• Journal of Microbiology and Biotechnology
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• v.21 no.10
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• pp.1057-1063
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• 2011

Herein, a novel ginsenosidase, named ginsenosidase type IV, hydrolyzing 6-O-multi-glycosides of protopanaxatriol-type ginsenosides (PPT), such as Re, R1, Rf, and Rg2, was isolated from the Aspergillus sp. 39g strain, purified, and characterized. Ginsenosidase type IV was able to hydrolyze the 6-O-${\alpha}$-L-($1{\rightarrow}2$)-rhamnoside of Re and the 6-O-${\beta}$-D-($1{\rightarrow}2$)-xyloside of R1 into ginsenoside Rg1. Subsequently, it could hydrolyze the 6-O-${\beta}$-D-glucoside of Rg1 into F1. Similarly, it was able to hydrolyze the 6-O-$_{\alpha}$-L-($1{\rightarrow}2$)-rhamnoside of Rg2 and the 6-O-${\beta}$-D-($1{\rightarrow}2$)-glucoside of Rf into Rh1, and then further hydrolyze Rh1 into its aglycone. However, ginsenosidase type IV could not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadiol-type ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd. These exhibited properties are significantly different from those of glycosidases described in Enzyme Nomenclature by the NC-IUBMB. The optimal temperature and pH for ginsenosidase type IV were $40^{\circ}C$ and 6.0, respectively. The activity of ginsenosidase type IV was slightly improved by the $Mg^{2+}$ ion, and inhibited by $Cu^{2+}$ and $Fe^{2+}$ ions. The molecular mass of the enzyme, based on SDS-PAGE, was noted as being approximately 56 kDa.

• Proceedings of the KIEE Conference
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• 2015.07a
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• pp.665-666
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• 2015

In this paper, it is proposed to substitute the tilting operation device of hybrid multi-D.O.F system from three phase SPM to single phase torque actuator. When using tilting operation with three phase SPM which is appropriate for continuous rotary operation, there are disadvantages that electricity availability rate is low and controllability is difficult. On the other hand when using a single phase torque actuator instead of SPM, it is appropriate for sequential tilting, electricity availability rate is high, and it is easy to control. Thus this will have a great influence on the development and practical use of the system. This study designed a single phase torque actuator in consideration of the magnetic saturation. Also, this was verified with comparative analysis with SPM through FEM(finite element method) analysis values of the design model and test values of the manufactured model.

• The Journal of Information Technology
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• v.5 no.4
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• pp.73-79
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• 2002

In this paper, I will propose the Niche-Meta Genetic Algorithm that has a multi-mutation operator for design of fuzzy controller. The gene in the proposed algorithm is formed by several parameters that represent the crossover rate, mutation rate and input-output membership functions. The optimization of fuzzy membership function is performed with local search on sub-population and the optimal structure is constructed with global search on total-population. The multi-mutation is selected under basis of the result of local evolution. A simulation for 2 D.O.F wheeled-mobile robot is showed to prove the efficiency of the proposed algorithm

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.1
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• pp.57-63
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• 2012

A surfaced permanent magnet spherical motor is capable of operating as three degree of freedom that used for the joints of the robot's arm, leg, and eyes. Ongoing research like new concept is essential part of motor field, it will make a great contribution in the future the overall portion of the motor, is becoming expected. The author analysis torque characteristics in spherical motor with state of rotating and positioning. And future design direction is smaller motors with equivalent or higher output. Solutions as torque and efficiency improvements are selecting the core with special processing type like powder metallurgy materials. Their special characteristic is high permeability and low eddy current losses at high speed, so improved the torque and efficiency.

• Proceedings of the IEEK Conference
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• 2002.07a
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• pp.58-61
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• 2002

In this paper, we present a new generalized k-Tree-based (CU/PE) allocation model to perform dynamic resource (CU/PE) allocation/deallocation decision for the reconfigurable MSIMD/MIMD multi-dimensional (k-D) mesh-connected architectures. Those reconfigurable multi-SIMD/MIMD systems allow dynamic modes of executing tasks, which are SIMD and MIMD. The MIMD task requires only the free sub-system; however the SIMD task needs not only the free sub-system but also the corresponding free CU. In our new k-Tree-based (CU/PE) allocation model, we introduce two best-fit heuristics for the CU allocation decision: 1) the CU depth first search (CU-DFS) in O(kN$_{f}$ ) time and 2) the CU adjacent search (CU-AS) in O(k2$^{k}$ ) time. By the simulation study, the system performance of these two CU allocation strategies was also investigated. Our simulation results showed that the CU-AS and CU-DFS strategies performed the same system performance when applied for the reconfigurable MSIMD/MIMD 2-D and 3-D mesh-connected architectures.

