• Development and Reproduction
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• v.22 no.3
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• pp.245-252
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• 2018

To improve survival rates of vitrified pig oocytes, the treatment of cytoskeletal stabilizer on an appropriate time is one of the possible approaches. However, the exact treatment timing and effect of cytoskeletal stabilizer such as cytochalasin B (CB) is not well known during oocyte vitrification procedures. Thus, the present study was conducted to determine optimal treatment timing of CB during vitrification and warming procedures. In experiment 1, the survival rates of the postwarming pig oocytes were analyzed by fluorescein diacetate (FDA) assays with 4 classifications. In results, post-warming oocytes showed significantly (p<0.05) decreased number of alive oocytes (31.8% vs. 86.4%) compared to fresh control. In detail, the significant difference (p<0.05) was found only in strong fluorescence (18.2% vs. 70.5%) not in intermediate fluorescence groups (13.6% vs. 15.9%). In experiment 2, CB was treated before (CB-Vitri) and after (Vitri-CB) vitrification. In results, group of Vitri-CB showed significantly (p<0.05) higher (91.6%) survival rates compared to group of CB-Vitri (83.7%), significantly (p<0.05) and comparable with group of Vitri Control (88.7%) by morphological inspection. In FDA assay results, group of Vitri-CB showed significantly (p<0.05) higher (44.2%) survival rates compared to groups of CB-Vitri (36.7%) and Vitri Control (35.1%). In conclusion, the increased survival rates of post-warming pig oocyte treated with Vitri-CB method are firstly described here. The main finding of present study is that the CB treatment during recovery could be helpful to refresh the post-warming pig oocyte resulting its improved survival rates.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.69-69
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• 2003

We took the nationwide survey of three main honeybee diseases, European foulbrood (EFB; Melissococcus pluton), chalk brood (CB; Ascosphera apis), and nosema (Nosema spp.) in 2001 and 2002 from South Korea. The number of infected apiaries with EFB and CB examined from 21 apiaries were 9 and 13, respectively. The average percentages of infectedcolonies in apiaries where EFB and CB occurred were 7.4% and 12.8%. (omitted)

• Journal of Industrial Convergence
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• v.18 no.5
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• pp.1-13
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• 2020

The purpose of this study is to find out how much the investment effect of convertible bond(CB) is from the perspective of investors and to present efficient investment plans to investors. The research method is to investigate the coupon interest rate, maturity interest rate, conversion price, etc. for CBs. As a result of the study, it was analyzed that CB's investment efficiency was low because the conversion price excess days ratio was only about 1/4 of the conversion date. The conversion day yield was -6.3% and the maturity day yield was -5.2% on average. It was analyzed that the number of stocks with negative conversion day yield was 2.4 times higher than the number of positive stocks and 3.7 times higher than the number of positive stocks with a maturity day yield, so the expected return on equity conversion of CB was low.

• The Korean Journal of Food And Nutrition
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• v.29 no.2
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• pp.178-185
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• 2016

This study was designed to develop and to qualify a coffee alternative beverage using a mixture of coffee beans and roasted black beans (Rhynchosia nulubilis). Therefore, the total isoflavone content (TIC), total phenol content (TPC), antioxidant activity, anti-inflammatory activity, NFATc1 (Nuclear factor of activated T-cells c1) expression in RANKL (receptor activator of nuclear factor kappa-B ligand)-stimulated RAW264.7 cells and sensory evaluation were measured for 5 different Cb (coffee bean)-RoS (roasted seomoktae) mixture extracts (Cb100RoS0, Cb75RoS25, Cb50RoS50, Cb25RoS75, and Cb0RoS100). Cb0RoS100 had the highest TIC ($516.83{\pm}36.61mg/100g$) and TPC ($18.11{\pm}1.77mg$ TAE/100 g) along with the highest antioxidant activity as measured by DPPH radical scavenging activity ($73.55{\pm}8.11%$) and ABTS radical scavenging activity ($63.27{\pm}7.27%$). Also, Cb0RoS100 showed the highest anti-inflammatory activity as measured by NO production ($13.57{\pm}2.21{\mu}M$) and PGE2 production ($3.25{\pm}0.21ng/mL$). The more the RoS ratio was increased in the mixtures of Cb-RoS, the more the NFATc1 protein expression was decreased in RANKL-stimulated RAW264.7 cells. In case of sensory evaluation, Cb50RoS50 had the highest scores for flavor, delicate flavor and overall quality, which were similar to those in Cb alone (Cb100RoS0). We suggest that the use of RoS replacement instead of Cb in/as a coffee alternative beverage may help to reduce the risk of caffeine-related bone loss and/or bone disease by effectively blocking NFATc1 expression in RANKL-stimulated RAW264.7 cells compared with Cb alone.

