• Title/Summary/Keyword: D-BLAST

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Isolation and characterization of four unrecorded wild yeasts from the soils of Republic of Korea in winter

  • Yuna Park;Soohyun Maeng;Sathiyaraj Srinivasan
    • Journal of Species Research
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    • v.12 no.3
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    • pp.197-202
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    • 2023
  • The purpose of this study was to isolate and identify wild yeasts from the soil collected in Gwangju and Pocheon City, Gyeonggi Province, Republic of Korea. Among 10 strains, six strains were already reported, but four strains were unrecorded in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were performed using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were carried out using API 20C AUX kit. The 10 strains were assigned to the phyla Basidiomycota (8 strains) and Ascomycota (2strains). The unrecorded four yeast strains, NH33, NH19, NH20, and YP416, belong to the phylum Basidiomycota and the genera Buckleyzyma, Leucosporidium, Holtermanniales, and Mrakia, respectively. All strains had oval-shaped and polar budding cells. In this research, the morphological and biochemical properties of four unreported yeast species were characterized intensively, which were not officially reported in Korea.

The numerical analysis of gas blast arcs for 800kV GCB (800kV 가스차단기의 아크특성 해석)

  • Chulkov, V.V.;Shin, Y.J.;Park, K.Y.;Song, K.D.;Choi, Y.K.
    • Proceedings of the KIEE Conference
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    • 1995.07c
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    • pp.1287-1289
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    • 1995
  • For the analysis of hot gas flow due to arc in puffer type $SF_6$ gas circuit breakers(GCBs), a program has been developed by adding function for arcing to the Fluid-in Cells(FLIC) method, which is often used for a two dimensional compressible flow problems, utilizing a simplified enthalpy flow arc model available for arcing. In this paper, the results of arc modelling for 800kV GCB are presented and compared with that of cold gas flow in the interrupters. It is shown that the nozzle clogging is the dominating factor in the pressure rise of the puffer chamber. It permits to estimate the dielectric strength of interrupters.

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Solution Structure of pA2, the Mimotopic Peptide of Apolipoprotein A-I, by NMR Spectroscopy

  • Won, Ho-Shik
    • Bulletin of the Korean Chemical Society
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    • v.32 no.11
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    • pp.4016-4020
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    • 2011
  • A number of mimetic peptides of apolipoprotein A-I, a major component for high density lipoproteins (HDL), were screened from the phase-displayed random peptide library by utilizing monoclonal antibodies (A12). A mimetic peptide for A12 epitope against apolipoprotein A-I was selected as FVLVRDTFPSSVCCP(pA2) exhibiting 45% homology with Apo A-I in the BLAST search. Solution structure determination of this mimotope was made by using 2D-NMR data and NMR-based distance geometry (DG)/molecular dynamic calculations. The resulting DG structures had low penalty value of 0.4-0.6 ${\AA}^2$ and the total RMSD of 0.7-1.7 ${\AA}$. The mimotope pA2 exhibited a characteristic ${\beta}$-turn conformation from Val[2] to Phe[8] near Pro[9] residue.

Industrial Solids Processing Applications - Particle Reaction Models and Bed Reactor Models (산업용 고체 처리 공정 - 입자 반응 및 고정층 반응기 모델링)

  • Ahn, Hyungjun;Choi, Sangmin
    • Journal of the Korean Society of Combustion
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    • v.22 no.2
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    • pp.27-35
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    • 2017
  • This paper reviews the previous industrial solid bed process simulations to provide a better understanding of the modeling approaches to the particle reactions in the bed. Previous modeling studies on waste incinerator, iron ore sintering bed, blast furnace, iron ore pellet indurator, and biomass combustor can be seen on the common ground of unsteady 1-D modeling scheme. Approaches to the particle reaction modeling have been discussed in terms of the status of solid particles in the bed, types of reaction progression in a particle, and the consideration of the intra-particle temperature gradient.

A Numerical Simulation of Projectile Aerodynamics Using a Ballistic Range (Ballistic Range를 이용한 Projectile 공기역학의 수치모사)

  • Jung S. J.;Rajesh G.;Kim H. D.;Lee J. M.
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • v.y2005m4
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    • pp.386-393
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    • 2005
  • The objective of the present study is to develop a new type of the Ballistic range, called 'two-stage light gas gun'. A computational work has been performed to investigate the aerodynamics of a projectile which is launched from the two-stage light gas gun. A moving coordinate method for a multi-domain technique is employed to simulate unsteady projectile flows with a moving boundary. The effect of a virtual mass is added to the axisymmetric unsteady Euler equation systems. The computed results reasonably capture the major flow characteristics which are generated in launching the projectile supersonically, such as the interaction between the shock wave and the blast wave, the interaction between the vortical flow and the barrel shock, and the steady under-expanded jet. The present computational results properly predict the velocity, acceleration, and drag histories of the projectile.

