• Journal of Life Science
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• v.26 no.10
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• pp.1214-1217
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• 2016

Rebamipide is a mucosal-protective antiulcer drug, but its mechanism of action in gastric cancer remains elusive. CagA, a major virulence factor of Helicobacter pylori (H. pylori), is associated with the risk of gastric cancer. CagA protein is injected into gastric epithelial cells and deregulates a variety of cellular signaling molecules. CagA from H. pylori induces phospholipase D1 (PLD1) expression through NFκB activation in gastric epithelial cells, followed by invasion and proliferation of gastric epithelial cancer cells. Infection with cagA-positive H. pylori and expression of CagA enhances the binding of NFκB to the PLD1 promoter. Rebamipide abolishes H. pylori cagA-induced PLD1 expression via inhibition of binding of NFκB to the PLD1 promoter and also inhibits PLD activity. Moreover, rebamipide abolishes H. pylori CagA-induced β-catenin and the expression of a target cancer stem cell (CSC) marker gene via upregulation of miRNA-320a and -4496, followed by attenuation of self-renewal capacity of H. pylori CagA-infected gastric CSCs. In addition, rebamipide increases the chemosensitivity of CagA-expressed gastric CSCs and suppresses gastric carcinogenesis. Thus, it is speculated that rebamipide might show a potent efficacy as chemotherapeutic drug against gastric cancer cells. In this review, we summarizes recent results regarding the novel insights for the efficacy of rebamipide in gastric cancer cells.

• Molecules and Cells
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• v.19 no.1
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• pp.46-53
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• 2005

Human embryonic stem (hES) cells, unlike most cells derived from adult or fetal human tissues, represent a potentially unlimited source of various cell types for basic clinical research. To meet the increased demand for characterized hES cell lines, we established and characterized nine new lines obtained from frozen-thawed pronucleus-stage embryos. In addition, we improved the derivation efficiency from inner cell masses (to 47.4%) and optimized culture conditions for undifferentiated hES cells. After these cell lines had been maintained for over a year in vitro, they were characterized comprehensively for expression of markers of undifferentiated hES cells, karyotype, and in vitro/in vivo differentiation capacity. All of the cell lines were pluripotent, and one cell line was trisomic for chromosome 3. Improved culture techniques for hES cells should make them a good source for diverse applications in regenerative medicine, but further investigation is needed of their basic biology.

• Molecules and Cells
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• v.37 no.6
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• pp.497-502
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• 2014

Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.4
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• pp.193-200
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• 2013

Recently, network coding has been actively studied to increase the spectral efficiency of two-way relay wireless channels such as cellular systems as well as broadcasting systems. In this paper, we derive the average transmission capacity and the outage probability of a network coding system, which utilizes two-way data transmission via the best relay rather than multiple relays. Since the data unbalance between the forward and the reverse link in two-way communication systems exists, we include the asymmetric link as well as the symmetric link in the analysis. It is noticed that the space diversity gain increases as the increase of the number of relays. Also we obtain 11.4 dB signal-to-noise ratio (SNR) gain with 9 relays compared to that with single relay in symmetrical link at the given conditions. In asymmetrical links, we denotes that the outage probability is more sensitive to the number of relays rather than data unbalance between the links.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.4A
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• pp.296-303
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• 2005

In this paper, we evaluate system performance and propose signal separation and detection when a user with one antenna shares the co-channel together with a user with two space-time coded antennas. The proposed technique can identify co-channel users by an interference cancellation method and detect the signals by maximum likelihood method. Simulation results show that the shortcoming of the Minimum Mean-Squared Error technique which can be applied two users with the same number of antenna but can not applied for heterogeneous MIMO users with the different number of antennas. Also, we apply the proposed scheme to OFDM system and evaluate the system performance. By simulations, we identify that the performance of the proposed system is the same as that of the existing single antenna users and improves the performance of the two-antenna MIMO users.

• Molecules and Cells
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• v.22 no.3
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• pp.328-335
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• 2006

Imperatoxin A ($IpTx_a$), a 3.7 kDa peptide from the African scorpion Pandinus imperator, is an agonist of the skeletal muscle ryanodine receptor (RyR1). In order to study the structure of the toxin and its effect on RyR1, $IpTx_a$ cDNA was PCR-amplified using 3 pairs of primers, and the toxin was expressed in E. coli. The toxin was further purified by chromatography, and various point mutants in which basic amino acids were substituted by alanine were prepared by site-directed mutagenesis. Studies of single channel properties by the planar lipid bilayer method showed that the recombinant $IpTx_a$ was identical to the synthetic $IpTx_a$ with respect to high-performance liquid chromatography mobility, amino acid composition and specific effects on RyR1. Mutations of certain basic amino acids ($Lys^{19}$, $Arg^{23}$, and $Arg^{33}$) dramatically reduced the capacity of the peptide to activate RyRs. A subconductance state predominated when $Lys^8$ was substituted with alanine. These results suggest that some basic amino acid residues in $IpTx_a$ are important for activation of RyR1, and that $Lys^8$ plays an important role in regulating the gating mode of RyR1.

