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Specific Primer for Detection of Jujube Witches' Broom Phytoplasma Group (16SrV) in Korea

  • Han, Sang-Sub
    • The Plant Pathology Journal
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    • v.21 no.1
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    • pp.55-58
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    • 2005
  • In order to diagnose and differentiate jujube witches' broom (JWB) phytoplasma rapidly, oligonucleotide primer pair, 16Sr(V) F/R, for polymerase chain reactions (PCRs) was designed on the basis of 16S rRNA sequences of JWB phytoplasma. The PCR employing phytoplasma universal primer pair P1/P7 consistently amplified DNA in all tested phytoplasma isolates. But no phytoplasma DNA was detected from healthy jujube seedlings. The nested PCR, the primer pair 16S(V) F/R, about 460 bp fragment, amplified DNA in all tested JWB and related phytoplasmas including ligustrum witches' broom phytoplasma of the 16S rRNA group V, but no DNA amplification was detected from other phytoplasma strains such as groups 16SrI (Aster yellows) and 16SrXII (Stolbur group) in which mulberry dwarf phytoplasma and chrysanthemum witches' broom phytoplasma belong to, respectively. The same results were obtained from both Korean and Chinese isolates of JWB phytoplasma. Nested-PCR using phytoplasma universal primer pair P1/P7 and 16SrV group-specific primer pair 16S(V) F/R could detect group V phytoplasmas rapidly and easily, in particular JWB phytoplasma.

ESTIMATES FOR RIESZ TRANSFORMS ASSOCIATED WITH SCHRÖDINGER TYPE OPERATORS

  • Wang, Yueshan
    • Bulletin of the Korean Mathematical Society
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    • v.56 no.5
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    • pp.1117-1127
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    • 2019
  • Let ${\mathcal{L}}_2=(-{\Delta})^2+V^2$ be the $Schr{\ddot{o}}dinger$ type operator, where nonnegative potential V belongs to the reverse $H{\ddot{o}}lder$ class $RH_s$, s > n/2. In this paper, we consider the operator $T_{{\alpha},{\beta}}=V^{2{\alpha}}{\mathcal{L}}^{-{\beta}}_2$ and its conjugate $T^*_{{\alpha},{\beta}}$, where $0<{\alpha}{\leq}{\beta}{\leq}1$. We establish the $(L^p,\;L^q)$-boundedness of operator $T_{{\alpha},{\beta}}$ and $T^*_{{\alpha},{\beta}}$, respectively, we also show that $T_{{\alpha},{\beta}}$ is bounded from Hardy type space $H^1_{L_2}({\mathbb{R}}^n)$ into $L^{p_2}({\mathbb{R}}^n)$ and $T^*_{{\alpha},{\beta}}$ is bounded from $L^{p_1}({\mathbb{R}}^n)$ into BMO type space $BMO_{{\mathcal{L}}1}({\mathbb{R}}^n)$, where $p_1={\frac{n}{4({\beta}-{\alpha})}}$, $p_2={\frac{n}{n-4({\beta}-{\alpha})}}$.

A study on the relationship of family support and personality with quality of life in the Patients receiving radiotherapy for cervix cancer (방사선 치료를 받는 자궁경부암 환자의 가족지지와 성격이 삶의 질에 미치는 영향)

