• 대한수의학회지
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• 제38권2호
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• pp.359-365
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• 1998

This study was conducted to isolate the protozoan parasite Babesia gibsoni by intraerythrocytic culture method of micoraerophilous stationary phase(MASP) and evaluate the possibility of application for the detection of B gibsoni in canine babesiosis. Also, indirect fluorescent antibody test(IFAT) and thick blood smear(giemsa stain), direct light microscopy (DLM), as control diagnostic tests, were conducted to compare diagnostic effects between MASP, IFAT and DLM. The results obtained from this study were summarized as follows. The protozoan parasite B gibsoni multiplied in 24-well polystyrene plate containing 1.2ml of canine red blood cell suspension in RPMI 1640 medium(pH 7.0) which is contained 20~40% normal canine serum(NCS) under the MASP condition of 5% $CO_2$ and 95% air at $37^{\circ}C$ incubator. Under the above MASP culturing system the percentage of parasitized erythrocytes(PPE) after incubation for 9 days reached the peak. The levels of PPE in MASP culture were shown more higher by exchanging the medium at 24 hour intervals. The parasite were purely isolated from MASP culture of canine red blood cells collected from dogs(pit bullterrier) infected with B gibsoni naturally. Among the total of 83 heads of pit bullterrier blood samples the positive rate was 32 heads(38.5%) in DLM, 45 heads(54.2%) in IFAT and 42 heads(50.6) in MASP culture. In negative cases of IFAT and DLM the isolation rates of B gibsoni by MASP culture were 16 heads(42.1%) of 38 heads and 16 heads(28.6%)% of 56 heads, respectively. From this study it was suggested that MASP culture method by RPMI 1640 medium was a reliable and useful diagnostic test for the diagnosis of B gibsoni infections in canine babesiosis.

• 대한치과기공학회지
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• 제31권3호
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• pp.21-26
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• 2009

Purpose: Standards of alloy for porcelain fused to metal crown be classified by metallic factor and biological factor. Metallic factors consist of stability of alloy composition and mechanical strength and surface characteristics for chemical bond. Biological factors be considered properties of metallic elements and problems originated by toxicity and hypersensitive reaction. Alloys considered such controversial points are the most suitable alloy for dental instrument. Method: Alloys added Be and Nb using Ni-Cr alloy which has been widely used for dental instrument be selected and classified experimental group. Non-addition Be and Nb to Ni-Cr alloy classify control group and addition Be alloy is Be-experimental group, addition Nb alloy is Nb-experimental group. Specimens for cytotoxicity analysis gave effect to washing and sterilization. and then made an experiment on elution with cell medium after disinfection. It conducted specimens within cell medium with 24hours, 48hours, 72hours, respectively. It cultured human dermal fibroblast(HDF) using cell medium for cytotoxicity test and then investigated elution rate through spectroscopic analysis by MTT-assay. Result: As results of cytotoxicity test by MTT-assay, cultured cell rate of VII measured more low numerical value within elution medium for 24hours focused on control group. Also, cultured cell rate of K3 alloys observed low value for 48hours, 72hours than value of control group. Conclusion: According to final result that synthesize above results, Ni-Cr alloy added Be and Ni has little difference in Cytotoxicity by MTT-assay.

• 한국의류학회지
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• 제34권7호
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• pp.1149-1161
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• 2010

This study identifies the dimensions of cosmetics consumption values and the differences in cosmetics brand selection through a cosmetics consumption value group and by product type. The subjects of the study were females over age 20 living in Seoul and Gyeonggi Province between January and February 2008; 427 questionnaires were used for analysis. For data analysis the SPSS 17.0 statistical program was used, and principal component analysis, factor analysis using Varimax rotation, Cronbach's ${\alpha}$ reliability testing, cluster analysis, ANOVA test, and Duncan test were conducted. The results and conclusions of this study are as follows. The dimensions of cosmetics consumption values were found to be the pleasure value, the fashion value, the function value, the brand ostentation value, and the appearance ostentation value. Five types of groups by factor were identified: the group seeking function, the group seeking fashion and brand ostentation, the group seeking pleasure, the group seeking appearance and brand ostentation, and the indifferent group. Second, The group attaching importance to functionality was more likely to select high-priced brands while the group attaching importance to brand awareness were more likely to seek foreign brands, irrespective of product type. As far as base and color cosmetics are concerned, the group attaching importance to pleasure was more likely to select low, medium, and high priced foreign brands, as well as low and medium priced domestic brands. As for body products, the group attaching importance to fashion and brand awareness tended to select low, medium, and high priced domestic brands, as well as high priced foreign brands. By simultaneously purchasing high, medium, and low priced brands, these groups display an ambivalent consumption pattern. This study identified the differing dimensions around cosmetics consumption values and cosmetic brand selection. The research findings helps cosmetic companies set product prices and contributes to cosmetic marketing strategies.

