• The Korean Journal of Zoology
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• v.38 no.3
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• pp.340-347
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• 1995

We have used two kinds of P element constructs, Pc[(ry+)B] and p[(ry+)$\Delta$SX9], for genetic transformation by microinjection of D. melanogaster. Pc[(ry+)B] construct carrying the rosy gene within an autonomous P element was injected into a true M strain caring the ry506. mutation. The source of transposase for microinjection and transformation was provided by a P element helper plasmid designated p-$\Delta$2-3hs$\pi$, which was co-injected with nonautonomous P[(ry+)$\Delta$SX9] construct into same ry506 M strains. A dechorination method was adopted and 35 independent transformed lines were obtained froin 1143 G0 Injected (35/1143). About 20% of the injected embryos eclosed as adults. Among G0 eclosed flies, approximately 40% exhibited eye color that was similar to wild-type (ry+), but about 60% of fertile G0 transformed lines appeared to have no G1 transformants. Therefore it is unlikely that G0 expression requires integration of the rosy transposon into chromosomes. Pc[(ry+)B] and P[(ry+)$\Delta$SX9] constructs were found to be nearly same in the frequency of element-mediated transformation. On the basis of these results, nonautonomous P elements constructs could he used as same effective vectors in P element-mediated transformation for introducing and fixing genes in insect populations.

• 한국전산유체공학회:학술대회논문집
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• 2009.04a
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• pp.245-251
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• 2009

Numerical algorithms for solving the incompressible Navier-Stokes equations using P2P1 finite element are compared regarding the eigenvalues of matrices. P2P1 element allocates pressure at vertex nodes and velocity at both vertex and mid nodes. Therefore, compared to the P1P1 element, the number of pressure variables in the P2P1 element decreases to 1/4 in the case of two-dimensional problems and to 1/8 in the three-dimensional problems. Fully-implicit-integrated, semi-implicit- integrated and semi-segregated finite element formulations using P2P1 element are compared in terms of elapsed time, accuracy and eigenvlue distribution (condition number). For the comparison,they have been applied to the well-known benchmark problems. That is, the two-dimensional unsteady flows around a fixed circular cylinder and decaying vortex flow are adopted to check spatial accuracy.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.33 no.4
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• pp.242-247
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• 2009

AILU type preconditioners for a two-dimensional combined P2P1 finite element formulation of the interaction of rigid cylinder with incompressible fluid flow have been devised and tested by solving fluid-structure interaction (FSI) problems. The FSI code simulating the interaction of a rigid cylinder with an unsteady flow is based on P2P1 mixed finite element formulation coupled with combined formulation. Four different preconditioners were devised for the two-dimensional combined P2P1 finite element formulation extending the idea of Nam et al., which was proposed for the preconditioning of a P2P1 mixed finite element formulation of the incompressible Navier-Stokes equations. It was found that PC-III or PC-IV among them perform well with respect to computational memory and convergence rate for some bench-mark problems.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.345-350
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• 2000

Adaptive finite element analysis, which its solution error meets with the user defined allowable error, is recently used far improving reliability of finite element analysis results. This adaptive analysis is composed of two procedures; one is the error estimation of an analysis result and another is the reconstruction of finite elements. In the rp-method, an element size is controlled by relocating of nodal positions(r-method) and the order of an element shape function is determined by the hierarchical polynomial(p-method) corresponding to the element solution error. In order to show the effectiveness and accuracy of the suggested rp-method, various numerical examples were analyzed and these analysis results were examined by comparing with those obtained by the existed methods. As a result of this study, following conclusions are obtained. (1) rp-method is more accurate and effective than the r- and p-method. (2) The solution convergency of the rp-method is controlled by means of the iterative calculation numbers of the r- and p- method each other.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.4 no.1
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• pp.67-77
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• 2000

In this paper we consider finite element mathods for nonlinear parabolic problems defined in ${\Omega}{\subset}R^d$ ($d{\leq}4$). A new initial approximation is taken. Optimal order error estimates in $L_p$ for $2{\leq}p{\leq}{\infty}$ are established for arbitrary order finite element. One order superconvergence in $W^{1,p}$ for $2{\leq}q{\leq}{\infty}$ are demonstrated as well.

