• 혜화의학회지
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• 제11권1호
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• pp.137-149
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• 2002

Dong-Eui-Bo-Gham Oriental medicine clinic on the purpose of losing their weight, from March 1, 2001 to March 1, 2002. The characteristics studied were gender, average weight, average height, distribution of body fat rate, base body metabolism rate, distribution of bmi, purpose of losing weight, treatment history, cause of gaining weight, clinical symptoms, distribution of Sahsang constitution, and change of body constituent. The conclusions drawn from this study were: 1. Among patients who came to the clinic for weight loss, there were 16 females (80%), and 4 males (20%). The ratio of gener was 1 to 4 (male to female) The distribution of their ages were 1 in one's teens (5%), 6 in their 20s (30%), 7 in their 30s (35%), and 6 in their 40s (30%). 2. The average weight of the patients was 72.155kg, and their average height was 163.2 cm. 3. The body fat rates of patients were distributed as: 2 were below 25 (10%), 3 were 25 to 30 (15%), 8 were 30 to 35 (40%), 5 were 35 to 40 (25%), and 2 were 40 to 45 (10%). 4. The base body metabolism rates of the patients were distributed as: 1 was below 1000Kcal (5%), 6 were 1100 to 1200Kcal (30%), 5 were 1200 to 1300Kcal (25%), 6 were 1300 to 1400Kcal (30%), and 2 were 1400 to 1500Kcal (10%). 5. The distribution of the patients bmi was: 4 were below 20 (20%), 5 were 20 to 25 (5%), 11 were 25 to 30 (55%), and 4 were 30 to 35 (20%). 6. The patients visited the clinic to lose their weight for the follwoing purposes: 5 were related to health concerns (25%), and 15 were to imprve their physical apprearnce (75%). Among patients who wanted to lose their weight becase of health concerns, 4 were married (20%) and 1 was single (5%). Among patiens who wanted to lose their weight to improve their physical appearance, 6 were married (30%) and 9 were single (45%). 7. As for the past treatment methods, physical exercises were most frequently used (19 patiens, 43%). 14 tried diet (32%), 8 used food substitues (19%), 1 was treated through Western medicine, and 1 was not related to any of these (2%). 8. Main reasons for weight gains were: 12 were related to overeating (40%), 10 were stress (33%), and 8 were lack of physical exercise (27%). 9. Physical symptoms included: 15 were fatigue (29%), 14 were constipation (26%), 13 were body swelling (25%), 3 were headache (5%), menstruation pain (4%), one was stomach upset (2%), 2 were related to physical structure, and 2 were related to other (4%). 10. As for the distribution of Sahsang constitution, 10 were Taeum (50%), 9 were Sohyang (45%), and 1 was Sohum (5%). 11. The average muscle weight was 44.87kg, average abdomen fat rate was 0.8999, and average base body metabolism rate was1369.2Kcal. 12. For the 5-week period, The body fat rate changes were 35.93 1.56% to 30.40 1.98% for A group, and 34.27 1.19% to 31.73 1.38% for B group. The abomen fat rate changes were 0.90 0.02% to 0.86 0.03 for A group, and 0.89 0.02% to 0.83 0.02% for B group. The body fat weight changes were 26.92 2.04kg to 20.74 1.98kg for A group, and 27.86 3.37kg to 24.58 3.02kg for B group. The body weight chages were 72.36 3.18kg to 64.54 2.55kg for A group, and 72.48 3.14kg to 67.74 3.11kg for B group. The muscle rate changes were 43.86 1.63kg to 43.10 1.76kg for A group, and 42.72 1.22kg to 41.60 1.86kg for B group. Overall, A group was superior to B group. 13. After being treated for obesity, 13 patients used exercise treatment (65%), 5 continued to receive obesity treatment (25%), and 2 used diet (10%). Based on these results, we could conclude that the importande of setting a criterion of obesity and weight control have changed according to changes in social and cultural values, and that treatment of obesity through Oriental medicine and research should keep up with changes in esthetic and psychological values.