• 디지털융복합연구
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• 제18권10호
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• pp.265-275
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• 2020

본 연구의 목적은 중소병원 수술실 간호사의 간호근무환경과 직무몰입의 관계에서 심리적 임파워먼트의 매개효과를 규명하기 위함이다. 연구 대상은 전국 24개 중소병원의 수술실에서 근무하는 간호사 150명으로, 구조화된 설문지를 이용하여 2018년 7월부터 9월까지 자료를 수집하였다. 수집된 자료는 t-test, ANOVA, Pearson's correlation coefficients, 회귀분석으로 분석하였다. 연구결과, 직무몰입은 간호근무환경 또는 심리적 임파워먼트와 정 상관관계가 있었고. 심리적 임파워먼트는 간호근무환경과 정 상관관계가 있었다. 심리적 임파워먼트는 간호근무환경이 직무몰입에 미치는 영향을 부분 매개하는 것으로 나타났다 중소병원 수술실 간호사들이 간호근무환경을 긍정적으로 인식하여 직무에 몰입하도록 돕기 위해 심리적 임파워먼트 향상 프로그램을 개발하여 적용하는 것이 필요하다.

• 융합정보논문지
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• 제10권3호
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• pp.22-30
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• 2020

본 연구의 목적은 중소병원 간호사의 사회적 지지와 직무만족이 직무배태성에 미치는 영향을 확인하는 것이다. G시에 소재한 중소병원 간호사를 대상으로 하였으며, 197명의 자료를 분석하였다. 자료는 기술적 통계, t-test, ANOVA, Pearson 상관분석, 위계적 회귀분석을 실시하였다. 연구결과 직무배태성은 사회적 지지 중 상사 지지, 동료지지, 직무만족과 정적 상관관계를 보였다. 연구모델은 직무배태성의 28.0%를 설명하였고, 이 중 직무배태성에 영향을 미치는 가장 강력한 변인은 직무만족(β=.33 p<.001)이었으며, 사회적 지지 중 상사지지가 직무배태성에 영향을 미치는 것으로 나타났다. 따라서 중소병원 간호사의 직무배태성을 촉진하기 위해 직무만족 및 사회적 지지의 상사지지를 증진시키기 위한 방안 마련이 필요함을 시사한다.

• 한국정보시스템학회지:정보시스템연구
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• 제28권2호
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• pp.53-72
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• 2019

Purpose The purpose of this study is to examine the impact of tie strength in the network of industrial clustering on the knowledge acquisition, integration and innovation performance of small and medium sized enterprises. We test the positive relationship of weak tie and knowledge acquisition, strong tie and knowledge integration, and the interaction effect of two tie strengths on both processes of knowledge acquisition and integration. By identifying these relationships, we can better understand how to manage the attributes of social networks in terms of tie strength in order to improve the performance of innovation for the small and medium sized enterprises. Design/methodology/approach We collect 200 survey data from 2 industrial cluster respectively: Pankyo and Guroo. In Pankyo, the proportion of IT industry is the highest (35%) while the proportion of manufacturing is highest (35%) in Guroo. Pooling the data from two industrial cluster, we check the reliability and validity of our research model and test the hypotheses. Findings First, we find the positive relationship of weak tie and knowledge acquisition from both industrial clustering. Weak tie is composed of heterogeneous organizations with various background and expertise. The communication and information sharing of organizations in the weak tie network helps the idea generation for organization's innovation, which is the knowledge acquisition process. Second, the relationship of strong tie and knowledge integration is insignificant. Typically the strong tie from long-lasting partnership is expected to be beneficial in the action stage of innovation, which is the knowledge integration process. However it is not identified in our industry cluster. Finally, the interaction effect of weak and strong tie is identified to be effective on both knowledge acquisition and integration processes.

• 융합정보논문지
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• 제9권9호
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• pp.147-157
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• 2019

본 연구는 교사효능감이 보육교사의 행복감에 미치는 영향과 교사-유아 상호작용의 매개효과 및 직무스트레스의 조절효과를 검증하는 것을 목적으로 한다. 이를 위하여 한국아동패널 7차년도 데이터를 활용하여 만 5세 유아 348명과 해당 유아의 담임교사인 보육교사 348명을 분석 대상으로 하였다. 연구방법은 위계적 회귀분석을 통해 연구모형을 검증했다. 매개효과는 Sobel test를 활용하여 검증하였다. 분석결과 첫째 보육교사의 교수효능감이 행복감에 정적인 영향을 미쳤으며, 둘째 교수효능감이 행복감에 미치는 영향에 있어서 교사-유아의 상호작용이 부분매개효과가 있는 것으로 나타났다. 셋째 교수효능감이 보육교사의 행복감에 미치는 영향에 있어 직무스트레스의 조절효과가 나타났다. 이를 토대로 하여 보육교사의 행복감을 높이기 위해 실천적 방안을 모색하고자 한다.

