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Expression of OsPTs-OX Transgenic Rice in Phosphate-Deficient Condition (무인산 조건에서 OsPTs 유전자 도입 형질전환벼의 인산흡수 반응)

  • Song, Song-Yi;Yi, Gi-Hwan;Park, Dong-Soo;Seo, Jong-Ho;Son, Beom-Young;Kim, Do-Hoon;Nam, Min-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.3
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    • pp.264-272
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    • 2011
  • It needs to develop high phosphate-available rice that is able to minimize environmental pollution caused by phosphate fertilizer. Then we have transformed 4 rice transporter genes, OsPT(Oryza sativa Phosphate Transporter)1, OsPT4, OsPT7 or OsPT8, to rice (Oryza sativa cv. Dongjin) via Agrobacterium-mediated transformation. We tested adaptation in the P-deficient condition of Dongjin (parental) and each transgenic line in the pot and the field conditions. Definite physiological changes have been observed in OsPTs transgenic lines including culm length, root formation and heading date. Phosphate uptake at harvesting stage was about three times higher in OsPT1-OX (overexpression) and OsPT4-OX than in Dongjin (wt) without P application. There are no variations in total phosphate-content of brown rice of OsPT1-OX in spite of high phosphate uptake. Practically the expression of OsPT1 has contributed to stabilize grain production without P fertilization in rice cultivation than Dongjin.

Hypoglycemic Effects of Fruits and Vegetables in Hyperglycemic Rats for Prevention of Type-2 Diabetes (고혈압쥐의 과일과 야채의 섭취에 따른 저혈당 효과)

  • Survay, Nazneen Shaik;Ko, Eun-Young;Upadhyay, Chandrama Prakash;Jang, Mi;Park, Se-Won;Lee, Dong-Ha;Jung, Yi-Sook;Yoon, Do-Young;Hong, Sae-Jin
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.850-856
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    • 2010
  • An in vivo oral glucose tolerance test (OGTT) was performed on hyperglycemic male Sprague-Dawley rats to assess the effect of fruits and vegetables ($1g{\cdot}kg^{-1}$ body weight) on blood glucose levels (${\Delta}BGLs$) at different time intervals of 0, 5, 15, 30, 60, 90 and 120 min. The areas under glucose curve (${\Delta}AUCs$) were calculated at 120 min of OGTT by trapezoid method. Total phenolic content (TPC) and anti-oxidant activity (AOA) of fruits and vegetables were assayed in vitro by Folin Ciocalteu and DPPH (2, 2-diphenyl-1-picrylhydrazyl) methods, respectively. At the end of the experiment the correlations among the parameters TPC, AOA and ${\Delta}AUC$ was estimated by Pearson's correlations. Among fruit crops, tangerine, plum, grape and pear and among vegetables, blue leaf mustard, cabbage, chicory, broccoli and others exhibited significant hypoglycemic effects by reducing ${\Delta}BGLs$ with significant ${\Delta}AUC$. The effective ${\Delta}AUC$ ranged from $5548.2{\pm}462.1$ to $3823.3{\pm}282.0mg-min{\cdot}dL^{-1}$. The TPC and AOA ranged from $0.063{\pm}0.00$ to $0.913{\pm}0.14mg{\cdot}g^{-1}$ GAE and $01.05{\pm}0.08$ to $75.46{\pm}0.06%$, respectively. Overall, six fruits and fifteen vegetables exhibited higher TPC and one fruit and four vegetables exhibited higher AOA. There was a better correlation among TPC, AOA and ${\Delta}AUC$ of fruits and TPC & AOA of vegetables. We report that hypoglycemically significant fruits and vegetables investigated in this study have pharmacological importance which reduced ${\Delta}BGLs$ through insulin like activity and AOA in prevention of type-2 diabetes.