• 한국임상수의학회지
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• 제29권3호
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• pp.213-219
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• 2012

재조합 bST(recombinant bovine somatotropin; rbST)는 젖소에서 건강에 부작용을 초래하지 않으면서 유생산을 증가시키는 것으로 알려졌다. 본 연구는 250 mg의 rbST를 함유한 $Boostin^{(R)}$-250 제제와 rbST를 500 mg 함유하는 $Posilac^{(R)}$ 제제와 $Boostin^{(R)}$-S 제제의 투여시 유생산에 미치는 영향과 대상동물에 대한 rbST 투여의 안전성을 비교하기 위하여 실시하였다. 젖소는 1군에서 4군까지 각각 25마리씩 임의배치하였다. $Boostin^{(R)}$-250 제제와 부형제(대조군)를 매주 투여하였으며 $Boostin^{(R)}$-S 제제와 $Posilac^{(R)}$제제는 2주 간격으로 투여하였다. 젖소의 유량, 유성분, 체세포수, 건강상태, body condition score (BCS)를 측정하였다. $Posilac^{(R)}$제제, $Boostin^{(R)}$-S 제제, $Boostin^{(R)}$-250 제제의 투여에 따른 유생산 증가는 대조군과 비교하여 각각 2.9 kg/day (12.3%), 4.2 kg/day (17.9%), 4.1 kg/day (17.4%)이었으며 rbST를 투여한 군들과 대조군 사이에서 통계적 유의차가 확인되었다. rbST의 투여는 임상형 유방염발생과 우유의 체세포수를 증가시키지 않았으며 rbST의 투여는 유성분에도 큰 영향을 미치지 않았다. 최고유량 이후 rbST의 투여는 BCS에 부정적인 영향을 미치지 않았지만 비유 100일 이내의 일부 젖소들은 rbST의 투여 후 BCS가 감소하였다. 결론적으로 rbST 250 mg의 매주 투여는 rbST 500 mg의 2주 간격 투여와 유사한 우유 증산효과를 나타내었으며 젖소의 대사성 스트레스를 감소시키는 것으로 판단되었다.

• 대한한방소아과학회지
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• 제14권2호
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• pp.149-168
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• 2000

The Purpose of this study was to investigate the new effective oriential medicine tretments in pediatric disease and its clinical applicability The study was composed of 1245 new patients who had been treated at the all unit in the Dong-Seo oriential Medicine Hospitial for 1 year, from 1 April 1999 to 31 March 2000, and aged between 0 and 18 years. The chief complaint was mainly categorized by oral examination on patients and their care-givers. Result 1. The numbers of children who involved in this study is total 1245: Male children are 668 and female children are 577 children. The sex rate between male and female is 1.15 to 1. 2. Most of the above patients came to the hospital at first time from diseases such as musculoskelectal disease, weakness, asthma, cough, anorexia, common cold, rhiorrhea, sweating, dyspepsia, dematitis, night terror, obesity, stomach, short stature, Besides, they also came to the hospital at first time from various diseases such as epistasxis, pyrexia. Bell's palsy, nocturia, contipation, cerebral palsy, disorder, CVA. diarrhea. stress disease, Allergic disease, Tic disorder. Visual disorder, Kawasaki disease, Pierre Robin's syndrom, hematuria, edema and so on. 3. Looking at the frequent diseases, Respiratory dis. children including asthma, cough, rhiorrhea, sweating, common cold, pyrexia covers 36%, in 399 numbers. digestive dis. children including anorexia, dyspepsia, stomach, diarrhea, constipation, indigestion covers 19%, in 211 numbers. cadiovasculary disease children including arrthymia, terror in frequency, night crying, sediation, Tic disorder covers 8%, in 85 numbers. Hepatobiliary disease children including short stature, dizziness, visual difficulty, sprain, disorder of nail covers 21%, in 238 numbers. renal disease. children including nocturia, hematuria, hemation, disorder of hair, menorrhea, cerebral palsy, edema in 44 numbers. Fatigue children covers 13%. in 143 numbers. 4. In case of respatory disease children. total number is 399 children. dematitis children are 108 numbers and asthma children are 96 numbers. These show that children seem to the best have dematitis and asththma. The age from 0 to 6 is 290 numbers, covering 73 percentage. The others are covering 27 percentage. These data demonstrate that the age from 0 to 6 age could easily get these kinds - of diseases. The 29 percent of children had these kinds of diseases in spring. The 28 percent of children had these kinds of diseases in autumn. These show that children seem to frequently have these kinds of diseases in both spring and autumn season.(inter-season) 5. In case of digestive disease, anorexia covers 39%, in 83 numbers, dyspepsia covers 28%, in 59 numbers, anorexia and dyspepsia were the lagest group in digestive disease and the age from 0 to 6 covers 59%, in 125 numbers and the other age covers 39%. According to these data, infant seems to be vulnerable to these kinds of diseases. The 24 percent of children had these kinds of diseases in spring and summer. The 33 percent of children had these kinds of diseases in winter, which means that children mostly had winter. but decreased significant in autumn. 6. In case of cadiovasculary diseases, it can be divided into two categories: night covers 73% and nervous characteristics covers 27%. The age from 0 to 6 occupies 75%, and the other age occupies 25%. These data also show that infants can easily infected with these kinds of diseases. Analyzing by the season, summer could be the most frequent season that children have these kinds of diseases. but decreased in autumn. 7. In case of hepatobiliary children, sprains covers 166 numbers, fatigue covers 32 numbers, epistasxia covers 24 members, the reasion which sprain occupyied most of % were sprain covered ages. The age from 0 to 14 covers 59% and from 15 to 18 covers 41%. In the conclusion the adolescent seems to be vulnerable to sprains. In spring 29%, insummer 31%, in autumn 23%, and in winter 28% of children got these kinds of diseases, which show that children seems to have this kinds of disease in summer season. and decreased in autumn. 8. In case of renal disease. nocturia and hematuria covers 52%(occupied overhalf). The age from 0 to 6 covers 52%(occupied overhalf). Analyzing by season, in spring increased in summer(59%), decreased in autumn(45%) Conclusion 1. The chief complant in pediatric diseases that needed an oriental medical tretment was mainly the disease that tends to take iong time and the weakness. and appeared frequency in respiratory disease : 2. The oriential medical tretment was still preferred as a way to improve the weakness by patients, rather than a way to overcome their disease. In paticular, the study shows that the oriental medical tretment should be emphasized in terms of preventing the disease 3, The new disease, which were developed with the change of human life and envir oment(just like seual disorder, short stature, obesity, dynamic disorder, examinee disease), should be in vestigated as a new field of oriental medical tretment.

