• Journal of Species Research
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• 제11권3호
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• pp.162-168
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• 2022

Compared to marine dinoflagellates, freshwater species are rarely recorded in Korea. In the present study, we isolated a freshwater dinoflagellate, Palatinus, from the Paldang Reservoir, Korea, in December 2021. The overall cell shape was ovoid, and the cell size was 34.3 ㎛ in length (25.8-39.5 ㎛) and 28.4 ㎛ in width (21.5-34 ㎛). An eyespot was usually observed near the sulcal region. The Kofoidian plate formula of the species was determined to be 4', 2a, 7", 6c, 5s, 5''', and 2''''. Apical pore complex was not observed. However, variations in the cingular plate caused by the fusion of 3C and 4C were observed. Analyses of 28S rDNA sequences revealed that the unidentified species is 100% similar to Palatinus apiculatus, and clustered together in the same lineage in the phylogenetic tree (100% bootstrap value). Our findings confirmed that the isolated dinoflagellate is Palatinus apiculatus, which was discovered for the first time in Korean freshwaters.

• 디지털융복합연구
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• 제20권5호
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• pp.275-280
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• 2022

본 논문은 비대면 미술감상 교육을 위해 사용자의 상황 정보 및 취향에 맞는 태그가 융합된 융·복합 예술 플레이리스트 콘텐츠를 사용자에게 추천하는 기술을 제안하고자 한다. 제안한 기술의 구현을 위해 예술 작품의 특성을 분석하고, 분석된 작품들의 태그를 기반으로 관련된 음악과 미술작품을 매칭한다. 또한 매칭된 작품을 활용해 융·복합 예술 관람 플레이리스트 콘텐츠를 자동으로 생성하는 기술을 제안하고자 한다.

• International Journal of Internet, Broadcasting and Communication
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• 제16권2호
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• pp.87-92
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• 2024

The rapid development of artificial intelligence technology in recent years is evident, from the emergence of ChatGPT to innovations like Midjourney, Stable Diffution, and the upcoming SORA text-to-video technology by OPENai. Animation capture technology, driven by the AI technology trend, is undergoing significant advancements, accelerating the progress of the animation industry. Through an analysis of the current application of DEEPMOTION, this paper explores the development direction of AI motion capture technology, analyzes issues such as errors in multi-person object motion capture, and examines the vast prospects. With the continuous advancement of AI technology, the ability to recognize and track complex movements and expressions faster and more accurately, reduce human errors, enhance processing speed and efficiency. This advancement lowers technological barriers and accelerates the fusion of virtual and real worlds.

• Nuclear Engineering and Technology
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• 제56권6호
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• pp.2404-2411
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• 2024

The GEOUNED code is specifically designed to convert CAD models, defined using the B-rep approach, into MC radiation transport models, defined using the CSG approach, and vice versa from MC to CAD. This code incorporates standard features commonly found in conversion tools, including decomposition, conversion, and automatic void generation. Additionally, it introduces innovative features, mainly in the automatic void generation part, which are described in this article. GEOUNED has demonstrated successful application in highly detailed 3D models used in fusion neutronics, which are known for their complex geometries, particularly those utilized in ITER. The article includes examples showcasing GEOUNED's performance in these challenging models, as well as custom applications that highlight its flexibility in addressing non-standard problems. The code is open-source and utilizes Open CASCADE as the geometry engine, with FreeCAD serving as the Python API.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.631-638
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• 2008

