• Title/Summary/Keyword: DON

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Analysis and Survey for Contamination of Deoxynivalenol and Zearalenone in Feed by High Performance Liquid Chromatography (HPLC를 이용한 사료 중 Deoxynivalenol, Zearalenone의 분석과 오염도 조사)

  • Kim, Dong-Ho;Choi, Kyu-Il;Hong, Kyung-Suk;Kim, Hyun-Jung;Song, Yeong-Jin;Gang, Seung-Hun;Jang, Han-Sub;Cho, Hyun-Jung;Han, Gye-Su
    • Journal of Food Hygiene and Safety
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    • v.26 no.3
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    • pp.214-221
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    • 2011
  • Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high performance liquid chromatography/fluorescence with diode array detector (HPLC/FLD with DAD). The average recoveries of DON were 88.76 and 95.40% at the levels of 200 and 1,000 ${\mu}g/kg$ and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 ${\mu}g/kg$, respectively. The limit of detection (LOD) were 6.0 and 3.0 ${\mu}g/kg$ for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 ${\mu}g/kg$ in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 ${\mu}g/kg$ for them, individually.

Effects of Dissolved Organic Nitrogen on the Growth of Dominant Phytoplankton in the Southwestern Part of East Sea in Late Summer (늦여름 동해 남서해역에서 용존 유기 질소가 우점 식물플랑크톤의 생장에 미치는 영향)

  • Kwon, Hyeong-Kyu;Jeon, Seul-Gi;Oh, Seok-Jin
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.1
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    • pp.42-51
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    • 2016
  • We investigated the distribution of dissolved nutrients, phytoplankton community structure and utilization of nitrogen compounds by dominant species in the southwestern part of East Sea in September, 2014. Dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus (DIP) were lower in the surface layer, and concentrations were increased with depth. Dissolved organic nitrogen (DON) and dissolved organic phosphorus were the opposite of dissolved inorganic nutrients. Although DIN DIP ratio in all of the water masses was higher than Redfield ratio (16), DIN : DIP ratio in mixed layer was about 2, indicating that inorganic nitrogen is the limiting factor for the growth of phytoplankton. In particular, DON proportion in dissolved total nitrogen was about 88 % in the mixed layer where inorganic nitrogen is limiting factor. The dominant species Chaeotceros debilis and Prorocentrum minimum were able to grow using DIN as well as DON such as urea and amino acids. Therefore, DON utilization of phytoplankton may play a role as a survival strategy in the DIN-limited conditions of East Sea.

Simultaneous Analysis and Survey for Contamination of Nivalenol, Deoxynivalenol, T-2 toxin and Zearalenone in Feed (사료 중 Nivalenol, Deoxynivalenol, T-2 foxin과 Zearalenone의 동시분석과 오염도조사)

  • Kim, Dong-Ho;Kim, Hyun-Jung;Jang, Han-Sub;Kim, Yeong-Min;Choi, Heng-Bo;Ahn, Jong-Sung
    • Journal of Food Hygiene and Safety
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    • v.26 no.1
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    • pp.1-11
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    • 2011
  • Nivalenol (NIV), deoxynivalenol (DON), T-2 toxin (T-2) and zearalenone (ZEN) are mycotoxins produced by some Fusarium species known to be very frequently contaminated in feed. The study for simultaneous analysis and contamination survey in animal feed carried out. All mycotoxins were analysed by using high performance liquid chromatography tandem mass with internal standard. The limits of detection (LOD) were $2.0\;{\mu}g/kg$, $1.0\;{\mu}g/kg$, $1.0\;{\mu}g/kg$ and $0.1\;{\mu}g/kg$ for NIV, DON, T-2 and ZEN, respectively. Two hundred and thirty nine samples of feed were collected. The average concentration of DON was $212.3\;{\mu}g/kg$, $207.8\;{\mu}g/kg$ and $812.1\;{\mu}g/kg$ in chicken, pig and cattle feed, respectively. The average concentration of ZEN was $31.2\;{\mu}g/kg$, $35.6\;{\mu}g/kg$ and $147.2\;{\mu}g/kg$ for them, respectively. Especially, the levels of contamination for DON and ZEN were higher than those of NIV or T-2. And, the levels of contamination for four Fusarium mycotoxins in cattle feed appeared higher than those of pig and chicken feed. It was investigated that the high level of mycotoxin contamination in cattle feed was caused by com gluten feed of ingredients for feed, mainly.

