• The Journal of Korean Medicine
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• v.18 no.1
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• pp.385-398
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• 1997

The location and local arrangement of motor, sensory neurons within brain stem, nodose ganglia, spinal ganglia and sympathetic ganglia projecting to rat's kidney and meridian point BL 23, GB 25 were investigated by HRP immunohistochemical methods following injection of 5% WGA-HRP into left kidney and meridian point BL 23, GB 25. Following injection of WGA-HRP into left kidney, anterogradely labelled sensory neurons were founded within either nodose ganglia and spinal ganglia. The sensory neurons innervating rat's left kidney were observed within spinal ganglia $T_{7}{\sim}L_3$. Sympathetic motor neurons innervating rat's left kidney were labelled within left suprarenal ganglia, either celiac ganglia, superior mesenteric ganglia, and sympathetic chain ganglia $T_{1}{\sim}L_3$. Sympathetic chain ganglia were concentrated in $T_{12}{\sim}L_1$. The sensory neurons innervating rat's meridian point BL 23 were founded within spinal ganglia $T_{2}{\sim}L_2$. They were numerous in spinal in ganglia $T_{10}{\sim}T_{12}$. Sympathetic motor neurons innervating rat's meridian point BL 23 were observed in suprarenal ganglia and greater splanchnic trunk, sympathetic chain ganglia from $T_1$ to $L_3$. They were concentrated in $T_{12}{\sim}L_3$. The sensory neurons innervating rat's meridian point GB 25 were labelled within spinal ganglia $T_{6}{\sim}T_{13}$. They were numerous in from T10 to $T_{12}$. Sympathetic motor neurons innervating rat's meridian point GB 25 were labelled within greater splanchnic trunk and sympathetic chain ganglia $T_{12}{\sim}L_3$. They were concentrated in $T_{13}{\sim}L_1$. This results neuroanatomically imply that the location of rat's motor and sensory neurons innervating meridian point BL 23 and GB 25 were closely related that of innervating kidney.

• Biomolecules & Therapeutics
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• v.30 no.5
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• pp.418-426
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• 2022

Chimeric antigen receptor T (CAR-T) cell therapy is one of the promising anticancer treatments. It shows a high overall response rate with complete response to blood cancer. However, there is a limitation to solid tumor treatment. Additionally, this currently approved therapy exhibits side effects such as cytokine release syndrome and neurotoxicity. Alternatively, bispecific antibody is an innovative therapeutic tool that simultaneously engages specific immune cells to disease-related target cells. Since programmed death ligand 1 (PD-L1) is an immune checkpoint molecule highly expressed in some cancer cells, in the current study, we generated αCD3xαPD-L1 bispecific antibody (BiTE) which can engage T cells to PD-L1⁺ cancer cells. We observed that the BiTE-bound OT-1 T cells effectively killed cancer cells in vitro and in vivo. They substantially increased the recruitment of effector memory CD8⁺ T cells having CD8⁺CD44⁺CD62L^low phenotype in tumor. Interestingly, we also observed that BiTE-bound polyclonal T cells showed highly efficacious tumor killing activity in vivo in comparison with the direct intravenous treatment of bispecific antibody, suggesting that PD-L1-directed migration and engagement of activated T cells might increase cancer cell killing. Additionally, BiTE-bound CAR-T cells which targets human Her-2/neu exhibited enhanced killing effect on Her-2-expressing cancer cells in vivo, suggesting that this could be a novel therapeutic regimen. Collectively, our results suggested that engaging activated T cells with cancer cells using αCD3xαPD-L1 BiTE could be an innovative next generation anticancer therapy which exerts simultaneous inhibitory functions on PD-L1 as well as increasing the infiltration of activated T cells having effector memory phenotype in tumor site.