Tandem affinity purification (TAP) method combined with LC-MS/MS is the most accurate and reliable way to study the interaction of proteins or proteomics in a genome-wide scale. For the first time, we used a TAP-tag as a mutagenic tool to disrupt protein interactions at the specific site. Although lots of commonly used mutational tools exist to study functions of a gene, such as deletional mutations and site-directed mutagenesis, each method has its own demerit. To test the usefulness of a TAP-tag as a mutagenic tool, we applied a TAP-tag to RNA polymerase II, which is the key enzyme of gene expression and is controlled by hundreds of transcription factors even to transcribe a gene. Our experiment is based on the hypothesis that there will be interrupted interactions between Pol II and transcription factors owing to the TAP-tag attached at the C-terminus of each subunit of Pol II, and the abnormality caused by interrupted protein interactions can be observed by measuring a cell-cycle of each yeast strain. From ten different TAP-tagged strains, Rpb7- and Rpb12-TAP-tagged strains show severe defects in growth rate and morphology. Without a heterodimer of Rpb4/Rpb7, only the ten subunits Pol II can conduct transcription normally, and there is no previously known function of Rpb7. The observed defect of the Rpb7-TAP-tagged strain shows that Rpb7 forms a complex with other proteins or compounds and the interruption of the interaction can interfere with the normal cell cycle and morphology of the cell and nucleus. This is a novel attempt to use a TAP-tag as a proteomic tool to study protein interactions.

• 생명과학회지
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• 제19권5호
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• pp.561-565
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• 2009

인간조직인자는 혈액응고인자 factor VII 과 복합체를 형성하며 연속적인 혈액응고 연쇄반응을 촉매하는 효소 활성체이다. 복합체 형성에 필수적인 이 조직인자의 세포 외 부분이, 기존의 융합 단백질 및 히스티딘 말단이 없는 새로운 발현 벡터에 의해 대장균 내에서 과량 발현 되었다. 봉입체 형태로 발현된 재조합 인간조직인자는 DEAE-Sephacel 크로마토그라피 기술을 적용하여 분리, 정제 및 구조적 복원이 동시에 시도 되었다. 정제된 재조합 단백질은 SDS-PAGE 분석에서 순수한 형태로 나타났으며, 생물학적 활성도 또한 기존의 조직인자와 거의 동등함을 보였다. 본 연구의 발현 및 정제 시스템은 이전의 보고에서 보여진 방법들에 비해 단백질 분해효소를 사용하지 않아 추가적인 크로마토그라피 과정이 필요 없어 좀 더 효율적이기 때문에 기존의 발현 시스템에 대해 대체할 수 있는 매우 유용한 방법으로 제공된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7343-7350
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• 2015

Genetic changes associated with acute lymphoblastic leukemia (ALL) provide very important diagnostic and prognostic information with a direct impact on patient management. Detection of chromosome abnormalities by conventional cytogenetics combined with fluorescence in situ hybridization (FISH) play a very significant role in assessing risk stratification. Identification of specific chromosome abnormalities has led to the recognition of genetic subgroups based on reciprocal translocations, deletions and modal number in B or T-cell ALL. In the last twelve years 102 newly diagnosed childhood/adult ALL bone marrow samples were analysed for chromosomal abnormalities with conventional G-banding, and FISH (selected cases) using specific probes in our hospital. G-banded karyotype analysis found clonal numerical and/or structural chromosomal aberrations in 74.2% of cases. Patients with pseudodiploidy represented the most frequent group (38.7%) followed by high hyperdiploidy group (12.9%), low hyperdiploidy group (9.7%), hypodiploidy (<46) group (9.7%) and high hypertriploidy group (3.2%). The highest observed numerical chromosomal alteration was high hyperdiploidy (12.9%) with abnormal karyotypes while abnormal 12p (7.5%) was the highest observed structural abnormality followed by t(12;21)(p13.3;q22) resulting in ETV6/RUNX1 fusion (5.4%) and t(9;22)(q34.1;q11.2) resulting in BCR/ABL1 fusion (4.3%). Interestingly, we identified 16 cases with rare and complex structural aberrations. Application of the FISH technique produced major improvements in the sensitivity and accuracy of cytogenetic analysis with ALL patients. In conclusion it confirmed heterogeneity of ALL by identifying various recurrent chromosomal aberrations along with non-specific rearrangements and their association with specific immunophenotypes. This study pool is representative of paediatric/adult ALL patients in Oman.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권4호
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• pp.1603-1623
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• 2020

