• Title/Summary/Keyword: Bio-nano

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Genetic Characterization of Encephalomyocarditis Virus Isolated from Aborted Swine Fetus in Korea

  • Song, Min-Suk;Joo, Young-Ho;Lee, Eun-Ho;Shin, Jin-Young;Kim, Chul-Jung;Shin, Kwang-Soon;Sung, Moon-Hee;Choi, Young-Ki
    • Journal of Microbiology and Biotechnology
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    • v.16 no.10
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    • pp.1570-1576
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    • 2006
  • An encephalomyocarditis virus (EMCV-CBNU) was isolated from an aborted swine fetus in October 2005. To investigate the genetic origin and virulence of the EMCV-CBNU strain, we determined the complete sequence of the virus and tested its virulence in mice. Genetic characterization revealed that the RNA genome was composed of 7,713 nucleotides with a single open reading frame (2,292 amino acids), coding 12 proteins. The EMCV-CBNU had the shortest poly(C) tract, consisting of 10 C's ($C_{10}$), compared with all the other EMCV strains reported in GenBank. Amino acid and phylogenetic analyses showed that EMCV-CBNU had the highest genetic identity with strain 2887A (99.7%), which was originally isolated from a fetus in a pig breeding farm that had a history of reproductive failure. Because rodents are the natural host of EMCV, we investigated the virulence of EMCV-CBNU in mice. Surprisingly, all mice inoculated with more than $1{\times}10^2\;TCID_{50}/0.1ml$ of EMCV-CBNU showed symptoms of hind limb paralysis and eventually died during 3 and 8 days postinoculation (DPI). Furthermore, when we inoculated the virus into pregnant mice, all dams and their fetuses died in 6 DPI. This is the first report on a full genomic analysis of swine EMCV in Korea, which exhibits high virulence in mice.

Leaching of Ca, Fe and Si in Electric Arc Furnace Steel Slag by Aqueous Acetic acid Solution for Indirect Carbonation (간접탄산염화를 위한 전기로제강슬래그 중 Ca, Fe 및 Si 성분의 초산수용액 침출)

  • Youn, Ki-Byoung
    • Resources Recycling
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    • v.26 no.1
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    • pp.37-42
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    • 2017
  • It has been reported that aqueous indirect carbonation process of calcium silicate mineral could be one of the most promising methods for $CO_2$ sequestration. The process consists of two main steps, extraction of Ca from calcium silicate and carbonation of the extracted solution by $CO_2$. Many types of acids such as HCl and $HNO_3$ can be used in the extraction step of the process. In the case of using aqueous acetic acid solution as the extraction solvent, acetic acid can be reproduced at the carbonation step of the extracted solution by $CO_2$ and recycled to extraction step for reuse it. Industrial by-products such as iron and steel slags are potential raw materials of the indirect carbonation process due to their high contents of calcium silicate. In this study, in order to examine the extraction efficiency of domestic electric arc furnace steel slag by aqueous acetic acid solution, extraction experiments of the slag were performed by using the aqueous acetic acid solutions of varying extraction conditions ; acetic acid concentrations, extraction temperatures and times.

Effect of Adding Isopropylphenyl Diphenyl Phosphate on Isothermal Crystallization Behavior and Flame Retardancy of PLA Film (Isopropylphenyl Diphenyl Phosphate 첨가가 PLA필름의 등온결정화 거동과 방염특성에 미치는 영향)

  • Kim, Moon-Sun;Kim, Gyusun;Kim, Byung-Woo
    • Applied Chemistry for Engineering
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    • v.23 no.2
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    • pp.169-175
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    • 2012
  • In the study, the effects of $130{\sim}150^{\circ}C$ annealing condition and 1~10 wt% isopropylphenyl diphenyl phosphate (IPPP) on crystallization behavior and flame retardancy of a full name (PLA) film were determined. The crystallization kinetics of PLA films with adding 1, 5, and 10 wt% IPPP at $140^{\circ}C$ were higher than those at 130 and $150^{\circ}C$. The average crystallinity and crystallite size of PLA film with 1 wt% IPPP were 21.3% and 24.8 nm, respectively. With an increasing IPPP content, the crystallinity of PLA film increased and the crystallite size decreased. The burning rate lowered with an increasing IPPP content as well.