Detecting the Climate Factors related to Dry Matter Yield of Whole Crop Maize (사일리지용 옥수수의 건물수량에 영향을 미치는 기후요인 탐색)

  • Peng, Jing-lun;Kim, Moon-ju;Kim, Young-ju;Jo, Mu-hwan;Nejad, Jalil Ghassemi;Lee, Bae-hun;Ji, Do-hyeon;Kim, Ji-yung;Oh, Seung-min;Kim, Byong-wan;Kim, Kyung-dae;So, Min-jeong;Park, Hyung-soo;Sung, Kyung-il
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.17 no.3
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    • pp.261-269
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    • 2015
  • The purpose of this research is to identify the significance of climate factors related to the significance of change of dry matter yield (DMY) of whole crop maize (WCM) by year through the exploratory data analysis. The data (124 varieties; n=993 in 7 provinces) was prepared after deletion and modification of the insufficient and repetitive data from the results (124 varieties; n=1027 in 7 provinces) of import adaptation experiment done by National Agricultural Cooperation Federation. WCM was classified into early-maturity (25 varieties, n=200), mid-maturity (40 varieties, n=409), late-maturity (27 varieties, n=234) and others (32 varieties, n=150) based on relative maturity and days to silking. For determining climate factors, 6 weather variables were generated using weather data. For detecting DMY and climate factors, SPSS21.0 was used for operating descriptive statistics and Shapiro-Wilk test. Mean DMY by year was classified into upper and lower groups, and a statistically significant difference in DMY was found between two groups (p<0.05). To find the reasons of significant difference between two groups, after statistics analysis of the climate variables, it was found that Seeding-Harvesting Accumulated Growing Degree Days (SHAGDD), Seeding-Harvesting Precipitation (SHP) and Seeding-Harvesting Hour of sunshine (SHH) were significantly different between two groups (p<0.05), whereas Seeding-Harvesting number of Days with Precipitation (SHDP) had no significant effects on DMY (p>0.05). These results indicate that the SHAGDD, SHP and SHH are related to DMY of WCM, but the comparison of R2 among three variables (SHAGDD, SHP and SHH) couldn't be obtained which is needed to be done by regression analysis as well as the prediction model of DMY in the future study.

Comparison of Perception on the Korean Gifted Education in Science among the Science Teachers of Formal Schools for the Gifted in Science, District Institutes for the Gifted Education, and General Secondary Schools (정규 과학영재학교, 교육청 영재교육원, 일반 중.고등학교 과학교사의 과학영재교육에 대한 인식 비교 연구)

  • Hwang, Jung-Hoon;Kim, Young-Min
    • Journal of Gifted/Talented Education
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    • v.19 no.3
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    • pp.697-727
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    • 2009
  • The purposes of this study are to investigate Korean science teachers' perception on the science gifted education in Korea, and to investigate if there are differences on their perception among the science teachers whose institutes are different. Their institutes are divided into three groups, which are the formal schools for the gifted in science such as Science High-School, gifted education centers attached to district education authority, and general secondary schools. For the study, 266 science teachers were sampled from the schools in Busan Metro-city, Ulsan Metro-city, and Kyoungnam Province, and then questionnaire developed by the authors were administered to them. The research results are as follows. Firstly, teachers who were going to participate in special education for the gifted in science answered affirmatively, and they thought it seemed worthwhile. On the other hand teachers who were not going to participate in it, answered negatively, mentioning shortage in specialty or extra work overload. Secondly, only the Korean Educational Development Institute was too much preferred by science teachers for teacher training institute for the gifted education in science. Therefore, it is needed to extend the teacher training institutes for the gifted education in science throughout the country. Thirdly, the perception of science teachers on the constitution of a class for the gifted in science is very different among the teachers of formal schools for the gifted in science and the teachers who teach science part time when it is needed in the district institutes for the gifted education. Finally, the perception on the aims of special education for the gifted in science is very different between the teachers who teach the gifted in science and the teachers who do not teach them.

