• Title/Summary/Keyword: Individuals' Resistance

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Biological Control of Thrips Using a Self-produced Predatory Mite Stratiolaelaps scimitus (Acari: Laelapidae) in the Greenhouse Chrysanthemum (시설재배지 국화에서 자가생산한 뿌리이리응애 (응애아강: 가시진드기과)를 활용한 총채벌레의 생물적 방제)

  • Jung, Duck-Oung;Hwang, Hwal-Su;Kim, San-Young;Lee, Kyeong-Yeoll
    • Korean journal of applied entomology
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    • v.58 no.3
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    • pp.233-238
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    • 2019
  • For greenhouse crops, thrips is one of the major insect pests, but its control is difficult owing to short generation time, rapid escaping behavior, and development of pesticide resistance. Stratiolaelaps scimitus (Womersley) is a soil-dwelling predatory mite attacking various soil invertebrate species, including thrips. Using the method by growers' self-production, we mass-reared S. scimitus colony and investigated thrips control in the greenhouse chrysanthemum. The initial density of thrips was six individuals/flower. The treatment with one S. scimitus box in the greenhouse was estimated to be $1,000individuals/m^2$. From August to September 2018, the greenhouse was released with a total of 10 boxes of S. scimitus. The density of thrips in the untreated and the treated cultivar was $53.7{\pm}7.0$ and $13.5{\pm}1.7$ on the late September, respectively, which indicated a reduction of 74.9% in the treated cultivar. Our results suggest that S. scimitus was highly effective for the control of thrips in the greenhouse chrysanthemum although temperature is very high during the summer season.

Effects of autumn olive berry extract on insulin resistance and non-alcoholic fatty liver in high fructose-fed rat (고과당식이를 급여한 흰쥐에 있어서 토종보리수 추출물의 인슐린 저항성 및 비알콜성 지방간 개선 효과)

  • Ha-Neul Choi;Jihye Choi;Jung-In Kim
    • Journal of Nutrition and Health
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    • v.56 no.6
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    • pp.629-640
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    • 2023
  • Purpose: Non-alcoholic fatty liver disease (NAFLD) is characterized by the accumulation of fat in the liver which is not a result of excessive alcohol consumption. Its global prevalence was estimated to be approximately 32% in the years 1994-2019. More than half of obese individuals and patients with diabetes are reported to have NAFLD as a comorbidity. This study aimed to investigate the impact of the autumn olive (Elaeagnus umbellata Thunb.) berry on insulin resistance and steatosis in rats fed a high-fructose diet. Methods: Six-week-old Wistar rats were divided into four groups. The control group received a diet consisting of 65% corn starch, while the fructose and experimental groups were fed a diet comprising 65% fructose (FRU) and an FRU diet containing 0.5% (low-dose autumn olive berry group; LAO) or 1.0% (high-dose autumn olive berry group; HAO) ethanol extract of autumn olive berry, respectively, for 10 weeks. Results: The HAO group exhibited significantly lower blood glucose levels compared to the fructose-fed group. Both the LAO and HAO groups showed a substantial reduction in serum insulin levels and insulin resistance when compared to the fructose-fed group. The consumption of LAO and HAO significantly ameliorated dyslipidemia and reduced the levels of triglycerides in the liver compared to the fructose-fed group. Additionally, the consumption of HAO resulted in lower serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities compared to the fructose group. The hepatic expression of the sterol regulatory element-binding protein-1c (SREBP-1c) and carbohydrate-responsive element-binding protein (ChREBP) was significantly reduced in the LAO and HAO groups compared to the fructose group. Conclusion: Autumn olive berries improved steatosis by ameliorating insulin resistance and down-regulating the lipogenesis proteins in rats fed on high fructose diet.

