• International Neurourology Journal
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• v.22 no.4
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• pp.260-267
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• 2018

Purpose: A major question remaining in approaches to tissue engineering and organ replacement is the role of native mobilized native cells in the regeneration process of damaged tissues and organs. The goal of this study was to compare the cell mobilizing effects of the chemokine CXCL12 and cell therapy on the urinary sphincter of nonhuman primates (NHP) with chronic intrinsic urinary sphincter dysfunction. Methods: Either autologous lenti-M-cherry labeled skeletal muscle precursor cells (skMPCs) or CXCL12 were injected directly into the sphincter complex of female NHPs with or without surgery-induced chronic urinary sphincter dysfunction (n=4/treatment condition). All monkeys had partial bone marrow transplantation with autologous lenti-green fluorescent protein (GFP) bone marrow cells prior to treatment. Labeled cells were identified, characterized and quantified using computer-assisted immunohistochemistry 6 months posttreatment. Results: GFP-labeled bone marrow cells (BMCs) were identified in the bone marrow and both BMCs and skMPCs were found in the urinary sphincter at 6-month postinjection. BMCs and skMPCs were present in the striated muscle, smooth muscle, and lamina propria/urothelium of the sphincter tissue. Sphincter injury increased the sphincter content of BMCs when analyzed 6-month postinjection. CXCL12 treatment, but not skMPCs, increased the number of BMCs in all layers of the sphincter complex (P<0.05). CXCL12 only modestly (P=0.15) increased the number of skMPCs in the sphincter complex. Conclusions: This dual labeling methodology now provides us with the tools to measure the relative number of locally injected cells versus bone marrow transplanted cells. The results of this study suggest that CXCL12 promotes mobilization of cells to the sphincter, which may contribute more to sphincter regeneration than injected cells.

• Korean Chemical Engineering Research
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• v.60 no.2
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• pp.289-294
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• 2022

Oil contained in domestic and industrial wastewater or marine spilled oil gives rise to severe environmental pollution issues such as water pollution and ecosystem destruction. The membrane filtration method as one of representative oil/water separation strategies has technological challenges such as membrane fouling and low separation rate. In this work, we devise a 3D-printed microhydrocyclone for oil/water separation by utilizing a digital lighting processing-based 3D printer. We demonstrate that the 3D-printed microhydrocyclone can effectively separate oil and water phases from oil-in-water emulsion.

• Journal of Plant Biotechnology
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• v.49 no.2
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• pp.139-144
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• 2022

The genus Prunus (family: Rosaceae) consists of over 400 plant species and exhibits vast biodiversity worldwide. Given the wide distribution of this genus, its taxonomic classification is important. Anatomical characteristics are conserved and stable and can therefore be used as an important tool for the taxonomic characterization of plants. Therefore, this study aimed to assess and document the anatomical characteristics of the leaf, stem, and seed of P. africana using micrographs and photographs for possible use in the identification, quality control, and phylogenetic analysis of the species. The anatomical sections of a young stem revealed a cortex consisting of isodiametric parenchyma cells, druse crystals, primary vascular bundles, and pith. The mature stem bark majorly consisted of the rhytidome, with the periderm densely arranged in multiple layers; a cluster of stone cells; and sclerenchyma. The leaf sections were hypostomatic, with stomata sizes ranging from 18.90-(22.34)-26.90 × 15.41-(18.40)-21.22 ㎛. The leaf sections showed the presence of characteristic druse crystals, vascular bundles, and mesophyll layers. The pericarp contained the epicarp, mesocarp, and endocarp, with their thickness being approximately 350-400, 300-350, and 30-50 ㎛, respectively. In addition, it contained a seed testa with a thickness of approximately 50-60 ㎛. The morphological and anatomical characteristics observed in P. africana leaves, stems, and seeds in this study could serve as useful data for the taxonomic identification of this species.

• Journal of the Korean Wood Science and Technology
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• v.31 no.5
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• pp.57-64
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• 2003

Small cherry specimens modified with acetic anhydride and formaldehyde vapor phase were weathered by outdoor exposure and accelerated conditioning in a climate chamber. The effects of the chemical modification were evaluated by measuring their weight percentage gains (WPG), hygroscopicity and color differences before and after weathering. The average WPGs of the 72 hour acetylated and formaldehyde-treated specimens were 8.1 and 15.7%, respectively. After outdoor exposure for more than 2 months, the acetylated specimens lost weights by only 1.5%, but the formaldehyde-treated did much more than the formers. It was revealed that acetylation reduced the hygroscopicity and discoloration of wood while formaldehyde treatment didn't. Moreover the longer is the formaldehyde treatment time the more degraded after weathering. It was concluded that the vapor-phase acetylation could be applied for improving the dimensional stability of old wooden blocks.

• Journal of the Korean Wood Science and Technology
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• v.31 no.1
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• pp.10-15
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• 2003

This study was carried out to investigate a radial distribution of moisture contents(MCs) within a log cross section(LC) during oven-drying of circumferential slices cut from some of domestic hardwoods LCs. For zelkova, the slices from sapwood and from transition part already reached to below hygroscopic range at 30% in average MC, whereas the innermost slice represented about 40% MC. A moisture gradient between the innermost slice and the adjacent slice from a cherry LC became severer with drying times. For castor aralia, the green MC for heartwood slices was approximately 23% higher than that for sapwood slices, and the similar moisture gradient still lasted until average 15% MC during oven-drying. For Chinese mahogany, the MCs of the mixed slices and the innermost slice were lower than those of the slices cut from the other parts during all over drying stage.