Growth Patterns of Lactic Acid Bacteria during Fermentation of Radish with Rice Water and Rice Bran (쌀뜨물과 쌀겨를 이용한 무 발효과정 중 젖산균의 증식 양상)

  • Cho, Joon-Il;Jung, Hye-Jin;Ha, Sang-Do;Kim, Keun-Sung
    • Korean Journal of Food Science and Technology
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    • v.36 no.5
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    • pp.837-841
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    • 2004
  • Changes in total aerobic bacteria (TAB), lactic acid bacteria (LAB), rod- and coccal-shaped lactic acid bacteria (R- and C-LAB), pH, and total acidity were investigated during fermentation of radish with new fermentation base at $20^{\circ}C$ for up to 16 days. New fermentation base was prepared by pre-fermenting a mixture of rice bran and rice water (1 : 0.1, w/v) at $20^{\circ}C$ for 7 days. Initially, radish showed 5.41, 4.23, 4.57, and 3.1 log CFU/g, and base showed 6.68, 6.60, 5.95, and 5.6 log CFU/mL for TAB, LAB, R-LAB, and C-LAB, respectively. Initial pH and total acidity of radish were $6.6^{\circ}C$ and 0.09%, and those of base were 5.76 and 0.36%, respectively, Counts of LAB (4.23 to 8.33 Bog Cpu/g, 6.6 to 9.7 log CFU/mL), R-LAB (4.57 to 7.15 log CFU/g, 5.95 to 8.5 log CFU/mL), and C-LAB (3.1 to 7.5 log CFU/g, 5.6 to 8.6 log CFU/mL) of radish and base respectively increased during initial fermentation period, then remained constant during late fermentation period, and far 4 days after fermentation, pH values (6.6 to 4.19, 5.76 to 4.57) drastically decreased and thereafter slowly decreased. Total acidities of radish and base (0.09 to 0.63%, 0.36 to 0.63%, respectively) drastically increased far 7 days after fermentation and increased slightly thereafter.

Estimating Precise Spatio-Temporal Distribution of Weather Condition Using Semi-Variogram in Small Scale Recreation Forest (Semi-Variogram을 이용한 소규모 자연휴양림 내기상조건의 정밀 시공간 분포 추정)

  • LIM, Chul-Hee;RYU, Dong-Hoon;SONG, Chol-Ho;ZHU, Yong-Yan;LEE, Woo-Kyun;KIM, Min-Seon
    • Journal of the Korean Association of Geographic Information Studies
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    • v.18 no.3
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    • pp.63-75
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    • 2015
  • As forest therapy is getting more attention than ever, it is important to organize time for activity and location based on spatio-temporal distribution of weather condition in forest. This study aimed to analyze precise spatio-temporal distribution of weather condition by installing long-term weather monitoring device in Yonghyun national natural recreation forest and using acquired weather data in order to support forest recreation and therapy activity. First, we statistically compared 4 models of semi-variogram and the results were all similar. We selected and analyzed the circular model for this study because it was presumed to be the best model for this case. We derived 128 results from the circular model and through semi-variogram, we identified seasonal and temporal distributions of temperature and humidity. Then, we used boxplot, made of partial sill level, to identify significant differences in seasonal and temporal distributions. As a result, in spring and early morning, both temperature and humidity showed equalized result. On the other hand, in summer and early afternoon, both temperature and humidity showed uneven result. In spring and early morning, changes in weather condition are shown little from spatial shifting, it is ideal to perform recreational activities and forest therapy but in summer and early afternoon, it is unadvisable to do so as the changes in weather condition could be harmful unless any other means of preparations are made. This study proposes its significance by analyzing seasonal micro-weather of single recreation forest and presenting seasonal and temporal outcomes.