  • Noh, Young-Hee
    • The Journal of Korean Society for Radiation Therapy
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    • v.8 no.1
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    • pp.149-159
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    • 1996
  • This descriptive correlational study was undertaken in order to investigate the relationship of family support and personality with quality of life in patients receiving radiotherapy for cervix cancer and to provide basic data to help them improve a better quality of life. The subjects for this study Were 80 out-patients undergoing a radiation therapy at C university hospital in K-city, from April, 1992 to October, 1994. The data were obtained using a convenience sampling technique. The tools used for this study were Ro's quality of life scale, Kang's family support scale and Wallston & others health locus of control scale. The collected data were analyzed by the SAS program using percentage, mean, standard deviation, pearson's correlation coefficients. The result were as follows : 1. The total average score of the quality of life of the subjects was 138.95(minimum score 121-maximum score 164), item mean score(range 1-5) was 2.95. The total average score of the family support of the subjects was 32.55 (minimum score 16-maximum score 47), item mean score(range 1-5) was 2.95 The total average score of the health locus of control of the subjects was 37.00 (minimum score 24-maximum score 49), item mean score (range 1-6) was 3.36. 2 The results of the analysis of the relationship between the quality of life scale and the health locus of control were as follows : the total average score in the quality of life of internal locus of control scale was 136.97, the total average score in the quality of life of external locus of control scale was 144.90. 3. There was a significant positive correlation between the health locus of control and the quality of life(r = 0.2927, p<0.01). The result of the analysis of the relationship between the each factor in the quality of life and health locus of control were as follows : There were significant differences between the health locus of control and emotional state factor(r=0.1514, p<0.01), economic life factor(r=0.2560, p<0.05), self-esteem factor(r=0.2289, p<0.05), physical state and function factor(r=0.1455, p<0.05), relationship with neighbors factor(r=0.0754, p<0.05), relationship with family factor (r=0.3324, p<0.01). 4. There was a significant positive correlation between the family support and the quality of life(r=0.459, p<0.001). The result of the analysis of the relationship between the each factor in the quality of life and family support were as follows : there were significant differences between family support and emotional state factor (r=0.3891, p<0.01), self-esteem factor(r=0.2661, p<0.05), relationship with family factor (r=0.4353, p<0.001).

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Nuclear rDNA characteristics for DNA taxonomy of the centric diatom Chaetoceros (Bacillariophyceae)

  • Oh, Hye-Young;Cheon, Ju-Yong;Lee, Jin-Hwan;Hur, Sung-Bum;Ki, Jang-Seu
    • ALGAE
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    • v.25 no.2
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    • pp.65-70
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    • 2010
  • The genus Chaetoceros provides highly diversified diatoms in marine systems. Morphological descriptions of the genus are well-documented, yet the DNA taxonomy of Chaetoceros has not been satisfactorily established. Here, the molecular divergences of the 18S-28S rDNA of Chaetoceros were assessed. DNA similarities were relatively low in both 18S (93.1 $\pm$ 3.9%) and 28S rDNA (81.0 $\pm$ 4.6%). Phylogenies of the 18S, 28S rDNAs showed that Chaetoceros was divided according to individual species, clustering the same species into single clades. Statistical analysis with corrected genetic (p-) distance scores showed that nucleotide divergence of Chaetoceros 28S rDNA significantly differed from that of 18S rDNA (Student's t-test, p < 0.05). This finding suggests that the 28S rDNA may be treated as a more suitable marker for species-level taxonomic distinctions of Chaetoceros.

A Correlational Study among Perceived Stress, Anger Expression, and Depression in Cancer Patients* (암 환자의 지각된 스트레스, 분노표현방식과 우울간의 관계)

  • Lee Pyong-Sook;Sohn Jung-Nam;Lee, Yong-Mi;Park Eun-Young;Park Ji-Sun
    • Journal of Korean Academy of Nursing
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    • v.35 no.1
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    • pp.195-205
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    • 2005
  • Purpose: This study was to identify the relationship between perceived stress, anger expression, and level of depression in cancer patients. Method: A cross-sectional descriptive study design was used. Data was collected by uestionnaires from 185 in- and out-patients who were diagnosed with cancer at 3 university hospitals and the National Cancer Center using Spielberger et al.’s Anger Expression Scale, Cohen, Kamarch & Mermelstein's Perceived Stress, and Derogatise's SCL-90. The data was analyzed using descriptive statistics, Pearson correlation coefficient, and stepwise multiple regression with SAS/PC. Result: The perceived stress in cancer patients indicated a significant positive correlation to anger-in(r=.288, p=.000), anger-out(r=.232, p=.001), and depression(r=.68, p=.000), but no significant correlation to anger-control. The anger-in of cancer patients showed a significant positive relationship to anger-out(r=.53, p=.000), and depression(r=.383, p=.000), but no significant correlation to anger-control. Anger-out showed a significantly negative correlation to anger-control (r=-.248, p=.001) and a positive correlation to depression (r=.240, p=.001). The most significant predictor which influenced depression in cancer patients was perceived stress, followed by anger-in and hobby, and these factors explained their depression with a variance of 54%. Conclusion: These results suggested that cancer patients with a high degree of perceived stress are likely to be high in anger-out and anger-in. Perceived stress and anger-in are major factors which affect depression in cancer patients.