• The Plant Pathology Journal
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• v.39 no.2
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• pp.191-206
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• 2023

Ground cherry (Physalis pubescens) is the most prominent species in the Solanaceae family due to its nutritional content, and prospective health advantages. It is grown all over the world, but notably in northern China. In 2019 firstly bacterial leaf spot (BLS) disease was identified on P. pubescens in China that caused by both BLS pathogens Xanthomonas euvesicatoria pv. euvesicatoria resulted in substantial monetary losses. Here, we compared whole genome sequences of X. euvesicatoria to other Xanthomonas species that caused BLS diseases for high similarities and dissimilarities in genomic sequences through average nucleotide identity (ANI) and BLAST comparison. Molecular techniques and phylogenetic trees were adopted to detect X. euvesicatoria on P. pubescens using recQ, hrpB1, and hrpB2 genes for efficient and precise identification. For rapid molecular detection of X. euvesicatoria, loop-mediated isothermal amplification, polymerase chain reaction (PCR), and real-time PCR techniques were used. Whole genome comparison results showed that the genome of X. euvesicatoria was more closely relative to X. perforans than X. vesicatoria, and X. gardneri with 98%, 84%, and 86% ANI, respectively. All infected leaves of P. pubescens found positive amplification, and negative controls did not show amplification. The findings of evolutionary history revealed that isolated strains XeC10RQ, XeH9RQ, XeA10RQ, and XeB10RQ that originated from China were closely relative and highly homologous to the X. euvesicatoria. This research provides information to researchers on genomic variation in BLS pathogens, and further molecular evolution and identification of X. euvesicatoria using the unique target recQ gene through advance molecular approaches.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.62-62
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• 2020

체리나무는 앵두나무아과(Drupaceae) 벚나무속(Prunus) 앵두나무아속에 속하며 유럽 중남부와 소아시아가 원산지이다. 특히 온대지역에 잘 적응하는 체리나무는 재배기간 중 비가 적은 건조한 기후에 적합하다. 최근 국내에서 체리 소비가 급격히 증가함에 따라 체리 수입량[6,454톤('11)→15,855톤('19)]이 꾸준히 증가하고 있는 추세이다. 또한 국내에서 체리나무 재배에 대한 관심이 높아지면서 농경지 및 산지에 식재되어 생산량[289톤('09)→656톤('14)] 및 재배면적[60ha('08)→144ha('14)]도 꾸준히 증가하고 있다. 그러나 체리나무의 산지재배관련 연구는 전무한 실정으로 고품질 체리의 안정적 생산이 가능한 재배기술 개발이 시급히 요구되고 있다. 따라서 본 연구는 산지재배 체리나무의 생육, 결실 및 과실품질에 대한 기초자료를 확보하기 위하여 실시하였다. 공시품종은 '라핀(Lapins)'으로 대목은 '기셀라 6(Gisela 6)'을 사용한 3년과 4년생 체리나무를 조사하였다. 체리나무의 생육특성 조사 결과, 수고 및 근원경은 3년생 체리나무 234.0±23.0 cm, 5.1±0.7 cm로 나타났고 4년생 체리나무는 346.0±27.0 cm, 7.3±0.9 cm으로 조사되었다. 본당 착과수는 3년생 체리나무의 경우 과실이 착과되지 않았고 4년생 체리나무는 563.6±42.9개로 조사되었다. 4년생 체리나무의 과실특성을 조사한 결과, 입중 8.1±0.9 g, 당도 16.3 °Brix, 경도 9.8 N, 산도 1.3%로 조사되었으며, 본당 수확량은 4.6±0.3 kg으로 예측할 수 있었다.

• Journal of Advanced Technology Convergence
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• v.3 no.2
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• pp.39-44
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• 2024

This study was prepared for a natural aroma solution for the purpose of improving depression, anxiety, and insomnia caused by stress in modern people, and applied the solution to wet tissues. Specifically, the pine oil solution was prepared by mixing Korean pine oil and black cherry oil. Heavy metal analysis, antibacterial analysis, psychological evaluation, and skin evaluation were performed on the prepared pine oil solution to examine the possibility of manufacturing functional aroma wet tissues for stress improvement. Furthermore, as a result of investigating whether pine oil solution wet tissues improved the skin, it was found that there was an improvement effect in terms of moisturizing and elasticity.

• Korean Journal of Radiology
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• v.25 no.4
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• pp.331-342
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• 2024

The Asian Society of Cardiovascular Imaging-Practical Tutorial (ASCI-PT) is an instructional initiative of the ASCI School designed to enhance educational standards. In 2021, the ASCI-PT was convened with the goal of formulating a consensus statement on the assessment of coronary stenosis and coronary plaque using coronary CT angiography (CCTA). Nineteen experts from four countries conducted thorough reviews of current guidelines and deliberated on eight key issues to refine the process and improve the clarity of reporting CCTA findings. The experts engaged in both online and on-site sessions to establish a unified agreement. This document presents a summary of the ASCI-PT 2021 deliberations and offers a comprehensive consensus statement on the evaluation of coronary stenosis and coronary plaque in CCTA.

• Journal of the Korean Society of Radiology
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• v.84 no.1
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• pp.298-303
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• 2023

Electronic cigarette or vaping-associated lung injury (EVALI) is a disease defined by lung injuries caused by e-cigarette use. It predominantly manifests in forms of organized pneumonia or diffuse alveolar damage but rarely as acute eosinophilic pneumonia (AEP). This report describes a 34-year-old male with acute respiratory symptoms and a vaping history of only nicotine. Chest CT revealed peripheral distributing multiple patchy consolidations and ground-glass opacities dominant in both lower lobes, bilateral diffuse interlobular septal thickening, and bilateral pleural effusion without cardiomegaly. Bronchoalveolar lavage fluids showed increased eosinophilia levels, while infectious laboratory results were all negative, enabling the diagnosis of both AEP and EVALI. Herein, we report a rare case of only-nicotine vaping EVALI manifested as AEP.