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On the Study of Blasting Vibration, Sound by Measuring Gage Influence to Exist Crack in 154kV Daeshin Electric Cable Tunnel (154kV 대신 S/S인출 전력구 시험발파 패턴 및 진동, 소음 계측에 의한 기존 CRACK에 미치는 영향 연구)

  • 강대우;박태원
    • Explosives and Blasting
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    • v.16 no.3
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    • pp.25-34
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    • 1998
  • This area is covered in Andesite of high compression strength and located in PUSAN SEO-KU. There are many old houses around shaft site. So, we must have a cautious blasting operation. A total of 40 blasts were test at DAE-SHIN Shaft site to study the magnitude and frequency characterization of blast-induced vibration. The effect of viblating frequency on structual damage and site-specific scaling to define th empirical equations were also discussed. The result can be summarized as follows: 1. Some empirical equations were obtained. $V=K\{{\frac{D}{W}}1/3\}^{-n}$ where the values for n and K are estimated to be -1.407 to -2.202 and 643.3489 to 7283.2104 respectively. 2. Dominant frequencies at short distance are in the range of about 75.0 to 91.8 Hz, with some exceptions of about 50Hz, Frequencies observed at long distance are in the range of 10 to 2Hz. It is apparent the shift of dominant frequency down to lower levels at long distance.

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Analysis of RC beams subjected to shock loading using a modified fibre element formulation

  • Valipour, Hamid R.;Huynh, Luan;Foster, Stephen J.
    • Computers and Concrete
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    • v.6 no.5
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    • pp.377-390
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    • 2009
  • In this paper an improved one-dimensional frame element for modelling of reinforced concrete beams and columns subjected to impact is presented. The model is developed in the framework of a flexibility fibre element formulation that ignores the shear effect at material level. However, a simple shear cap is introduced at section level to take account of possible shear failure. The effect of strain rate at the fibre level is taken into account by using the dynamic increase factor (DIF) concept for steel and concrete. The capability of the formulation for estimating the element response history is demonstrated by some numerical examples and it is shown that the developed 1D element has the potential to be used for dynamic analysis of large framed structures subjected to impact of air blast and rigid objects.

Experimental studies on rheological properties of smart dynamic concrete

  • Bauchkara, Sunil D.;Chore, H.S.
    • Advances in concrete construction
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    • v.5 no.3
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    • pp.183-199
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    • 2017
  • This paper reports an experimental study into the rheological behaviour of Smart Dynamic Concrete (SDC). The investigation is aimed at quantifying the effect of the varying amount of mineral admixtures on the rheology, setting time and compressive strength of SDC containing natural sand and crushed sand. Ordinary Portland cement (OPC) in conjunction with the mineral admixtures was used in different replacement ratio keeping the mix paste volume (35%) and water binder ratio (0.4) constant at controlled laboratory atmospheric temperature ($33^{\circ}C$ to $35^{\circ}C$). The results show that the properties and amount of fine aggregate have a strong influence on the admixture demand for similar initial workability, i.e., flow. The large amounts of fines and lower value of fineness modulus (FM) of natural sand primarily increases the yield stress of the SDC. The mineral admixtures at various replacement ratios strongly contribute to the yield stress and plastic viscosity of SDC due to inter particle friction and cohesion.

Application of the Modified Reactive SPH Method for Simulating Explosions

  • Sirotkin, Fedir V.;Yoh, Jai-Ick
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2011.11a
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    • pp.922-925
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    • 2011
  • Smoothed Particle Hydrodynamics (SPH) is a Lagrangian method widely used for the modeling fluid flows. Simulations of explosions require, besides the hydrodynamic equations, a realistic equation of state, an energy source term, and a set of chemical kinetic equations to follow the composition changes of the gas during the explosion. The performance of the hydrodynamic equations is investigated in the framework of the Sedov-Taylor blast-wave. The implementation of chemical kinetic equations and equation of state is studied with 1D detonation of TNT slab. Our results are compared to those from analytical and experimental studies.

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Solution State Structure of pA1, the Mimotopic Peptide of Apolipoprotein A-I, by NMR Spectroscopy

  • Kim, Hyo-Joon;Won, Ho-Shik
    • Bulletin of the Korean Chemical Society
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    • v.32 no.9
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    • pp.3425-3428
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    • 2011
  • Apolipoprotein A-I (Apo A-I) is a major component for high density lipoproteins (HDL). A number of mimetic peptides of Apo A-I were screened from the phase-displayed random peptide library by utilizing monoclonal antibodies (A12). Mimetic peptide for A12 epitope against Apo A-I was selected as CPFARLPVEHHDVVGL (pA1). From the BLAST search, the mimetic peptide pA1 had 40% homology with Apo A-I. As a result of the structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based distance geometry (DG)/molecular dynamic (MD) computations, DG structure had low penalty value of 0.3-0.7 ${\AA}^2$ and the total RMSD was 0.6-1.6 ${\AA}$. The mimotope pA1 exhibited characteristic conformation including a ${\beta}$-turn from Pro[7] to His[11].