• BMB Reports
• /
• v.30 no.5
• /
• pp.356-361
• /
• 1997

We cloned a cDNA (pPRC-1) which was comprised of 841 nucleotides from the cDNA library of a male ICR mouse submandibular gland ($SMG^+$). The nucleotide sequences of pPRC-1 were identical to those of exons 2 and 3 of the mGK-21 gene, a potentially functional glandular kallikrein identified in a Balb/c mouse, except for one nucleotide residue. Although this substitution changes Ile (ATT) in pPRC-1 to Val (GTT) in mGK-21, this difference has been explained by strain polymorphism. From the amino acid sequences predicted from its cDNA, we speculated that mGK-21 gene products/pGK21 consist of 261 amino acids including the $NH_2$-terminal signal peptide (residues 1~17), the short propeptide (residues 17~24), and the active peptide (residues 25~261). Although we did not demonstrate the enzyme activity of pGK21, it was assumed that pGK 21 was involved in the maturation of certain bioactive polypeptide(s) in mouse SMG for the following reasons : (a) mGK-21 gene was apparently expressed in a male ICR mouse SMG: (b) the proposed active site $His^{65}$, $Asp^{120}$, and $Ser^{213}$ residues were completely conserved in pGK21 just like other glandular kallikreins; (c) the cloned cDNA was translated to a predicted 27 kDa polypeptide chain in vitro: (d) the 27 kDa polypeptide chain produced by CHO cells was produced to a putative active form by trypsin.

• Biomolecules & Therapeutics
• /
• v.9 no.4
• /
• pp.277-281
• /
• 2001

Chromium is an essential nutrient and participates in glucose and lipid metabolism in human beings and animals. The present study was conducted to assess the effects of chromium picolinate (Cr-pic) on glucose tolerance and insulin sensitivity in type I and ll diabetic rats. The experimental groups were type I diabetic (streptozotocin-induced: 40 mg/kg, i.p.) and type II diabetic (Goto-Kakizaki rats) models. Each group was subdivided into control. low-dose and high-dose of Cr-pic treated groups. The Cr-pic was orally administered with Cr-pic (100 mg/kg for low dose group and 200 mg/kg for high dose group) for 4 weeks. And then we performed intraperitoneal glucose tolerance test (IPGTT) and insulin sensitivity test (ITT). The glucose tolerance test was carried out by inection of glucose (2 g/kg, i.p.). The peripheral insulin sensitivity test was con- ducted by injection of insulin (5 units/kg, s.c.) and glucose. We performed determining of blood glucose concentration at 0, 10, 30, 60, 90, and 120 min using automated glucose analyzer. The plasma insulin concentration was determined by rat insulin EIA kit. Administration of Cr-pic improved weight gain in all group s with higher significant in the low-dose group. There was no significance between the control and the Cr-pic treated groups in the area under the blood glucose curve and serum insulin concentration plots of IPGTT and peripheral ITT in type I diabetic rats. But Cr-pic treated groups showed significantly lower levels of the area under the blood glucose currie during IPGTT and ITT and the high-dose group showed less effects compared with the low-dose group in the type II diabetic rats. The plasma insulin concentration of both diabetic groups was not influenced by Cr-pic supplementation. We can conclude that chromium picolinate may improve the endogenous and exogenous insulin action and peripheral insulin sensitivity in type II diabetic rats.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.18 no.5
• /
• pp.51-58
• /
• 2014

This paper presents a fire exposure test result to evaluate fire resistance capacity of retrofit method using FRP (Fiber Reinforced Polymer) in reinforcement concrete structure. Especially, this paper focused on near-surface-mounted retrofit method; FRP is mounted into the groove after making a groove in concrete. In the test, main parameters are retrofit method and materials for fire proofing. Spray type of perlite and board type of calcium silicate were considered as external fire proof on surface while particle of calcium silicate and polymer mortar as internal one in groove. By increasing the temperature of inside heating furnace, the transfer of temperature from surface of fire proofing material to groove in specimen was measured. As a result, fire proofing using the board of calcium silicate was more effective to delay the heat transfer from outside than spraying with perlite. It was found that the fire proofing could resist outside temperature of $820^{\circ}C$ at maximum to keep the temperature of epoxy below glass transit temperature (GTT).