• 대한관절경학회지
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• 제16권2호
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• pp.114-120
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• 2012

목적: 내측 반월상 연골 후각 부착부 봉합술 시 시행하는 표층 내측측부인대 유리술의 유용성 및 안전성에 대해 연구하고자 하였다. 대상 및 방법: 2006년 4월부터 2010년 9월까지 표층 내측측부인대 유리술을 시행하지 않고 내측 반월상 연골 후각 부착부 봉합술을 시행한 환자 20명(PM군)과 표층 내측측부인대 유리술을 시행하고 봉합술을 시행한 시행한 환자 32명(RM군)을 대상으로 하였다. 동반 수술을 시행한 환자는 제외하였다. 안정성 검사로 술 후 3개월, 1년 추시 시 압통, 주관적 불안정성 및 외반 부하 검사를 시행하였고, 유용성 검사로 압박대 사용시간을 비교하였고, 임상 결과는 Lysholm 점수와 International Knee Documentation Committee (IKDC) 점수로 평가하였다. 결과: 전례에서 표층 내측측부인대 유리술과 관련하여 임상적으로 의미 있는 합병증은 없었다. 술 후 3개월 및 1년 추시 관찰 시 압통, 주관적 불안정성에서 음성을 보였으며, 5명의 환자에서 외반 부하 검사상 grade I의 불안정성을 보였다. 압박대 사용 시간은 PM군 $53.5{\pm}13.6$분, RM군 $41.3{\pm}12.7$분으로 두 군간 차이를 보였다(P<0.05). 술 후 임상결과는 PM군은 술 전 Lysholm 점수 $56.8{\pm}5.5$에서 술 후 $85.1{\pm}5.8$로(P<0.001), 술 전 IKDC 점수 $42.6{\pm}6.3$에서 술 후 $77.2{\pm}6.3$로(P<0.001), RM군은 술 전 Lysholm 점수 $56.2{\pm}5.4$에서 술 후 $87.4{\pm}3.9$로(P<0.001), 술 전 IKDC 점수 $42.7{\pm}5.7$에서 술 후 $89.6{\pm}2.9$로(P<0.001) 개선되었다. 두 군간 수술 전, 후의 Lysholm 점수, IKDC 점수 차이는 없었다(P>0.05). 결론: 표층 내측측부인대 유리술은 슬관절 내측 수술 시 시야 확보에 유리하며, 수술 시간을 줄일 수 있고, 임상적 의미있는 잔존 불안정성을 남기지 않아 내측 반월상 연골 후각 부착부 봉합술 시 유용한 것으로 사료된다.