A Study on the Perceived Stress Level of Mothers in the Neonatal Intensive Care Unit Patients (신생아 중환자실에 입원한 환아 어머니의 스트레스)

  • Kim Tae Im
    • Child Health Nursing Research
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    • v.6 no.2
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    • pp.224-239
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    • 2000
  • This descriptive study was conducted to understand the contents and degree of parental stress level in the NICU patients, and to give a baseline data in developing nursing intervention program. Subjects were the 62 mother of hospitalized newborn in NICU of 1 University Hospital in Taejon City from May 1st, 1999 to November 30th, 1999, who agreed to take part in this study. The instrument used in this study were Parental Stressor Scales : NICU(PSS:NICU) developed by Miles et al. and validated by 3 NICU practitioners and 3 child health nursing faculties. The questionnaire has 4 dimensions and 45 items; sight and sounds of NICU(5 items), babies' appearance and behavior(19 items), parental role alteration and relationship with their baby(10 items), communication with health team(11 items). The questionnaire asks parents to rate each item on a five-point Likert type scale that ranges from (1) to (5). Total scores representing overall stress from the NICU environment are calculated by summing response to each item. A high score indicates high stress. A subscale score is calculated by summing the responses to each item in the subscale. Cronbach's α coefficients were .93. The data was analyzed as average, Frequency, Standard deviation, t-test, ANOVA, Pearson correlation coefficient by use of SPSS/PC+. The results of this study is summarized as follows ; 1. The total perceived stress level score of mothers was slightly high(3.6±.7). The highest scored dimension was 'appearance and behavior of the baby'(3.9±1.5), and next were 'relationship with their baby and parental role change'(3.5±1.4), 'communication with health team'(3.4±.9), 'sight and sounds of NICU'(3.2±.8). 2. Two variables were statistically significant with PSS:NICU total scale ; mother's perceived severity of the baby's condition (r=.482, P=.002) and mother's religious attendance(t=2.83, P=.01). The more the mother perceive their baby's condition severe, the higher the total stress score. There were high stress score noted in the mother of no religious attendance. 3. Four variables were statistically significant with NICU environment subscale ; mother's educational background(F=3.45, P=.04), religious attendance(t=2.28, P=.04), sex of the baby(t=2.83, P=.01) and NICU patients' hospital day(r=.359, P=.004). That is mother with high educational background and girl baby were high NICU environment subscale score. 4. Four variables were statistically significant with appearance and behavior of the baby subscale ; when first saw baby(F=3.52, P=.04), incubator care(t=2.83, P=.01), mother's perceived severity of the baby's condition(r=.303, P=.017), number of NICU visit(r=.441, P=.002). That is, seeing the baby first in the NICU and recieved incubator care was very stressful. Also, the more the mother perceive their baby's condition severe and more NICU visit, the higher the appearance and behavior of the baby subscale stress score. 5. Four variables were statistically significant with relationship with their baby and parental role change subscale ; when first saw baby(F=3.37, P=.04), sex of the baby(t=2.36, P=.03), incubator care(t=5.60, P=.00), mother's perceived severity of the baby's condition(r=.401, P=.001). That is, seeing the baby first in the NICU and girl baby was very stressful. Also, the more the mother perceive their baby's condition severe, the higher the relationship with their baby and parental role change subscale stress score. 6. Three variables were statistically significant with communication with health team subscale ; mother's educational background (F=3.63, P=.04), incubator care(t=4.24, P=.00), gestational age(r=-.394, P=.047), and birth weight(r=-.460, P=.004). That is, mother with high educational background and receiving incubator care were high communication with health team subscale score. Also, the shorter the gestational age and smaller the baby's birth weight, the higher the communication with health team subscale score. In conclusion, information about physical environment of NICU, the mother's perceived severity of baby's illness state, maternal role change related variables and the knowledge of characteristics of NICU patients must be included in nursing intervention program of mother's of NICU patients in reducing the maternal stress and anxiety level.

