• Molecules and Cells
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• v.39 no.9
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• pp.687-691
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• 2016

Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous recombination in bacteria. Although the methods for TALE assembly are constantly improving, these methods are not convenient because of laborious assembly steps or large module libraries, limiting their broad utility. To overcome the barrier of multiple assembly steps, we report a one-step system for the convenient and flexible assembly of a 180 TALE module library. This study is the first demonstration to ligate 9 mono-/dimer modules and one circular TALEN backbone vector in a one step process, generating 9.5 to 18.5 repeat sequences with an overall assembly rate higher than 50%. This system makes TALEN assembly much simpler than the conventional cloning of two DNA fragments because this strategy combines digestion and ligation into one step using circular vectors and different modules to avoid gel extraction. Therefore, this system provides a convenient tool for the application of TALEN-mediated genome editing in scientific studies and clinical trials.

• IE interfaces
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• v.22 no.2
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• pp.144-152
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• 2009

Assembly line has been recognized as an efficient production system in mass production. However, the recent production environment characterized as mass customization urges production managers to transform a long assembly line to a number of short assembly cells. To maximize the utilization of resources in an assembly cell, it is important to have the line balanced. This paper presents a bucket brigade-based assembly cell. Bucket brigade is a way of coordinating workers who progressively perform a set of assembly operations on a flow line. Each worker follows a simple rule: perform assembly operations on a product until the next worker downstream takes it over; then go back to the previous worker upstream to take over a new assembly job. In this way, the line balances itself. The bucket brigade assembly cell is analyzed and compared with traditional assembly lines and general assembly cells. The paper also discusses some prerequisite requirements and limitations when the bucket brigade assembly cells are employed.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.6 s.261
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• pp.635-643
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• 2007

As many engineers and technicians are involved in the design process of large scale and/or complex products, there are a lot of miss matches and interferences due to designers' faults and several kinds of CAD systems. Recently, CAD systems are applied to verify and check the assembly process. Digital Mock-Up(DMU) system, a tool to build a virtual mock-up in the design stage, has been used to prevent the interferences and miss matches during precision design processes. Using the virtual assembly tool, engineers are able to design precision and interference free parts without physical mock-ups. Instead of a single CAD source, several CAD systems are used to design a complex product. Several organizations are involved in the distributed design environment for heterogeneous multi-CAD assembly. XML and the lightweight CAD file are proposed for the multi-CAD assembly. XML data contains hierarchy of the heterogenenous multi-CAD assembly. STEP PDM schema and STEP ISO 10303-28 formations are applied to construct the XML data. The lightweight CAD file produced from various CAD files through ACIS kernel and InterOp not only contains mesn, B-Rep and topological data, but also is used to visualize CAD data and to verify dimensions. Developed system is executed on the desktop computers. It does not require commercial CAD systems to visualize 3D assembly data. Real-time interference and fitness checks, dimensional verification, and design and assembly verification are performed on the developed system. Assembly of heterogeneous models for a car is conducted to verify the effectiveness of the developed DMU system on the Internet.

• Journal of Korean Society of Archives and Records Management
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• v.12 no.1
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• pp.141-165
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• 2012

Since 'the National Assembly Electronic Document System' was introduced in September 2004, the amount of electronic records production in the Nation Assembly has been on a rapid increase. The number of electronic records which 'the National Assembly Records Management System' took over until September 2011 is '240,000', 72% of the total transferred records. However, the National Assembly's electronic records management has a number of serious problems concerning electronic records long-term management and preservation. In the context, this study aims at discussing alternative strategies for effective management and preservation of the Nation Assembly electronic records. In the theoretical aspect, it reviews 'parliament records' definition and management trend. As a case study, it explores the present state of 'the National Assembly Records Management System' ranging from legal aspects to technical aspects. Based on these researches the study critically analyzes the Nation Assembly's electronic records management in comparison with overseas cases. As a result it provides alternative plans of four major areas, including law, management body, management object and technical system, to improve the National Assembly's electronic records management.

• Korean Journal of Computational Design and Engineering
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• v.9 no.4
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• pp.325-333
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• 2004

To ensure competitiveness in the modern automotive market, material arrangements and information managements should be performed concurrently with new car developments. In automotive general assembly shops, thus, new business workflow and supporting environments are inevitable to reduce the manufacturing preparation time in developing a new car in the manner of concurrent and collaborative engineering. Since complete material arrangements for a whole general assembly system is a huge and complex job, several planners should execute their planning jobs and share information. Therefore, each planner should provide others with his/her results with continuous on-line communication and cooperation. Digital automotive general assembly factory is useful the performing concurrent and collaborative engineering and is essential for material arrangements and information managements systems. In this research, we constructed a sophisticated digital factory of an automotive general assembly shop by measuring and modeling through the parametric 3-D CAD, and a web-based system for concurrent and collaborative material arrangements for automotive general assembly via 3D mock-up is developed. By the digital general assembly shop and developed web-based system, savings in time and colt of manufacturing preparation activities are possible, and the reliability of the planning result Is greatly improved.