Effect of Slander Glasswort Extract Yogurt on Quality during Storage (함초 추출물 첨가가 요구르트 저장 중의 품질 특성에 미치는 영향)

  • Cho, Young-Sim;Kim, Soon-Im;Han, Young-Sil
    • Korean journal of food and cookery science
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    • v.24 no.2
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    • pp.212-221
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    • 2008
  • This study was performed in an effort to create a functional and stable yogurt product containing slander glasswort extract. The extract was added to milk at concentrations of 0.25, 0.5, and 1.0% (w/v), which was then fermented with lactic acid bacteria (Lactobacillus bulgaricus and Streptococcus thermophilus) at $42^{\circ}C$ for 6 hr. During a period of 15 days of storage, the quality characteristics of the yogurt samples were evaluated in terms of acid production (pH and titratable acidity), levels of lactic acid bacteria, color values, viscosity, and sensory characteristics. There were no significant differences in pH during the storage period; however, the control yogurt presented the highest pH value. The 1.0% slander glasswort yogurt had the highest titratable acidity; but again, there were no significant differences among the yogurts. The 1.0% slander glasswort yogurt also had the highest level of lactic acid bacteria, and both the control and slander glasswort-containing yogurts had increasing levels of lactic acid bacteria over the storage period. The 1.0% slander glasswort yogurt had the lowest L-value and highest a- and b-values. And the slander glasswort yogurts presented lower viscosity values than the control. In sensory evaluations, the 0.25% slander glasswort yogurt scored higher than the other groups for color, flavor, viscosity, sweetness, sourness, and overall palatability. The final sensory results indicated that the 0.25% slander glasswort yogurt was superior.

The effects of daily intake timing of almond on the body composition and blood lipid profile of healthy adults

  • Liu, Yanan;Hwang, Hyo-Jeong;Ryu, Hyesook;Lee, You-Suk;Kim, Hyun-Sook;Park, Hyunjin
    • Nutrition Research and Practice
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    • v.11 no.6
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    • pp.479-486
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    • 2017
  • BACKGROUND/OBJECTIVES: Timing of almond intake during a day may result differently in the perspectives of body composition and changes of lipid profile. The current study was conducted to compare the effects of daily almond intake as a preload versus as a snack on body composition, blood lipid profile, and oxidative and inflammation indicators among young Korean adults aged 20-39 years old. SUBJECTS/METHODS: Participants were randomly assigned to one of three groups: a pre-meal almond group (PM), a snack almond group (SN) in which participants were instructed to consume 56 g of almonds either as a preload before meals or as a snack between meals, respectively, and a control group (CL) in which participants were provided high-carbohydrate iso-caloric control food. Measurements were performed at baseline, weeks 8 and 16. RESULTS: A total of 169 (M 77 / F 92) out of the 227 participants completed the study between June 2014 and June 2015 (n = 58 for PM; 55 for SN; and 56 for CL). A significant decrease in body fat mass was observed in the PM group at both weeks 8 and 16 compared with the CL. There were significant intervention effects on changes of body fat mass (P = 0.025), body fat percentages (P = 0.019), and visceral fat levels (P < 0.001). Consuming almonds as a daily snack reduced the levels of total cholesterol (P = 0.043) and low-density lipoprotein (LDL) cholesterol (P = 0.011) without changing high-density lipoprotein (HDL) cholesterol compared with the CL. CONCLUSION: Almond consumption as a preload modified body fat percentages, whereas snacking on almonds between meals improved blood lipid profiles. This trial was registered at ClinicalTrials.gov as NCT03014531.

In-Bi-Sn Alloy Sheet for Thermal Fuse Element of Secondary Battery Safety System (이차전지온도퓨즈용 In-Bi-Sn계 가용합금박판 연구)

  • Youn, Ki-Byoung
    • Resources Recycling
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    • v.26 no.5
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    • pp.22-28
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    • 2017
  • In-Bi-Sn alloy sheet has been used as a thermal fusible parts of secondary battery safety system. This study offers a simple process to make In-Bi-Sn alloy fusible parts. The process consists of two procedures, melting and sheeting by tape casting. 62.5 wt%-In 20.0 wt%-Bi 17.5 wt%-Sn (M.P. $92.4^{\circ}C$) alloy sheet obtained by tape casting was used as the thermal fusible sheet of thermal fuse system for mobile telephone. The performance test of the system was carried out in oil bath, and the fusible alloy sheet was melted and cut off at $95^{\circ}C$. This results confirmed the possibility that the alloy sheet obtained by tape casting can be usable as a thermal fusible parts of battery safety system. And this process can be applied as a simple process to recycle the In-Bi-Sn alloy scrap separated from the used thermal fuse system.

Metallurgical Observation of the Buddhist Bell of Youngmoon Mountain Sangwonsa Temple (용문산 상원사 범종의 금속학적 고찰)