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Phytoplasma specific primer for detection of jujube witches′ broom group(16SrV) in Korea and China

  • Sangsub Han;Lee, Sanghun;Mengjun Liu;Byeongjin Cha
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.136.2-137
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    • 2003
  • In order to diagnose and differentiate jujube witches' broom (JWB) phytoplasma rapidly, oligonucleotide primer pair, 16Sr(V) F/R, for polymerase chain reactions (PCRs) was designed on the basis of 165 rRNA sequences of JWB phytoplasma. The PCR employing phytoplasma universal primer pair P1/P7 consistently amplified DNA in all tested phytoplasma isolates. But no phytoplasma DNA was detected in healthy jujube seedlings. The nested PCR, the primer pair 16S(V) F/R, about 460 bp fragment, amplified DNA in all tested JWB and related phytoplasmas including LiWB phytoplasma of the 165 rRNA group V, but no DNA amplification was detected from other phytoplasma strains such as group 16SrI (Aster yellows) and group 16SrⅩII (Stolbur group) phytoplasmas in which mulberry dwarf phytoplasma and chrysanthemum witches broom phytoplasma are belonged to, respectively The same results were obtained from both Korean- and Chinese-isolates of JWB. Nested-PCR using phytoplasma universal primer pair P1/P7 and 16S rRNA group V specific primer pair 16S(V) F/R could detect group V phytoplasma rapidly and easily, in particular JWB phytoplasma.

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Chemical accident response competencies and educational needs of 119 EMTs (119 구급대원의 화학사고 대응역량 및 교육요구도)

  • Myeong-Hui Park;Seung-Eun Han
    • The Korean Journal of Emergency Medical Services
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    • v.28 no.1
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    • pp.7-19
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    • 2024
  • Purpose: This study aimed to evaluate and assess the response capabilities and educational needs of 119 emergency medical technicians (EMTs) in chemical accidents. Methods: A self-reported questionnaire was completed by 167 119 EMTs between December 1st and December 31, 2023. The questionnaire comprised 8 questions on general characteristics, 2 on chemical accidents experienced by the participants, 29 on response capabilities, and 15 on educational needs. Data analysis was performed using t-tests, analysis of variance, Duncan's test for post-hoc analysis, and Pearson's correlation coefficient, using SPSS 27.0. Results: The participants scored 2.69 points on response capacity to chemical accidents. The EMT-Paramedics scored high in 'patient triage,' 'patient treatment,' 'patient transport,' and 'collaborative support' (F=3.924, p=.010; F=5.843, p=.001; F=3.698, p=.013; F=5.272, p=.002), followed by educational experience (t=-4.962, p<.001; t=-2.685, p=.008; t=-3.455, p=.001; t=-3.593, p<.001; t=-3.034, p=.003). The participants scored 4.19 points on educational needs, with high scores for 'patients treatment and transport' (4.280.93). The scores for 'patient triage competency', and 'patient triage' (r=.169, p=.024) correlated positively. Furthermore, the scores for 'patient treatment competency' and all sub-factors of educational needs (r=.185, p=.013; r=.215, p=.004; r=.199, p=.008; r=.190, p=.011; r=.197, p=.008) correlated positively. Conclusion: To strengthen the response capabilities of 119 EMTs, it is imperative to develop an educational program that focuses on first-aid responses.