• Clinical and Experimental Pediatrics
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• 제48권2호
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• pp.143-147
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• 2005

목 적 : 갑상선 기능은 여러 질환이나 스트레스에 의해서 영향을 받는 것으로 알려져 있다. 저자들은 분만 전과 분만 동안의 여러 인자와 제대혈 갑상선자극호르몬 및 갑상선호르몬 농도와의 상관관계를 알아보고자 본 연구를 시행하였다. 방 법 : 총 130명의 신생아를 대상으로 분만 즉시 제대혈을 10 mL 채취하여 갑상선자극호르몬, $T_3$와 유리 $T_4$ 농도를 방사면역학적 방법(CIS bio international kit, Germany)으로 측정하였다. 재태연령, 출생체중, 가사, 분만방식, 산모의 당뇨병 유무, 산모의 전자간증 유무 등에 따라 갑상선자극호르몬과 갑상선 호르몬 농도를 비교하였다. 결 과 : 1) 제대혈 갑상선자극호르몬 농도는 재태연령 34주 이하 $1.73{\pm}0.48{\mu}IU/mL$, 34주-37주 $2.60{\pm}0.51{\mu}IU/mL$, 38주 이상 $4.26{\pm}0.40{\mu}IU/mL$으로 재태연령의 증가에 따라 증가하였다(P<0.05). 2) 분만형태를 비교하면 질식 분만 군 $4.42{\pm}0.66{\mu}IU/mL$, 제왕절개 분만 군 $3.31{\pm}0.33{\mu}IU/mL$로 질식 분만 군에서 높았다(P<0.05). 3) 가사에 따른 갑상선자극호르몬 농도는 가사가 있는 군 $5.18{\pm}0.93{\mu}IU/mL$로 가사가 없는 군 $2.97{\pm}0.84{\mu}IU/mL$에 비해 유의하게 높았다(P<0.05). 4) 산모의 당뇨병에 따른 갑상선자극호르몬 농도는 당뇨병 군 $8.91{\pm}1.25{\mu}IU/mL$, 없는 군 $4.32{\pm}0.42{\mu}IU/mL$으로 산모 당뇨병 동반시 유의하게 높았다(P<0.05). 5) 산모에게 전자간증이 있는 군의 갑상선자극호르몬 농도는 $5.28{\pm}0.42{\mu}IU/mL$, 없는 군 $3.65{\pm}0.46{\mu}IU/mL$에 비해 유의하게 높았다(P<0.05). 6) $T_3$와 유리 $T_4$ 농도는 가사 군에서 없는 군보다 유의하게 낮았다(P<0.05). 7) 각 변수 간의 영향을 배제하였을때 임신주수, 1분 Apgar 점수, 산모의 당뇨병만이 독립적으로 제대혈의 갑상선자극호르몬 농도에 영향을 미치는 것으로 나타났다. 결 론 : 제대혈 갑상선자극호르몬 및 갑상선호르몬 농도는 산모의 당뇨병이나 산모 전자간증과 같은 분만 전 요인과 태아에게 저산소증을 초래할 수 있는 분만시 스트레스와 밀접한 연관이 있다.

• 정신신체의학
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• 제27권2호
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• pp.77-84
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• 2019