Genetic Variations Analysis and Characterization of the Fifth Intron of Porcine NRAMP1 Gene

  • Yan, X.M.;Ren, J.;Ai, H.S.;Ding, N.S.;Gao, J.;Guo, Y.M.;Chen, C.Y.;Ma, J.W.;Shu, Q.L.;Huang, L.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.9
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    • pp.1183-1187
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    • 2004
  • The natural resistance-associated macrophage protein 1 (NRAMP1) gene was identified as a candidate gene controlling the resistance and susceptibility to a number of intracellular parasites in pigs. The genetic variations in a 1.6 kb region spanning exon 1 and exon 3 of the porcine NRAMP1 gene were investigated by PCR-HinfI-RFLP in samples of 1347 individuals from 21 Chinese indigenous pig populations and 3 western pig breeds. Three alleles (A, B, C) and four genotypes (AA, BB, AB, BC) were detected. Significant differences in genotype and allele frequencies were observed between Chinese indigenous pig populations and exotic pig breeds, while in general the differences in genotype and allele frequencies among Chinese indigenous pig populations were not significant. The allele C was detected only in Duroc, Leping Spotted and Dongxiang Spotted pig, and the two Chinese pig populations showed similar genotype and allele frequencies. Four Chinese Tibetan pig populations displayed genetic differentiation at the NRAMP1 gene locus. In addition, intron 5 of the NRAMP1 gene was isolated and characterized by directly sequencing the PCR products encompassing intron 5. The alignment of intron 5 of the porcine, human, equine and ovine NRAMP1 gene showed a similarity of 45.38% between pig and human, 52.55% between pig and horse, 63.47% between pig and sheep, respectively.

Investigation about the Context in which the Effects of Entertainment-education Program May Reduce -Focused on Persuasion Knowledge and Pre-attitude (교육적 오락물의 설득효과 감소요인에 대한 탐색적 연구 -설득지식과 사전태도의 영향을 중심으로)

  • Kim, In-Sung;Lee, Seung-Jo
    • Korean journal of communication and information
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    • v.69
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    • pp.7-29
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    • 2015
  • The purpose of this research is to examine the impacts of persuasion knowledge and pre-attitude on responses to a persuasive entertainment education program. Persuasion knowledge model was adopted as the theoretical frame and information about the sensational identity of entertainment program was used to activate audience's persuasion knowledge(i.e. resistance). Pre-attitude was measured by the degree of concern about the persuasion topic. The experiment was designed by persuasion knowledge(2) ${\times}$ pre-attitude(2) and participants were randomly assigned in one of the conditions and rated their attitude for the persuasion topic. The implicit measure for attitude was included in addition to the explicit measures in order to reveal unconscious attitudinal changes. The results showed that the allegedly effective entertainment-education can confront the resistance of the audience in some contexts. A significant interaction of knowledge ${\times}$ pre-attitude was found in the result of the implicit attitude. The individuals with high concern about the persuasion topic, compared to those with low concern, were more influenced by the entertainment program and also persuasion knowledge. The interaction was not significant on the explicit attitude, which may present the usefulness of the implicit measure in entertainment-education research.

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Community Structure and Cluster Analysis of the Benthic Macroinvertebrates in Inflow and Outflow area of Ten Reservoirs of the Nakdong River System (낙동강수계 10개 호소의 유입 및 유출부의 저서성대형무척추동물 군집구조 및 유사도 분석)

  • Lee, Mi-Jin;Park, Jin-Young;Seo, Jung-Kwan;Lee, Hae-Jin;Seo, Eul-Won;Lee, Jong-Eun
    • Journal of Life Science
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    • v.19 no.12
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    • pp.1758-1763
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    • 2009
  • Biological assessments of benthic macroinvertebrates community structure were carried out in ten reservoir inflow and outflow areas of the Nakdong river system from April 2008 to August 2008. The collected benthic macroinvertebrates from the surveyed sites were 9,427.6 inds./$m^2$, 42 species belonging to 29 families and 16 orders. A dominant species was Chironomus sp. and a subdominant species was Ecdyonurus levis. In the all sites, Ephemeroptera had the most number of species (17.81%) and Diptera had the most number of individuals (64.00%). Dominance, species diversity, species richness, and species evenness indexes were 0.68, 2.42, 2.96 and 0.54, respectively. The numbers of benthic macroinvertebrates species in each surveyed site were from 21 (site 9 Jilnal wetland) to 51 (site 2 Yeongcheon reservoir). As a result of an analysis about relative resistance and resilience of stability factors, the most popular group was group I with high indices in both resistance and resilience. Site 7 (Beongae wetland) and site 8 (Jangcheok reservoir) had the highest similarity in the result of cluster analysis using the surveyed benthic macroinvertebrates.

