Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Antioxidant and Anticancer Activity of Fractions from Picrasma quassioides (D. Don) Benn. Methanolic Extract

  • Yin, Yu (School of Biotechnology, Kangwon National University) ;
  • Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
  • Published : 2007.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

The potential antioxidant and anticancer activities of Hexane, EtOAc (Ethyl acetate), BuOH (n-Buthanol) and water fractions from methanolic (MeOH) extract of Picrasma quassioides (D. Don) Benn. were evaluated in vitro. Tested fractions showed strong antioxidant activity, especially EtOAc fraction had the highest activity ($IC_{50}\;=\;114.01\;{\mu}g/mL$), containing high total phenolics and total flavonoids contents, showed $67.59\;Tan\;{\mu}g/mg$ and $64.95\;Que\;{\mu}g/mg$ respectively. Anticancer activity of these fractions was tested by MTT assay on HT-29 (the human colon carcinoma cells) cell line. BuOH fraction not only showed very high anticancer activity, but also had no cytotoxic effect on 293 (the human normal kidney cells) cell line. Considering these results, we used BuOH fraction of MeOH crude extract from P. quassioides (D.Don) Benn. to do assessment of apoptosis by flow cytometry and the mRNA expression levels of widely established apoptotic-related genes on HT-29 cell line. All the experiments showed that BuOH fraction can induce apoptosis on HT-29 cell line strongly. Taken together, methanolic extract of P. quassioides has potential for antioxidant and anticancer activities products.