연구목적 질병침습도 평가 척도(Illness Intrusiveness Rating Scale)란, 만성 질환을 앓는 환자에서 질병이나 치료가 삶의 질에 얼마나 영향을 미치는지 평가하기 위한 타당도가 입증된 자가보고식 검사이다. 여러 연구를 통하여 만성 질환자에서 요인 모델의 적합도가 보고되었으나, 정신 질환자를 대상으로는 그 요인 타당도가 확인되지 않았다. 본 연구는 불안 및 우울장애가 주요 진단인 정신건강의학과 외래환자 군을 대상으로 한국판 질병침습도 평가 척도(Illness Intrusiveness Rating Scale-Korean version, IIRS-K)의 요인 구조를 확인하고, 요인 타당도를 조사하기 위하여 진행되었다. 방 법 2010년부터 2013년까지 3년간 한양대학교 구리병원 정신건강의학과 외래 초진 환자를 연속적 수집하여 총 307명의 자료를 분석하였다. IIRS-K, Zung 자가평가 우울척도, Zung 자가평가 불안척도를 이용하였다. Cronbach's α계수를 통해 신뢰도를 분석하였고, Varimax 직각회전을 이용한 탐색적 주성분 분석과 최대우도법 중 보수적인 방법을 추정자(standard maximum likelihood estimator)로 확인적 요인분석을 시행하였다. IIRS-K의 하위 요인과 우울, 불안 증상간의 타당도는 Spearman 상관 분석으로 조사하였다. 결 과 IIRS-K의 내적 일치도는 0.9로 높은 신뢰도를 보였다. 13개 문항에서 3개 요인이 추출되었고, 총 분산의 63.2%를 설명하여, 58.3%를 설명한 원본 영문판과 유사한 요인 구조를 보였다. 3요인 분석이 가장 모델에 적합한 것을 확인적 요인분석으로 확인하였으며, 우울 및 불안 증상과도 정적인 상관관계를 보여 수렴 타당도를 입증하였다. 결 론 IIRS-K의 3요인구조는 조용한 취미 활동, 개인 발전 등 일부 문항 구성에서 원본과 차이를 보였고, 가족 관계나 배우자와의 관계, 경제 문제가 한 요인에 추출되는 것이 특징적이었다. 이는 문화적 특징을 반영한 결과라고 볼 수 있다. 이 연구는 정신건강의학과 임상군을 대상으로 IIRS-K의 요인 타당도를 입증한 첫 연구이며, 이를 통해 국내 정신건강의학과 환자의 삶의 질에 사용될 유용한 도구를 제공했다는 의의가 있다.

• 대한핵의학회지
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• 제28권2호
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• pp.177-185
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• 1994

Pharmacologic coronary vasodilation in conjunction with myocardial perfusion scintigraphy has become an alternative to dynamic exercise test for the diagnosis and risk stratification of coronary artery disease, especially in patients who are unable to perform adequate exercise. Dipyridamole and adenosine have been used for pharmacologic stress testing with myocardial perfusion imaging. Adenosine is a potent coronary vasodilator with rapid onset of action, short half-life, near maximal coronary vasodilation and less serious side effects. ST segment depression has been reported in about 7-15% of patients with coronary artery disease receiving dipyridamole in conjunction with myocardial perfusion imaging. The exact cause and clinical significance are not known. In order to evaluate the relationship between adenosine-induced ST segment depression during $^{99m}Tc$-MIBI myocardial perfusion scintigraphy and the severity of coronary artery disease, we performed $^{99m}Tc$-MIBI imaging after intravenous Infusion of adenosine In 120 patients with suspected coronary artery disease. Of the 120 patients, 28 also performed coronary angiography. There were 24 patients with ST segment depression during $^{99m}Tc$-MIBI scintigraphy and 96 patients without ST segment depression. Adenosine was infused Intravenously at a dose of 0.14mg/kg per minute lot 6minutes and $^{99m}Tc$-MIBI was injected at 3 minute. We then com-pared the hemodynamic changes, side effects, scintigraphic and angiographic findings. Heart rate increased $90{\pm}19$ beats/minute in the group with ST depression compared with $80{\pm}16$ beats/minute in the group without ST depression(p<0.05). Baseline systolic blood pressure was significantly higher in the group with ST depression($152{\pm}27$ mmHg) than in the group without 57 depression($140{\pm}21$mmHg, p<0.05). Double product at baseline($10.90{\pm}2.77$ versus $9.55{\pm}2.34\;beats/minute{\times}mmHg$) and during adenosine infusion($12.72{\pm}3.89$ versus $10.83{\pm}2.98\;beats/minute{\times}mmHg$) were significantly higher in the group with ST depression(p<0.05). The incidence of anginal chest pain was also significantly higher in the group with ST depression(ST versus 29%, p<0.0001). The $^{99m}Tc$-MIBI images were abnormal in 23(96%) patients with ST segment depression and 66(69%) patients without ST segment depression(p<0.05). In patients with ST segment depression, there were more reversible perfusion defects than in patients without ST segment depression(83 versus 55%, p<0.05). The number of abnormal segments were significantly higher in the group with ST depression($3.05{\pm}2.01$ versus $1.51{\pm}1.45$, p<0.005). In patients with ST segment depression, there were more segments of reversible perfusion defects than in patients without segment depression($2.15{\pm}2.11$ versus $0.89{\pm}1.24$, p<0.05). There were no differences in the angiographic severity by vessel(p ; NS). We concluded that ST segment depression during $^{99m}Tc$-MIBI myocardial perfusion scintigraphy with Intravenous adenosine is related to the severity of coronary artery disease.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제27권5호
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• pp.373-384
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• 2001

