• 농촌계획
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• 제20권1호
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• pp.161-173
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• 2014

This study aimed to adaptive reuse plan a take advantage of Suncheon Bay Garden Expo 2013. Detailed objectives included: to identify and foster cultural and ecological resources in Suncheon Bay and Garden Expo Site; and to search and propose characterized program in the site for efficient utilization; and to revitalize a site for the vitalizations of the region where garden culture and garden industries for strengthening local competitiveness. The study was multi-phased. The first stage included basic surveys such as site status and environmental context analysis, and questionnaires and similar case studies, and the second stage was to review the direction of preliminary planning, develop associated industries and strategies for regional revitalization and review and incorporate project details. And the third stage aimed to develop a comprehensive plan from spatial plans and program plans and suggest plans to vitalize the operation of the site. The results of the study are as follows: First, the characterization strategy is 'healing experience tourism', 'healing garden', 'healing herbal care' are suitable for the site and regional revitalization. Second, the dimension of 2013 Suncheon Bay Garden Expo which was distinguished into four areas, in consideration of the characteristics and contents of the garden expo site for efficient operation and maintenance. Four area are as follows: the park oriented area, the experiential tourism oriented area, the existing conservation oriented area, and the area for associated industries. Third, the practice of private and public partnerships is needed in order to efficiently manage and operations. The significance of this study is that it is seeking to take advantage of the Garden Expo site and set the plan by existing resources and in consideration of local characteristics, in conjunction with area to envision and proposed management plan. Some problems are remained which have not been able to deal with details program and organization and financial planning programs for the operation of the site. This is for further study.

• 한국콘텐츠학회논문지
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• 제15권4호
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• pp.114-121
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• 2015

이 연구는 사회복지사의 경험적 인식을 현상학적으로 접근하여 노인무용의 교육효과와 이에 따른 치유작용 메커니즘을 밝히는데 목적이 있다. 연구에 참여한 사회복지사는 2014년도 한국문화예술교육진흥원 사회복지 문화예술교육 지원사업에서 무용분야 수혜기관으로 선정된 노인복지기관의 사회복지사 7명이다. 연구의 자료는 1:1 심층면담을 통해 수집하였다. 연구결과 사복지사들은 노인무용교육을 단순한 신체활동보다는 정서, 인지, 정신, 사회적 예술활동으로 인식하고 있었다. 그리고 노인들은 무용활동으로 인해 개인의 긍정적 정서와 인지력을 자극하여 사회적 관계 속에서 지속적으로 피드백 하고 있었다. 때문에 이와 같은 과정에서 삶의 만족감이 점점 커지게 되는 눈덩이 효과(snowball effect)가 나타났다. 따라서 복지기관에서 이루어지는 노인무용 교육은 개인과 공동체간 끊임없는 상호작용 속에서 삶의 질을 점차적으로 증폭시킬 수 있는 치유적 예술활동이라 할 수 있다.

• Archives of Plastic Surgery
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• 제35권2호
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• pp.115-120
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• 2008

Purpose: Oncostatin M(OSM) is a multifunctional cytokine that belongs to the interleukin(IL)-6 family. Although there have been a number of studies that focused on the role and mechanism of OSM in various organs and tissues, there are few reports on its effect on wound healing. The final purpose of this project is to evaluate the effect of OSM on wound healing. This pilot study was designed to investigate the effect of OSM on proliferation and matrix synthesis of human dermal fibroblasts, which are the major components of the wound healing. Methods: Excess skin that was obtained from patients who underwent skin grafts, was used for this study. From this material, fibroblasts were isolated and cultured. The cultured fibroblasts were treated with one of four concentrations of OSM. The OSM concentrations used were 0, 50, 100, and 200 ng/ml, respectively. After the OSM treatment, cell proliferation was determined by the MTT assay, collagen synthesis by the C1CP method, GAG levels by the Blyscan Dye method. The parameter levels of each group were compared. Results: OSM treatment increased all the components tested in the study. In particular, cell proliferation, GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U-test). The highest increase in all the components was obtained at a 100 ng/ml concentration of OSM.Conclusion: The results of the present study indicate that OSM stimulates proliferation and matrix synthesis of human dermal fibroblast and the optimal concentration for wound healing is 100 ng/mL.