  • Doh, Jungmann;Park, Bangju;Lee, Jungil;Hong, Kyungtae
    • Korean Journal of Metals and Materials
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    • v.50 no.11
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    • pp.829-838
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    • 2012
  • The microstructure, chemical composition, and lead isotope ratio of the Buddhist bell of Yongmoon Mountain Sangwonsa temple, which was selected as one of the three great bells of Korea by Japanese historians, were analyzed in order to estimate the origin of the material and the time of casting. The microstructure of the temple bell was composed of a copper matrix phase with ${\alpha}$, a face centered cubit lattice structure, a ${\delta}$ phase with $Cu_{41}$ $(Sn,Ag,Sb)_{11}$ as the chemical structural formula, dispersed lead and $Cu_2S$ particles, and locally agglomerated fine particles. Through analysis of the chemical composition of the bell, a criterion (Pb: 0-3.0 wt%, Sn: 10-15 wt%) for distinguishing the bells of the Shilla dynasty from the bells of the Koryo Chosun dynasty is proposed. Examining the lead isotope ratio of $^{207}Pb/^{206}Pb$ and $^{208}Pb/^{206}Pb$ of the Buddhist bell of Sangwonsa temple proved that the bell was fabricated using raw materials in South Korea, which led to the conclusion that the bell was cast in Korea and the top board of the bell has been damaged by an unknown individual. The criteria of distinguishing the bells from the Shilla dynasty from the bells of the Koryo Chosun dynasty presented for the first time in this research is expected to aid in identifying and estimating the previously unclear production years of other bells.

Management of Food Allergy in the Facilities Registered at Center for Children's Foodservice Management in Gangdong-gu (강동구 어린이급식관리지원센터 등록 시설의 식품알레르기 관리 현황)

  • Kim, Soon Mi
    • Korean Journal of Community Nutrition
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    • v.26 no.5
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    • pp.396-407
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    • 2021
  • Objectives: We examined the common allergenic foods, symptoms and management of food allergies in children attending the facilities registered at Center for Children's Foodservice Management in Gangdong-gu, Korea. Methods: The survey was conducted among the directors or head teachers of 186 children's food service facilities with 7,591 children in 2019. The questionnaire consisted of three parts, including general information about food service facilities, information related to food allergies and allergenic foods and symptoms in individual children. Results: The number of children with food allergy was 271 (3.6%), and the proportion decreased with the increase of age. There were 91 children (33.6%) with a medical certificate, and these children had a significantly higher number of allergenic foods and symptoms than those without a medical certificate. Allergenic food groups included meat, fish, eggs and legumes (59.1%), fruits (12.4%), milk and dairy products (8.9%), cereals (7.8%), vegetables (6.2%), processed foods (3.8%) and oils and sugars (1.9%). Eggs accounted for 22.1%, followed by peanut and tree nuts (18.6%), fruits (12.4%), milk and dairy products (8.9%), shellfish (8.6%), vegetables (6.2%), fish (5.7%), cereals (4.3%) and meat (1.1%). The common allergenic foods were eggs, peanuts, walnuts, kiwi, shrimp, milk, tomatoes, mackerel, blue-green fish, peaches, shellfish (clams and abalone), buckwheat, wheat and soybeans. The most common allergic symptoms were skin and mucous membrane symptoms, such as hives, rash, itching and oral angioedema. Meal management for children with food allergies showed different trends depending on the causative food. Conclusions: The objective diagnosis by an allergist should be done for food allergy management in children's catering facilities. A system for systematic meal management of causative foods should be prepared.

Recovery of Tin with High Purity for Dental Materials from Waste Tin oxide by Reduction and Electro Refining (폐주석산화물로부터 환원공정 및 전해정련을 통한 치과용 고순도 주석 회수)

  • Jung, Hyun-Chol;Kim, Sang-Yeol;Lee, Min-Ho
    • Resources Recycling
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    • v.27 no.6
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    • pp.38-43
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    • 2018
  • In this study, using electro-refining process and methane gas reduction, we performed studying the recovery of tin with high purity from waste tin oxide had used as a electrode rod of ceramic furnace which occurred during glass production process. We recovered the crude tin of 99% purity from a methane gas reduction process and controlled a little amount of impurities. When the electrolytic refining condition was a current density of $60A/dm^2$ and the sulfuric acid concentration of 0.75 mol, 96.8% of recovered tin (99.979% of purity) were recovered during the electrolytic refining. We confirmed that toxic impurities such as Pb, Sb included in electrode rod. could be controlled.

Education Plan of Human Resources required in Convergence Industry of Medical and IT (의료 IT 융합 서비스 산업을 위한 인재 양성방안)

  • Woo, Sung-hee
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2014.05a
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    • pp.844-847
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    • 2014
  • 'Healthcare 3.0' paradigm, which comes from the convergence of medical and department of ICT(Information and Communication Technology), meets the government's 'creative economy'. It has emerged as new higher value-added markets and industries, created jobs, and got the 'land of opportunity'. Despite of the global recession, the health care market in the world from 2009 to now has earned a steady growth. The annual growth rate in domestic industrial scale is expected to more than 10% higher based on 153 trillion won in 2010. In particular, combination of health care and ICT, BT(BioTechnology), NT(NanoTtechnology), which is so called 'super-convergence', leap in the health care industry, is expected to open up a new paradigm. In addition, by 2020 all of the health care industry, the IT convergence industries account for the largest proportion, and more than 50,000 of the employment are to be expected. In this study, we survey and analyze problems of medical and IT convergence technology, the future of the medical and health care industry, the demanded healthcare IT staff, finally suggest education plan of human resources required in convergence industry of medical and IT.

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