• 한국농촌건축학회논문집
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• 제21권4호
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• pp.45-52
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• 2019

Chemical grouting method is mainly used for construction of dams and reservoirs, stabilization and reinforcement of slopes, reinforcement of soft grounds such as embankments, dredging and landfills, the order of earthquake response method, and the reinforcement of structures. Recently, it is widely applied in construction sites such as highways, airfields, high-speed railways, subsea facilities, port construction works, tunnels, and subway works. As such, the demand for grouting continues to increase. The development of the grouting method was focused on increasing the strength of the ground, and the development of the chemical additives, the injection device, and the stirring device were mainly performed. But ordinary portland cement used for grouting is a product that consumes natural resources such as limestone, generates a large amount of greenhouse gases, consumes a large amount of energy sources, and it is time to develop products and new methods to replace them. In this study, Ordinary Portland Cement and New Grouting Binder (circulating fluidized bed boiler fly and blast furnace slag) were compared and analyzed by the following test. Homo-gel strength and homo-gel time, water quality analysis of the water used and soil contamination process tests of homo-gel samples were performed. In the case of NGB, when Using water is used as the reservoir water, the strength measured smaller than that of the other water. However, it shows about 2.5 times greater than the homo-gel compressive strength applied to OPC (7-day, reservoir water), so there is no problem with water quality when applied.

• 한국지반공학회논문집
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• 제26권9호
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• pp.47-58
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• 2010

본 논문에서는 2.0 Shot 방식의 최적 주입비를 산정하고 이에 대한 주입시간과 강도에 대한 검증을 실시하였다. 따라서 본 연구를 수행하기 위하여 유제거동 지배방정식을 이용하여 최적 주입비를 산정하고 이에 대한 실내실험과 현장실험을 실시하였다. 실내실험은 주입비의 변화에 따른 주입시간의 비교, 재령과 공극비의 변화에 따른 강도 특성을 예측하기 위한 호모겔과 샌드겔의 강도실험으로 구분하여 실시하였다. 연구결과 2.0 Shot 방식의 최적 주입비는 1:2 일 경우 침투가 가장 효율적으로 나타났다. 강도 실험에서도 다른 주입비에 비해 최적 주입비가 큰 강도를 발현하는 것으로 나타났다. 또한 현장실험을 통하여 현장에서도 높은 강도가 발현될 것으로 판단된다.

• Applied Biological Chemistry
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• 제47권2호
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• pp.279-282
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• 2004

An antioxidant was isolated from Korean mistletoe (Viscum album var. coloratum) by consecutive purification using silica gel, Sephadex LH-20, and RP-HPLC. The active principle was identified as homo-flavoyadorinin-B (3',7-dimethoxyluteolin-4'-O-[apiosyl $(1{\rightarrow}2)$ glucoside]) by spectral analyses. It inhibited 74.6% of hydroxyl radical and 30.6% of superoxide anion radical at 0.01 mM; however, th~compound did not show any scavenging activity against hydrogen peroxide radical. At 0.1 mM, above compound scavenged superoxide anion radical about twice as effective as positive controls, BHT and ${\alpha}-tocopherol$. Radical scavenging activities of homo-flavoyadorinin-B on DPPH, hydroxyl, and hydrogen peroxide radicals were almost same with those of positive controls.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2003년도 봄 학술발표회 논문집
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• pp.689-696
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• 2003

In this study, ordinary portland cement, slag cement and micro cement which have been used in the construction fields were evaluated for the environmental effects and compression strength characteristics for curing solution. To find the leaching of C $r^{6+}$ characteristics in cement grouts, C $r^{6+}$ content tests were performed for the raw materials(cement powder). In addition, C $r^{6+}$ leaching tests were peformed for the homo-gel samples according to change of pH and each curing solution with the deionized water and leachate. Then, the unconfined compression strength tests were peformed with the homo-gel samples and the amount of changed C $r^{6+}$ was measured by curing solution.

