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A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of 'Candidatus Liberibacter asiaticus' in Citrus

  • Tirumalareddy Danda;Jong-Won Park;Kimberly L. Timmons;Mamoudou Setamou;Eliezer S. Louzada;Madhurababu Kunta
    • The Plant Pathology Journal
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    • v.39 no.4
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    • pp.309-318
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    • 2023
  • Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, 'Candidatus Liberibacter asiaticus' (CLas) is a non-culturable phloem-limited α-proteobacterium transmitted by Asian citrus psyllids (ACP, Diaphorina citri Kuwayama). A widely adopted method for HLB diagnosis is based on quantitative real-time polymerase chain reaction (qPCR). Although HLB diagnostic qPCR provides high sensitivity and good reproducibility, it is limited by time-consuming DNA preparation from plant tissue or ACP and the requirement of proper lab instruments including a thermal cycler to conduct qPCR. In an attempt to develop a quick assay that can be deployed in the field for CLas detection, we developed a real-time loop-mediated isothermal amplification (rt-LAMP) assay by targeting the CLas five copy nrdB gene. The rt-LAMP assay using various plant sample types and psyllids successfully detected the nrdB target as low as ~2.6 Log10 copies. Although the rt-LAMP assay was less sensitive than laboratory-based qPCR (detection limit ~10 copies), the data obtained with citrus leaf and bark and ACP showed that the rt-LAMP assay has >96% CLas detection rate, compared to that of laboratory-based qPCR. However, the CLas detection rate in fibrous roots was significantly decreased compared to qPCR due to low CLas titer in some root DNA sample. We also demonstrated that the rt-LAMP assay can be used with a crude leaf DNA extract which is fully deployable in the field for quick and reliable HLB screening.

Groundwater evaluation in the Bokha watershed of the Namhan River using SWAT-MODFLOW (SWAT-MODFLOW를 활용한 남한강 복하천유역의 지하수 모의 평가)

  • Han, Daeyoung;Lee, Jiwan;Jang, Wonjin;Kim, Seongjoon
    • Journal of Korea Water Resources Association
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    • v.53 no.11
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    • pp.985-997
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    • 2020
  • SWAT (Soil and Water Assessment Tool)-MODFLOW (Modular Groundwater Flow) is a coupled model that linking semi-distributed watershed hydrology with fully-distributed groundwater behavior. In this study, the groundwater simulation results of SWAT and SWAT-MODFLOW were compared for Bokhacheon watershed in Namhan river basin. The models were calibrated and validated with 9 years (2009~2017) daily streamflow (Q) data of Heungcheon (HC) water level gauge station and the daily groundwater level observation data of Yulheon (YH). For SWAT, the groundwater parameters of GW_DELAY, GWQMN, and ALPHA_BF affecting baseflow and recession phase were treated. The SWAT results showed the coefficient of determination (R2) of 0.7 and Nash-Sutcliffe model efficiencies (NESQ, NSEinQ) for Q and 1/Q with 0.73 and -0.1 respectively. For SWAT-MODFLOW, the spatio-temporal aquifer hydraulic conductivity (K, m/day), specific storage (Ss, 1/m), and specific yield (Sy) were applied. The SWAT-MODFLOW showed R2, NSEQ, and NSEinQ of 0.69, 0.74, and 0.51 respectively. The SWAT-MODFLOW considerably enhanced the low flow simulation with the help of aquifer physical information. The total streamflow of SWAT and SWAT-MODFLOW were 718.6 mm and 854.9 mm occupying baseflow of 342.9 mm and 423.5 mm respectively.