• Journal of Periodontal and Implant Science
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• 제32권4호
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• pp.753-767
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• 2002

The purpose of this study was to evaluate histologically the effect of LiF-maleic acid added calcium aluminate(LM-CA) bone cement & CA-PMMA composite bone cement on the healing of calvarial defect in Sprague-Dawley rats. The critical size defects were surgically produced in the calvarial bone using the 8mm trephine bur. The rats were divided in three groups : In the control group, nothing was applied into the defect of each rat. LM-CA bone cement was implanted in the experimental group 1 and CA-PMMA composite bone cement was implanted in the experimental group 2. Rats were sacrificed at 2, 8 weeks after surgical procedure. The specimens were examined by histologic analysis, especially about the bone-cement interface and the response of surrounding tissue. The results are as follows; 1. In the control group, inflammatory infiltration was observed at 2 weeks. At 8 weeks, periosteum and duramater were continuously joined together in the defect area. But the center of defect area was filled up with the loose connective tissue. 2. In the experimental group 1, the bonding between implanted bone cement and the existing bone was seen, which more increased in 8 weeks than 2 weeks. Inflammatory infiltration and the dispersion of implanted bone cement particles were seen in both 2 weeks and 8 weeks. 3. In the experimental group 2, implanted bone itself had a dimensional stability and no bonding between implanted bone cement and the existing bone was seen in both 2 weeks and 8 weeks. Implanted bone cement was encapsulated by fibrous connective tissue. In addition, inflammatory infiltration was seen around implanted bone cement. On the basis of these results, when LM-CA bone cement or CA-PMMA composite bone cement was implanted in rat calvarial defect, LM-CA bone cement can be used as a bioactive bone graft material due to ability of bonding to the existing bone and CA-PMMA can be used as a graft material for augmentation of bone-volume due to dimensional stability.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권2호
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• pp.130-135
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• 2013

Primary intraosseous squamous cell carcinoma (PIOSCC) is a rare form arising within the jaws. PIOSCC is not related to the oral mucosa, presumably developing from remnants of the odontogenic epithelium. Because odontogenic cyst epithelium often transforms malignantly into PIOSCC, it could be misdiagnosed as odontogenic cyst based on a relatively ill-defined radiolucent lesion. Therefore, definite diagnosis is established from histological examination of biopsy samples taken during cyst enucleation in many cases. The present study is reported with a case of patient complaining of discomfort on his mandible. He was diagnosed as a putative dentigerous cyst and underwent a cyst enucleation treatment. After definite diagnosis as PIOSCC was established based on histologic findings, partial mandible resection and mandible reconstruction were performed. Up to the present, 10 months follow up of the patient showed satisfactory healing without recurrence and abnormal findings; thereby, we are reporting this case with literature review.

• 한국임상수의학회지
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• 제35권4호
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• pp.137-140
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• 2018

A five-month-old toy poodle was referred for treatment of bilateral mandibular fractures. Preoperative skull and dental radiographs showed rostral mandibular fractures and permanent teeth that had not yet erupted. In addition to providing stability for the healing of fractures, it is very important to focus on restoring normal occlusion. The mandibular fractures were repaired using interdental wiring in a dentate area where the deciduous canines and fourth premolars were stable and could be used to anchor the interdental fixation. In the absence of an erupted permanent mandibular first molar, the modified Risdon wiring was performed using the deciduous mandibular fourth premolars as anchor teeth. An intraoral splint was placed on the mandibular teeth using self-curing composite resin. Five months after surgery, the patient had experienced no complications, and the permanent teeth had erupted normally. In this case, which involves a small-breed puppy with deciduous dentition, treatment with a wire-reinforced interdental splint using intact deciduous fourth premolar teeth as anchor teeth can be considered as a suitable method for rostral mandibular fracture restoration.

