• Journal of Animal Reproduction and Biotechnology
• /
• v.35 no.1
• /
• pp.86-93
• /
• 2020

The establishment of porcine embryonic stem cells (ESCs) from porcine somatic cell nuclear transfer (SCNT) blastocysts is influenced by in vitro culture day of porcine reconstructed embryo and feeder cell type. Therefore, the objective of the present study was to determine the optimal in vitro culture period for reconstructed porcine SCNT embryos and mouse embryonic fibroblast (MEF) feeder cell type for enhancing colony formation efficiency from the inner cell mass (ICM) of porcine SCNT blastocysts and their outgrowth. As the results, porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days showed significantly increased efficiency in the formation of colonies, compared to those for 7 days. Moreover, MEF feeder cells derived from outbred ICR mice showed numerically the highest efficiency of colony formation in blastocysts produced through in vitro culture of porcine SCNT embryos for 8 days and porcine ESCs with typical ESC morphology were maintained more successfully over Passage 2 on outbred ICR mice-derived MEF feeder cells than on MEF feeder cells derived from inbred C57BL/6 and hybrid B6CBAF1 mice. Overall, the harmonization of porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days and MEF feeder cells derived from outbred ICR mice will greatly contribute to the successful establishment of ESCs derived from porcine SCNT blastocysts.

• Molecules and Cells
• /
• v.40 no.2
• /
• pp.117-122
• /
• 2017

Despite the fact that porcine embryonic stem cells (ESCs) are a practical study tool, in vitro long-term maintenance of these cells is difficult in a two-dimensional (2D) microenvironment using cellular niche or extracellular matrix proteins. However, a three-dimensional (3D) microenvironment, similar to that enclosing the inner cell mass of the blastocyst, may improve in vitro maintenance of self-renewal. Accordingly, as a first step toward constructing a 3D microenvironment optimized to maintain porcine ESC self-renewal, we investigated different culture dimensions for porcine ICM-derived cells to enhance the maintenance of self-renewal. Porcine ICM-derived cells were cultured in agarose-based 3D hydrogel with self-renewal-friendly mechanics and in 2D culture plates with or without feeder cells. Subsequently, the effects of the 3D microenvironment on maintenance of self-renewal were identified by analyzing colony formation and morphology, alkaline phosphatase (AP) activity, and transcriptional and translational regulation of self-renewal-related genes. The 3D microenvironment using a 1.5% (w/v) agarose-based 3D hydrogel resulted in significantly more colonies with stereoscopic morphology, significantly improved AP activity, and increased protein expression of self-renewal-related genes compared to those in the 2D microenvironment. These results demonstrate that self-renewal of porcine ICM-derived cells can be maintained more effectively in a 3D microenvironment than in a 2D microenvironment. These results will help develop novel culture systems for ICM-derived cells derived from diverse species, which will contribute to stimulating basic and applicable studies related to ESCs.

• Food Science of Animal Resources
• /
• v.44 no.3
• /
• pp.533-550
• /
• 2024

Peptides with bioactive effects are being researched for various purposes. However, there is a lack of overall research on pork-derived peptides. In this study, we reviewed the process of obtaining bioactive peptides, available analytical methods, and the study of bioactive peptides derived from pork. Pepsin and trypsin, two representative protein digestive enzymes in the body, are hydrolyzed by other cofactors to produce peptides. Bicinchoninic acid assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, chromatography, and in vitro digestion simulation systems are utilized to analyze bioactive peptides for protein digestibility and molecular weight distribution. Pork-derived peptides mainly exhibit antioxidant and antihypertensive activities. The antioxidant activity of bioactive peptides increases the accessibility of amino acid residues by disrupting the three-dimensional structure of proteins, affecting free radical scavenging, reactive oxygen species inactivation, and metal ion chelating. In addition, the antihypertensive activity decreases angiotensin II production by inhibiting angiotensin converting enzyme and suppresses blood pressure by blocking the AT1 receptor. Pork-derived bioactive peptides, primarily obtained using papain and pepsin, exhibit significant antioxidant and antihypertensive activities, with most having low molecular weights below 1 kDa. This study may aid in the future development of bioactive peptides and serve as a valuable reference for pork-derived peptides.

• Proceedings of the Zoological Society Korea Conference
• /
• 2005.08a
• /
• pp.26-26
• /
• 2005

• Journal of applied mathematics & informatics
• /
• v.5 no.3
• /
• pp.691-700
• /
• 1998

Simulation algorithms for one-sided and two-sided trun-cated gamma distributions are proposed. These algorithms suggest the optimal choice of derived functions. Some results of simulation are given. Finally an application with real data is presented.

