• Molecules and Cells
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• 제22권3호
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• pp.300-307
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• 2006

Brassica rapa ssp. pekinensis (Chinese cabbage) is an economically important crop and a model plant for studies on polyploidization and phenotypic evolution. To gain an insight into the structure of the B. rapa genome we analyzed 12,017 BAC-end sequences for the presence of transposable elements (TEs), SSRs, centromeric satellite repeats and genes, and similarity to the closely related genome of Arabidopsis thaliana. TEs were estimated to occupy 14% of the genome, with 12.3% of the genome represented by retrotransposons. It was estimated that the B. rapa genome contains 43,000 genes, 1.6 times greater than the genome of A. thaliana. A number of centromeric satellite sequences, representing variations of a 176-bp consensus sequence, were identified. This sequence has undergone rapid evolution within the B. rapa genome and has diverged among the related species of Brassicaceae. A study of SSRs demonstrated a non-random distribution with a greater abundance within predicted intergenic regions. Our results provide an initial characterization of the genome of B. rapa and provide the basis for detailed analysis through whole-genome sequencing.

• 농업과학연구
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• 제35권2호
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• pp.247-262
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• 2008

The purpose of this study is to evaluate farmer's general contents on the current direct payment schemes and to derive some improvement measures, based on the survey data for the three types of direct payment schemes currently executed in Korea; Rice Farmer's Income Support, Early Retirement Aged Farmer's Support and Less Favored Area Direct Payment. In recent years we have introduced diverse direct payment schemes that are expected to have immediate effects in a short term period without enough preparation of the policy, which raises some contradiction between the agricultural policy and the original purpose of the direct payments. The result shows some important revisions should be made related the direct payment schemes, such as farmer's income stability through the improvement of unit payment and the payment length jointed with criteria of the payment, in addition to keeping up multifunctionality of agriculture and enhancing the effect of structural adjustment in agriculture.

• 한국환경농학회지
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• 제22권3호
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• pp.210-214
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• 2003

대전시 노은 도매시장에서 채취한 6종의 채소류 중 농약잔류량을 조사한 후 이에 대한 위해성 평가를 실시하였다. 전체 100건의 분석시료 중 농약이 검출된 시료의 비율은 46.0%이었고, 잔류허용기준 초과비율은 6.0% 이었다. 검출비율이 높은 시료는 상추(85.0%), 깻잎(80.0%), 오이(60.0%) 순이었고, 상추 3건, 깻잎 3건이 허용기준을 초과하였다. 살균제는 dicarboximide계, 유기염소계, azole계 농약, 살충제는 유기인계, 합성 pyrethroid계 농약이 많이 검출되었다. 검출빈도가 높은 성분은 procymidone, chlorpyrifos, chlorothalonil, cypermethrin, EPN의 순이었다. 위해성을 평가하기 위해서 각 농작물에 대한 농약의 추정 섭취량과 ADI를 비교한 결괴 허용기준을 초과한 깻잎의 bitertanol, triflumizole, iprobenphos가 다른 성분에 비하여 높은 비율을 보였다. 그러나 각 농산물의 식이섭취량이 적어 ADI에 대한 추정섭취량의 비율이 0.46% 이하로 나타나 그 영향이 매우 낮은 것으로 판단되었다.

• 현장농수산연구지
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• 제23권2호
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• pp.87-97
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• 2021