• Molecules and Cells
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• v.40 no.8
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• pp.558-566
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• 2017

Regular monitoring on experimental animal management found the fluctuation of ART outcome, which showed a necessity to explore whether superovulation treatment is responsible for such unexpected outcome. This study was subsequently conducted to examine whether superovulation treatment can preserve ultrastructural integrity and developmental competence of oocytes following oocyte activation and embryo culture. A randomized study using mouse model was designed and in vitro development (experiment 1), ultrastructural morphology (experiment 2) and functional integrity of the oocytes (experiment 3) retrieved after PMSG/hCG injection (superovulation group) or not (natural ovulation; control group) were evaluated. In experiment 1, more oocytes were retrieved following superovulation than following natural ovulation, but natural ovulation yielded higher (p < 0.0563) maturation rate than superovulation. The capacity of mature oocytes to form pronucleus and to develop into blastocysts in vitro was similar. In experiment 2, a notable (p < 0.0186) increase in mitochondrial deformity, characterized by the formation of vacuolated mitochondria, was detected in the superovulation group. Multivesicular body formation was also increased, whereas early endosome formation was significantly decreased. No obvious changes in other microorganelles, however, were detected, which included the formation and distribution of mitochondria, cortical granules, microvilli, and smooth and rough endoplasmic reticulum. In experiment 3, significant decreases in mitochondrial activity, ATP production and dextran uptake were detected in the superovulation group. In conclusion, superovulation treatment may change both maturational status and functional and ultrastuctural integrity of oocytes. Superovulation effect on preimplantation development can be discussed.

• Molecules and Cells
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• v.41 no.8
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• pp.781-798
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• 2018

Plants have evolved strategies to cope with drought stress by maximizing physiological capacity and adjusting developmental processes such as flowering time. The WOX13 orthologous group is the most conserved among the clade of WOX homeodomain-containing proteins and is found to function in both drought stress and flower development. In this study, we isolated and characterized OsWOX13 from rice. OsWOX13 was regulated spatially in vegetative organs but temporally in flowers and seeds. Overexpression of OsWOX13 (OsWOX13-ov) in rice under the rab21 promoter resulted in drought resistance and early flowering by 7-10 days. Screening of gene expression profiles in mature leaf and panicles of OsWOX13-ov showed a broad spectrum of effects on biological processes, such as abiotic and biotic stresses, exerting a cross-talk between responses. Protein binding microarray and electrophoretic mobility shift assay analyses supported ATTGATTG as the putative cis-element binding of OsWOX13. OsDREB1A and OsDREB1F, drought stress response transcription factors, contain ATTGATTG motif(s) in their promoters and are preferentially expressed in OsWOX13-ov. In addition, Heading date 3a and OsMADS14, regulators in the flowering pathway and development, were enhanced in OsWOX13-ov. These results suggest that OsWOX13 mediates the stress response and early flowering and, thus, may be a regulator of genes involved in drought escape.

• Journal of Communications and Networks
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• v.18 no.3
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• pp.364-374
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• 2016

With vast amounts of spectrum available in the millimeter wave (mmWave) band, small cells at mmWave frequencies densely deployed underlying the conventional homogeneous macrocell network have gained considerable interest from academia, industry, and standards bodies. Due to high propagation loss at higher frequencies, mmWave communications are inherently directional, and concurrent transmissions (spatial reuse) under low inter-link interference can be enabled to significantly improve network capacity. On the other hand, mmWave links are easily blocked by obstacles such as human body and furniture. In this paper, we develop a multi-hop relaying transmission (MHRT) scheme to steer blocked flows around obstacles by establishing multi-hop relay paths. In MHRT, a relay path selection algorithm is proposed to establish relay paths for blocked flows for better use of concurrent transmissions. After relay path selection, we use a multi-hop transmission scheduling algorithm to compute near-optimal schedules by fully exploiting the spatial reuse. Through extensive simulations under various traffic patterns and channel conditions, we demonstrate MHRT achieves superior performance in terms of network throughput and connection robustness compared with other existing protocols, especially under serious blockage conditions. The performance ofMHRT with different hop limitations is also simulated and analyzed for a better choice of the maximum hop number in practice.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.9A
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• pp.888-897
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• 2007

In this paper, we propose an uplink dynamic resource allocation algorithm to increase sector throughput and fairness among users in 802.16e OFDMA TDD system. In uplink, we address the difference between uplink and downlink channel state information in 802.16e OFDMA TDD system. The simulation results show that not only an increment of 10% of sector throughput but higher level of fairness is achieved by round-robin using the FLR and the rate / margin adaptive inner closed-loop power control algorithm. The FLR algorithm determines the number of sub-channels to be allocated to the user according to the user's position. Also, we get 31.8% more sector throughput compared with the round-robin using FLR by FASA algorithm using uplink channel state information. User selection, sub-channel allocation, power allocation algorithms and simulation methodology are mentioned in Part I.