Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Monitoring the Differentiation and Migration Patterns of Neural Cells Derived from Human Embryonic Stem Cells Using a Microfluidic Culture System

  • Lee, Nayeon (CHA Stem Cell Institute, CHA University) ;
  • Park, Jae Woo (Division of World Class University Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University) ;
  • Kim, Hyung Joon (Division of World Class University Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University) ;
  • Yeon, Ju Hun (Division of World Class University Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University) ;
  • Kwon, Jihye (CHA Stem Cell Institute, CHA University) ;
  • Ko, Jung Jae (CHA Stem Cell Institute, CHA University) ;
  • Oh, Seung-Hun (CHA Stem Cell Institute, CHA University) ;
  • Kim, Hyun Sook (CHA Stem Cell Institute, CHA University) ;
  • Kim, Aeri (CHA Stem Cell Institute, CHA University) ;
  • Han, Baek Soo (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Sang Chul (Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeon, Noo Li (Division of World Class University Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University) ;
  • Song, Jihwan (CHA Stem Cell Institute, CHA University)
  • Received : 2014.05.26
  • Accepted : 2014.05.29
  • Published : 2014.06.30
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Abstract

Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.

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References

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