ON SOME PROPERTIES OF MALCEV-NEUMANN MODULES

  • Zhao, Renyu;Liu, Zhongkui
    • Bulletin of the Korean Mathematical Society
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    • v.45 no.3
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    • pp.445-456
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    • 2008
  • Let M be a right R-module, G an ordered group and ${\sigma}$ a map from G into the group of automorphisms of R. The conditions under which the Malcev-Neumann module M* ((G)) is a PS module and a p.q.Baer module are investigated in this paper. It is shown that: (1) If $M_R$ is a reduced ${\sigma}$-compatible module, then the Malcev-Neumann module M* ((G)) over a PS-module is also a PS-module; (2) If $M_R$ is a faithful ${\sigma}$-compatible module, then the Malcev-Neumann module M* ((G)) is a p.q.Baer module if and only if the right annihilator of any G-indexed family of cyclic submodules of M in R is generated by an idempotent of R.

SEMI-SYMMETRIC STRUCTURE JACOBI OPERATOR FOR REAL HYPERSURFACES IN THE COMPLEX QUADRIC

  • Imsoon Jeong;Gyu Jong Kim;Changhwa Woo
    • Bulletin of the Korean Mathematical Society
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    • v.60 no.4
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    • pp.849-861
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    • 2023
  • In this paper, we introduce the notion of semi-symmetric structure Jacobi operator for Hopf real hypersufaces in the complex quadric Qm = SOm+2/SOmSO2. Next we prove that there does not exist any Hopf real hypersurface in the complex quadric Qm = SOm+2/SOmSO2 with semi-symmetric structure Jacobi operator. As a corollary, we also get a non-existence property of Hopf real hypersurfaces in the complex quadric Qm with either symmetric (parallel), or recurrent structure Jacobi operator.

REAL HYPERSURFACES IN THE COMPLEX HYPERBOLIC QUADRIC WITH CYCLIC PARALLEL STRUCTURE JACOBI OPERATOR

  • Jin Hong Kim;Hyunjin Lee;Young Jin Suh
    • Journal of the Korean Mathematical Society
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    • v.61 no.2
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    • pp.309-339
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    • 2024
  • Let M be a real hypersurface in the complex hyperbolic quadric Qm*, m ≥ 3. The Riemannian curvature tensor field R of M allows us to define a symmetric Jacobi operator with respect to the Reeb vector field ξ, which is called the structure Jacobi operator Rξ = R( · , ξ)ξ ∈ End(TM). On the other hand, in [20], Semmelmann showed that the cyclic parallelism is equivalent to the Killing property regarding any symmetric tensor. Motivated by his result above, in this paper we consider the cyclic parallelism of the structure Jacobi operator Rξ for a real hypersurface M in the complex hyperbolic quadric Qm*. Furthermore, we give a complete classification of Hopf real hypersurfaces in Qm* with such a property.

NOTES ON THE SPACE OF DIRICHLET TYPE AND WEIGHTED BESOV SPACE

  • Choi, Ki Seong
    • Journal of the Chungcheong Mathematical Society
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    • v.26 no.2
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    • pp.393-402
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    • 2013
  • For 0 < $p$ < ${\infty}$, ${\alpha}$ > -1 and 0 < $r$ < 1, we show that if $f$ is in the space of Dirichlet type $\mathfrak{D}^p_{p-1}$, then ${\int}_{1}^{0}M_{p}^{p}(r,f^{\prime})(1-r)^{p-1}rdr$ < ${\infty}$ and ${\int}_{1}^{0}M_{(2+{\alpha})p}^{(2+{\alpha})p}(r,f^{\prime})(1-r)^{(2+{\alpha})p+{\alpha}}rdr$ < ${\infty}$ where $M_p(r,f)=\[\frac{1}{2{\pi}}{\int}_{0}^{2{\pi}}{\mid}f(re^{it}){\mid}^pdt\]^{1/p}$. For 1 < $p$ < $q$ < ${\infty}$ and ${\alpha}+1$ < $p$, we show that if there exists some positive constant $c$ such that ${\parallel}f{\parallel}_{L^{q(d{\mu})}}{\leq}c{\parallel}f{\parallel}_{\mathfrak{D}^p_{\alpha}}$ for all $f{\in}\mathfrak{D}^p_{\alpha}$, then ${\parallel}f{\parallel}_{L^{q(d{\mu})}}{\leq}c{\parallel}f{\parallel}_{\mathcal{B}_p(q)}$ where $\mathcal{B}_p(q)$ is the weighted Besov space. We also find the condition of measure ${\mu}$ such that ${\sup}_{a{\in}D}{\int}_D(k_a(z)(1-{\mid}a{\mid}^2)^{(p-a-1)})^{q/p}d{\mu}(z)$ < ${\infty}$.