Cellular proliferation is an intricately regulated process mediated by the coordinated interactions of critical growth control genes. Two of these factors in mammalian cells are the p53 and mdm-2 genes. A protein product of the mem-2 oncogene has been recently shown to associate with the protein encoded by the tumor suppressor gene p53. The p53 tumor suppressor protein is stabilized in response to DNA damage and other stress signals and causes the cell to undergo growth arrest or apoptosis, thus preventing the establishment of mutations in future cellular generations. Mutation or loss of p53 is a very common event in tumor progression. It occurs in about 50% of all tumors analysed including of colon, lung, breast and liver. The cellular mdm-2 gene, which has potential transforming activity that can be activated by overexpression, is amplified in a significant percentage of human sarcoma and in other mammalian tumors. Proteins encoded by the mdm-2 gene are able to bind to the p53 protein and, when overexpressed, can inhibit p53's transcriptional activation function, thus mdm-2 can act as a negative regulator of p53 function. Experimental study was performed to observe the relationship between p53 gene mutation and mdm-2 protein expression and apply the results to the clinical activity. 36 golden syrian hamster each weighing $60{\sim}80g$ were used and painted with 0.5% DMBA by 3 times weekly on the right buccal cheek(experimental side) for 6, 8, 10, 12, 14 and 16 weeks. Left buccal cheek(control side) was treated with mineral oil as the same manner to the right side. The hamsters were sacrificed on the 6, 8, 10, 12, 14 & 16 weeks. Normal and tumor tissues from paraffin block were examined for histology and immunohistochemistry observation, and were completely dissected by microdissection and DNA from both tissue were isolated by proteins K/phenol/chloroform extraction. Segments of the hamster p53 exons 5, 6, 7 and 8 were amplified by PCR using the oligonucleotide primers, and then confirmational change was observed by SSCP respectively. The results were as follows : 1. Dysplasia at 6 weeks, carcinoma in situ at 8 weeks and invasive carcinoma from 10 weeks could be observed in experimental groups. 2. p53 mutations were detected in 10 of the 36(28%) and the exons 6(6 of the 10 : 60%) was the most hot spot area among the highy conserved region(exons 5, 6, 7 & 8). 3. Immunohistochemical study confirmed 22 of the 36(61%) of p53 expression involving 10 of p53 mutations. 4. mdm-2 expression of was showed in 3 of the 36(8%) involving 1 of the 22 of p53 expression and 2 of the 14 of p53 non-expression. From the above results, mutation of p53 gene or expression of p53 protein may have the influence of the DMBA induced carcinoma of hamster buccal pouch but the expression of mdm-2 protein may not have relationship with tumorigenesis.

• 대한소아치과학회지
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• 제29권2호
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• pp.243-254
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• 2002

소아치과 임상에 흔히 사용되는 3종의 금속재에 의한 인체 섬유모세포(HGF-1)를 이용하여 세포독성에 대한 세포적 및 분자적 접근을 시도하였다. 세포의 성장과 증식 및 세포사, 그리고 이들과 관련된 세포내 신호전달물질 발현의 변화를 보고자 하였고, 세포사는 screening한 후 세포사의 유형(necrosis/apoptosis)을 규명하고자 하였다. HGF-1은 DMEM으로 계대배양한 후 유지하였으며, 사용한 금속재는 stainless steel crown (R), 교정용 band(B), 교정용 wire(W)의 세 종류였다. HGF-1세포의 증식에 대하여 기본적으로 cell count를 하였고, 생존세포 count를 위하여 대조군에 대한 실험군의 상대증식도를 계산하였다. 세포수는 배양일이 지남에 따라 증가하여 7일째에 최고를 나타내었고, 11일째에는 감소하였으며, 대조군과 실험군 사이의 통계적 유의한 차이는 관찰되지 않았다(p>0.05). 대조군에 대한 실험군의 상대증식도 또한 유의한 차이는 없었다(p>0.05). 세포사에 대한 형태적 소견은 괴사(necrosis)를 나타내었다. PCNA lableing index에 대한 대조군과 실험군간에 유의한 차이는 없었다(p>0.05). 신호전달 관련 MAP kinase인 ERK1 및 ERK2, p38, JNK는 배양일의 경과에 따른 발현의 변화는 관찰되었으나, 대조군과 각 실험군 간에 차이는 관찰되지 않았다. 결론적으로 본 연구대상인 stainless steel crown, 교정용 band 와 wire, 세 종류의 소아 치과용 금속재는 단기간의 인체섬유모세포를 이용한 세포친화성 검정에 있어서 세포적, 분자적 위해 양상을 나타내지 않았다.