한국농수산대학 재학생 및 졸업생 151명을 대상으로 병해충 관리 현황을 조사하기 위해 설문조사를 수행하였다. 설문 내용은 기본문항과 병해충 관리 문항으로 구성되어 있다. 기본문항에는 응답자의 연령, 학적상태, 재배작물, 재배면적등을 포함하고 있으며, 병해충 관리 문항에는 병해충 방제 방법, 농약 선택 근거, 병해충 예찰 방법 등을 포함하고 있다. 기본문항의 응답을 요약하면 전체 응답자 중에서 20대 응답자가 91.2%로 가장 높았고, 응답자의 재배 면적은 3헥터 이상이 34.5%로 가장 높은 비중을 차지하고 있었다. 병해충 관리 문항의 응답을 요약하면 병해충 관리 방식에서는 화학적 방제법이 66%로 가장 높은 비중을 차지하였다. 농약 선택 근거에서는 본인 스스로 기존의 방제 기술을 토대로 결정(30%)하거나 농약 판매상(29%)의 결정을 따랐다. 병해충 예찰 방법은 농촌진흥청 소속기관(29%)과 국가농작물병해충관리시스템(27%)을 활용하는 것으로 나타났다. 농약 판매상의 병해충 진단과 농약 처방에 대한 질문에 응답자의 97%가 보통 이상의 신뢰도를 보였으나 강한 신뢰도를 선택한 경우는 전혀 없었다. 병해충 진단 및 농약 처방을 위한 전문인력 양성 필요성에 대해서는 응답자의 79%가 높은 필요성이 있는 것으로 응답하였는데, 특히, 응답자의 47%는 매우 강한 필요성이 있다고 응답하였다. 이러한 결과는, 병해충 진단과 농약 처방을 위한 정교한 기술을 지닌 병해충 관리를 위한 전문인력이 농업 현장에 필요함을 의미한다. 농민의 시각에서 조사한 설문 결과는 병해충 관리 현황을 이해하고 향후 발전 방향을 제시하는 데 중요한 정보를 제공해 줄 것이다.

• The Plant Pathology Journal
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• 제35권4호
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• pp.381-388
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• 2019

For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Their fulllength sequences share 98.9-99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.

• 한국환경농학회지
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• 제23권1호
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• pp.47-51
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• 2004

In order to know the residual pattern of pesticides and predict to the degradation period until below MRL we experimented procymidone and chlorothalonil for grape which were the most detected pesticide in grape by NAQS(National Agricultural product Quality management Service) survey. In this experiment we sprayed those pesticides 10 days before harvest and analyzed 0, 1, 2, 3, 5, 7, 10 day sample to establish logical equation and to calculate $DT_{50}$. Also the same day samples stored at $4^{\circ}C$ and $20^{\circ}C$, which were compared their degradation patterns. During the cultivating period, the residue amount of procymidone was changed from 1.85 mg/kg (0 day) to 0.33 mg/kg (10 day), $DT_{50}$ was 3.5 days, and chlorothalonil was changed from 5.5 mg/kg (0 day) to 3.49 mg/kg (10 day), $DT_{50}$ was 4.4 days. During the storage period, $DT_{50}$ of procymidone and chlorothalonil at $4^{\circ}C$ were 10.5 and 7.6 days, and 6.3 and 6.1 days at $20^{\circ}C$, respectively.

• 한국균학회지
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• 제49권4호
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• pp.477-486
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• 2021

A survey of insect-associated fungi in Korea revealed a novel fungal strain isolated from the bean bug Riptortus clavatus (Heteroptera: Alydidae). Culturally and morphologically, the fungal strain designated KNUF-20-INY03, shares features with members of the genus Acaulium. Phylogenetic analyses based on the concatenated nucleotide sequences of the internal transcribed spacer regions (ITS) regions and partial sequences of the translation elongation factor 1-alpha (TEF1-α), and β-tubulin (β-TUB), and large subunit of the nuclear ribosomal RNA (LSU) genes showed that the isolate is part of a clade that includes other Acaulium species, but it occupies a distinct phylogenetic position. Based on the shape, size, and color of its conidia and conidiogenous cells, strain KNUF-20-INY03 is readily distinguishable from the closely related A. acremonium, A. albonigrescens, A. caviariformis, A. pannemaniae, and A. retardatum. The conidial length-to-width ratio (1.6) of the novel isolate is significantly lower than that of A. acremonium (1.9), A. albonigrescens (2.4), and A. pannemaniae (2.4), and KNUF-20-INY03 produces hyaline conidia and elliptical conidiogenous cells while A. caviariformis forms brown conidia and A. retardatum produces flask-shaped conidiogenous cells. Thus, both phylogenetic and morphological analyses indicate that this strain is a novel species in the genus Acaulium, and we propose the name Acaulium microspora sp. nov.