• Communications for Statistical Applications and Methods
• /
• v.2 no.1
• /
• pp.1-12
• /
• 1995

An estimator of coefficients of polynomial measurement error model with vector predictor and first-order interaction terms is derived using Hermite polynomial. Asymptotic normality of estimator is provided and some simulation study is performed to compare the small sample properties of derived estimator with those of OLS estimator.

• Proceedings of the Korean Society for Language and Information Conference
• /
• 2002.02a
• /
• pp.195-204
• /
• 2002

The purpose of this paper is to analyze the lexical-semantic structure of morphologically derived passive verbs in Korean based on Pustejovsky (1995)'s Generative Lexicon Theory (GL) and to explain the change of the root verb's lexical-semantic structure by means of passivization. Passivization in this paper is defined as the unaccusaztivization. In Argument Structure of derived passive verbs, the agent argument is deleted and the theme argument is realized as a syntactic subject. As for Event Structure, derived passives express left-headed event (achievement), whereas their roots denote right-headed event (accomplishment). In Qualia Structure, passive verbs and root ones have the same Fomal Role, but in Agentive Role of passive verbs, an act weakens to a process. Both Formal and Agentive Roles have the same theme argument.

• Korean Journal of Veterinary Research
• /
• v.36 no.2
• /
• pp.453-461
• /
• 1996

Eighteen holstein-calves(4~5 months old) in a divided groups including the matched control were immunized with $100{\mu}g/dose$ of 34kDa, 45kDa polypeptide and T sergenti merozoite vaccine(protein content $100{\mu}g/dose$) respectively, previously mixed with aluminium hydroxide to elicit antibodies. All groups of calves were boosted with same dose and intervals. The animals were challenged by tick infestations in the endemic pasture of theileriosis from March to September 1994. The animals were monitored for the erythrocyte count, parasitemia, hematocrit and the specific antibody reactions elicited by immunization. The immunological responses demonstrated that vaccination with 34kDa polypeptide and T sergenti merozoite derived vaccine inhibited to produce the 75kDa band immunological responds even in the vaccinated calves after being challenged by tick infestations in the pasture. However, the specific antibody reactions were detected at the 32kDa band in the nonimmunized calves and T sergenti merozoite derived vaccine by the western blot. The 34kDa polypeptide vaccine and T sergenti merozoite derived vaccine were evaluated to be able to protect inducing anemia and to decrease parasitemias level. These vaccines have the efficacy of inhibition to produce a certain antigen corresponding 75kDa band antigen of parasite in the calves as challenged with tick infestations.

• Korean Institute of Interior Design Journal
• /
• no.31
• /
• pp.56-63
• /
• 2002

This study intends to prepare a system that can be used, by applying digital technique, in analyzing complexity of architectural forms that have been visualized by the correlation based on the distribution chart made in accordance with profile lines. The profile lines are derived from the edge analysis of the architectural forms, simplified based on the visual theory. For the purpose, this study was conducted in the following ways: First, problems of the existing models for the elevation analysis were examined along with formal analysis based on visual recognition to consider the profile lines derived from the forms. Secondly, in elevation analysis, profile lines were derived by digital method to measure them qualitatively. To verify the objectivity of the measured data value, a survey was conducted based on the adjective cataloging method, and the correlation of the survey result and analyzed data was analyzed to verify the validity of the derived data. Thirdly, supplementation for the problems deducted from experiments and the possibility to use it in designing were suggested. Digital method has many advantages over the conventional analyzing system in deriving precise data value by excluding subjectivity. It also allows various analytical methods in analyzing numerous data repeatedly. Diversified models and methods of analysis considering numerous factors arising in the process of designing remain assignments to research in future.

• Journal of the Korean Society of Costume
• /
• v.49
• /
• pp.113-124
• /
• 1999

The purpose of this study was to categorize brand-switching behaviors into subdivided groups by variety seeking and perceived differences between brands and to compare and to analyze brand-switching motive perceived differences between brands clothing-involvement and buying price among the subdivided groups. The subjects were 343 female and male to have jean pants-buying experience in their age of 20-29 living in Seoul and Kyunggi province. For the analysis of the data mean frequency t-test $\chi^2$-Test Factor analysis MANOVA Duncan-test were conducted and the cronbach's $\alpha$ has been calculated to measure the reliability The results are as follows: 1. brand-switching behaviors are categorized into 4 groups by strength of variety-seeking and perceived differences between brands that are small or large which are habitual brand switchers internal derived brand-switchers external derived brand-switchers and combined derived brand-switchers. 2. Among the 4 sub-groups there were significant difference in brand-switching motive according to factors such as change-seeking needs and price & promotion. And there were significant differences in perceived differences between brands according to factors such as apparel design clothing -symbolism fitness except apparel shop. 3. According to strength of variety-seeking levels of clothing-involvement and buying price have increased Combined derived brand-switchers have totally the strongest variety-seeking and the highest clothin-involvement and they purchase the highest priced jean-pants of the other types of consumers.