SOME Lq INEQUALITIES FOR POLYNOMIAL

  • Chanam, Barchand;Reingachan, N.;Devi, Khangembam Babina;Devi, Maisnam Triveni;Krishnadas, Kshetrimayum
    • Nonlinear Functional Analysis and Applications
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    • v.26 no.2
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    • pp.331-345
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    • 2021
  • Let p(z)be a polynomial of degree n. Then Bernstein's inequality [12,18] is $${\max\limits_{{\mid}z{\mid}=1}}\;{\mid}p^{\prime}(z){\mid}\;{\leq}\;n\;{\max_{{\mid}z{\mid}=1}{\mid}(z){\mid}}$$. For q > 0, we denote $${\parallel}p{\parallel}_q=\{{\frac{1}{2{\pi}}}{\normalsize\displaystyle\smashmargin{2}{\int\nolimits_{0}}^{2{\pi}}}\;{\mid}p(e^{i{\theta}}){\mid}^qd{\theta}\}^{\frac{1}{q}}$$, and a well-known fact from analysis [17] gives $${{\lim_{q{\rightarrow}{{\infty}}}}\{{\frac{1}{2{\pi}}}{\normalsize\displaystyle\smashmargin{2}{\int\nolimits_{0}}^{2{\pi}}}\;{\mid}p(e^{i{\theta}}){\mid}^qd{\theta}\}^{\frac{1}{q}}={\max\limits_{{\mid}z{\mid}=1}}\;{\mid}p(z){\mid}$$. Above Bernstein's inequality was extended by Zygmund [19] into Lq norm by proving ║p'║q ≤ n║p║q, q ≥ 1. Let p(z) = a0 + ∑n𝜈=𝜇 a𝜈z𝜈, 1 ≤ 𝜇 ≤ n, be a polynomial of degree n having no zero in |z| < k, k ≥ 1. Then for 0 < r ≤ R ≤ k, Aziz and Zargar [4] proved $${\max\limits_{{\mid}z{\mid}=R}}\;{\mid}p^{\prime}(z){\mid}\;{\leq}\;{\frac{nR^{{\mu}-1}(R^{\mu}+k^{\mu})^{{\frac{n}{\mu}}-1}}{(r^{\mu}+k^{\mu})^{\frac{n}{\mu}}}\;{\max\limits_{{\mid}z{\mid}=r}}\;{\mid}p(z){\mid}}$$. In this paper, we obtain the Lq version of the above inequality for q > 0. Further, we extend a result of Aziz and Shah [3] into Lq analogue for q > 0. Our results not only extend some known polynomial inequalities, but also reduce to some interesting results as particular cases.

Mixed Infection of 16S rDNA I and V Groups of Phytoplasma in a Single Jujube Tree

  • Lee, Sang-Hun;Han, Sang-Sub;Cha, Byeong-Jin
    • The Plant Pathology Journal
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    • v.25 no.1
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    • pp.21-25
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    • 2009
  • Jujube trees infected with phytoplasma exhibit symptoms of typical witches' broom, such as yellowing, abnormally small leaves, short internodes and proliferation of shoots. A 1.2 kb fragment of the 16S rDNA from jujube phytoplasma was generated by R16F2n/R16R2 primer pair from earlier amplified P1/P7 PCR products of cloned jujube witches' broom phytoplasmas. Enzymatic restriction fragment length polymorphism (RFLP) and sequence analysis of 16S rDNA revealed that the jujube tree was infected with 16S rDNA I and V groups of phytoplasmas. Extensive comparative analyses of restriction enzyme profiles from Alu I, Hha I, Msp I, and Rsa I clearly classified the two into different phytoplasma groups. The phylogenie analyses based on 16S rDNA showed that the similarity of the two different clones was 87.5%. This is the first report of a mixed phytoplasmal infection in a single jujube tree.