• Journal of the East Asian Society of Dietary Life
• /
• v.19 no.6
• /
• pp.869-881
• /
• 2009

The purpose of this study was to investigate the food habits, dietary intakes and GTTs (glucose tolerance tests) of female college students according to BMI in the Kyunggido area. Questionnaire surveys, 24 hr dietary recalls, and GTTs were conducted with 108 students (36 under-weight students, 42 normal weight students, and 30 over-weight students). The results were as follows: The under wt. and over wt. groups had lower frequencies of eating breakfast but higher frequencies of eating lunch than the normal wt. group. The ratio of students having dinner everyday was low in the order of under wt. > normal wt. > over wt.. The over wt. group skipped breakfast mainly due to lack of time and the need to get to school, and the under wt. group had a high ratio (48.1%) of lunch skipper as a result of having brunch. The over wt. subjects had poor habits of eating fast and overeating, but the under wt. students ate their meals slowly. The under wt. group had a high ratio of students who ate snacks and fast-food. All subjects wanted to immediately improve their poor habit of overeating. Plant oil, animal Fe, and vitamin E intakes were lower in the under wt. group than in the other groups. For the GTT, the over wt. students had significantly lower blood glucose levels after 30 min. than the other groups. BMI was negatively correlated with blood glucose level after 30 min, but positively correlated with crude fiber, ash, Ca, plant Ca, P, animal Fe, Na, K, $\beta$-carotene, vitamin $B_6$, niacin, and vitamin E.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.29 no.1
• /
• pp.53-60
• /
• 2016

This study discusses the thermal insulation capacity of variant of NO96 LNG (liquefied natural gas) cargo containment insulation system. Changing the insulation materials and the insulation layers of conventional GTT NO96 containment system, The thermal resistance and BOR(boil off rate) caused by the heat transfer between cryogenic and environmental temperature is discussed. Therefore, thermal analysis of LNG CCS(cargo containment system) is carried out to determine the insulation capabilities. Also, BOR is evaluated in terms of the total amount of heat invaded into CCS(cargo containment system). Variant of NO96 CCS such as NO96, NO96GW and NO96L3 membrane type during laden voyage is selected for the comparative study. Finite element model for heat transfer analysis is conducted by employing the equivalent thermal resistance model to simplify the complex insulation layers. Finally the results for each variant model are relatively compared and discussed to minimize the BOR.

• The Korean Journal of Food And Nutrition
• /
• v.30 no.4
• /
• pp.696-702
• /
• 2017

Metabolic syndrome, including obesity, glucose intolerance and elevated blood pressure, is related to type 2 diabetes and cardiovascular disease. Previous studies have reported the anti-oxidative, anti-inflammatory and anti-diabetic effects of purple corn extract. We investigated the efficacy of purple corn extract (PC) against high-fat diet (HFD)-induced obesity and glucose intolerance, and examined the underlying mechanisms by analyzing expression of proteins and genes involved in glucose regulation and macrophage infiltration. C57BL/6 mice were fed with normal chow diet (ND), or HFD treated with distilled water (DW, control) or PC, for 10 weeks. Although body weights were similar in the HFD-fed groups, we observed a decrease in the liver and epididymal adipose tissue (EAT) weights, and enhanced glucose tolerance test (GTT) results in the PC group, as compared with DW group. Liver showed increased Akt phosphorylation in the PC-treated mice; however, no changes were observed in the EAT, for all groups. In PC-treated mice, decreased macrophage infiltration was seen in the EAT, with a reduced expression of macrophage marker genes. Finally, proinflammatory cytokine gene expressions were decreased by PC in the EAT, and a modest trend for downregulation was observed in the liver. Hence, we conclude that PC may decrease glucose intolerance by increasing the phosphorylation of Akt and reducing the macrophage infiltration into the EAT.

• Archives of Pharmacal Research
• /
• v.20 no.6
• /
• pp.550-554
• /
• 1997

Chlorambucil is known to alkylate primarily N7 of guanine and N3 of adenine to induce DNA monofunctional adducts and interstrand cross-links (ISC). We have investigated the sequence specificity for DNA ISC induced by chlorambucil using duplex oligomers containing a defined cross-linkable sequences $ 5^{I}-A*TT, 5^{I}-G*TTor5^{I}-G*CC$ under bar which asterisk indicates the potential cross-linking site and underlined base indicates the potential cross-linking site on the opposite strand. An analysis of 20% denaturing polyacrylamide gel electrophoresis showed that chlorambucil was albe to induce DNA ISC in the duplex oligomers containing a sequence $5^I-GCC$. The formation of DNA ISC was not observed in the duplex oligomers containing sequences $5^I-ATT$. or $5^I-GTT$. These results indicate that chlorambucil induces guanine-guanine DNA ISC but not guanine-adenine or adenine-adenine DNA ISC. In addition, we have tested the ability of chlorambucil to induce DNA ISC within $5^I-GNNC$ or $5^I-GNNC$sequences using duplex oligomers containing the sequence$5^I-G^4G^3G^2^C$. The result of DNA strand cleavage assay showed that DNA ISC was formed at the $5^I-GGC$ sequence (an 1,3 cross-link, $G^1-G^3$) but not at $5^I-GGGC$ (an 1,4 cross-link, $G^1-G^4$) or $5^I-GC$ sequence (an 1,2 cross-link, $G^1-G^2$).