• 동국한의학연구소논문집
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• 제4권
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• pp.161-187
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• 1995

This study was perfomed to investigate causes of the senile hypotension, pathogenic mechanism, symptoms, and therapies through medical literatures, recent chinese medical literatures and chinese medical journals. The results are as follows ; 1. The senile hypotension has major symptoms of dizziness, weakness, syncope, palpitation, shortness of breath, and deficiency of Qi. Additionally, it has minor symtoms of letharhy, isomnia, tinnitus, amnesia etc... 2. The prodromal symptoms of Kwul and Kwul are relating to the symptoms of tachycardia, facial pallor, sweating, anxietas, ambiguous consciousness, and fainting. Weakness and dizziness due to deficiency make the symptoms of exhaustion, fatigue, vertigo, lethargy, and brachycardia. 3. The most principal cause of the senile hypotension is deficiency of Shen due to aging, congenital deficiency, and chronic illness. The rest of causes are defciency of Qi and blood, phlegm of retention, stagnation of Qi, blood stasis, blood prostration etc... In the view of the occidental meicine, the causes of the senile hypotension came from the reduction of cardiac output, the decretion of cardiovascular system's extention due to aging, hereditary factor, secondary factor due to exsanguination, diabetes mellitus, C.V.A etc..., and factor of neurogenic system's degeneration. 4. The principal pathogenic mechanisms are the insufficiency of Xing-Yang, the deficiency of Qi in middle jiao, and deficiency of Shen-Qi. The rest of mechanisms are the deficiency of both Qi and blood, stagnation of the Gan-Qi, and the deficiency of Gan and Shen. Zang-Pu Organs have something to do with Xing, Bi, and Shen. 5. As principal therapies, there are warming and recuperation the Xing-Yang, strengthing the middle-jiao and replenishing Qi, replenishing vital essence to tonify the Shen, and warming and recuperation the Shen-Yang. Additionally, the therapies of invigorating the Bi and relieving mental stress, strengthning the Bi and tonifing the Shen, invigorating Qi and nourishing Yin, soothing the Gan and regulating the circulation of Qi, and tonifing the Shen and nourishing the Gan help the cure of the senile hypotension. In prescriptions there are Baohe Yuan Tang, Buzhong Yigi Tang, Zuoguei Yin, Yougui Yin, Guipi Tang, Zhu Fu Tang, Shengmai San, Sini San, and Qi Ju Dihuang Wan. The medical herbs of Astragali Radix, Codonopsitis Pilosulae Radix, Ginseng Radix, Aconiti Tuber, Ephedrae Herba, Cinnamomi Ramulus, Cinnamomi Corfex Spissus, Zingiberis Rhizoma, Polygalae Radix, Liriopis Tuber, Polygonati Sibirici Rhifoma, Lycii Fructus, Schizandrae Fructus, and Glycyrrhizae Radix can be treated. 6. According to the clinical report, the principal causes are the deficiency of Qi, and insufficiency of Yang which symptoms are dizziness, vitality fatigue and acratia, amnesia, body cold and alger of extremity, spontaneous perspiration, and therady and weak pulse. It was improved by taking WenYang YiQi Tang, Zhu Fu Tang about 20-30 days. The improvement was shown on disappearance of subjective symptoms or the ascending of blood pressure to normal figure, and the rate of improvement was over 70%. 7. As regimens, taking warming and recuperating food(a sheep mutton, juglans regia, chiness date, longan aril etc...) and pungent food(chinese green onion, fress ginger, pipers fructns etc...), doing physical training, not being ill in bed at a long time, and preventing descent of blood pressure coming from sudden change of posture are needed. Additionally, the usage of diuretic, abirritant, and depressor needs to be extra cautious.