Correlation Between Knowledge and Educational Needs Related to Recurrent in Coronary Artery bypass graft patients (관상동맥 우회술환자의 수술 후 재발 관련 지식과 교육요구도와의 상관관계)

  • 김희승;박민정
    • Journal of Korean Academy of Nursing
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    • v.30 no.3
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    • pp.549-559
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    • 2000
  • The purpose of this study was to investigate the correlation between the knowledge and educational needs related to recurrent in coronary artery bypass graft patients as a basis to provide an individual nursing education for the population. The subjects consisted of 110 patients who had coronary artery bypass graft(CABG) at Asan Medical Center in Seoul and Sechong hospital in Buchon. Data was obtained from a knowledge questionnaire and a learning needs questionnaire between November 1998 and February 1999. Data were analyzed using SAS program for Wilcoxon rank sum test and Spearman correlation coefficient. The results were as follows : 1. With regard to the 18 items to measure knowledge, the mean (median) of items 'don't know' was 4.9(4) items. The mean (median) of items answered wrong was 3.2(3) items. The number of items answered 'don't know' tend to show higher in those who had less education, blue color jobs and myocardiac infarction history than in their counter parts. There were higher frequency of items answered 'don't know' in those who had no hypertension 2. With regard to the level of knowledge by questionnaire about CABG, The most "I dont know" (59.1%) highly response was 'He has to be treated with anticoagulant drug to prevent revasculized vessel from obstructing.' The seond highest response (56.4%) was 'If you were hypotensive, the coronary attack would collapse. 'During the hospitalized day, the patient has complete bedrest.' The highest error probability was cholesterol has not to intake.', 'After surgery, the sexual life is need controlled for 1 year. 3. The mean of educational needs was 3.38. With regard to the level of learning needs by sentence about CABG, 'Food that benefit heart disease', 'Recurrence possibility of heart disease', 'Management method of operation site', 'Risk symptom that visit hospital or report immediately' were higher than other sentenses. With regard to the level of learning needs by factor 'food(5 items)', 'disease(9 items)' and 'exercise(3 items)' showed the highest than other factors. The educational needs by patients characteristics tend to show higher in males, under the age of 49, middle or high school degree, previous experience of admission with coronary artery disease, history of myocardial infarction, expierience of PTCA, history of cerebro-vascular accident, previous expierience of smoking than in their counter parts. 4. The number of items answered 'don't know', wrong and correct weren't correlated with the level educational needs. As the results, the number of items answered 'don't know' tend to show higher in those who had less educated, blue color jobs and myocardiac infarction history than in their counter parts. There were higher frequency of items answered 'don't know' in those who had no hypertension .There were higher frequency of items answered 'don't know' on anti-thrombolitic theraphy, hypotension and pain relief. Also there were higher frequency of items answered wrong on bed rest period, cholesterol intake, and sexual life. Educational needs were higher in young age group, had previous experience of procesure and history of other disease. And when we educate CABG patients, education for diet, recurrence possibility of disease, management methods of operation site and risk symptom should be emphasized.

