Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Antiglycemic Effect of Carnosine in Diabetic Mice

당뇨 마우스에서 카르노신의 혈당강하 효과

  • Hue, Jin-Joo (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Jong-Soo (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Jun-Hyeong (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Nam, Sang-Yoon (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Yun, Young-Won (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Jeong, Jae-Hwang (Department of Biotechnology and Biomedicine, Chungbuk Provincial University of Science and Technology) ;
  • Lee, Beom-Jun (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Published : 2009.12.31
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Abstract

Carnosine is a dipeptide ($\beta$-alanyl-L-histidine) found in mammalian brain, eye, olfactory bulb and skeletal muscle at high concentrations. Its biological functions include antioxidant and anti-glycation activities. The objectives of this study were to investigate anti-diabetic effects of carnosine as determined by blood glucose levels in glucose tolerance test (GTT) and insulin tolerance test (ITT), insulin level and serum biochemical and lipid levels in male C57BL/6J db/db mice. There were five experimental groups including normal (C57BL/6J), control (vehicle), and three groups of carnosine at doses of 6, 30, and 150 mg/kg b.w. Carnosine was orally administered to the diabetic mice everyday for 8 weeks. There was no significant difference in body weight changes in carnosine-treated groups compared to the control. The treatments of carnosine significantly decreased the blood glucose level in the diabetic mice compared with the control (p < 0.05) after 5 weeks. The treatments of carnosine also significantly decreased the blood glucose levels in GTT and ITT and glycosylated hemoglobin (HbA1c), compared with the control (p < 0.05). Carnosine at the dose of 6 mg/kg significantly decreased the serum insulin level compared to the control (p < 0.05). Carnosine significantly increased total proteins but significantly decreased lactate dehydrogenase and blood urea nitrogen compared with the control (p < 0.05). Carnosine also significantly decreased glucose, LDL, and triglyceride in the serum of diabetic mice compared to the control (p < 0.05). These results suggest that carnosine has a hypoglycermic effect resulting from reduction of glucose and lipid levels and that high carnosine-containing diets or drugs may give a benefit for controlling diabetes mellitus in humans.

당뇨는 비정상적 인슐린의 작용으로 고혈당상태에 이르게 되는 내분비계 질환으로서, 다양한 합병증을 야기는 질환이다. carnosine은 $\beta$-alanine과 L-histidine으로 이루어진 dipeptide로서 생체내 여러 조직에 널리 분포하고 있고, 항노화, 항산화 기능을 가지는 것으로 알려져 있다. 본 연구에서는 제2형 C57BL/6J db/db mice모델을 사용하여 8주 동안 carnosine 투여 후 carnsoine의 항당뇨 효과를 보았다. 실험군은 총 5개로 정상군, 대조군, carnosine 6, 30, 150 mg/kg 투여군으로 설정하였다. 혈당조절정도를 마우스의 정맥혈에서 혈당측정기를 이용하여 측정하였고, 포도당 내성 검사를 통해 시험물질의 포도당 의존성을 보았고, 또한 혈청내의 생화학적수치와 지질학적수치 등을 측정하여 증감의 정도를 측정하였다. 결과로서, carnosine 투여는 8주후 혈당을 유의적으로 감소시켰으며(p < 0.05), 혈당내성 검사 및 인슐린내성검사에서도 유의적으로 혈당치를 낮추었다(p < 0.05), 혈중 인슐린 량은 carnsoine 투여군 모두에서 낮게 나타났으나, 특히 저농도에서 유의적으로 낮았다 (p < 0.05). 혈액생화학적 수치에서는 carnosine 투여 군이 대조군보다 총단백질 수치가 높았으며, LDH 및 BUN 수치는 낮게 나타났다. 혈액 지질수치에서도 carnosine투여는 glucose 농도를 유의적으로 감소시켰으며, 더불어 LDL 수치를 낮췄으나, HDL 수치에는 변화가 없었다. 결론적으로 carnosine은 제2형 마우스모델에서 농도 비의존적 항당뇨 효과를 나타내었으나, carnosine는 생체내 항산화 및 항노화 효과와 더불어 항당뇨 효과를 갖는 것으로 사료된다.

Keywords

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