• Animal cells and systems
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• v.13 no.4
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• pp.371-379
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• 2009

The endocannabinoid system (ECS) plays a key role in the regulation of appetite, body weight and metabolism. We undertook the present study to further clarify the regulation of the cannabinoid CB1 receptor (CB1, CNR1) in human adipose tissue in obesity. CB1 receptor mRNA expression was ~1.6-fold (p<0.004) and 1.9-fold higher (P<0.05) in subcutaneous adipocytes from obese compared to non-obese subjects in microarray and quantitative real-time PCR studies, respectively. Higher CB1 receptor mRNA expression levels in both adipose tissue (~1.2 fold, P<0.05) and adipocytes (~2 fold, P<0.01) were observed in samples from visceral compared to subcutaneous depots collected from 22 obese individuals. Immunofluorescence confocal microscopy demonstrated the presence of CB1 receptor on adipocytes and also adipose tissue macrophages. These data indicate that adipocyte CB1 receptor is up-regulated in human obesity and visceral adipose tissue and also suggest a potential role for the ECS in modulating immune/inflammation as well as fat metabolism in adipose tissue.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.22 no.2
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• pp.45-55
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• 2017

Long chain alkyl diols (LCDs) have been reported in sediments from various marine environments. Rampen et al. (2012) introduced the paleo-sea surface temperature (SST) proxy, Long chain Diol Index (LDI) based on the relative abundance of $C_{30}$ 1,15-diol, $C_{28}$ 1,13-diol, and $C_{30}$ 1,13-diol. In general, CP-Sil5CB and DB-5ms columns have been used for the quantitative and qualitative analysis of LCDs with a GC-MS. In this study, we examined the effect of three different columns (CP-Sil5CB, HP-5ms and DB-5) on the quantitative analysis of LCDs using marine sediments from the East Sea of Korea and the western Arctic Ocean. In general, our study showed that the results of CP-Sil5CB differed significantly from those of HP-5ms and DB-5. However, the differences of the LDI-derived SSTs among three columns were $0.1-0.2^{\circ}C$ for the East Sea and $0.2-0.7^{\circ}C$ for the western Arctic Ocean, which were well within the calibration error range (${\pm}1{\sigma}$). Accordingly, our study showed that the use of different columns resulted in significant differences of LCDs concentrations, but its effect on the LDI was relatively insignificant. Therefore, it appears that the different columns can be used for the paleo-SST reconstruction in the East Sea and the western Arctic Ocean using the LDI proxy.

• Korean Journal of Pharmacognosy
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• v.46 no.3
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• pp.229-233
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• 2015

The anti-nociceptive effect of an ethanol extract and its various solvent fractions from Curcuma longa Linne ethanol extract was studied using the writhing test in mice. Different fractions by various solvent extraction from Curcuma longa Linne ethanol extract were administered orally 1 hr or time-course (0.5, 1, 2 and 5 hr) before intraperitoneal injection of acetic acid. After treatment with 30% ethanol extract and n-butanol fraction, CB-1, at a dose of 250 mg/kg, the significant writhing responses were 87.5 ± 13.4 (inhibition rate 31%, p<0.01) and 75.1 ± 11.1 (inhibition rate 41%, p<0.01) lower than the control group. At the dose of CB-1 50 mg/kg and 250 mg/kg, CB-1 showed a similar activity comparing to diclofenac of 10 mg/kg. A time-course experiment was performed, which involved oral administration of CB-1 (250 mg/kg) at 0, 0.5, 1, 2, and 5 hr before acetic acid intraperitoneal injection. The most effective time of CB-1 was 30 min before treatment and persisting until 2 hr. This study showed that Curcuma longa Linne has anti-nociceptive properties comparable with those of diclofenac, which suggests promise for the treatment of intractable visceral pain in humans. Major components of the active fraction are identified as curcumin, cyclocurcumin and demethoxycurcumin.

• Geomechanics and Engineering
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• v.35 no.1
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• pp.13-27
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• 2023

The model production for large-scale (lateral length ≥ 2.0 m) capillary barrier (CB) model tests is time and cost-intensive. To address these limitations, the framework of a small-scale CB (SSCB) model test under the lateral no-flow condition has been established. In this study, to validate the experimental methodology of the SSCB model test, a series of seepage analyses on the SSCB model test and engineered slopes in the same and additional test conditions was performed. First, the seepage behavior and diversion length (L_D) of the CB system were investigated under three rainfall conditions. In the seepage analysis for the engineered slopes with different slope angles and sand layer thicknesses, the L_D increased with the increase in the slope angle and sand layer thickness, although the increase rate of the L_D with the sand layer thickness exhibited an upper limit. The L_D values from the seepage analysis agreed well with the results estimated from the laboratory SSCB mode test. Therefore, it can be concluded that the experimental methodology of the SSCB model test is one of the promising alternatives to efficiently evaluate the water-shielding performance of the CB system for an engineered slope.

• The Bulletin of The Korean Astronomical Society
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• v.30 no.2
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• pp.45.1-45.1
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• 2005

• The Bulletin of The Korean Astronomical Society
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• v.31 no.2
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• pp.72.1-72.1
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• 2006