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The relationship between oral health behavior and dental services utilization in Korean adolescents (한국 청소년의 구강보건행태와 치과의료이용과의 연관성)

  • Choi, Moon-Sil;Park, Hyung-Su;Lee, Byung-Hoon;Jeong, Sang-Gil;Park, Jong
    • Journal of Korean society of Dental Hygiene
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    • v.10 no.5
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    • pp.851-860
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    • 2010
  • Objectives : This study examined the oral health behavior and dental services utilization of adolescents in order to provide information on how to disseminate the correct oral health behavior to the youth and develop programs for the approach. Methods : The raw data of 'The Third 2007 Youth Health Behavior Online Survey' carried out by the Korea Center for Disease Control and Prevention were analyzed. All survey data collected was processed using SPSS ver 17.0 for data analysis as Frequency analysis, chi-square and Logistic regression analysis. Results : 1.The adolescents who have parents with college degree are 70% higher of dental services utilization than the adolescents counterpart(whose parents don't have degrees). And also the adolescents living in cities are 1.3 times higher use of dental services than others living in countries. 2. In case of drinking soda, students having soda 4 days per week on average are 1.2 times higher use of dental services than who don't. 3.The students brushing teeth after lunch are 0.9 times lower use of dental service than who don't. And the students who have tooth ache whenever they have a meal are 1.6 times higher use of dental service than the students who don't have tooth ache. The students who had a dental health education are 1.4 times higher utilization of dental service than who don't. The difference of using dental service between the students who have gum disease and halitosis and the students who don't is almost none. 4. The students who drink alcohol and smoke 10 days more or less a month are 0.8 ~ 0.9 times lower use of dental service than who don't. Conclusions : The rate of utilizing dental service is higher at students who live with parents having college degrees and the more have oral health behavior, the less use of dental service for treatment. We concluded that more educational program should be developed and vitalized so that students can have oral health.

Antioxidant and Anticancer Activity of Fractions from Picrasma quassioides (D. Don) Benn. Methanolic Extract

  • Yin, Yu;Wang, Myeong-Hyeon
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.5
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    • pp.329-334
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    • 2007
  • The potential antioxidant and anticancer activities of Hexane, EtOAc (Ethyl acetate), BuOH (n-Buthanol) and water fractions from methanolic (MeOH) extract of Picrasma quassioides (D. Don) Benn. were evaluated in vitro. Tested fractions showed strong antioxidant activity, especially EtOAc fraction had the highest activity ($IC_{50}\;=\;114.01\;{\mu}g/mL$), containing high total phenolics and total flavonoids contents, showed $67.59\;Tan\;{\mu}g/mg$ and $64.95\;Que\;{\mu}g/mg$ respectively. Anticancer activity of these fractions was tested by MTT assay on HT-29 (the human colon carcinoma cells) cell line. BuOH fraction not only showed very high anticancer activity, but also had no cytotoxic effect on 293 (the human normal kidney cells) cell line. Considering these results, we used BuOH fraction of MeOH crude extract from P. quassioides (D.Don) Benn. to do assessment of apoptosis by flow cytometry and the mRNA expression levels of widely established apoptotic-related genes on HT-29 cell line. All the experiments showed that BuOH fraction can induce apoptosis on HT-29 cell line strongly. Taken together, methanolic extract of P. quassioides has potential for antioxidant and anticancer activities products.

A New Approach to the Synthesis of Two-Dimensional Cellular Arrays Using Internal Don't Cares (내부 Dont't care를 이용한 이차원 셀 배열의 새로운 합성 방법)

  • Lee, Dong-Geon;Jeong, Mi-Gyeong;Lee, Gwi-Sang
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.49 no.2
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    • pp.81-87
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    • 2000
  • This paper presents a new approach to the synthesis of two-dimensional arrays such as Atmel 6000 series FPGAs using internal don't cares. Basically complex terms which fits to the linear array of cells without further routing wires are generated and they are collected by OR/XOR operations. In previous methods, complex terms are collected only by XOR operations, which may not be effective for nearly unate functions. In this paper, we allow complex terms to be collected by OR operations in addition to XOR operations. First, complex terms that lies in the ON-set of the function are generated and collected by OR operations. The sub-function realized by the first stage becomes an internal don't cares and they are exploited in the second stage which generates complex terms collectable by XOR operation. Experimental results shows the efficacy of the proposed method compared to the previous methods.