Uncertainty analysis of quantitative rainfall estimation process based on hydrological and meteorological radars (수문·기상레이더기반 정량적 강우량 추정과정에서의 불확실성 분석)

  • Lee, Jae-Kyoung
    • Journal of Korea Water Resources Association
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    • v.51 no.5
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    • pp.439-449
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    • 2018
  • Many potential sources of bias are used in several steps of the radar-rainfall estimation process because the hydrological and meteorological radars measure the rainfall amount indirectly. Previous studies on radar-rainfall uncertainties were performed to reduce the uncertainty of each step by using bias correction methods in the quantitative radar-rainfall estimation process. However, these studies do not provide comprehensive uncertainty for the entire process and the relative ratios of uncertainty between each step. Consequently, in this study, a suitable approach is proposed that can quantify the uncertainties at each step of the quantitative radar-rainfall estimation process and show the uncertainty propagation through the entire process. First, it is proposed that, in the suitable approach, the new concept can present the initial and final uncertainties, variation of the uncertainty as well as the relative ratio of uncertainty at each step. Second, the Maximum Entropy Method (MEM) and Uncertainty Delta Method (UDM) were applied to quantify the uncertainty and analyze the uncertainty propagation for the entire process. Third, for the uncertainty quantification of radar-rainfall estimation at each step, two quality control algorithms, two radar-rainfall estimation relations, and two bias correction methods as post-processing through the radar-rainfall estimation process in 18 rainfall cases in 2012. For the proposed approach, in the MEM results, the final uncertainty (from post-processing bias correction method step: ME = 3.81) was smaller than the initial uncertainty (from quality control step: ME = 4.28) and, in the UDM results, the initial uncertainty (UDM = 5.33) was greater than the final uncertainty (UDM = 4.75). However uncertainty of the radar-rainfall estimation step was greater because of the use of an unsuitable relation. Furthermore, it was also determined in this study that selecting the appropriate method for each stage would gradually reduce the uncertainty at each step. Therefore, the results indicate that this new approach can significantly quantify uncertainty in the radar-rainfall estimation process and contribute to more accurate estimates of radar rainfall.

Classification of Quercus mongolica Stand Types at Mt. Joongwang, Kangwon-Do and Determination of Proper Future Tree Density for Forest Tending Work (중왕산(中旺山) 지역(地域) 신갈나무림(林)의 임분형(林分形) 구분(區分) 및 육림작업(育林作業)에 적절(適切)한 미래목(未來木) 본수(本數)의 결정(決定))

  • Choi, Seon Deok;Lee, Don Koo;Ma, Sang Kyu
    • Journal of Korean Society of Forest Science
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    • v.87 no.4
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    • pp.631-641
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    • 1998
  • The objectives of this study were 1) to classify the types of Quercus mongolica stands at Mt. Joongwang and compare their quality, and 2) to determine the proper future tree number of Q. mongolica per ha and the appropriate distance between the future trees. The results from this study were as follows : Q. mongolica stands at Mt. Joongwang was classified into four types, pure Q. mongolica stand as stand type I, Q. mongolica - hardwood stand as stand type II, Q. mongolica - Pines densiflora stand as stand type III, Hardwood - Q. mongolica stand as stand type IV, according to mixture rate in stand volume. Stand type IV showed the best quality stem of Q. mongolica among the stand types, and the stem quality of Q. mongolica in Q. mongolica stand mixed with hardwood as stand types II and IV was better than those in pure Q. mongolica stand as stand type I and in Q. mongolica - P. densiflora stand as stand type III. If the management goal for Q. mongolica stand is to produce its high quality-timber, it is desirable to sustain proper mixture rate of Q. mongolica with another hardwoods. The proper number of future trees in pure Q. mongolica stand as stand type I was 122trees/ha and reasonable distance between the future trees was 9.15m. The distance between future trees in other stand types was 7.2m to 9.3m for stand types II and IV, while 8.0m for stand type III. Thus, the classification of Q. mongolica stand type based on stand character and maturity, and proper stem number of future tree and optimum distance between future trees would be a useful forest tending work.