• Herbal Formula Science
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• v.14 no.2
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• pp.86-96
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• 2006

Objectives : This study wad designed to elucidate the short term effect of Rossa rugosae Radix on proliferation, differentiation and maturation of 3T3-L1 Preadipocyte. Methods : 3T3-L1 preadipocytes obtained from Korean Cell line Bank were cultured in a Dulbecco's modified eagle medium(DMED) culture colution containing 10% fetal bovine serum(FBS) and various concentration of aqueous extract of Rossa rugosae Radix on proliferation, differentiation and maturation of 3T3-L1 preadipocytes were investigate after treatment for 24 hours b measuring MTT, Oil Red O and latate dehydrogenase activity.. Results : The Rossa rugosae Radix extract inhibited significantly the proliferation of 3T3-L1 preadipocytes and tended to increase latate dehydrogenase activity in the media of differentiated 3T3-L1 preadipocytes & matured 3T3-L1 preadipocytes. the extract also inhibit the lipid accumulation of differentiated and maturaion of 3T3-L1 preadipocytes, suggesting that Rossa rugosae Radix has anti-obesity effect: however further in vivo study is needed to demonstrate its pharmacological effects.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.8
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• pp.1575-1580
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• 2012

In this paper, a DVB-T2(Digital Video Broadcasting-the 2nd Generation Terrestrial) receiver is designed under a USB-type windows environment, and the baseband frames for MPEG2-TS stream(File or Ethernet Modes) are analyzed for verifying the receiver. In addition, the performance of the BICM(Bit Interleaved Coding & Modulation) module in the receiver is analyzed in terms of PLP(Physical Layer Pipe) and L1(Layer 1) signals.

• Molecules and Cells
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• v.40 no.1
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• pp.24-36
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• 2017

The stability of peptide-MHC complex (pMHC) is an important factor to shape the fate of peptide-specific T cell immune response, but how it influences on T cell activation process is poorly understood. To better understand that, we investigated various T cell activation events driven by $L^d$ MHCI loaded with graded concentrations of P2Ca and QL9 peptides, respectively, with 2C TCR Tg T cells; the binding strength of P2Ca for $L^d$ is measurably weaker than that of QL9, but either peptides in the context of $L^d$ interact with 2C TCR with a similar strength. When their concentrations required for early T cell activation events, which occur within several minutes to an hour, were concerned, $EC_{50}s$ of QL9 were about 100 folds lower than those of P2Ca, which was expected from their association constants for $L^d$. When $EC_{50}s$ for late activation events, which takes over several hours to occur, were concerned, the differences grew even larger (> 300 folds), suggesting that, due to weak binding, $L^d/P2Ca$ dissociate from each other more easily to lose its antigenicity in a short time. Accordingly, fixation of $L^d/P2Ca$ with paraformaldehyde resulted in a significant improvement in its immunogenicity. These results imply that binding strength of a peptide for a MHC is a critical factor to determine the duration of pMHC-mediated T cell activation and thus the attainment of productive T cell activation. It is also suggested that paraformaldehyde fixation should be an effective tool to ameliorate the immunogenicity of pMHC with a poor stability.

• Honam Mathematical Journal
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• v.39 no.1
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• pp.93-100
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• 2017

Let $\mathcal{H}$ be an infinite dimensional separable Hilbert space with a fixed orthonormal base $\{e_1,e_2,{\cdots}\}$. Let $\mathcal{L}$ be the subspace lattice generated by the subspaces $\{[e_1],[e_1,e_2],[e_1,e_2,e_3],{\cdots}\}$ and let $Alg{\mathcal{L}}$ be the algebra of bounded operators which leave invariant all projections in $\mathcal{L}$. Let p and q be natural numbers($p{\leqslant}q$). Let $\mathcal{B}_{p,q}=\{T{\in}Alg\mathcal{L}{\mid}T_{(p,q)}=0\}$. Let $\mathcal{A}$ be a linear manifold in $Alg{\mathcal{L}}$ such that $\{0\}{\varsubsetneq}{\mathcal{A}}{\subset}{\mathcal{B}}_{p,q}$. If $\mathcal{A}$ is an ideal in $Alg{\mathcal{L}}$, then $T_{(i,j)}=0$, $p{\leqslant}i{\leqslant}q$ and $i{\leqslant}j{\leqslant}q$ for all T in $\mathcal{A}$.