Validation of Segmental Multi-Frequency Bioelectrical Impedance Analysis based on the Segmental Bioelectrical Impedance analysis in the Elderly Population (분절임피던스를 기준한 분절다주파수 생체임피던스의 일치도 분석)

  • Tang, Sae-Jo;Kim, Jang-Hee;Eom, Jin Jong;Eom, Sunho;Kim, Hakkyun;Kim, Chul-Hyun
    • Journal of Platform Technology
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    • v.9 no.2
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    • pp.38-45
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    • 2021
  • A frequently used bioimpedance analytical method in Korea is the segmental multi-frequency BIA (SMF-BIA) method, but it is not directly determined at a segmented impedance. This study was to compare SMF-BIA determinations with direct segmented determinations for accuracy and appropriateness of segment parameters. This study is to compare the segment parameters, accuracy and appropriateness of the multi-frequency segmental bioimpedance analysis. To this end, 108 elderly individuals were measured. Segmented bioelectrical measurements obtained from a SMF-BIA (Inbody S10) at 50 kHz and measured with a phase sensitive single frequency device (SF-BIA, bia-101, RJL / akern systems) were compared. The significant difference (%) was demonstrated between single - and multiple frequency determinations of the right upper limb (R = 35.5 ± 6.2%, P < 0.001; Xc = 2.7 ± 7.6%, P < 0.01), left upper limb difference (R= 33. 9 ± 6.0%, P < 0.001; Xc = 2.8 ± 8.3%, P < 0.01), right lower limb difference (R = 18.6 ± 4.3%, P < 0.001; Xc = 25.8 ± 10.0%, P < 0.001), left lower limb difference (R = 18.0 ± 4.7%, P < 0.001; Xc = 31.8%). Of the results determined with the two BIA methods, the impedance measurements of the limbs and whole body showed a high correlation (RA: R = 0. 950, LA: R = 0. 949, RL: R = 0.899, LL: R = 0.88), and in the agreement test, the impedance values of the upper limbs and whole body also showed strong agreement (ICC > 0.9), but in the Xc, the correlation was weak. In conclusion, it was found that although bioimpedance devices had significantly different characteristics and inconsistent cross sectionally, there was a high population level agreement in the upper and lower extremities in determining segmental resistance value changes. But a large error was found on the trunk. Further studies were needed for reducing the error.

Characteristics of Antidiabetic Effect of Dioscorea rhizoma(1) - Hypoglycemic Effect - (산약의 항당뇨 특성 연구(1) - 혈당 강하 효과 -)

  • Kang, Tong-Ho;Choi, Sang-Zin;Lee, Tae-Ho;Son, Mi-Won;Kim, Sun-Yeou
    • The Korean Journal of Food And Nutrition
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    • v.21 no.4
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    • pp.425-429
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    • 2008
  • Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. Type 1 diabetes, or juvenile-onset diabetes, results from a cellular-mediated autoimmune destruction of the ${\beta}$-cells of the pancreas. Type 2 diabetes, or adult-onset diabetes, is a term used for individuals who have insulin resistance, a condition that makes it harder for the cells to properly use insulin, and usually have relative insulin deficiency. The diabetes causes the onset of chronic complications and diabetic neuropathy is one of the most debilitating complications. In this study, the hypoglycemic effect and the preventive effect of diabetic complications of Dioscorea rhizoma extract(DRE) were examined in rodent model. We investigated the glucose tolerance test and long term hypoglycemic effect of DRE in Type 1 streptozotocin-induced diabetic rats and Type 2 diabetic db/db mice. DRE showed a hypoglycemic effect on blood glucose levels than that of control group in Type 1 streptozotocin-induced diabetic rats and Type 2 diabetic db/db mice. On the basis of our results, we conclude that long-term use of DRE might help decrease blood glucose level and prevention of diabetes-associated complication.