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An Analysis of the Difference in Awareness on Visual Landscape Control Elements among the Expert Groups (경관제어요소에 관한 전문가집단 간 인식차이 분석)

  • Cho, You-Kyung;Kong, Eun-Mi;Kim, Young-Ook
    • Journal of the Korean Institute of Landscape Architecture
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    • v.39 no.2
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    • pp.29-39
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    • 2011
  • Recent interests in the quality of urban space have raised awareness on the role and importance of landscape planning. Although laws and guidelines are officially ready to be imposed as for landscape planning, we do not have concrete materials that can be utilized in the course of practices. The aim of this paper in this regard is to disclose the possible difference in awareness on 'visual landscape control elements' among experts engaged with urban space planning. The expert groups are distinguished to three which are for a planning, design and engineering and the survey is made by questionnaires. The results are analyzed through basic technology statistics in SPSS and independent-sample t-test provided. The survey is done by tens of 'control elements' and the result is that specially, group 1 and group 2 in mixed landscape has the most discrepancy in awareness on those elements but relatively, they has less discrepancy in awareness on compare with other groups through all landscape area. In case of artificial landscape and mixed landscape in 'landscape controled area', the result for comparing between G1 and G2 is that there are the most discrepancy in awareness which are 7 control elements. In case of mixed landscape in 'landscape promoted area', there are 4 control elements for discrepancy in awareness between G2 and G3 which is quite different. The control elements which show the most discrepancy in awareness is height, floor space and building to land ratio in order. The shape elements has only discrepancy in awareness for comparing between G1 and G2 of artificial landscape in 'landscape controled area'. In terms of the average evaluation score of the appropriateness of these control elements, G1 seems to appreciate the role of these elements in systematic landscape planning more than the other group does. In other words, relatively low scores are given by G2 as for the overall functionality of visual landscape control elements. The texture, floor space and building of land ratio has low evaluation score for all area and types. It means that it should reverify for appropriateness of performance for landscape planning as visual landscape control elements.

Influence of Preferred Chewing Habit on Electromyographic Activity of Masticatory Muscles and Bite Force (편측저작이 저작근의 근활성도와 교합력에 미치는 영향)

  • Yang, Ho-Yeon;Shin, Jun-Han;Choi, Jong-Hoon;Ahn, Hyoung-Joon
    • Journal of Oral Medicine and Pain
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    • v.30 no.1
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    • pp.45-55
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    • 2005
  • As people prefer to use right or left hand, some have preferred chewing side while others do not. Totally, 82 volunteers composed of students and staffs from Dental Hospital College of Dentistry Yonsei University participated in this study for the investigation of influence of preferred chewing habit, that has lasted for more than a year, on electromyographic(EMG) activity of masticatory muscles and bite force. Among the 82 volunteers, 46 had preferred chewing habit while the other 36 did not. Prior to the investigation, those with factors that could affect the study, such as, general disease, irregular dentition and malocclusion, were screened and excluded by questionnaire and clinical examination. The results were as follows: 1. There was no significant difference in EMG activities between chewing side and non-chewing side of preferred chewing subjects at rest as well as maximal voluntary contraction(MCV)(p>0.05). 2. Asymmetrical coefficient of temporal and masseter muscle EMG activities between preferred chewing subjects and non-preferred chewing subjects at rest was not significantly different(p>0.05). 3. Asymmetrical coefficient of masseter EMG activity was significantly higher(p<0.05) than that of non-preferred chewing subjects at MCV, whereas that of anterior temporal muscle showed no difference(p<0.05). 4. In preferred chewing subjects, there was no significant difference in average bite force and occlusal contact area between chewing side and non-chewing side(p>0.05). 5. There was no significant difference in Asymmetrical coefficients of average bite force and occlusal contact area between preferred chewing subjects and non-preferred chewing subjects (p>0.05). Consequently, preferred chewing habit can be considered as physiological asymmetry with normal function rather than to have influence on EMG muscle activity of masticatory muscles, average bite force and occlusal contact area. Objective standardization to differentiate preferred chewing subjects and non-preferred chewing subjects should be established in the further study.