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The Oriental Idea and the Existential Affliction of Don Quijote (돈키호테의 실존적 고뇌와 동양사상)

  • Lim, Juin
    • Cross-Cultural Studies
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    • v.22
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    • pp.151-175
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    • 2011
  • In this article, I have analyzed the signification of the death of Don Quijote from the oriental religious and philosophical point of view, comparing with the novels of Kim, DongRi. We are able to read Cervantes' hope for his absolute ideal to be lasting forever via the death of Don Quijote, in the base of a strong will named of Gukyeong's life. In the same time, we can also discovery death as lasting of life in the basis of buddhist idea and death as the unification with nature via the meeting between the living and the dead in the Montesinos Cave. Montesinos Cave symbolizes a unclassified and chaotic space, which the Shamanist can meet the dead with a extatic ceremony and both supernatural life and daily life coexist. Therefore, the symbolic death of Don Quijote in Montesinos Cave is abe to be explained by approaching to absolute freedom named of Hatal, in other words, voluntary and conscious death to escape from the yugo of being. Considering the fact that Kim, Dongri emphasized on the unification of sky, earth and human being, keeping a racial identification in the face of the stream of occidental materialism in the time of Japanese conquest, we can look for the similarity with Cervantes' ideal. Contrary to the religious dogmatic ideal, Cervantes treated to recover the human nature and vitality, transcending the occidental rationalism and religious ideology in the time of Counter-reformation.

Dominance and Survival Strategy of Toxic Dinoflagellate Alexandrium tamarense and Alexandium catenella Under Dissolved Inorganic Nitrogen-limited Conditions (용존태 무기질소 제한 하에서 유독와편모조류 Alexandrium tamarense와 Alexandium catenella의 생존과 우점화 전략)

  • Kwon, Hyeong Kyu;Park, Ji A;Yang, Han-Soeb;Oh, Seok Jin
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.16 no.1
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    • pp.25-35
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    • 2013
  • Dominance and survival strategy of toxic dinoflagellate Alexandrium tamarense and A. catenella under the dissolved inorganic nitrogen (DIN) limited conditions were examined in the laboratory and field observations. In Masan Bay, DIN was limiting factor for growth of phytoplankton during spring to early summer when Alexandrium spp. have been observed. They have a disadvantageous position compared with diatoms because Ks of nitrate calculated from growth kinetics experiment of A. tamarense and A. catenella was higher than diatoms. However, A. tamarense and A. catenella were able to grow using dissolved organic nitrogen (DON) compounds such as urea and amino acids as well as DIN. Therefore, DON utilization of A. tamarense, A. catenella might contribute to not only their population growth but also dominance and interspecific competition in the DIN-limited conditions in Masan Bay.

The Fundamental Study of Academic and Career Counseling Program Development for Students of Engineering College who don't have aptitude on Engineering: A Study with Using Holland Career Aptitude Test (공과대학 비적성 학생들의 진로지도 프로그램 개발을 위한 기초 연구: Holland 직업적성검사를 중심으로)

  • Park, Sun-Hee;Kim, Keon-Hyeong;Choi, Keum-Jin
    • Journal of Engineering Education Research
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    • v.14 no.2
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    • pp.21-29
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    • 2011
  • The purpose of this study is to develop the academic and career counseling program for students of engineering college who don't have aptitude in engineering. Therefore we began with analyzing the outcome of Holland career aptitude test from enrolled students of engineering college. The method of this study is first, we classified between students who have aptitude on engineering and who don't. Next, we compared grades of between them. Lastly, we studied the needs of the students who don't have aptitude in engineering. The final result and analysis of study were used for suggestion of develop academic and career counseling program for students of engineering college who don't have aptitude on engineering.