Swine Leukocyte Antigen-DQA Gene Variation and Its Association with Piglet Diarrhea in Large White, Landrace and Duroc

  • Yang, Q.L.;Kong, J.J.;Wang, D.W.;Zhao, S.G.;Gun, S.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.8
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    • pp.1065-1071
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    • 2013
  • The swine leukocyte antigen class II molecules are possibly associated with the induction of protective immunity. The study described here was to investigate the relationship between polymorphisms in exon 2 of the swine DQA gene and piglet diarrhea. This study was carried out on 425 suckling piglets from three purebred pig strains (Large White, Landrace and Duroc). The genetic diversity of exon 2 in swine DQA was detected by PCR-SSCP and sequencing analysis, eight unique SSCP patterns (AB, BB, BC, CC, CD, BD, BE and DD) representing five specific allele (A to E) sequences were detected. Sequence analysis revealed 21 nucleotide variable sites and resulting in 12 amino acid substitutions in the populations. A moderate level polymorphism and significant deviations from Hardy-Weinberg equilibrium of the genotypes distribution were observed in the populations (p<0.01). The association analysis indicated that there was a statistically significant difference in the score of piglet diarrhea between different genotypes, individuals with genotype CC showed a lower diarrhea score than genotypes AB ($0.98{\pm}0.09$), BB ($0.85{\pm}0.77$) and BC ($1.25{\pm}0.23$) (p<0.05), and significantly low than genotype BE ($1.19{\pm}0.19$) (p<0.01), CC genotype may be a most resistance genotype for piglet diarrhea.

Detection, Characterization and Antibiotic Susceptibility of Clostridioides (Clostridium) difficile in Meat Products

  • Muratoglu, Karlo;Akkaya, Esra;Hampikyan, Hamparsun;Bingol, Enver Baris;Cetin, Omer;Colak, Hilal
    • Food Science of Animal Resources
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    • v.40 no.4
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    • pp.578-587
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    • 2020
  • Clostridioides (Clostridium) difficile is a Gram (+), anaerobic, spore forming, rod shaped bacterium that can produce toxin. The objective of this study is to reveal the presence of C. difficile in meat products, to analyze the ribotype diversity by PCR and to evaluate the antibiotic susceptibility of isolated strains. The organism was isolated in 22 out of 319 (6.9%) examined meat product samples and 9 out of 22 (40.9%) isolates were identified as RT027 and all isolates had the ability of toxin production. In terms of antibiotic susceptibility, all isolates were susceptive to amoxicillin-clavulanic acid, tetracycline and vancomycin and 21 (95.4%) isolates to metronidazole. On the other hand, imipenem and cefotaxim resistance was observed in all. In conclusion, the results of this comprehensive study conducted in Turkey deduced the presence of C. difficile in different meat products. Therefore, these products can be evaluated as a potential contamination source of C. difficile from animals to humans especially for elders, youngsters, long terms wide spectrum antibiotic used and immuno-suppressed individuals.

Genomic Sequence Variability of the Prion Gene (PRNP) in Korean Cattle

  • Choi, Sang-Haeng;Chae, Sung-Hwa;Choi, Han-Ho;Kim, Jeong-Seon;Kang, Bo-Ra;Yeo, Jung-Sou;Choi, Inho;Lee, Yong-Seok;Choy, Yun-Ho;Park, Hong-Seog
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.5
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    • pp.653-660
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    • 2007
  • In this study, we have investigated sequence variants in the PRNP gene of 20 individuals belonging to the Korean cattle, and have analyzed and compared genetic features between varieties of other cattle breeds. Of the 73 sequence variants identified in Korean cattle, 27 were identified for the first time in this study, whereas 46 of these polymorphisms had previously been isolated. We discovered a 2.6 kb SNP hot spot region localized on the putative promoter region of the PRNP gene. Furthermore, the copy numbers of the octapeptide repeat (24 bp indel) which is detected on the coding sequence (CDS) of the PRNP exhibited a completely homozygous 6/6 genotype which is dominant in other cattle breeds. We also characterized a new 19 bp/10 bp allele located on the putative promoter region of the PRNP gene, which represented 0.71 in allele frequency. To the best of our knowledge, this report is the first to address polymorphisms of the PRNP gene structure in Korean cattle in which BSE has yet to be discovered. Therefore, our findings may prove useful with regard to our current understanding of allelic diversity in bovine species, and may also provide new insights into the genetic factors associated with susceptibility or resistance to BSE.