• Journal of the Architectural Institute of Korea Structure & Construction
• /
• v.33 no.12
• /
• pp.19-27
• /
• 2017

This paper is a study to improve the fire-resistance capacity of reinforced concrete (RC) members strengthened by fiber-reinforced-polymer (FRP). The fire resistance of the RC members strengthened by FRP was evaluated through high temperature exposure test. In order to improve the fire resistance of the FRP reinforcing method, a fire-proof board was attached to the reinforced FRP surface and then the high temperature exposure test was carried out to evaluate the improvement of the fire resistance performance. It was confirmed that the resistance to high temperature of NSMR could be improved somewhat compared with that of EBR from the experiment that exposed to high temperature under the load corresponding to 40% of nominal strength. When 30 mm thick fire-resistance (FR) board is attached to the FRP surface, the surface of the reinforced FRP does not reach $65^{\circ}C$, which is the glass transition temperature (GTT) of the epoxy until the external temperature reaches $480^{\circ}C$. In particular, when a high performance fire-proof mortar was first applied prior to FR board attachment, the FRP portion did not reach the epoxy glass transition temperature until the external temperature reached $600^{\circ}C$.

• Journal of Food Hygiene and Safety
• /
• v.24 no.4
• /
• pp.391-397
• /
• 2009

Carnosine is a dipeptide ($\beta$-alanyl-L-histidine) found in mammalian brain, eye, olfactory bulb and skeletal muscle at high concentrations. Its biological functions include antioxidant and anti-glycation activities. The objectives of this study were to investigate anti-diabetic effects of carnosine as determined by blood glucose levels in glucose tolerance test (GTT) and insulin tolerance test (ITT), insulin level and serum biochemical and lipid levels in male C57BL/6J db/db mice. There were five experimental groups including normal (C57BL/6J), control (vehicle), and three groups of carnosine at doses of 6, 30, and 150 mg/kg b.w. Carnosine was orally administered to the diabetic mice everyday for 8 weeks. There was no significant difference in body weight changes in carnosine-treated groups compared to the control. The treatments of carnosine significantly decreased the blood glucose level in the diabetic mice compared with the control (p < 0.05) after 5 weeks. The treatments of carnosine also significantly decreased the blood glucose levels in GTT and ITT and glycosylated hemoglobin (HbA1c), compared with the control (p < 0.05). Carnosine at the dose of 6 mg/kg significantly decreased the serum insulin level compared to the control (p < 0.05). Carnosine significantly increased total proteins but significantly decreased lactate dehydrogenase and blood urea nitrogen compared with the control (p < 0.05). Carnosine also significantly decreased glucose, LDL, and triglyceride in the serum of diabetic mice compared to the control (p < 0.05). These results suggest that carnosine has a hypoglycermic effect resulting from reduction of glucose and lipid levels and that high carnosine-containing diets or drugs may give a benefit for controlling diabetes mellitus in humans.

• Journal of the Korean Electrochemical Society
• /
• v.24 no.3
• /
• pp.52-64
• /
• 2021

The effect of Fe and BO₃ doping on structure, thermal, and electrical properties of Li_1+xFe_xTi_2-x(PO₄)_3-y(BO₃)_y (x = 0.2, 0.5)-based glass and glass ceramics was investigated. In addition, their crystallization behavior during sintering and ionic conductivity were also investigated in terms of sintering temperature. FT-IR and XPS results indicated that Fe²⁺ and Fe³⁺ ions in Li_1+xFe_xTi_2-x(PO₄)_3-y(BO₃)_y glass worked as a network modifier (FeO₆ octahedra) and also as a network former (FeO₄ tetrahedra). In the case of the glass with low substitution of BO₃, boron formed (PB)O₄ network structure, while boron preferred BO₃ triangles or B₃O₃ boroxol rings with increasing the BO₃ content owing to boic oxide anomaly, which can result in an increased non-bridging oxygen. The glass transition temperature (GTT) and crystallization temperature (CT) was lowered as the BO₃ substitution was increased, while Fe²⁺ lowered the GTT and raised the CT. The ionic conductivity of Li_1+xFe_xTi_2-x(PO₄)_3-y(BO₃)_y glass ceramics were 8.85×10^-4 and 1.38×10^-4S/cm for x = 0.2 and 0.5, respectively. The oxidation state of doped Fe and boric oxide anomaly were due to the enhanced lithium ion conductivity of glass ceramics.