Multiple Mobile Robots System (MMRS) has shown many attractive features in lots of real-world applications that motivate their rapid and wide diffusion. In MMRS, the Cooperative Localization (CL) is the basis and premise of its high-performance task. However, the statistical characteristics of the system noise should be already known in traditional CL algorithms, which is difficult to satisfy in actual MMRS because of the numerous of disturbances form the complex external environment. So the CL accuracy will be reduced. To solve this problem, an improved Adaptive Active Cooperative Localization (A²CL) algorithm based on information optimization strategy for MMRS is proposed in this manuscript. In this manuscript, an adaptive information fusion algorithm based on the variance component estimation under Extended Kalman filter (VCEKF) method for MMRS is introduced firstly to enhance the robustness and accuracy of information fusion by estimating the covariance matrix of the system noise or observation noise in real time. Besides, to decrease the effect of observation uncertainty on CL accuracy further, an observation optimization strategy based on information theory, the Model Predictive Control (MPC) strategy, is used here to maximize the information amount from observations. And semi-physical simulation experiments were carried out to verity the A²CL algorithm's performance finally. Results proved that the presented A²CL algorithm based on information optimization strategy for MMRS cannot only enhance the CL accuracy effectively but also have good robustness.

• 한국정보통신학회논문지
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• 제15권6호
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• pp.1391-1396
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• 2011

노동 집약적인 농업의 생산성을 높일 수 있는 방법은 IT 기술을 접목시키는 것이다. 유비쿼터스 컴퓨팅의 대표적인 기술은 교통, 자동차, 건설, 군방, 헬스, 농업, 의료 서비스 등 사회 전 분야에 적용되고 있다. 이러한 IT 기술은 전통산업 간의 융합기술 개발이 새로운 화두로 부상하고 있다. 유비쿼터스 농업 환경을 성공적으로 구축하기 위해서는 센서노드 H/W, 센서노드 미들웨이 플랫폼, 라우팅 프로토콜, 농업환경 응용 서비스 등 농업에 최적화된 핵심기술 개발이 필요하다. 본 논문에서는 정밀한 식물 생장환경관리를 위해서 재배온실에 환경요인 모니터링 센서 및 생체 정보 센서들을 이용하여 그린 생장환경 관리 시스템을 제안했다. 제안한 시스템을 이용하면 사용자는 저비용으로 특화된 융복합 농업을 실현할 수 있을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제16권1호
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• pp.84-91
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• 2006

A Saccharomyces cerevisiae pgs1 nulI mutant, which is deficient with phosphatidyl glycerol (PG) and cardiolipin (CL) biosynthesis, grows well on most fermentable carbon sources, but fails to grow on non-fermentable carbon sources such as glycerol, ethanol, and lactate. This mutant also cannot grow on galactose medium as the sole carbon source. We found that the incorporation of $[^{14}C]-galactose$, which is the first step of the galactose metabolic pathway (Leloir pathway), into the pgs 1 null mutant cell was extremely repressed. Exogenously expressed PGS1 (YCpPGS1) under indigenous promoter could completely restore the pgs1 growth defect on non-fermentable carbon sources, and dramatically recovered $[^{14}C]-galactose$ incorporation into the pgs1 mutant cell. However, PGS1 expression under the GALl promoter $(YEpP_{GAL1}-PGS1myc)$ could not complement pgs1 mutation, and the GAL2-lacZ fusion gene $(YEpP_{GAL2}-lacZ)$ also did not exhibit its $\beta-galactosidase$ activity in the pgs1 mutant. In wild-type yeast, antimycin $A(1\;{\mu}g/ml)$, which inhibits mitochondrial complex III, severely repressed not only the expression of the GAL2-lacZ fusion gene, but also uptake of $[^{14}C]-galactose$. However, exogenously expressed PGS1 partially relieved these inhibitory effects of antimycin A in both the pgs1 mutant and wild-type yeast, although it could not basically restore the growth defect on galactose by antimycin A. These results suggest that the PGSI gene product has an important role in utilization of galactose by Gal genes, and that intact mitochondrial function with PGS1 should be required for galactose incorporation into the Leloir pathway. The PGS1 gene might provide a clue to resolve the historic issue about the incapability of galactose with deteriorated mitochondrial function.