• 융합정보논문지
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• 제11권4호
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• pp.211-219
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• 2021

본 연구 목적은 중소병원 간호사의 언어폭력 경험, 감정노동과 건강증진행위 정도를 알아보고 이들 간의 관련성을 확인하고자 함이다. 대상자는 138명의 간호사로 2개 중소병원에서 모집하였고, 자료분석은 SPSS 20.0 프로그램을 이용하여 기술통계, t-test, ANOVA, Person's Correlation으로 분석하였다. 연구결과, 언어폭력은 82.7%가 경험하였고, 성별(p=.032), 부서(p=.009)에 따라 건강증진행위 정도에 차이가 있었다. 또한 감정노동은 건강증진행위(p=.039)와 관련이 있고 감정노동은 건강증진행위의 하부요인인 영적 성장(p=<.01)과 스트레스 관리(p=.028)와 관련이 있음을 확인하였다. 본 연구는 감정노동을 감소시키고 건강증진 활동을 높이는 전략 및 맞춤형 프로그램 개발에 기초자료로 활용될 것이며 간호 인력의 안정적 확보에도 기여할 것으로 기대한다.

• 한국가축번식학회지
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• 제19권4호
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• pp.259-264
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• 1996

This study was carried out to investigate on the survival rates and in vitro developmental rates of bisected bovine embryos were by manipulator and micropipette. Bisected embryos were co-cultured in 20% FCS(v/v)＋TCM-199 media containing hormones, oviductal epithelial cells and cumulus cells 0 to 72 hrs after bisection. Survival rate and in vitro fertilization rate were defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro developmental rate of biseciton embryos co-cultured in 20% FCS＋TCM-199 medium containing PMSG, hCG, PMSG＋hCG, hCG＋$\beta$-estradiol 0 to 20 hrs and 20 to 40 hrs were 36.7, 26.7, 33.3, 40.0, and 30.0, and 30.0, 33.3, 30.0, 26.7, and 26.7%, respectively. The survival rate of bisection embryos co-cultured in TCM-199 medium containing hormones was significantly higher than that of non co-culture(25.0%). 2. The in vitro developmental rates of bisection embryos co-cultured in 20% FCS＋TCM-199 medium containing oviductal epithelial cells 4 to 5 hrs and 20 to 24 hrs were 40.0 and 33.3%, respectively. The survival rate of bisection embryos co-cultured in TCM-199 medium containing oviductal epithelial cells was significantly higher than that of non co-culture(25.0%). 3. The in vitro developmental rates of bisection embryos co-cultured in 20% FCS＋TCM-199 medium containing cumulus cells 4 to 5 hrs and 20 to 24 hrs were 43.3 and 36.7%, respectively. The survival rate of bisection embryos co-cultured in TCM-199 medium containing cumulus cells was significantly higher than that of non co-culture (25.0%).

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.43-43
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• 2001

This study evaluated the effect of fertilization-promoting peptide (FPP) on fertilizing ability and glycosidase activity in vitro of spermatozoa frozen-thawed in pig, Using chlortetracycline fluorescence analysis, the various glycosidase analyses and the oocyte penetration test, we have obtained evidence that FPP can promote the fertilizing ability and glycosidase activity of frozen-thawed spermatozoa in vitro. When frozen-thawed spermatozoa was washed with different concentrations of FPP, there were significantly (P<0.05) more acrosome-reacted in medium with 100 nM than 0, 50, 200 and 400 nM. The penetration rates were also highest in medium containing with 100 nM FPP (P<0.05). On the other hand, the $\beta$-N-acetylglucosaminidase activity was at least twofold higher than other glycosidase. In same glycosidase, however, there were no difference in medium with different concentrations of FPP In another experiment, spermatozoa preincubated in medium with or without FPP for 0, 1, 2, 3 and 4 h were inseminated with oocytes matured in vitro. The percentages of spermatozoa that reached acrosome reaction were affected by preincubation and were higher in medium with that than without FPP. When oocytes were inseminated with spermatozoa preincubated in medium with and without FPP during the different periods, however, penetration rates were decreased with preincubation periods of spermatozoa. On the other hand, when the sperm-oocyte were cultured for 4, 8, 12, 16, 20 and 24 h, the penetration rates were higher in spermatozoa preincubated with that than without FPP and had a tendency to increase as time of culture periods. However, The activities of $\alpha$-fucosidase, $\alpha$ -mannosidase, $\beta$-galactosidase and N-acetyl- $\beta$-D-glucosaminidase were higher in medium with that than without FPP regardless of periods of sperm preincubation and sperm-oocyte culture. These results suggest that FPP may play a positive role in promoting of sperm function and glycosidase activity in vitro in pig.