease showed an increasing trend (55.6% of Korean adults). The relationship between Vitamin D levels and indices related to cardiovascular disease with controlled physical activity status was also studied. The odds ratios (ORs) for age were 1.72 for the 4-62 years age group (95% CI, 1.53-1.93) and 2.05 for the ${\geq}65years$ age group (95% CI, 1.71-2.45). For blood pressure, the OR for pre-hypertension was 1.30 (95% CI, 1.15-1.47) and that for hypertension was 1.31 (95% CI, 1.11-1.54). For body mass index (BMI), the OR was 1.36 (95% CI, 1.11-1.66) and that for waist circumference (WC) was 1.36 (95% CI, 1.11-1.66). For fasting blood sugar (FBS), the OR for impaired fasting glucose (IFG) was 1.37 (95% CI, 1.21-1.55) and that for diabetes mellitus (DM) was 1.31 (95% CI, 1.05-1.65). The OR for total cholesterol (TC) was 1.30 (95% CI, 1.11-1.52) and that for triglycerides (TG) was 1.20 (95% CI, 1.04-1.37) in Korean adults. There was a significant relationship between Vitamin D and indices related to cardiovascular disease in Korean adults with respect to age, blood pressure, FBS, BMI, TC and TG. Confirmation of a causal relationship between Vitamin D and indices related to cardiovascular disease may require further research consisting of more systematic cohort studies.

Study on Anti-inflammatory and Anti-microbial Effect of Pinus rigida Mill. inner Bark Extracts as a Cosmetic Material (리기다소나무(Pinus rigida Mill.) 내수피 추출물에 대한 화장품 소재로써의 항염 및 항균효과)

  • Jang, Min-Jung;Kim, Young-Hun;An, Bong-Jeun;Lee, Chang-Eeon;Lee, Jin-Tae;Kim, Sea-Hyun;Lee, Byung-Guen;Lee, Do-Hyung
    • Journal of Korean Society of Forest Science
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    • v.97 no.3
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    • pp.215-220
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    • 2008
  • Recently, there has been a great deal of interest in the applications of plant-based extracts to both cosmetic and medicinal industries. The objective of this study was to investigate the anti-inflammatory and antimicrobial effect of P. rigida extracts by water and ethyl acetate. Anti-inflammatory and anti-microbial effect of P. rigida extracts by water and EtOAc were investigated by using nitrite scavenging ability, nitric oxide production and anti-microbial ability. In the test of nitrite scavenging ability, P. rigida extracts by water and EtOAc showed 88.7% and 99% at 100 ppm concentration, respectively. The cell viability was measured using the MTT assay at 24 hours after P. rigida extracts as shown in over 80%. Anti-inflammatory effect was examined in LPS stimulated RAW 264.7 cells. NO productions in LPS and P. rigida extracts stimulated group were decreased in a concentration and were dependent on time as compared with LPS stimulated. The water extracts showed the highest inhibition at the 100 ppm concentration. In anti-microbial activity test, the water extract with 3.0 mg/disc resulted in the clear zone of 14 mm, and ethyl acetate with that of 15 mm for Staphylococcus aureus. However, P. rigida extracts didn't show any growth inhibitory effect on Esherichia coli. These results indicate that the extracts of P. rigida have anti-inflammatory activities as a cosmeceuticals.

A Study on Curriculum Development For Community Health Practitioners (보건진료원 직무교육 교과과정 개선을 위한 일 연구)

  • 조원정;이경자
    • Journal of Korean Academy of Nursing
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    • v.22 no.2
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    • pp.207-226
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    • 1992
  • This study was designed to develop a conceptual framework for the curriculum and develop the details of the learning content for the education of Community Health Practitioners (CHPs). Since education programs for CHPs started 10 years ago, concepts related to CHP services have changed because of changes in society. The objectives of the study were as follows : 1) to analyse the usefulness of the present education program for CHPs, 2) to analyse the Job performance and self -confidence of the CHPs, 3) to identify the health needs of the clients served by the CHPs and the community problems related to health. 4) to develop a conceptual framework for the curriculum, for the education of CHPs, 5) to develops details for the learning content of the education program for CHPs. Phase I of the study was conducted by questionnaires to 150 CHPs who have worked in remote rural areas for more than 2 years. Among them, 147 responded. Data was collected from August 16, to August 25, 1990. In order to identify the health needs of the community people, research within the last five years was reviewed and analyzed. The data on 1, 842 communities gathered by the WHO Nursing Collaborations Center of the College of Nursing, Yonsei University was utilized to identify community problems related to health and the self - confidence in job performance of the CHPs. Psase II of the study consisted of a workshop with 13 professionals including Community Health Practitioners to evaluate the existing education program and a conceptual framework of the curriculum for the job education of CHPs. The results of the study are Summariged below : 1. The only 26 among 45 content items of the education program related to job skills was used by 80% of the responding CHPs. The knowledge of $\ulcorner$Networking community organization$\lrcorner$ was used by only 53.7% of the respondents. Educational content about $\ulcorner$Mental disease$\lrcorner$ was used by less than 50% of CHPs because of a knowledge deficit. 2. The CHPs reported that their activities concentrated on clinical services during the last six months. The survey showed that they seemed to neglect the activities for health promotion and disease prevention. Thus, $\ulcorner$Education for community loaders$\lrcorner$(15.9%), $\ulcorner$Activity for eavironmental health$\lrcorner$(16.3%) and $\ulcorner$Social work for needey people$\lrcorner$(23.3%) were done by less than 30% of CHPs. 3. More than 90% of CHPs reported being self - confident for the activities of $\ulcorner$Health education and counselling$\lrcorner$, $\ulcorner$Medicine prescription$\lrcorner$ and $\ulcorner$Immunization$\lrcorner$. But 50% of CHPs reported that they were not have self - confident in $\ulcorner$Management of water and environmental health$\lrcorner$ and only 25.6% of CHPs could insert an IUD independently. 4. It was identified that respiratory diseases and the gastrointestinal diseases were most common problems for the community people, followed by musculoskeletal and skin problems. 5. The community problems were classified into eight categories : physical environmental problems, environmental hygiene, health problems, health behavior, social problem, lack of resources, financial problem and the problems of the cultural and value system. 6. The conceptual framework consisted of the target population and their health status, nursing process working site and primary health care services such as health promotion, disease prevention, treatment and rehabilitation. 7. The contents of curriculum of education program for CHPs were formulated from the results of this study.

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