• IMMUNE NETWORK
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• 제21권4호
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• pp.30.1-30.12
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• 2021

High expression of mitofusin-2 (MFN2), a mitochondrial fusion protein, has been frequently associated with poor prognosis of patients with cervical cancer. Here, we aimed to identify the function of MFN2 in cervical cancer to understand its influence on disease prognosis. To this end, from cervical adenocarcinoma, we performed an MTT assay and quantitative RT-PCR (qRT-PCR) analysis to assess the effects of MFN2 on the proliferation and of HeLa cells. Then, colony-formation ability and tumorigenesis were evaluated using a tumor xenograft mouse model. The migration ability related to MFN2 was also measured using a wound healing assay. Consequently, epithelial-mesenchymal transition (EMT) of MFN2-knockdowned HeLa cells originating from adenocarcinoma. markers related to MFN2 were assessed by qRT-PCR. Clinical data were analyzed using cBioPortal and The Cancer Genome Atlas. We found that MFN2 knockdown reduced the proliferation, colony formation ability, migration, and in vivo tumorigenesis of HeLa cells. Primarily, migration of MFN2-knockdowned HeLa cells decreased through the suppression of EMT. Thus, we concluded that MFN2 facilitates cancer progression and in vivo tumorigenesis in HeLa cells. These findings suggest that MFN2 could be a novel target to regulate the EMT program and tumorigenic potential in HeLa cells and might serve as a therapeutic target for cervical cancer. Taken together, this study is expected to contribute to the treatment of patients with cervical cancer.

• Journal of Periodontal and Implant Science
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• 제49권4호
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• pp.228-236
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• 2019

Purpose: The purpose of this study was to evaluate the synergistic effect of adjunctive hyperbaric oxygen (HBO) therapy on new bone formation and angiogenesis after 8 weeks of healing. Methods: Sprague-Dawley rats (n=28) were split into 2 groups according to the application of adjunctive HBO therapy: a group that received HBO therapy (HBO group [n=14]) and another group that did not receive HBO therapy (NHBO group [n=14]). Each group was divided into 2 subgroups according to the type of bone graft material: a biphasic calcium phosphate (BCP) subgroup and an Escherichia coli-derived recombinant human bone morphogenetic protein-2-/epigallocatechin-3-gallate-coated BCP (mBCP) subgroup. Two identical circular defects with a 6-mm diameter were made in the right and left parietal bones of each rat. One defect was grafted with bone graft material (BCP or mBCP). The other defect was not grafted. The HBO group received 2 weeks of adjunctive HBO therapy (1 hour, 5 times a week). The rats were euthanized 8 weeks after surgery. The specimens were prepared for histologic analysis. Results: New bone (%) was higher in the NHBO-mBCP group than in the NHBO-BCP and control groups (P<0.05). Blood vessel count (%) and vascular endothelial growth factor staining (%) were higher in the HBO-mBCP group than in the NHBO-mBCP group (P<0.05). Conclusions: HBO therapy did not have a positive influence on bone formation irrespective of the type of bone graft material applied after 8 weeks of healing. HBO therapy had a positive effect on angiogenic activity.

• 한국산림과학회지
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• 제47권1호
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• pp.44-51
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• 1980