Estimation of Potential Natural Vegetation using the Estimate to Probability Distribution of Vegetation in Bukhansan National Park (식생 분포 확률 추정을 통한 북한산 국립공원의 잠재자연식생 추정)

  • Shin, Jin-Ho;Yeon, Myung-Hun;Yang, Keum-Chul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.16 no.3
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    • pp.41-53
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    • 2013
  • The study for the estimation potential natural vegetation was estimated the occurrence probability distribution using geographic information system(GIS) in Bukhansan National Park. Correlation and factor analysis were analyzed to estimate probability distribution. Coefficients were calculated by logistic regression analysis. Correlation coefficients were significantly at the 0.01 level. Commonality of elevation, annual mean temperature, warmth index and potential evapotranspiration were high value, but topographic index was low value. Communities of over the 0.3 points distribution probability, Quercus mogolica communities were the largest area, 76,940,900 $m^2$, Pinus densiflora communities area was 860,800 $m^2$, Quercus acutissima communities area was 500,100 $m^2$ and Quercus variabilis communities area was 1,000 $m^2$, but Q. aliena, Q. serrata, Carpinus laxiflora and Zelcova serrata communities was not appeared. Therefore, potential national vegetation of Bukhansan national park was likely to be Q. mongolica community, P. densiflora community, Q. acutissima community and Q. variabilis community.

PSEUDO-UNIVERSAL SPACES OF VECTOR BUNDLES

  • SOHN, KYU-HYUN
    • Honam Mathematical Journal
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    • v.3 no.1
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    • pp.41-60
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    • 1981
  • 다양체(多樣體)의 연구(硏究)에서 벡터속(束)의 개념(慨念)은 불가결(不可缺)하며 벡터속(束)의 연구(硏究)에는 엽층구조(葉層構造)의 연구(硏究)가 중요(重要)하다. 본(本) 논문(論文)은 권(圈) $\varrho$(M)의 응사보편공간(凝似普遍空間)에 관한 연구(硏究)(정리(定理) 4.8)로서 R. Bott의 엽층구조(葉層構造)에 관한 연구(硏究)([1])에서 착상(着想)된 것이다. 제이(第二), 삼절(三節)은 제사절(第四節)을 위한 준비(準備)로서, 제이절(第二節)에서는 벡터속(束)및 접속(接續)에 관한 성질(性質)을 논하고, 제삼절(第三節)에서는 위상권(位相圈), 층(層), 엽층구조(葉層構造) 및 $\Gamma_{q}$-cocycle 등에 관한 성질(性質)(명제(命題) 3.5, 3.7과 3.11)을 밝히고, 위상권(位相圈)의 구체적(具體的)인 예(例)(예(例)3.2, 3.3과 3.12)를 들었다. 제사절(第四節)에서는 $GL_{q}-cocycle$, 위상권(位相圈) $GL_{q}$, 집합(集合) $I_{so}(M_{k},\;GL_{q})$, $H^{1}(M_{k},\;GL_{q})$, $I_{so}(\varrho(M))$ 및 응사보편공간(凝似普遍空間)을 정의(定義)하고, 주정리(主定理) 4.8의 증명(證明)에 필요(必要)한 명제(命題)를 몇 개 기술(記述)하였다(명제(命題) 4.2, 4.5와 보제(補題) 4.9).

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