• 한국지반환경공학회 논문집
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• 제11권9호
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• pp.47-53
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• 2010

본 연구는 최근 개발된 실리카졸 그라우트재의 압축강도 특성을 규명한 것으로써 연구에 사용된 그라우트재는 3차원 겔형성재인 실리카졸 그라우트재와 규산나트륨 그라우트재에 대한 공학적 특성을 비교하기 위하여 일축압축강도시험(호모겔, 샌드겔), 투수시험, 휨강도시험등을 통해 강도 특성과 친환경성을 규명하고자 하였다. 실리카졸 그라우트재가 규산나트륨 그라우트재보다 초기(72시간 이내)에 일축압축강도가 3~5배 이상의 고강도로 증진되고 있음을 확인하였다. 샌드겔과 호모겔의 재령 28일의 일축압축강도로 비교 시 실리카졸 그라우팅제의 경우 샌드겔이 호모겔에 비해 약 1.3배 정도 강도가 높게 측정되었고, 이는 실리카졸의 경우 실제로 지반에 주입되어 사용되었을 경우 고강도를 발휘하는 특성이 있기 때문으로 추정된다. 투수시험 결과 실리카졸이 차수성면에서 현장에 적용하는데 무리가 없다고 판단되어 차수성을 요구하는 현장에 적용이 가능하다고 판단된다. 휨강도시험결과 그라우팅제용 실리카졸은 최종 28일 강도의 경우 규산나트륨 그라우팅제에 비해 3배 이상의 차이를 보이고 있다.

• 한국건축시공학회지
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• 제16권1호
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• pp.45-52
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• 2016

본 연구에서는 무시멘트 결합재(NCB)를 사용한 지반 그라우팅용 약액주입재의 B액 결합재의 종류, 결합재의 W/B 및 A액과 B액의 부피비에 따른 겔타임 및 호모겔강도의 변화 특성을 검토함으로써 무시멘트 결합재의 지반 그라우팅용 결합재로서의 적용 가능성을 검토하고자 하였다. 무시멘트 무기결합재는 당사에서 공동연구를 통해 개발된 시멘트를 대체할 수 있는 고로슬래그 기반의 친환경 무기결합재로서 화학조성비가 다른 NCB-1, 2, 3을 사용하여 실험을 진행하였다. 실험 및 현장 적용성 분석 결과, 겔타임 및 호모겔강도의 요구성능을 확보할 수 있는 결합재 종류별 W/B 및 A액과 B액의 부피비가 도출되었으며, W/B가 100~140%, A : B가 50 : 50~30 : 70인 조건에서 급결구간 및 중결구간의 경우 NCB가 OPC 대비 동일한 겔타임 수준에서 호모겔강도의 확보가 유리한 것으로 평가되어 NCB의 지반 그라우팅용 결합재로서의 적용이 가능할 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1666-1673
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• 2011

The presence of sialoglycoproteins (SGPs) in the membranes from goat (Capra aegagrus hircus), buffalo (Bubalus bubalis bubalis) and pig (Sus scrofa domestica) erythrocytes was investigated by partial purification with a chloroform-methanol extraction method followed by Sodium dodecyl sulphate - Polyacrylamide gel electrophoresis in comparison to human (Homo sapiens) erythrocytes. The results show that mammalian erythrocytes possess clear differences in the SGPs numbers and molecular weights although all animals studied in this experiment are from the same class i.e. mammalia. The SGPs number in human, goat, buffalo and pig are four (PAS-1 to PAS-4), ten (PAS-GI to PAS-GX), seven (PAS-BI to PAS-BVII) and four (PAS-PI to PAS-IV) respectively as indicated by staining the polyacrylamide gel with sialoglycoprotein-specific Periodic acid-Schiff's (PAS) stain. The new SGPs could be observed only after the partial purification of membrane fractions named as PAS-HI with molecular weight (Mr) 190 kDa and PAS-HII 150 kDa in human, PAS-BIA in buffalo and PAS-PIA and PAS-PIVA in pig. The gels were also stained with Coomassie brilliant blue (CBB) and Silver stain to check the contamination of other membrane proteins in the purified fractions. The quantitative distribution of SGPs was also determined by densitometry. Present study indicates that there are some basic differences in mammalian erythrocyte membrane SGPs, especially with respect to their number and molecular weights indicating major structural variations.