묘목가식(苗木假植)과 운반방법(運搬方法)을 개선하여 묘목(苗木)의 활착증대(活着增大)와 가식성력화(假植省力化)를 도모하고자 몇가지 시험(試驗)을 한 바 다음과 같은 결과(結果)를 얻었다. 1. 흑백색(黑白色)비닐빽을 사용하면 리기테다 묘목(苗木)을 35일간(日間) 창고 저장을 하여도 조림지(造林地) 활착(活看)에 영향이 없었던 것은 묘포장(苗圃場)과 조림지(造林地)에서의 토양내(土壤內) 가식(假植)을 생략(省略)할 수 있음을 증명하는 것이다. 조림용(造林用) 묘목(苗木)을 흑백색(黑白色)비닐빽에 장기저장을 할 수 있는 이유(理由)는 빽내(內) 습도(濕度)가 거의 100%이고 창고에 저장한 비닐빽내(內) 온도(溫度)가 $15^{\circ}C$ 정도에 불과하며 주야간(晝夜間)의 온도교차(溫度較差)가 거의 없기 때문이다. 이 빽은 조림용(造林用) 빽 대신에 이용할 수 있으며 조림지(造林地)에서 1~2일간(日間) 직사광선(直射光線)에 노출시킨 후 식재한 묘목(苗木)과 그늘에 저장한 묘목(苗木)의 활착간(活看間)에 차이(差異)가 없었다. 2. 묘목(苗木)을 토양내(土壤內)에 솎묶음 가식(假植)을 하는 대신에 흑백색(黑白色)비닐텐트로 피복(被覆)을 시키면 최대한 18일간(日間) 저장 한 묘(苗)와 토양내(土壤內) 가식(假植) 묘간(苗間)에 현지(現地) 활착(活着)에는 차이(差異)가 없었다. 이는 피복재료하(被覆材料下)의 습도(濕度)가 거의 95%에 가까우며 주야간(晝夜間)의 교차(較差)가 거의 없고 외기(外氣)와의 온도차(溫度差)가 크지 않기 때문으로 생각된다. 따라서 흑백색(黑白色)비닐텐트를 사용하연 묘포지(苗圃地)와 조림지(造林地)의 가식노동력(假植勞動力)을 생략(省略)할 수 있다. 3. 묘포지(苗圃地)와 조림지(造林地) 가식묘(假植苗)를 직사광선(直射光線)으로부터 보호(保獲)하고 수분손실(水分揚失)을 막아주기 위해서는 흑백색(黑白色)비밀망과 갈대발로 피복(被覆)을 시키는 것이 효과적(効果的)일 것 같다. 전자(前者)는 일사량(日射量)의 1/3을 후자(後者)는 2/3 정도를 차단시킬 수 있으며 증발량을 50%이상(以上) 억제(抑制) 시킬 수 있기 때문이다.

• Biomolecules & Therapeutics
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• 제30권2호
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• pp.126-136
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• 2022

Liver fibrosis is part of the wound healing process to help the liver recover from the injuries caused by various liver-damaging insults. However, liver fibrosis often progresses to life-threatening cirrhosis and hepatocellular carcinoma. To overcome the limitations of current in vivo liver fibrosis models for studying the pathophysiology of liver fibrosis and establishing effective treatment strategies, we developed a new mouse model of liver fibrosis using polyhexamethylene guanidine phosphate (PHMG-p), a humidifier sterilizer known to induce lung fibrosis in humans. Male C57/BL6 mice were intraperitoneally injected with PHMG-p (0.03% and 0.1%) twice a week for 5 weeks. Subsequently, liver tissues were examined histologically and RNA-sequencing was performed to evaluate the expression of key genes and pathways affected by PHMG-p. PHMG-p injection resulted in body weight loss of ~15% and worsening of physical condition. Necropsy revealed diffuse fibrotic lesions in the liver with no effect on the lungs. Histology, collagen staining, immunohistochemistry for smooth muscle actin and collagen, and polymerase chain reaction analysis of fibrotic genes revealed that PHMG-p induced liver fibrosis in the peri-central, peri-portal, and capsule regions. RNA-sequencing revealed that PHMG-p affected several pathways associated with human liver fibrosis, especially with upregulation of lumican and IRAK3, and downregulation of GSTp1 and GSTp2, which are closely involved in liver fibrosis pathogenesis. Collectively we demonstrated that the PHMG-p-induced liver fibrosis model can be employed to study human liver fibrosis.