Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
권호 표지
DOI QR코드

DOI QR Code

Isolation and characterization of four unrecorded wild yeasts from the soils of Republic of Korea in winter

  • Yuna Park (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Soohyun Maeng (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Sathiyaraj Srinivasan (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2021.09.14
  • Accepted : 2023.05.08
  • Published : 2023.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to isolate and identify wild yeasts from the soil collected in Gwangju and Pocheon City, Gyeonggi Province, Republic of Korea. Among 10 strains, six strains were already reported, but four strains were unrecorded in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were performed using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were carried out using API 20C AUX kit. The 10 strains were assigned to the phyla Basidiomycota (8 strains) and Ascomycota (2strains). The unrecorded four yeast strains, NH33, NH19, NH20, and YP416, belong to the phylum Basidiomycota and the genera Buckleyzyma, Leucosporidium, Holtermanniales, and Mrakia, respectively. All strains had oval-shaped and polar budding cells. In this research, the morphological and biochemical properties of four unreported yeast species were characterized intensively, which were not officially reported in Korea.

Keywords

Acknowledgement

This work was supported by a research grant from Seoul Women's University (2021) and by a grant from the National Institute of Biological Resources(NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202130202).

References

  1. Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25(17):3389-3402. https://doi.org/10.1093/nar/25.17.3389
  2. Fell, J.W., T. Boekhout, A. Fonseca, G. Scorzetti and A. Statzell-Tallman. 2000. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50(3):1351-1371. https://doi.org/10.1099/00207713-50-3-1351
  3. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791. https://doi.org/10.2307/2408678
  4. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  5. Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger data dets. Mol Biol Evol 33(7):1870-1874. https://doi.org/10.1093/molbev/msw054
  6. Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4):331-371. https://doi.org/10.1023/A:1001761008817
  7. Sampaio, J.P. 2011a. Leucosporidiella Sampaio 2003. In: Kurtzman, C.P., J.W. Fell and T. Boekhout (eds.), The yeasts, a taxonomic study, vol 3, 5th edn. Elsevier, New York. pp. 1801-1806.
  8. Sampaio, J.P. 2011b. Leucosporidium Fell, Statzell, Hunter and Phaff 1969. In: Kurtzman, C.P., J.W. Fell and T. Boekhout (eds.), The yeasts, a taxonomic study, vol 3, 5th edn. Elsevier, New York. pp. 1485-1494.
  9. Sampaio, J.P., M. Gadanho, R. Bauer and M. Weiss. 2003. Taxonomic studies in the Microbotryomycetidae: Leucosporidium golubevii sp. nov., Leucosporidiella gen. nov. and the new orders Leucosporidiales and Sporidiobolales. Mycol Prog 2:53-68. https://doi.org/10.1007/s11557-006-0044-5
  10. Tsuji, M., Y. Tanabe, W.F. Vincent and M. Uchida. 2019. Mrakia hoshinonis sp. nov., a novel psychrophilic yeast isolated from a retreating glacier on Ellesmere Island in the Canadian High Arctic. Int J Syst Evol Microbiol. 69(4):944-948. https://doi.org/10.1099/ijsem.0.003216
  11. Turchetti, B., C. Sannino, A. Mezzasoma, L. Zucconi, S. Onofri and P. Buzzini. 2020. Mrakia stelviica sp. nov. and Mrakia montana sp. nov., two novel basidiomycetous yeast species isolated from cold environments. Int J Syst Evol Microbiol 70 (8):4704-4713. https://doi.org/10.1099/ijsem.0.004336
  12. Wang, Q.M., A.M Yurkov, M. Goker, H.T. Lumbsch, S.D. Leavitt, M. Groenewald, B. Theelen, X.Z. Liu, T. Boekhout and F.Y. Bai. 2015. Phylogenetic classification of yeasts and related taxa within Pucciniomycotina. Stud Mycol 81:149-189. https://doi.org/10.1016/j.simyco.2015.12.002
  13. Wuczkowski, M., V. Passoth, B. Turchetti, A.C. Andersson, M. Olstorpe, A. Laitila, B. Theelen, M. van Broock, P. Buzzini, H. Prillinger, K. Sterflinger, J. Schnurer, T. Boekhout and D. Libkind. 2011. Description of Holtermanniella gen. nov., including Holtermanniella takashimae sp. nov. and four new combinations, and proposal of the order Holtermanniales to accommodate tremellomycetous yeasts of the Holtermannia clade. Int J Syst Evol Microbiol 61(3):680-689. https://doi.org/10.1099/ijs.0.019737-0
  14. Yurkov, A.M., C. Sannino and B. Turchetti. 2020. Mrakia fibulata sp. nov., a psychrotolerant yeast from temperate and cold habitats. Antonie Van Leeuwenhoek 113(4):499-510. https://doi.org/10.1007/s10482-019-01359-4