Keywords

References

  1. Athukorala Y, Kim KN, Jeon YJ (2006) Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem Toxicol. 44:1065-­1074 https://doi.org/10.1016/j.fct.2006.01.011
  2. Birt DF, Hendrich S, Wang WQ (2001) Dietary agents in can­cer prevention: flavonoids and isoflavonoids. Pharmacol Ther. 90:157-177 https://doi.org/10.1016/S0163-7258(01)00137-1
  3. Chen YL, Lin SZ, Chang JY, Cheng YL, Tsai NM, Chen SP, Chang WL, Harn HJ (2006) In vitro and in vivo studies of a novel potential anticancer agent of isochaihulactone on human lung cancer A549 cells. Biochem Pharmacol. 72:308-­319 https://doi.org/10.1016/j.bcp.2006.04.031
  4. Ching Su New Medical College (ed) (1977) Dictionary of Chi­nese Materia Medica: hanghai Scientific Technological Pub­lishers. Shangai. 1294
  5. Chiu LCM, Ho TS, Wong EYL, Ooi VEC (2006) Ethyl acetate extract of Patrinia scabiosaefolia downregulates anti-apop­totic Bcl-2/Bcl-XL expression, and induces apoptosis in human breast carcinoma MCF-7 cells independent of caspase-9 activation. J Ethnopharmacol. 105:263-268 https://doi.org/10.1016/j.jep.2005.11.007
  6. Gordon MH (1990) The mechanism of antioxidant action in vitro. In Hudson (Ed.). Food antioxidants. Elsevier. London. 1-18
  7. Halliwell B (1995) Antioxidant characterization: methodology and mechanism. Biochem Pharmacol. 49: 1341-1348 https://doi.org/10.1016/0006-2952(95)00088-H
  8. Halliwell B, Gutteridge JMC (1985) Free radicals in biology and medicine. Oxford University Press. Oxford, 218-313
  9. Ju EM, Lee SE, Hwang HJ, Kim JH (2004) Antioxidant and anticancer activity of extract from Betula platyphylla var. japonica. Life Sci. 74:1013-1026 https://doi.org/10.1016/j.lfs.2003.07.025
  10. Kogure K, Manabe S, Hama S, Tokumura A, Fukuzawa K (2003) Potentiation of anti-cancer effect by intravenous administration of vesiculated a-tocopheryl hemisuccinate on mouse melanoma in vivo. Cancer Lett. 192:19-24 https://doi.org/10.1016/S0304-3835(02)00683-3
  11. Kousteni S, Tura-Kockar F, Ramji DP (1999) Sequence and expression analysis of a novel Xenpus laevis cDNA that encodes a protein similar to bacterial and chloroplast riboso­mal protein L24. Gene. 235:13-18 https://doi.org/10.1016/S0378-1119(99)00221-8
  12. Lavia I, Friesemb D, Gereshc S, Hadarb Y, Schwartza B (2006) An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells, Cancer Lett. 244:61-70 https://doi.org/10.1016/j.canlet.2005.12.007
  13. Le Baila JC, Varnata F, Nicolasb JC, Habriouxa G (1998) Estrogenic and antiproliferative activities on MCF-7 human breast cancer cells by flavonoids. Cancer Lett. 130:209-216 https://doi.org/10.1016/S0304-3835(98)00141-4
  14. Lee CL, Yang XT, Wan JMF (2006) The culture duration affects the immunomodu1atory and anticancer effect of polysaccharopeptide derived from Corio Ius versicolor. Enzyme Microb Technol. 38:14-21 https://doi.org/10.1016/j.enzmictec.2004.10.009
  15. Lin JY, Tang CY (2007) Determination oftotal phenolic and fla­vonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem. 101 :140-147 https://doi.org/10.1016/j.foodchem.2006.01.014
  16. Lu YR, Foo LY (1997) Identification and quantification of major polyphenols in apple pomace. Food Chem. 59:187­-194 https://doi.org/10.1016/S0308-8146(96)00287-7
  17. Mossman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  18. Pakunlu RI, Wang Y, Saad M, Khandare JJ, Starovoytov V, Minko T (2006) In vitro and in vivo intracellular liposoma1 delivery of antisense oligonucleotides and anticancer drug. J Control Release. 114:153-162 https://doi.org/10.1016/j.jconrel.2006.06.010
  19. Popiolkiewicz J, Polkowski K, Skierski JS, Mazurek AP (2005) In vitro toxicity evaluation in the development of new anticancer drugs-genistein glycosides. Cancer Lett. 229:67­75 https://doi.org/10.1016/j.canlet.2005.01.014
  20. Qia LF, Xua ZR (2006) In vivo antitumor activity of chitosan nanoparticles. Bioorg Med Chem Lett. 16:4243-4245 https://doi.org/10.1016/j.bmcl.2006.05.078
  21. Schmitt CA (2006) Cellular senescence and cancer treatment. Biochim Biophys Acta. 21: 132-136
  22. Shyu YS, Hwang LS (2002) Antioxidant activity of the crude extract of 1ignan glycosides fromunroasted Burma black ses­ame meal. Food Res. Intern. 35:357-365 https://doi.org/10.1016/S0963-9969(01)00130-2
  23. Slater TF, Sawyer B, Strauli UD (1963) Studies on succinate­tetrazolium reductase system. III. Points of coupling of four different tetrazolium salts. Biochim Biophys Acta. 77:383-393 https://doi.org/10.1016/0006-3002(63)90513-4
  24. Tan ML, Sulaiman SF, Najimuddin N, Samian MR, Tengku Muhammad TS (2005) Methanolic extract of Pereskia bleo (Kunth) DC. (Cactaceae) induces apoptosis in breast carci­noma, T47-D cell line. J Ethnopharmacol. 96:287-294 https://doi.org/10.1016/j.jep.2004.09.025
  25. Tengku Muhammad TS, Hughes TR, Ranki H, Cryer A, Ramji DP (2000) Differential regulation of macrophage CCAAT-Enhancer binding protein isoforms by lipopolysac­charide and cytokines. Cytokine. 12:1430-1436 https://doi.org/10.1006/cyto.2000.0711
  26. Voss C, Eyol E, Berger MR (2005) Identification of potent anti­cancer activity in Ximenia americana aqueous extracts used by African traditional medicine. Toxicol Appl Pharmacol. 12:312-321
  27. Wang XJ, Wei YQ, Yuan SL, Liu GJ, Lu YR, Zhang J, Wang WD (2006) Potential anticancer activity of litchi fruit peri­carp extract against hepatocellular carcinoma in vitro and in vivo. Cancer Lett. 239:144-150 https://doi.org/10.1016/j.canlet.2005.08.011
  28. Wang XJ, Yuan SL, Wang J, Lin P, Lin GJ, Lu YR, Zhang J, Wang WD, Wei YQ (2006) Anticancer activity oflitchi fruit pericarp extract against human breast cancer in vitro and in vivo. Toxicol Appl Pharmacol. 215:168-178 https://doi.org/10.1016/j.taap.2006.02.004
  29. Wang Y, Chan FL, Chen Sh, Leung LK (2005) The plant po1yphenol butein inhibits testosterone-induced proliferation in breast cancer cells expressing aromatase. Life Sci. 77:39-­51 https://doi.org/10.1016/j.lfs.2004.12.014
  30. Yin Y, Heo SI, Jung MJ, Wang MH (2007) Antioxidant Prop­erties of Water Extract from Acorn, J. Appl. Biol. Chem. 50:70-73
  31. Yoshikawa K, Sugawara S, Arihara S (1995) Pheny1pro­panoids and other secondary metabolites from fresh fruits of Picrasma Quassioides. Phytocheraistry. 40:253-256 https://doi.org/10.1016/0031-9422(95)00234-X
  32. Zhang T, Lu H, Shang X, Tian YH, Zheng CY, Wang SW, Cheng HHa, Zhou RJ (2006) Nicotine prevents the apopto­sis induced by menadione in human lung cancer cells. Bio­chem Biophys Res Commun. 342:928-934 https://doi.org/10.1016/j.bbrc.2006.02.047
  33. Zhao MM, Yang B, Wang JS, Li BZ, Jiang YM (2006) Identi­fication of the major flavonoids from pericarp tissues of lychee fruit in relation to their antioxidant activities. Food Chem. 98:539-544 https://doi.org/10.1016/j.foodchem.2005.06.028