• Journal of Forest and Environmental Science
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• 제26권3호
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• pp.149-159
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• 2010

This paper analyzes data on the composition, status, diversity, and distribution pattern of invasive forest undergrowth in a protected area (Khadimnagar National Park) of Northeastern Bangladesh. Assessment was done by means of stratified random sampling to diversify the invasive forest undergrowth species. For vegetation survey, 45 plots were taken randomly in ($2m{\times}2m$) circular plot from three topographical regions namely top of the hill, middle slope and plain land (15 plots from each region) and a total of 715 individuals, 22 invasive species belonging to 17 families were recorded from the study site. Among invasive species, shrubs constitute 10 species, herbs 9 species, and vines 3 species respectively. Mass number of invasive undergrowth species was grows in plain land (45.45%) followed by middle slope (31.82%). Based on the survey, invasive undergrowth plants of study areas were also categorized into three degrees of invasiveness e.g., highly invasive, moderately invasive and potentially invasive. Herbs, shrubs, and vines constitute the highest density at Chromolaema odorata (Linn.) King. (1.09), relative density at Chromolaema odorata (Linn.) King. 6.85%; highest and lowest frequency was calculated at Cassia alata L. (64.44%) and Diplazium esculentum (24.44%); for relative frequency the highest was Cassia alata L., which occupies 6.64%. Determination of the abundance of the different species revealed that Cassia alata L., constitutes (3.36) followed by Pteris cretica Wilsonii (3.14) of the area. The presence of invasive undergrowth species always reduced the number of associated species. Therefore, an extensive in-depth long-term investigation, proper policy formulation and management interventions and further study and continuous monitoring on their impacts need to be triggered targeting the control of the invasive undergrowth species of this protected area. In this aspect, national and international organization could help to conserve its biodiversity.

• 농업과학연구
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• 제33권1호
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• pp.73-84
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• 2006

The purposes of this study were to investigate the influence of local residents' nature environment attitudes for National Park development impact perceptions and to examine application possibility of nature environment attitude for resolution information of National Park management conflicts. Thirty items of residents' perceptions and opinions were designed from previous tourism impact studies. The new environmental paradigm(NEP) was used to measure local residents' nature environment attitudes. In April, 2003, 239 questionnaires were obtained from interview survey in local residents' villages near Gyearyongsan National Park. Generally, most of the local residents perceived low economic effects and negative environment impacts for National Park development. Nature environment attitudes levels were significant differences with socioeconomic variables of local residents, specially high in age, education level and influenced their perceptions toward National Park development impacts. The results were suggested that the nature environment attitudes could be provided a useful reverential framework in resolution of National Park management conflicts.

• 현장농수산연구지
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• 제21권2호
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• pp.103-114
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• 2019

본 연구에서는 한농대에 재학 중인 3학년 학생을 대상으로 대학생활 선호도 및 졸업 후 영농의지를 파악하기 위하여 설문조사를 실시하였다. 연구 분석에는 구조화되지 않은 데이터의 분석 기법으로 오피니언 마이닝과 텍스트 마이닝 기법을 이용하였으며, 텍스트 마이닝의 결과는 워드 클라우드로 시각화하여 정보를 추출하였다. 또한 감성분석 결과를 이용하여 졸업 후 농사일을 하려는 학생들의 영농의지에 대한 통계적 분석을 하였다. 대학생활 호감도 조사는 대학 이미지, 자기 역량, 기숙사, 교육시스템, 미래 비전 등 5개 분야에 전체 10개 항목에 대하여 이루어졌다. 감성 분석을 위한 긍·부정 사전은 수집된 응답지에서 긍정과 부정의 감정을 분류하여 긍정어 사전과 부정어 사전을 각각 만들어 분석에 이용하였다. 분석 결과 10개 평가항목 가운데 대학 지원 당시의 '대학 이미지', 10년 후의 '자기 모습' 항목은 70% 이상, '자기 역량'과 '현재의 한농대' 항목은 60% 이상의 긍정적 감정을 나타냈다. 반면 '대학 기숙사' '교육과정' '장기현장실습' '한국 농업의 미래' 항목에 대해서는 긍정적 감성보다 부정적 감성이 높게 나타났다. 성별, 영농기반, 입학 동기에 따른 영농의지 차이의 교차 분석에서는 성별, 입학 동기에 따른 영농의지는 통계적으로 유의미한 결과가 나타났으나, 영농기반에서는 유의미하지 않은 결과가 나타났다. 또한 영농의지에 대한 이항 로지스틱 회귀분석에서는 통계적으로 유의미한 변수는 '입학 동기'로 파악되었으며, 본인의 의지로 입학한 학생일수록 영농의지가 형성될 확률이 높게 나타났다.