• Structural Engineering and Mechanics
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• v.22 no.2
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• pp.151-167
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• 2006

A p-version of the finite element method in conjunction with the modeling dynamic method using the arc-length stretch deformation is considered to determine the bending natural frequencies of a cantilever flexible plate mounted on the periphery of a rotating hub. The plate Fourier p-element is used to set up the linear equations of motion. The transverse displacements are formulated in terms of cubic polynomials functions used generally in FEM plus a variable number of trigonometric shapes functions representing the internals DOF for the plate element. Trigonometric enriched stiffness, mass and centrifugal stiffness matrices are derived using symbolic computation. The convergence properties of the rotating plate Fourier p-element proposed and the results are in good agreement with the work of other investigators. From the results of the computation, the influences of rotating speed, aspect ratio, Poisson's ratio and the hub radius on the natural frequencies are investigated.

• Journal of Korean Association for Spatial Structures
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• v.8 no.4
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• pp.81-90
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• 2008

This paper presents a finite element formulation for the three-dimensional hierarchical solid element using Integrals of Legendre polynomials. The proposed hexahedral solid element is composed of four different modes including vertex, edge, face, and internal mode, respectively. The eigenvalue and patch test have been carried out to confirm the zero-energy mode and constant strain condition. In addition to these, a posteriori error estimation has been studied for the p-adaptive finite element analysis that is based on a smoothing technique to compute a post-processed solution from the finite element solution. The uniform p-refinement and non-uniform p-refinement are compared in terms of convergence rate as the number of degree of freedom is increased. The simple cantilever beam is tested to show the performance of the proposed solid element.

• BMB Reports
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• v.32 no.1
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• pp.45-50
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• 1999

We previously found three transcription factor-binding motifs in the rat p53 promoter. They are two recognition motifs of NF1-like protein (NF1-like element 1: -296 ~ -312, NF1-like element 2: -195 ~ -219) and a bHLH protein binding element (-142 ~ -146). In this study, we investigated the DNA-protein complex formation of the three elements with nuclear extracts from both normal and regenerating liver to find the element involved in the induced transcription of p53. The level of each DNA-protein complex on NF1-like and bHLH motifs was not changed. Instead, a new element located at -264 ~ -284 was detected in the DNase I footprinting assay with regenerating nuclear extract. This element has partial homology to the AP1 consensus motif. However, the competition studies with diverse oligonucleotides suggest that the binding protein is not AP1. An in vitro transcription assay shows that this element is important for the transcriptional activation of the rat p53 promoter. Therefore, for the induced transcription of the rat p53 promoter, the-264 ~ -284 region is required in addition to two NF1-like and one bHLH motif.

• Proceedings of the KSME Conference
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• 2001.06e
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• pp.386-391
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• 2001

A finite element code based on P2P1 tetra element has been developed for the large eddy simulation (LES) of turbulent flows around a complex geometry. Fractional 4-step algorithm is employed to obtain time accurate solution since it is less expensive than the integrated formulation, in which the velocity and pressure fields are solved at the same time. Crank-Nicolson method is used for second order temporal discretization and Galerkin method is adopted for spatial discretization. For very high Reynolds number flows, which would require a formidable number of nodes to resolve the flow field, SUPG (Streamline Upwind Petrov-Galerkin) method is applied to the quadratic interpolation function for velocity variables, Noting that the calculation of intrinsic time scale is very complicated when using SUPG for quadratic tetra element of velocity variables, the present study uses a unique intrinsic time scale proposed by Codina et al. since it makes the present three-dimensional unstructured code much simpler in terms of implementing SUPG. In order to see the effect of numerical diffusion caused by using an upwind scheme (SUPG), those obtained from P2P1 Galerkin method and P2P1 Petrov-Galerkin approach are compared for the flow around a sphere at some Reynolds number. Smagorinsky model is adopted as subgrid scale models in the context of P2P1 finite element method. As a benchmark problem for code validation, turbulent flows around a sphere and a MIRA model have been studied at various Reynolds numbers.

• Geotechnical Engineering
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• v.13 no.1
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• pp.101-110
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• 1997

This paper presents a two dimensional p-version static infinite element for analyzing $1/r^n$ displacement decay type problems in unbounded media. The proposed element is developed by using shape functions based on approximate expressions of an analytical solution. Numerical results are presented for an opening in a homogeneous elastic infinite medium and a rigid footing rested on a homogeneous elastic half-space. The numerical results show the effectiveness of the proposed infinite element.