• Geomechanics and Engineering
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• 제19권4호
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• pp.361-368
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• 2019

This study aims to evaluate the applicability of a grout material that is mixed with carbon fiber, biogrout, ground granulated blast furnace slag (GGBS) powder and cement. Uniaxial compressive strength tests were performed on homo-gel samples at days of 1, 3, 7, 14 and 28. In addition, the variation of permeability with the mixing ratios was measured. Based on the uniaxial compressive strength test, it was confirmed that the uniaxial compressive strength increased by 1.2times when carbon fiber increased by 1%. In addition, as a result of the permeability test, it was found that when the GGBS increased by 20%, the permeability coefficient decreased by about 1.5times. Therefore, the developed grout material can be used as a cutoff grouting material in the field due to its strength and cut-off effect.

• 한국가축번식학회지
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• 제19권3호
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• pp.235-241
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• 1995

These experiments were carried out to find genetic polymorphisms of Serum protein like Pre-albumin(Pr), Albumin(Al) and Transferrin(Tf), and establish preservation of pure pedigree in Korean Native Goats(KNG). Their serum was collected and examined from the total of 74 KNG that raised in Tang Jin district, Chungnam-province. They were biochemically analysed by polyacrylamide gel(7.5%) electrophoresis(PAGE) in order to estimate the frequencies of genotypes and alleles existing on each trait locus. The results obtained in these experiments were summarized as follows ; 1. In the serum Pre-albumin(Pr) locus, the frequencies of genotypes for hetero AB and homo BB observed were 55.4%, and 44.6%, respectively. While homo AA was not found in the Pr locus. The frequencies of gene in PrA and PrB were 0.723 and 0.277, respectively. Accordingly, the Pr loci were assumed to be controlled by alleles PrA and PrB. 2. The frequencies of genotypes of homo BB and hetero AB detected in Albumin(Al) locus were 75.7% and 24.3%, respectively. However, AA type was not observed in the Al locus. The gene frequencies of AlA an AlB were 0.879 and 0.121, respectively. Also, the Al loci were considered to be controlled by alleles AlA and AlB. 3. The frequencies of genotypes for hetero AD and homo DD found in Transferrin (Al) locus were 79.7% and 20.3%, respectively. Whereas, homotype AA was not detected in this locus. The gene frequencies of TfA and TfD were 0.399 and 0.601, respectively. Therefore, the serum Tf loci were assumed to be controlled by alleles Tfa and Tfd.

• Journal of Plant Biology
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• 제39권1호
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• pp.41-48
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• 1996

발아 후 8일된 해녀콩 자엽에서 성질이 서로 다른 두 개의 homoserine dehydrogenase를 분리하였다. 자엽에서 얻은 조효소액을 열처리, 황산 암모늄 침전, DEAE-Sephacel 및 Sephacryl S-300 겔 크로마토그래피와 Procion red dye, Cibacron blue dye 및 Resource Q 컬럼 크로마토그래피로 정제하였다. 겔 크로마토그래피에서 얻은 2개의 활성분획 중 T-형(트레오닌 감수성)과 K-형(트레오닌 비감수성)의 분자량은 각각 230 kD과 135 kD이었다. 10mM 트레오닌 첨가로 T-형 효소는 70% 이상의 활성저해를 받았으나 K-형 효소는 전혀 억제를 받지 않았다. Homoserine에 대한 Km은 T-형이 1.6mM, K-형이 0.3mM이었고, NAD에 대한 Km은 T-형이 2.34mM, K-형이 0.03mM이었으며 NADP에 대한 Km은 두 효소에서 동일하게 0.01mM로 산출되었다. 400mM KCl에서 T-형은 4.9배, K-형은 2.8배의 활성증가를 보였다. 부분정체(Sephacryl S-300 겔 크로마토그래피)된 상태의 T-형과 K-형은 조건에 따라 쉽게 상호전환되었다.