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The Korean Society of Phycology (한국조류학회(藻類))
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Nuclear rDNA characteristics for DNA taxonomy of the centric diatom Chaetoceros (Bacillariophyceae)

  • Oh, Hye-Young (Department of Green Life Science, Sangmyung University) ;
  • Cheon, Ju-Yong (Department of Green Life Science, Sangmyung University) ;
  • Lee, Jin-Hwan (Department of Green Life Science, Sangmyung University) ;
  • Hur, Sung-Bum (Korea Marine Microalgae Culture Center, Department of Aquaculture, Pukyong National University) ;
  • Ki, Jang-Seu (Department of Green Life Science, Sangmyung University)
  • Received : 2010.03.28
  • Accepted : 2010.04.25
  • Published : 2010.06.15
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Abstract

The genus Chaetoceros provides highly diversified diatoms in marine systems. Morphological descriptions of the genus are well-documented, yet the DNA taxonomy of Chaetoceros has not been satisfactorily established. Here, the molecular divergences of the 18S-28S rDNA of Chaetoceros were assessed. DNA similarities were relatively low in both 18S (93.1 $\pm$ 3.9%) and 28S rDNA (81.0 $\pm$ 4.6%). Phylogenies of the 18S, 28S rDNAs showed that Chaetoceros was divided according to individual species, clustering the same species into single clades. Statistical analysis with corrected genetic (p-) distance scores showed that nucleotide divergence of Chaetoceros 28S rDNA significantly differed from that of 18S rDNA (Student's t-test, p < 0.05). This finding suggests that the 28S rDNA may be treated as a more suitable marker for species-level taxonomic distinctions of Chaetoceros.

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References

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