• Communications of the Korean Mathematical Society
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• v.12 no.3
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• pp.597-602
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• 1997

Let H be a separable complex Hilbert space and L(H) be the *-algebra of all bounded linear operators on H. For $T \in L(H)$, we construct a pair of semi-positive definite operators $$ $\mid$T$\mid$_r = (T^*T)^{\frac{1}{2}} and $\mid$T$\mid$_l = (TT^*)^{\frac{1}{2}}. $$ An operator T is called a semi-hyponormal operator if $$ Q_T = $\mid$T$\mid$_r - $\mid$T$\mid$_l \geq 0. $$ In this paper, by using a technique introduced by Berberian [1], we show that the approximate point spectrum $\sigma_{ap}(T)$ of a semi-hyponomal operator T is empty.

• The Korean Journal of Malacology
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• v.17 no.1
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• pp.7-12
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• 2001

The age and growth of the short-necked clam, Paphia undalata, was investigated from 546 samples randomly collected in December 2000 in Kwangyang Bay, Korea. Ages were determined from ring radius of shell and the maximum age was observed to be 2 years. The relationship between shell length (SL) and shell height (SH) of Paphia undalata was SL = 0.2105 ＋ 1.7569 $\times$ SH ($R^2$= 0.98), and the shell length (SL)-total weight (TW) relationship was TW = 2.5824 $\times$ 10$^{-4}$ $\times$ S $L^{2.6769}$ ($R^2$= 0.92). The von Bertalanffy growth parameters were estimated by the non-linear method, with values as follows: $L_{\infty}$ = 81.46 mm, K : 0.20/year, $t_{0}$ = -1.19 year. The von Bertalnanffy growth equation was $L_{t}$ = 81.46(1- $e^{-0}$.20(t+1.19)/), $W_{t}$ = 33.68(1- $e^{-0}$.20(t+1.19)/)$^{2.6769}$.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.1 s.32
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• pp.169-176
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• 2007

Objectives : The purpose of this study was to investigate inhibitory effect of Gagamtongsung-San(GTS) on the cancer. Methods : This study estimated the cytotoxicity of GTS about L1210 and NIH3T3. We used GTS extract distilled with water, n-Hexane, Ethyl acetate and Butanol. The cytotoxicitys of GTS about cancer cells and normal cells were tested using a colorimetric tetrazoliun assay(MTT assay). Results : The results of this study were obtained as follow ; l. Cytotoxicity of water extract of GTS in L1210 cell lines was significantly increased, compared with NIH3T3. 2. n-Hexane fraction of GTS had similar cytotoxicity between L1210 and NIH3T3, and that have similar $IC_{50}$ of water extract of GTS at 276 ${\mu}g/ml$ 3. Ethyl acetate fraction of GTS had low degree cytotoxicity both L1210 and NIH3T3 cell lines. 4. Butanol fraction of GTS had cytotoxicity between L1210 and NIH3T3. Significantly, Cytotoxicity of GTS in L1210 cell lines was significant increased. 5. $H_2O$ fraction of GTS had no cytotoxicity both L1210 and NIH3T3.

• Journal of applied mathematics & informatics
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• v.28 no.3_4
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• pp.1003-1008
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• 2010

In allelic model $X\;=\;(x_1,\;x_2,\;\cdots,\;x_d)$, $$M_f(t)\;=\;f(p(t))\;-\;\int_0^t\;Lf(p(t))ds$$ is a P-martingale for diffusion operator L under the certain conditions. In this note, we define $T_tf\;=\;E_{p_0}^{p^*}\;[f((P(t))]$ for $t\;{\geq}\;0$ for using a new diffusion operator $L^*$ and we show the diffusion relations between $T_t$ and diffusion operator $L^*$.