• Journal of Korean Society for Quality Management
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• v.49 no.1
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• pp.17-29
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• 2021

Purpose: During 2015-2019, the average amount of political donation to the national assembly members in Korea was 1,000 won per person. Despite its benefits such as receiving tax credits, the donation system has not been actively practiced. This paper aims to promote political donations by suggesting a recommendation system of national assembly members by analysing the bills they proposed. Methods: In this paper, we propose a recommendation system based on two aspects: how similar the newly proposed or ammended bills are to the sponsors' interest (similarity index) and how much effort national assembly members put into those bills (intensity index). More than 25,000 bills were used to measure the recommendation quality index consisted with both the similarity and the intensity indices. Word2vec was used to calculate the similarity index of the bills proposed by the national assembly member to the sponsor's interest. The intensity index is calculated by diving the number of newly proposed or entirely revised bills with the number of senators who took part in those bills. Subsequently, we multiply the similarity index by the intensity index to obtain the recommendation quality index that can assist sponsors to identify potential assembly members for their donation. Results: We apply the proposed recommendation system to personas for illustration. The recommendation system showed an average f1 score about 0.69. The analysis results provide insights in recommendation for donation. Conclusion: n this study, the recommendation system was proposed to promote a political donation for national assembly members by creating the recommendation quality index based on the similarity and the intensity indices. We expect that the system presented in this paper will lower user barriers to political information, thereby boosting political sponsorship and increasing political participation.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.707-710
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• 1995

Structure of product, which is not considered by easy of assembly, has many hidden costs. For reduction of hidden costs, concepts of line balancing and assembly-oriented design are used. We find that part of structure can be improved by concept of line balancing. To reduce assembly time and enhance easy of assembly, design principles are developed by concept of assembly-oriented design. And design principles for improvement are applied according to condition of the grasped parts of product structure. Thersfore, we could design assembly-oriented design for better structure and low cost.

• IE interfaces
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• v.12 no.4
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• pp.586-594
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• 1999

This paper introduces a production scheduling system for the block assembly shop in a shipbuilding company. We are to make a schedule for the assembly shop and related subassembly production shop. The objectives of the scheduling system are balancing of the workload of the subassembly shops as well as the assembly shop. There are a number of technological and resource constraints including assembly space restriction which is most important and critical resource in the shop. It is very hard and time consuming to consider the two problems, the workload balancing and the spatial allocation problem, simultaneously, and hence, we analyze the two problems independently. The first problem has already been introduced(고시근, 1996), and the overall appearance of the system and the spatial allocation algorithm are presented in this paper.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.1191-1194
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• 2003

At present, the fabrication of optical filter for optical communication mostly depends on handwork, and only a few companies are propelling semi-automatic systems compounded with automatic and hand-operated methods. The measurement evaluation of optical filters is not commonly useful, so it causes difficulties in the producing field. And the packaging process becomes a very unstable process because the filters are judged from the results that they are not measured and immediately packaged during that process. In this situation, the automatic assembly system of optical filter for communication is extremely important and has begun to make its appearance as the most necessary technology for developing optical communication component with high-functionality. In this paper, we constructed systems of assembly and performance evaluation for micro optical collimator. And by using that, we designed a system capable of performance evaluation and assembly of film filter of about 30 $\mu\textrm{m}$ thickness as well as optical filter for common communication.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.19 no.39
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• pp.63-73
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• 1996

With the success of flexible manufacturing systems (FMSs), flexible assembly systems (FASs) have been developed to automate factories further. As in a cellular FMS, a cellular FAS is considered as the most flexible and feasible assembly system configuration Because of the differences between manufacturing and assembly operation, the logic of cell formation and cell layout between a FMS and a FAS is not the same. Since the time for assembly operation is usually relatively short, the transfer time is thus very crucial for the performance of assembly systems. Therefore in assembly systems it is important to reduce the transfer time by sequencing operations efficiently and arranging machines like the sequences. The network-type layout is not only feasible for the machine arrangement based on operation sequences, but it has also layout flexibility. Therefore it is a reasonable layout configuration for cellular FASs. This paper presents a method for the cell layout based on the network-type layout in a cellular FAS design.