• Journal of Microbiology and Biotechnology
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• v.22 no.3
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• pp.343-351
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• 2012

In this paper, the kinetics of a cloned special glucosidase, named ginsenosidase type III hydrolyzing 3-O-glucoside of multi-protopanaxadiol (PPD)-type ginsenosides, were investigated. The gene (bgpA) encoding this enzyme was cloned from a Terrabacter ginsenosidimutans strain and then expressed in E. coli cells. Ginsenosidase type III was able to hydrolyze 3-O-glucoside of multi-PPD-type ginsenosides. For instance, it was able to hydrolyze the 3-O-${\beta}$-D-(1${\rightarrow}$2)-glucopyranosyl of Rb1 to gypenoside XVII, and then to further hydrolyze the 3-O-${\beta}$-D-glucopyranosyl of gypenoside XVII to gypenoside LXXV. Similarly, the enzyme could hydrolyze the glucopyranosyls linked to the 3-O-position of Rb2, Rc, Rd, Rb3, and Rg3. With a larger enzyme reaction $K_m$ value, there was a slower enzyme reaction speed; and the larger the enzyme reaction $V_{max}$ value, the faster the enzyme reaction speed was. The $K_m$ values from small to large were 3.85 mM for Rc, 4.08 mM for Rb1, 8.85 mM for Rb3, 9.09 mM for Rb2, 9.70 mM for Rg3(S), 11.4 mM for Rd and 12.9 mM for F2; and $V_{max}$ value from large to small was 23.2 mM/h for Rc, 16.6 mM/h for Rb1, 14.6 mM/h for Rb3, 14.3 mM/h for Rb2, 1.81mM/h for Rg3(S), 1.40 mM/h for Rd, and 0.41 mM/h for F2. According to the $V_{max}$ and $K_m$ values of the ginsenosidase type III, the hydrolysis speed of these substrates by the enzyme was Rc>Rb1>Rb3>Rb2>Rg3(S)>Rd>F2 in order.

• Transactions of the Korean Society of Automotive Engineers
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• v.12 no.6
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• pp.175-181
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• 2004

This paper presents the main method to analyze the dynamic characteristics of power transmission system using the multi-body dynamics, which is based on the concept of subsystem equation, subsystem assembling, and the self-determination technique for the system degree of freedom. We can model the mechanical components of power transmission system easily with the advantage of multi-body dynamics. Based on the theory, a dynamic simulation program was developed to analyze system performances, transient phenomena, and other dynamic problems. The driving performance of automatic transmission was simulated with using the multi-body dynamics and Newtonian method, and the validity of program was proved by comparing the two kinds of result.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.8
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• pp.1227-1234
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• 2001

A multi d.o.f robotic manipulator is considered for multi-axis vibration control of a slender structure, using the concept of the flow source based vibration control. In order not to cause the motion saturation of the manipulator system, a hybrid dynamics associated with the flexible and desired manipulator error dynamics is also modeled as the control object. It is numerically shown that the flexible vibrations and the base motions of a test structure can be effectively controlled with the proposed hybrid dynamics.

• Proceedings of the KSME Conference
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• 2001.06a
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• pp.837-841
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• 2001

The column-type sensing element in building and mechanical construction parts was designed as three forces and three moments sensor by attaching strain gages approximately. Compared to conventional multi-component sensor, the designed sensor can solve the problem about low stiffness and high cost. The radius of the column was designed analytically and compared with finite element analysis. The coupling errors between components were minimized by using addition and subtraction procedure of signals. The fabricated sensor was tested by using a deadweight force standard machine and a six-component force calibration machine in Korea Research Institute of Standards and Science(KRISS). The calibration showed that the multi-component force/moment sensor had coupling error less than 19.8 % between $F_x$ and $M_y$ components, and 9.0 % in case of other components.

• Journal of computational fluids engineering
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• v.10 no.2
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• pp.38-47
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• 2005

Substructuring methods are often used in finite element structural analyses. In this study a multi-level substructuring(MLSS) algorithm is developed and proposed as a possible candidate for finite element fluid solvers. The present algorithm consists of four stages such as a gathering, a condensing, a solving and a scattering stage. At each level, a predetermined number of elements are gathered and condensed to form an element of higher level. At the highest level, each sub-domain consists of only one super-element. Thus, the inversion process of a stiffness matrix associated with internal degrees of freedom of each sub-domain has been replaced by a sequential static condensation of gathered element matrices. The global algebraic system arising from the assembly of each sub-domain matrices is solved using a well-known iterative solver such as the conjugare gradient(CG) or the conjugate gradient squared(CGS) method. A time comparison with CG has been performed on a 2-D Poisson problem. With one domain the computing time by MLSS is comparable with that by CG up to about 260,000 d.o.f. For 263,169 d.o.f using 8 x 8 sub-domains, the time by MLSS is reduced to a value less than $30\%$ of that by CG. The lid-driven cavity problem has been solved for Re = 3200 using the element interpolation degree(Deg.) up to cubic. in this case, preconditioning techniques usually accompanied by iterative solvers are not needed. Finite element formulation for the incompressible flow has been stabilized by a modified residual procedure proposed by Ilinca et al.[9].